Eye | Worthington Biochemical

Species	Cells	Enzyme(s)	Medium	Reference
Bovine Bovine	Microvascular endothelial	Collagenase/Dispase : 0.1%	MEM	Bowman, P. , Betz, A. and Goldstein, G. Primary Culture of Microvascular Endothelial Cells From Bovine Retina , In Vitro 18 (7) , 626 , 1982
Bovine Bovine	Pericyte	Collagenase : 0.2%	DMEM	Bryan, B. and D'Amore, P. Pericyte Isolation and USe is Endothelial/Pericyte Coculture Models , Meth Enzymol 443 , 315 , 2008
Bovine Bovine	Retinal endothelial	Collagenase Type 4 : 0.05% Deoxyribonuclease I : 0.02% Pronase : 0.02%	DMEM	Terlizzi, V. , Kolibabka, M. , Burgess, J. , Hammes, H. and Harmsen, M. The Pericytic Phenotype of Adipose Tissue-Derived Stromal Cells Is Promoted by NOTCH2. , Stem Cells 36 , 240-251 , 2018
Chicken Chick embryo (also rat)	Retinal	Trypsin : 0.6%	DMEM	Seigel, G. The Golden Age of Retinal Cell Culture , Mol Vis 5 , 4 , 1999
Chicken Chick, embryo, 10-14 day	Flat, retina	Trypsin : 0.1%	Tyrode's solution, CMF	Moyer, M. , Bullrich, F. and Sheffield, J. Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina , In Vitro Cell Dev Biol 26 , 1073 , 1990
Chicken Chick, embryo, 6 day	Retinal cells	Trypsin : 0.005% Deoxyribonuclease I : 0.005%	DMEM/F12	Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G. Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions , Cell Death Differ 180 , 159-68 , 2005
Chicken Chick embryo	Corneal epithelia	Collagenase : 0.08% Trypsin : 0.08%	HBSS	Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H. ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent , Inv Ophthal Visual Sci 43 , 3181-9 , 2002
Fish Fish, zebrafish	Retinal	Papain : 10 u/ml	L-15	Diekmann, H. , Kalbhen, P. and Fischer, D. Characterization of Optic Nerve Regeneration using Transgenic Zebrafish. , Front Cell Neurosci 9 , 118 , 2015
Fish Goldfish	Retinal Bipolar	Papain : 30 u/ml	HEPES	Graffe, M. , Zenisek, D. and Taraska, J. A Marginal Band of Microtubules Transports and Organizes Mitochondria in Retinal Bipolar Synaptic Terminals. , J Gen Physiol 146 , 109-17 , 2015
Human Human	Corneal stroma stem	Collagenase Type 1 : 0.1%	DMEM	Sidney, L. , Branch, M. , Dua, H. and Hopkinson, A. Effect of Culture Medium on Propagation and Phenotype of Corneal Stroma-Derived Stem Cells. , Cytotherapy 17 , 1706-22 , 2015
Human Human	Retinal	PDS kit : see reference	EBSS	Menon, M. , Mohammadi, S. , Davila-Velderrain, J. , Goods, B. , Cadwell, T. , Xing, Y. , Stemmer-Rachamimov, A. , Shalek, A. , Love, J. , Kellis, M. and Hafler, B. Single-cell Transcriptomic Atlas of the Human Retina Identifies Cell Types Associated with Age-related Macular Degeneration. , Nat Commun 10 , 4902 , 2019
Human Human	Muller glia	PDS kit : per instructions	DMEM	Xu, N. , Chen, Y. , Dean, K. , Lu, X. , Liu, X. , Wang, W. , Dean, D. , Kaplan, H. , Gao, L. , Dong, F. and Liu, Y. Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation. , Stem Cells 35 , 1579-1591 , 2017
Human Human	Corneal stromal keratocytes	Collagenase Type 1 : 0.1%	DMEM/F-12	Yusoff,B, Riau, A. , Yam, G. , Halim, N. and Mehta, J. Isolation and Propagation of Human Corneal Stromal Keratocytes for Tissue Engineering and Cell Therapy. , Cells 11 , 178 , 2022
