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Tissue References Search Tool

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Organoids
Mouse

Mouse, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.
Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
 6 , 2017
Cells
Lung organoids
Enzymes
Medium
DMEM/F12
File
Nikolic.2017.pdf (13.78 MB)
Organoids
Mouse

Mouse

O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S.
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. ,
Bio Protoc
 6 , 2016
Cells
Intestinal organoids
Enzymes
Medium
Basal Medium
File
11657.pdf (395.41 KB)
Organoids
Mouse

Mouse, 8-12 week

Huch, M. , Bonfanti, P. , Boj, S. , Sato, T. , Loomans, C. , Van de Wetering, M. , Sojoodi, M. , Li, V. , Schuijers, J. , Gracanin, A. , Ringnalda, F. , Begthel, H. , Hamer, K. , Mulder, J. , Van Es, J. and De Koning, E.
Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis. ,
EMBO J
 32 , 2708-21 , 2013
Cells
Pancreas organoid
Enzymes
Neutral Protease : 0.012%
Collagenase : 0.012%
Medium
DMEM
File
11021.pdf (2.37 MB)
Organoids
Mouse

Mouse

Xue, X. and Shah, Y.
In Vitro Organoid Culture of Primary Mouse Colon Tumors. ,
J Vis Exp
 , e50210 , 2013
Cells
Colon tumor organoids
Enzymes
Medium
DMEM
Organoids
Mouse

