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Liver Tissue Tables

Species Cells Enzyme(s) Medium Reference
Avian
Ducklings, 7 day 		Hepatocytes DMEM/ F12
Lee J. , Culvenor J. , Angus P. , Smallwood R. , Nicoll A. and Locarnini S.
Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells ,
J Virol
 75(16) , 7651 , 2001
Avian
Avian, various 		Hepatocytes see reference
Else, P. , Brand, M. , Turner, N. and Hulbert, A
Respiration Rate of Hepatocytes Varies With Body Mass in Birds ,
J Exp Biol
 207 , 2305 , 2004
Bovine

Bovine, Holstein, 22-23 month

 Hepatocytes DMEM
Testroet, E. , Choudhary, S. , Choudhary, R. , Beitz, D. and Du, M.
Tumor Necrosis Factor Alpha and Palmitate Simulate Bovine Fatty Liver Disease in vitro when Using Abattoir-Derived Primary Bovine Hepatocytes Isolated by a Novel Nonperfusion Method. , JDS Commun Vol. 3 , 456-461 , 2022
Canine
Dog, 13 years old
(also rat, guinea-pig, rabbit, human) 		Hepatocytes CF EGTA perfusate
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Canine
Dog, adult 		Hepatocytes HBSS, CMF
Vickrey, H. , Ramon, J. and McCann, D.
Continuous Culture of Normal Adult Mammalian Hepatocytes Exhibiting Parenchymal Functions ,
In Vitro
 15 , 120 , 1979
Chicken
Chick embryonic 		Various tissues (heart, liver, skeletal, cardiac) CMF Tyrode's solution
Rinaldini, L.
An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells ,
Exp Cell Res
 16 , 477 , 1959
Chicken
Chick embryos, 5 day 		Heart
Liver 		Tyrode's solution, CMF
Steinberg, M.
"ECM": Its Nature, Origin, And Function in Cell Aggregation ,
Exp Cell Res
 30 , 257 , 1963
Chicken
Chicken, adult 		Hepatocytes HEPES, CF
Fraslin, J. , Touquette, L. , Douaire, M. , Menezo, Y. , Guillemot, J. and Mallard, J.
Isolation and Long Term Maintenance of Differentiated Adult Chicken Hepatocytes in Primary Culture ,
In Vitro Cell Dev Biol
 28 , 615 , 1992
Chicken
Chicken
(also rat, SD, 150-250 g) 		Hepatocytes HEPES
Weigel, P. , Schnaar, R. , Kuhlenschmidt, M. , Schmell, E. , Lee, R. , Lee, Y. and Roseman, S.
Adhesion of Hepatocytes to Immobilized Sugars ,
J Biol Chem
 254 (21) , 10830 , 1979
Chicken
Chicken, Leghorn, white, 10-15 day 		Hepatocytes PBS
Tarlow, D. , Watkins, P. , Reed, R. , Miller, R. , Zwergel, E. and Lane, M.
Lipogenesis and the Synthesis and Secretion of Very Low Density Lipoprotein by Avian Liver Cells in Nonproliferating Monolayer Culture ,
J Cell Biol
 73 , 332 , 1974
Equine
Equine, 4-13 yr 		Hepatocytes HBSS
Bakala A. , Karlik W. and Wiechetek M.
Preparation of Equine Isolated Hepatocytes ,
Toxicol In Vitro
 17(5-6) , 615 , 2003
Fish
Rainbow trout, (Salmo gairdneri), male, 150-200 g 		Hepatocytes HBSS/CMF
Klaunig, J. , Ruch, R. and Goldblah, P.
Trout Hepatocyte Culture: Isolation and Primary Culture ,
In Vitro Cell Dev Biol
 21 , 221 , 1985
Fish
Trout, male, 100 - 200 g 		Hepatocytes HBSS with 0.05M HEPES
Lipsky, M. , Sheridan, T. , Bennett, R. and May, E.
Comparison of Trout Hepatocyte Culture on Different Substrates ,
In Vitro Cell Dev Biol
 22 , 360 , 1986
Fish
Rainbow trout (Oncorhynchus mykiss), male & female,
120-600 g 		Hepatocytes Kreb's-Ringer bicarbonate buffer, CF
Blair, J.B. , Miller, M.R. , Pack, D. , Barnes, R. , Teh, S.J. and Hinton, D.E.
Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions ,
In Vitro Cell Dev Biol
 26 , 237 , 1990
Fish
Trout (Salmo gairdneri), male, 150-200 g
Catfish (Ictalurus punctatus), male, 100 - 150 g 		Hepatocytes HBSS
Klaunig, J.
Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies ,
Natl Cancer Inst Monogr
 65 , 163 , 1981
Fish
Trout 100g - 5kg 		Hepatocytes See reference
Bailey, G. , Taylor, M. and Selivonchick, D.
Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner ,
Carcinogenesis
 3 , 511 , 1982
Guinea-Pig
Guinea-pig, Hartley, male, 250-300 g 		Hepatocytes Kreb's Ringer bicarbonate buffer
Arinze, I. and Kawai, Y.
Adrenergic Regulation of Glycogenolysis in Isolated Guinea-Pig Hepatocytes: Evidence that B2-Receptors Mediate Catecholamine Stimulation of Glycogenolysis ,
Arch Biochem Biophys
 225 , 196 , 1983
Guinea-Pig
Guinea-pig, young 		Hepatocytes CF EGTA perfusate
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Human
Human 		Hepatocytes HEPES
Pichard L, Raulet E, Fabre G, Ferrini JB, Ourlin JC. and Maurel P
Human hepatocyte culture ,
Methods Mol Biol
 320 , 283 , 2006
Human
Human 		Hepatocytes EBSS
Hughes, R. , Mitry, R. , Dhawan, A. , Lehec, S. , Girlanda, R. , Rela, M. , Heaton, N. and Muiesan, P.
Isolation of Hepatocytes from Livers from Non-Heart-Beating Donors for Cell Transplantation ,
Liver Transpl
 12 , 713 , 2006
Human
Human 		Hepatocytes Williams E
Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O.
CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers ,
Drug Metab Dispos
 28 , 1321-6 , 2000
Human
Human 		Hepatocytes Williams E
Parzefall, W. , Erber, E. , Sedivy, R. and Schulte-Hermann, R.
Testing for Induction of DNA Synthesis in Human Hepatocyte Primary Cultures by Rat Liver Tumor Promoters ,
Cancer Res
 51 , 1143 , 1991
Human
Human, fetal 		Epithelial progenitor DMEM
Malhi, H. , Irani, A. , Gagandeep, S. and Gupta, S.
Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes. ,
J Cell Sci
 115 , 2679 , 2002
Human
Human 		Hepatic stem cells and heptoblasts various
Wauthier, E. , Schmelzer, E. , Turner, W. , Zhang, L. , LeCluyse, E. , Ruiz, J. , Turner, R. , Furth, M. , Kubota, H. , Lozoya, O. , Barbier, C. , McClelland, R. , Yao, H. , Moss, N. , Bruce, A. , Ludlow, J. and Reid, L.
Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance. ,
Methods Cell Biol
 86 , 137 , 2008
Human
Human 		Hepatic side population HBSS
Hussain, S. , Strom, S. , Kirby, M. , Burns, S. , Langemeijer, S. , Ueda, T. , Hsieh, M. and Tisdale, J.
Side Population Cells Derived from Adult Human Liver Generate Hepatocyte-Like Cells In Vitro. ,
Dig Dis Sci
 50 , 1755 , 2005
Human
Human, male, 17-40 yrs. 		Hepatocytes HEPES buffer
Begue, J. , Baffet, G. , Campion, J. and Guillouzo, A.
Differential Response of Primary Cultures of Human and Rat Hepatocytes to Aflatoxin B1-Induced Cytotoxicity and Protection by the Hepatoprotective agent(+)-Cyanidanol-3 ,
Biol Cell
 63 , 327 , 1988
Human
Human 		Hepatocytes Williams E
Torok, E. , Lutgehetmann, M. , Bierwolf, J. , Melbeck, S. , Dullmann, J. , Nashan, B. , Ma, P. and Pollok, J.
Primary Human Hepatocytes on Biodegradable Poly(l-lactic acid) Matrices: A Promising Model for Improving Transplantation Efficiency with Tissue Engineering. ,
Liver Transpl
 17 , 104 , 2011
Human
Human, 51-75 years old
(also rat, guinea-pig, rabbit, dog) 		Hepatocytes CF EGTA perfusate
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Human
Human, fetal 		Liver hematopoietic RPMI
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Human
Human 		Hepatocytes HEPES buffer
(see reference)
Gomez-Lechon, M. , Lopez, P. , Donato, T. , Montoya, A. , Larrauri, A. , Gimenez, P. , Trullenque, R. , Fabra, R. and Castell, J.
Culture of Human Hepatocytes From Small Surgical Liver Biopsies: Biochemical Characterization And Comparison With in vivo ,
In Vitro Cell Dev Biol
 26 , 67 , 1990
Human
Human 		Hepatocytes DMEM
Werner, M. , Driftmann, S. , Kleinehr, K. , Kaiser, G. , Mathe, Z. , Treckmann, J. , Paul, A. , Skibbe, K. , Timm, J. , Canbay, A. , Gerken, G. , Schlaak, J. and Broering, R.
All-In-One: Advanced Preparation of Human Parenchymal and Non-Parenchymal Liver Cells. ,
PLoS ONE
 10 , e0138655 , 2015
Human
Human 		Hepatocytes HBSS, CMF
Kaighn, M.