Human Human, 5-65 years	Retinal pigment epithelial (RPE)	Trypsin : 0.25%	HBSS	Von Recum, H. , Okano, T. , Kim, S. and Bernstein, P. Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture , Exp Eye Res 69 , 97 , 1999
Human Human	Corneal limbal epithelial sheet	Neutral Protease : 5%	see reference	Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R. and Tseng SC Novel enzymatic isolation of an entire viable human limbal epithelial sheet , Inv Ophthal Visual Sci 44(10) , 4275 , 2003
Human Human, 18-68 yr	Corneal endothelial	Collagenase : 0.2% Neutral Protease : 1.0%	DMEM/F12	Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G. A novel method of isolation, preservation, and expansion of human corneal endothelial cells , Inv Ophthal Visual Sci 48 , 614-20 , 2007
Human Human	Corneal stromal stem	Neutral Protease : 1.2 u/ml Collagenase : 0.1%	DMEM	Du, Y. , Roh, D. , Funderburgh, M. , Mann, M. , Marra, K. , Rubin, J. , Li, X. and Funderburgh, J. Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro. , Mol Vis 16 , 2680 , 2010
Monkey Monkey, cynomolgus, young adult	Conjunctival lymphocytes	Collagenase Type 1 : 0.02%	RPMI 1640	Whittum-Hudson JA, Taylor HR. Antichlamydial specificity of conjunctival lymphocytes during experimental ocular infection , Infect Immun 57 , 2977 , 1989
Monkey Macaques and baboon	Retinal	Papain : 20-40 u/ml Deoxyribonuclease I : 400 u/ml	Ames' solution	Han ,Y. , Jacoby, R. and Wu, S. Morphological and Electrophysiological Properties of Dissociated Primate Retinal Cells. , Brain Res 875 , 175 , 2000
Mouse Mouse	Vitreous cells	Collagenase : 0.1% Hyaluronidase : 0.03%	DMEM	Iqbal, N. , Xu, L. , Devitt, C. and Skapek, S. Isolation and Characterization of Mammalian Cells Expressing the Arf Promoter During Eye Development. , Biotechniques 56 , 239-49 , 2014
Mouse Mouse, C57BL/6	Retinal astrocytes	Papain : 16.5 u/ml Deoxyribonuclease I : 0.004%	HBSS	Blandford, S. and Baldridge, W. The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i. , Biomed Res Int 2016 , 8178162 , 2016
Mouse Mouse, 4-5 week	Lens Fiber	Collagenase Type 4 : 0.125% Protease : 0.5%	PBS	Ebihara, L. , Tong, J. , Vertel, B. , White, T. and Chen, T. Properties of Connexin 46 Hemichannels in Dissociated Lens Fiber Cells. , Inv Ophthal Visual Sci 52 , 882-9 , 2011
Mouse Mouse, 2-4 week	Muller	PDS kit : per instructions	DMEM	Liu, X. , Tang, L. and Liu Y. Mouse Muller Cell Isolation and Culture. , Bio-protocol 7 , 2017
Mouse Mouse, adult	Stromal	Trypsin : 0.05% Collagenase : 78 u/ml Hyaluronidase : 38 u/ml	DMEM/F-12	Yoshida, S. , Shimmura, S. , Nagoshi, N. , Fukuda, K. , Matsuzaki, Y. , Okano, H. and Tsubota, K. Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea. , Stem Cells 24 , 2714-22 , 2006
Mouse Mouse, 2-5 month	Mouse retinal and bipolar	Papain : 20 u/ml Deoxyribonuclease I : 200 u/ml	HBSS	Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermuller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Kruger Olaf, Willecke Klaus, Weiler Reto. Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission , J Neurosci 25 , 566-76 , 2005
Mouse Mouse, 4-8 week	Corneal epithelial	Neutral Protease : 1.5% Trypsin : 0.25%	DMEM/F12	Kobayashi, T. , Yoshioka, R. , Shiraishi, A. and Ohashi, Y. New Technique for Culturing Corneal Epithelial Cells of Normal Mice. , Mol Vis 15 , 1589-93 , 2009