Mouse

Barthel, E. , Speer, A. , Levin, D. , Sala, F. , Hou, X. , Torashima, Y. , Wigfall, C. and Grikscheit, T.
Tissue Engineering of the Intestine in a Murine Model. ,
J Vis Exp
 70 , e4279 , 2012
Cells
Intestinal organoids
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM
File
10893.pdf (556.52 KB)
Pancreas
Bovine
Bovine
Cotton, C. and Al-Nakkash, L.
Isolation and Culture of Bovine Pancreatic Duct Epithelial cells ,
Am J Physiol
 272 , G1328 , 1997
Cells
Duct epithelial
Enzymes
Collagenase : 0.1%
Medium
HEPES
Pancreas
Bovine
Bovine (Bos taurus)
Sato, T. , Sato, M. , Hudson, E. and Jones, R.
Characterization of Bovine Pancreatic Ductal Cells Isolated by a Perfusion-Digestion Technique ,
In Vitro
 19 , 651 , 1983
Cells
Ductal
Enzymes
Medium
EBSS
Pancreas
Bovine
Bovine
Stiles, G. and Lefkowitz, R.
Hormone-Sensitive Adneylate Cyclase ,
J Biol Chem
 257 (11) , 6287 , 1982
Cells
Platelets
Enzymes
Medium
(see reference)
Pancreas
Canine
Canine
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Pancreas
Canine
Canine, adult mongrel, 15-25 kg
Noel, J. , Rabinovitch, A. , Olson, L. , Kyriakides, G. , Miller, J. and Mintz, D.
A Method for Large-Scale, High-Yield Isolation of Canine Pancreatic Islets of Langerhans ,
Metabolism
 31 (2) , 184 , 1982
Cells
Islets
Enzymes
Collagenase Type 4 : 600-1100 u/ml
Deoxyribonuclease I : 10 ug/ml
Medium
RPMI 1640
Pancreas
Fish
Fish, Osphronemus gourami, 3-month-old, 0.5 mg
Schrezenmeir, J. , Laue, C. , Sternheim,E. , Wolbert, K. , Darquy, S. , Chicheportiche, D. , Kirchgessner, J. and Reach, G.
Long-Term Function of Single-Cell Preparations of Piscine Principal Islets in Hollow Fibers ,
Transplant Proc
 24 (6) , 2941 , 1992
Cells
Islets
Enzymes
Collagenase : 0.12 - 0.46 u/ml
Medium
RPMI 1640
Pancreas
Guinea-Pig
Guinea-pig
Schultz, G. , Sarras, Jr, M. , Gunther, G. , Hull, B. , Alicea, H. , Gorelick, F. and Jamieson, J.
Guinea Pig Pancreatic Acini Prepared with Purified collagenase ,
Exp Cell Res
 130 , 49 , 1980
Cells
Acinar
Enzymes
Collagenase Type 3 : 60 u/ml
Medium
Kreb's Ringer
Pancreas
Guinea-Pig
Guinea-pig, Hartley, albino, male, 350-400 g
Gardner, J. , Conlon, T. , Klaeveman, H. , Adams, T. and Ondetti, M.
Action of Cholecystokinin and Cholinergic Agents on Calcium Transport in Isolated Pancreatic Acinar Cells ,
J Clin Invest
 56 , 366 , 1975
Cells
Acinar
Enzymes
Medium
Kreb's Ringer
Pancreas
Guinea-Pig
Guinea-pig
Amsterdam, J. and Jamieson, J.
Structural and Functional Characterization of Isolated Pancreatic Exocrine Cells ,
Proc Natl Acad Sci U S A
 69 (10) , 3028 , 1972
Cells
Exocrine
Enzymes
Hyaluronidase : 0.15% - 0.2%
Medium
Kreb's Ringer
Pancreas
Hamster
Hamster
Feldman, J. and Chapman, B.
Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique ,
Acta Diabetol
 12 , 208 , 1975
Cells
Islets
Enzymes
Collagenase Type 4 : 1.3% - 2.0%
Medium
HBSS
Pancreas
Human
Human
Brissova, M. , Haliyur, R. , Saunders, D. , Shrestha, S. , Dai, C. , Blodgett, D. , Bottino, R. , Campbell-Thompson, M. , Aramandla, R. , Poffenberger, G. , Lindner, J. , Pan, F. , von Herrath, M. , Greiner, D. and Shultz, L.
α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes. ,
Cell Rep
 22 , 2667-2676 , 2018
Cells
Islets
Enzymes
Collagenase Type 1 : 1,600u/isolation
Neutral Protease : 200 u/isolation
Deoxyribonuclease I : 12,000 u/isolation
Medium
RPMI
File
11700.pdf (3.9 MB)
Pancreas
Human
Human
Cane, M. , Sutton, R. and Criddle, D.
Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas ,
Pancreapedia
 20 , 1 , 2011
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
See Reference
File
10888.pdf (362.98 KB)
Pancreas
Human
Human
Li, C. , Heidt, D. , Dalerba, P. , Burant, C. , Zhang, L. , Adsay, V. , Wicha, M. , Clarke, M. and Simeone, D.
Identification of Pancreatic Cancer Stem Cells. ,
Cancer Res
 67 , 1030 , 2007
Cells
Pancreatic cancer stem cells
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
medium 199
Pancreas
Human
Human, 25-55 yrs old
(also porcine)
Contractor, H. , Johnson, P. , Chadwick, D. , Robertson, G. and London, N.
The Effect of UW Solution and its Components on the Collagenase Digestion of Human and Porcine Pancreas ,
Cell Transplant
 4 , 615 , 1995
Cells
Islets
Enzymes
Collagenase : 0.4%
Medium
HBSS
Pancreas
Human
Human
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Pancreas
Human
Human, adult organ donors
Warnock, G. , Rajotte, R. , Evans, M. , Ellis, D. , DeGroot, T. and Dawidson, I.
Isolation of Islets of Langerhans Following Cold Storage of Human Pancreas ,
