Prostate

Species	Cells	Enzyme(s)	Medium	Reference
Human Human	Prostate stromal cells	Collagenase Type 1 : 0.1%	RPMI 1640	Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi. Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R , Am J Pathol 165 , 533-40 , 2004
Human Human	Prostatic cancer fibroblasts	Collagenase : 225 u/ml Hyaluronidase : 125 u/ml	RPMI 1640	Taylor, R. , Toivanen, R. , Frydenberg, M. , Pedersen, J. , Harewood, L. , Collins, A. , Maitland, N. and Risbridger, G. Human Epithelial Basal Cells are Cells of Origin of Prostate Cancer, Independent of CD133 Status. , Stem Cells 30 , 1087 , 2012
Human Human, fetal	Prostatic fibroblasts	Collagenase Type 1 : 0.125%	DMEM/F12	Levine AC, Liu XH, Greenberg PD, Eliashvili M, Schiff JD, Aaronson SA, Holland JF, Kirschenbaum A. Androgens induce the expression of vascular endothelial growth factor in human fetal prostatic fibroblasts , Endocrinology 139 , 4672-8 , 1998
Human Human, 52-56 yr	Prostatic stromal cells	Collagenase Type 1 : 0.2%	DMEM/F-12	Le Hanh, Arnold Julia T, McFann Kimberly K, Blackman Marc R. DHT and testosterone, but not DHEA or E2, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells , Am J Physiol/Endo 290 , E952-60 , 2006
Mouse Mouse, 2 week	Prostatic epithelial	Collagenase Type 3 : 170 u/ml	DMEM	Imamov Otabek, Morani Andrea, Shim Gil-Jin, Omoto Yoko, Thulin-Andersson Christina, Warner Margaret, Gustafsson Jan-Ake. Estrogen receptor beta regulates epithelial cellular differentiation in the mouse ventral prostate , Proc Natl Acad Sci U S A 101 , 9375-80 , 2004
Mouse Mouse, male	Prostatic stem	Collagenase Type 1 : 170 u/ml	DMEM	Dubey P, Wu H, Reiter RE, Witte ON. Alternative pathways to prostate carcinoma activate prostate stem cell antigen expression , Cancer Res 61 , 3256-61 , 2001
Mouse Mouse, 6-8 week	Prostate epithelial/stem	Collagenase Type 2 : 0.5% Trypsin : 0.05%	HBSS	Burger, P. , Gupta, R. , Xiong, X. , Ontiveros, C. , Salm, S. , Moscatelli, D. and Wilson, E. High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells. , Stem Cells 27 , 2220-8 , 2009