Mouse mouse, adult	Retinal	Papain : 50 u/ml	DMEM/F-12	Jadhav AP, Cho SH. and Cepko CL Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property , Proc Natl Acad Sci U S A 103 , 18998 , 2006
Mouse Mouse	Retinal	Papain : 20 u/ml Deoxyribonuclease I : 0.005%	PBS	Ortin-Martinez, A. , Tsai, E. , Nickerson, P. , Bergeret, M. , Lu, Y. , Smiley, S. , Comanita, L. and Wallace, V. A Reinterpretation of Cell Transplantation: GFP Transfer From Donor to Host Photoreceptors. , Stem Cells 35 , 932-939 , 2017
Mouse Mouse, 4 week	Retinal endothelial cells	Collagenase Type 1 : 0.1%	DMEM	Su X, Sorenson CM. and Sheibani N. Isolation and characterization of murine retinal endothelial cells , Mol Vis 9 , 171 , 2003
Mouse Mouse	Retinal	Papain : 20 u/ml Deoxyribonuclease I : 0.005%	HBSS	Wohl, S. , Jorstad, N. , Levine, E. and Reh, T. Muller Glial microRNAs are Required for the Maintenance of Glial Homeostasis and Retinal Architecture. , Nat Commun 8 , 1603 , 2017
Mouse Mouse, postnatal day 1	Retinal progenitor	Collagenase : 0.1%	HBSS	Jiang, C. Klassen, H. , Zhang, X. and Young, M. Laser Injury Promotes Migration and Integration of Retinal Progenitor Cells into Host Retina. , Mol Vis 16 , 983 , 2010
Mouse Mouse	Retinal ganglion	CLSPA : 240 u/ml Hyaluronidase : 0.2%	Ames	Schmidt, T. and Kofuji, P. An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells. , J Vis Exp 47 , 2367 , 2011
Mouse Mouse	Retinal	Collagenase : 0.1% Deoxyribonuclease I : 0.001%	RPMI	Amadi-Obi, A. , Yu, C. , Liu, X. , Mahdi, R. , Clarke, G. , Nussenblatt, R, Gery, I. , Lee, Y. and Egwuagu, C. TH17 Cells Contribute to Uveitis and Scleritis and are Expanded by IL-2 and Inhibited by IL-27/STAT1. , Nat Med 13 , 711 , 2007
Mouse Mouse, adult	Cilary epithelial	PDS kit : per instructions	EBSS	Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J. Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. , Curr Protoc Stem Cell Biol Chapter 1 , Unit 1H.4 , 2011
Mouse Mouse, 6 week	Retinal	PDS kit : per instructions	DMEM	Singh, M. , Issa, P, Butler, R. , Martin, C. , Lipinski, D. , Sekaran, S. , Barnard, A. and Maclaren, R. Reversal of End-Stage Retinal Degeneration and Restoration of Visual Bunction by Photoreceptor Transplantation. , Proc Natl Acad Sci U S A , 2013
Mouse Mouse, adult	Retinal photoreceptor	PDS kit : with modifications	EBSS	Feodorova, Y. , Koch, M. , Bultman, S. , Michalakis, S. and Solovei, I. Quick and Reliable Method for Retina Dissociation and Separation of Rod Photoreceptor Perikarya from Adult Mice. , MethodsX 2 , 39 , 2015
Mouse Mouse, 4-8 day	Photoreceptors	PDS kit : per instructions	Neurobasal	Balmer, J. , Zulliger, R. , Roberti, S. and Enzmann, V. Retinal Cell Death Caused by Sodium Iodate Involves Multiple Caspase-Dependent and Caspase-Independent Cell-Death Pathways. , Int J Mol Sci 16 , 15086-103 , 2015
Mouse Mouse, embryonic and P0	Retinal	Papain : 8 u/ml Deoxyribonuclease I : 124 u/ml	DPBS	Dvoriantchikova, G. , Perea-Martinez, I. , Pappas, S. , Barry, A. , Danek, D. , Dvoriantchikova, X. , Pelaez, D. and Ivanov, D. Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina. , PLoS ONE 10 , e0131054 , 2015