Transplant Proc
 XIX (4) , 3466 , 1987
Cells
Islets
Enzymes
Collagenase : 0.6%
Medium
Eurocollins solution
Pancreas
Human
Human
Izumi, R. , Konishi, K. , Ueno, K. , Shimizu, K. , Hirosawa, H. , Takahashi, N. and Miyazaki, I.
Isolation of Human Pancreatic Islets from Cryopreserved Pancreas ,
Transplant Proc
 XVII , 383 , 1985
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Human
Human
Gray, D. , McShane, P. , Grant, A. and Morris, P.
A Method for Isolation of Islets of Langerhans from the Human Pancreas ,
Diabetes
 33 , 1055 , 1984
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Human
Human, infant, age 1 day-1 year
Sutherland, D. , Matas, A. , Steffes, M. and Najarian, J.
Infant Human Pancreas: A Potential Source of Islet Tissue for Transplantation ,
Diabetes
 25 (12) , 1123 , 1976
Cells
Islets
Enzymes
Collagenase : 170-210 u/ml
Medium
HBSS
Pancreas
Monkey
Monkey, 3-5 Kg
Scharp, D. , Murphy, J. , Newton, W. , Ballinger, W. and Lacy, P.
Application of an Improved Isolation Technique for Islet Transplantation in Primates and Rats ,
Transplant Proc
 7 , 739 , 1975
Cells
Islets
Enzymes
Hyaluronidase : 0.05%
Medium
HBSS
Pancreas
Mouse
Mouse
Low, L. , Zavortink, M. , Mitchell, J. , Gan, W. , Do, O. , Schwiening, C. , Gaisano, H. and Thorn, P.
Insulin Secretion from Beta Cells in Intact Mouse Islets is Targeted Towards the Vasculature ,
Diabetologia
 57 , 1655 , 2014
Cells
Islets
Enzymes
Medium
HBSS
File
11622.pdf (2.91 MB)
Pancreas
Mouse
Mouse
Koh, D. , Moody, M. and Jo, J.
Collection of Islets of Langerhans using an Equilibrium Method. ,
Biotechniques
 55 , 34-7 , 2013
Cells
Islets
Enzymes
Collagenase : 0.1-0.25%
Medium
HBSS
File
11010.pdf (2.35 MB)
Pancreas
Mouse
Mouse, 8-12 week
Huch, M. , Bonfanti, P. , Boj, S. , Sato, T. , Loomans, C. , Van de Wetering, M. , Sojoodi, M. , Li, V. , Schuijers, J. , Gracanin, A. , Ringnalda, F. , Begthel, H. , Hamer, K. , Mulder, J. , Van Es, J. and De Koning, E.
Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis. ,
EMBO J
 32 , 2708-21 , 2013
Cells
Pancreas organoid
Enzymes
Neutral Protease : 0.012%
Collagenase : 0.012%
Medium
DMEM
File
11021.pdf (2.37 MB)
Pancreas
Mouse
Mouse, embryonic
Greggio, C. , De Franceschi, F. , Figueiredo-Larsen, M. , Gobaa, S. , Ranga, A. , Semb, H. , Lutolf, M. and Grapin-Botton, A.
Artificial Three-Dimensional Niches Deconstruct Pancreas Development In Vitro. ,
Development
 140 , 4452-62 , 2013
Cells
Pancreatic progenitor
Enzymes
Neutral Protease : 0.125%
Medium
DMEM
File
11023.pdf (4.01 MB)
Pancreas
Mouse
Mouse
Bertera, S. , Balamurugan, A. , Bottino, R. , He, J. and Trucco, M.
Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin. ,
J Transplant
 2012 , 856386 , 2012
Cells
Islets
Enzymes
Collagenase : 0.2%
Medium
RPMI 1640
Pancreas
Mouse
Mouse
Ding, G. , Wang, F. , Shu, J. , Tian, S. , Jiang, Y. , Zhang, D. , Wang, N. , Luo, Q. , Zhang, Y. , Jin, F. , Leung, P. , Sheng, J. and Huang, H.
Transgenerational Glucose Intolerance with Igf2/H19 Epigenetic Alterations in Mouse Islet Induced by Intrauterine Hyperglycemia. ,
Diabetes
 61 , 1133-42 , 2012
Cells
Islet
Enzymes
Medium
RPMI-1640
File
11623.pdf (2.03 MB)
Pancreas
Mouse
Mouse, 10 week
Kobayashi, T. , Yamaguchi, T. , Hamanaka, S. , Kato-Itoh, M. , Yamazaki, Y. , Ibata, M. , Sato, H. , Lee, Y. , Usui, J. , Knisely, A. , Hirabayashi, M. and Nakauchi, H.
Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells. ,
Cell
 142 , 787 , 2010
Cells
Islets
Enzymes
Collagenase : 0.2%
Medium
RPMI 1640
Pancreas
Mouse
Mouse, 6-8 month
Taguchi, Y. , Tasaki, Y. , Terakado, K. , Kobayashi, K. , Machida, T. and Kobayashi, T.
Impaired Insulin Secretion from the Pancreatic Islets of Hypothyroidal Growth-Retarded Mice. ,
J Endocrinol
 206 , 195-204 , 2010
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Mouse
Mouse
Li, D. , Yuan, Y. , Tu, H. , Liang, Q. and Dai, L.
A Protocol for Islet Isolation from Mouse Pancreas. ,
Nat Protoc
 4 , 1649 , 2009
Cells
Islets
Enzymes
Collagenase : 1,000 u/ml
Medium
RPMI 1640
Pancreas
Mouse
Mouse
Carter, J. , Dula, S. , Corbin, K. , Wu, R. and Nunemaker, C.
A Practical Guide to Rodent Islet Isolation and Assessment. ,
Biol Proced Online
 11 , 3 , 2009
Cells
Islets
Enzymes
Collagenase : 0.14%
Medium
RPMI 1640
File
11054.pdf (644.09 KB)
Pancreas
Mouse
Mouse, adult, male
Ji, B. , Gaiser, S. , Chen, X. , Ernst, S. and Logsdon, C.
Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation. ,
J Biol Chem
 284 , 17488 , 2009
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.