Porcine Porcine, 5-60 kg	Retinal pigment epithelial cells	Collagenase : 2%	DMEM	Wiencke, A. , Kiilgaard, J. , Nicolini, J. , Bundgaard, M. , Ropke, C. and La Cour, M. Growth of Cultured Porcine Retinal Pigment Epithelial Cells , Acta Opthalmol Scand 81(2) , 170 , 2003
Porcine Porcine, adult	Muller glia	PDS kit : per instructions	DMEM	Xu, N. , Chen, Y. , Dean, K. , Lu, X. , Liu, X. , Wang, W. , Dean, D. , Kaplan, H. , Gao, L. , Dong, F. and Liu, Y. Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation. , Stem Cells 35 , 1579-1591 , 2017
Rabbit Rabbit, New Zealand, adult, male, albino, 2 kg	Epithelial	Trypsin : 0.25%	HBSS/DMEM	Johnson-Muller, B. and Gross, J. Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions , Proc Natl Acad Sci U S A 75 (9) , 4417 , 1978
Rabbit Rabbit, adult	Retinal neurons	Papain : 26 u/ml	DMEM	Brockway LM, Zhou Z-H, Bubien JK, Jovov B, Benos DJ, Keyser KT. Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits , Am J Physiol Cell Physiol 283 , C126-34 , 2002
Rabbit Rabbit, New Zealand	Corneal keratocytes	Trypsin : 0.25% Collagenase : 0.5%	PBS	Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C. Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes , Inv Ophthal Visual Sci 45 , 807-12 , 2004
Rabbit Rabbit	Corneal keratocytes	Collagenase : 0.2% Hyaluronidase : 0.05%	DMEM	Hao, M. , Flynn, K. , Nien-Shy, C. , Jester, B. , Winkler, M. , Brown, D. , La Schiazza, O. , Bille, J. and Jester, J. In Vivo Non Linear Optical (NLO) Imaging in Live Rabbit Eyes Using the Heidelbert Two-Photon Laser Opthalmoscope , Exp Eye Res 91 , 308 , 2010
Rabbit Rabbit, New Zealand, Adult	Corneal endothelial	Collagenase Type 2 : 0.2%	HBSS	Lai, J. , Cheng, H. and Ma, D. Investigation of Overrun-Processed Porous Hyaluronic Acid Carriers in Corneal Endothelial Tissue Engineering , PLoS ONE 10 , 1371 , 2015
Rat Rat, embryonic 17 day	Retinal	Trypsin : 0.6%	DMEM	Seigel, G. The Golden Age of Retinal Cell Culture , Mol Vis 5 , 4 , 1999
Rat Rat, SD, pups	Retina	Trypsin : 0.25%	Ham's F-12	Sarthy PV, Curtis BM. and Catterall WA. Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat , J Neurosci 3 (12) , 2532 , 1983
Rat Rat, SD, 1-2 day	Retinal ganglion	Papain : 0.5% Deoxyribonuclease I : 0.1%	MEM	Lian, W. , Hu, X. , Zhang, J. , Wu, Y. , Zhao, N. , Ma, H. , He, H. and Lu, Q. Fucoxanthin Protects Retinal Ganglion Cells and Promotes Parkin-mediated Mitophagy against Glutamate Excitotoxicity. , Neuroreport 34 , 385-394 , 2023
Rat Rat, Long Evans, 1-15 days	Neurons, visual cortex	Papain : 20 u/ml	BSS (see reference)	Huettner, J. and Baughman, R. Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats , J Neurosci 6 , 3044 , 1986
Rat Rat, 4-14 week	Retinal pigment epithelial cells	Collagenase Type 1 : 65 u/ml Hyaluronidase : 220 u/ml	CF Hanks with EDTA	Wang N, Koutz CA. and Anderson RE. A method for the isolation of retinal pigment epithelial cells from adult rats , Inv Ophthal Visual Sci 34(1) , 101 , 1993
Rat Rat, 6-8 day	Retinal pigment epithelial	Neutral Protease : 2%	DMEM	Chang CW, Roque RS, Defoe DM. and Caldwell RB. An improved method for isolation and culture of pigment epithelial cells from rat retina , Curr Eye Res 10(11) , 1081 , 1991
Rat Rat, Dark Agouti, 3-4 week	Ciliary-derived eye	PDS kit : with modifications	DMEM/F12	Akagi T, Mandai M, Ooto S, Hirami Y, Osakada F, Kageyama R, Yoshimura N, Takahashi M. Otx2 homeobox gene induces photoreceptor-specific phenotypes in cells derived from adult iris and ciliary tissue , Inv Ophthal Visual Sci 45 , 4570 , 2004
Rat Rat, Fisher, adult	Retinal	PDS kit : per instructions	MEM	Suzuki, T. , Mandai, M. , Akimoto, M. , Yoshimura, N. and Takahashi, M. The Simultaneous Treatment of MMP-2 Stimulants in Retinal Transplantation Enhances Grafted Cell Migration into the Host Retina. , Stem Cells 24 , 2406 , 2006
Rat Rat, SD, 180-220g	Retinal ganglion	Papain : 0.2%	Neurobasal	Ma, J. , Yu, W. , Wang, Y. , Cao, G. , Cai, S. , Chen, X. , Yan, N. , Yuan, Y. , Zeng, H. , Fleenor, D. , Liu, X. and Pang, I. Neuroprotective Effects of C-type Natriuretic Peptide on Rat Retinal Ganglion Cells. , Inv Ophthal Visual Sci 51 , 3544-53 , 2010
Rat Rat, SD	Retinal ganglion	PDS kit : with modifications	Neurobasal	Tan, C. , Andrews, M. , Kwok, J. , Heintz, T. , Gumy, L. , Fassler, R. and Fawcett, J. Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration. , J Neurosci 32 , 7325 , 2012
Rat Rat, SD, 6-8 week	Retinal	PDS kit : per instructions	EBSS	Vigneswara, V. , Esmaeili, M. , Deer, L. , Berry, M. , Logan, A. and Ahmed, Z. Eye Drop Delivery of Pigment Epithelium-Derived Factor-34 Promotes Retinal Ganglion Cell Neuroprotection and Axon Regeneration. , Mol Cell Neurosci 68 , 212-21 , 2015
Rat Rat, Wister, 3-30 wk	Retinal	Papain : 30 u/ml Deoxyribonuclease I : 0.005%	EBSS	Cameron, M. , Kekesi, O. , Morley, J. , Tapson, J. , Breen, P. , Van Schaik, A. and Buskila, Y. Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue. , PLoS ONE 11 , e0155468 , 2016
Rat Adult, female, 170-200 g	Retinal	PDS kit : with modifications	EBSS	Mead, B. and Tomarev, S. Bone Marrow-Derived Mesenchymal Stem Cells-Derived Exosomes Promote Survival of Retinal Ganglion Cells Through miRNA-Dependent Mechanisms. , Stem Cells Transl Med 6 , 1273-1285 , 2017
Rat Rat, SD	Retina	Papain : 120 U	HBSS, PBS	Jing, S. , Wen, D. , Yu, Y. , Holst, P. , Luo, Y. , Fang, M. , Tamir, R. , Antonio, L. , Hu, Z. and et al. GDNF-Induced Activation of the Ret Protein Tyrosine Kinase is Mediated by GDNFR-a, a Novel Receptor for GDNF , Cell 85 , 1113 , 1996
Salamander Salamander, 18-25 cm	Retina	Papain : 14 u/ml	Saline	Townes-Anderson, E. , MacLeish, P. and Raviola, E. Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase , J Cell Biol 100 , 175 , 1985
Salamander Salamander (A. tigrinum)	Photoreceptors, retina	Papain : 0.05%	(see reference)	Bader, C. , MacLeish, P. and Schwartz, E. Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina , Proc Natl Acad Sci U S A 75 , 3507 , 1978
Salamander Salamander	Neurons	Papain : 14 u/ml	Ringers	Clarke, R, Wang, J. and Townes-Anderson, E. Using Laser Tweezers For Manipulating Isolated Neurons In Vitro. , J Vis Exp 19 , 911 , 2008
Turtle Turtle (Pseudemys scripta elegans)	Retinal	Papain : 0.1% (13.5 u/mg)	Kreb's Ringer	Lam, D. Biosynthesis of Acetylcholine in Turtle Photoreceptors , Proc Natl Acad Sci U S A 69 , 1987 , 1972