Human Liver Cells , Tissue Culture Methods / Applications , (Kruse, P., Patterson, M. eds) , 54 , 1973
Human
Human 		Hepatocytes HEPES
Lee, S. , Schelcher, C. , Demmel, M. , Hauner, M. and Thasler, W.
Isolation of Human Hepatocytes by a Two-Step Collagenase Perfusion Procedure. ,
J Vis Exp
 , 2013
Human
Human, 3 human kidney donors, age 20, 23, 25 		Hepatocytes HEPES
Gugen-Guillouzo, C. , Campion, J. , Brissot, P. , Glaise, D. , Launois, B. , Bourel, M. and Guillouzo, A.
High Yield Preparation of Isolated Human Adult Hepatocytes by Enzymatic Perfusion of the Liver ,
Cell Biol Int Rep
 6 (6) , 625 , 1982
Human
Human 		Hepatocytes and non-parenchymal cells HBSS
Pfeiffer, E. , Kegel, V. , Zeilinger, K. , Hengstler, J. , Nussler, A. , Seehofer, D. and Damm, G.
Featured Article: Isolation, Characterization, and Cultivation of Human Hepatocytes and Non-Parenchymal Liver Cells. ,
Exp Biol Med (Maywood)
 240 , 645-56 , 2015
Human
Human, 4 male kidney donors, age between 17 and 31
(also adult rats, male, SD, 180-200 g) 		Hepatocytes HEPES
(see reference)
LeBot, M. , Begue, J. , Kernaleguen, D. , Robert, J. , Ratanasavanh, D. , Airiau, J. , Riche, C. and Guillouzo, A.
Different Cytotoxicity and Metabolism of Doxorubicin, Daunorubicin, Epirubicin, Esorubicin, and Idarubicin in Cultured Human and Rat Hepatocytes ,
Biochem Pharmacol
 37 (20) , 3877 , 1988
Human
Human 		Hepatocytes HBSS
Reuschel, E, Jilg, W. , Seelbach-Goebel, B. and Demi, L.
Comparative Purification and Characterization of Hepatitis B Virus-like Particles Produced by Recombinant Vaccinia Viruses in Human Hepatoma Cells and Human Primary Hepatocytes ,
PLoS ONE
 , 2019
Human
Human
(also rat) 		Hepatocytes Leffert's buffer
Dandri, M. , Burda, M. , Torok, E. , Pollok, J. , Iwanska, A. , Sommer, G. and et al.
Repopulation of Mouse Liver with Human Hepatocytes and In Vivo Infection with Hepatitis B Virus ,
Hepatol
 33 , 981 , 2001
Human
Human 		Hepatocytes Williams E
Duanmu Z. , Locke D. , Smigelski J. , Dahn M. , Falany C. , Kocarek T. and Runge-Morris M.
Effects of Dexamethasone on Aryl (SULT1A1)- and hydroxysteroid (SULT2A1)-Sulfotransferase Gene Expression in Primary Cultured Human Hepatocytes ,
Drug Metab Dispos
 30(9) , 997 , 2002
Human
Human, adult 		Hepatocytes RPMI 1640
Cho JJ, Joseph B, Sappal BS, Giri RK, Wang R, Ludlow JW, Furth ME, Susick R. and Gupta S.
  Analysis of the functional integrity of cryopreserved human liver cells including xenografting in immunodeficient mice to address suitability for clinical applications ,
Liver Int
 24 , 361 , 2004
Monkey
Monkey (Macaca fascicularis), adult, 5-6 Kg 		Hepatocytes HEPES buffer
Ulrich, R. , Aspar, D. , Cramer, C. , Kletzien, R. and Ginsberg, L.
Isolation and Culture of Hepatocytes from the Cynogmolgus Monkey (Macaca fascicularis) ,
In Vitro Cell Dev Biol
 26 , 815 , 1990
Monkey
Monkey, Macaca mulatta, 3-5.5 kg 		Hepatocytes DMEM/F12
Weber, A. and Groyer-Picard, M.and Dagher, I.
Hepatocyte Transplantation Techniques: Large Animal Models. ,
Methods Mol Biol
 481 , 83 , 2009
Mouse
Mouse, C3H, 6-8 weeks 		Epithelial DMEM
Lillehaug, J. , Mondal, S. and Heidelberger, C.
Establishment of Epithelial Cell Lines from Mouse Regenerating Liver ,
In Vitro
 15 , 910 , 1979
Mouse
Mouse 		Hepatocytes & non-parenchymal liver cells DMEM
Brundert, M. , Heeren, J. , Merkel, M. , Carambia, A. , Herkel, J. , Groitl, P. , Dobner, T. , Ramakrishnan, R. , Moore, K. and Rinninger, F.
Scavenger Receptor CD36 Mediates Uptake of High Density Lipoproteins in Mice and by Cultured Cells. ,
J Lipid Res
 52 , 745 , 2011
Mouse
Mouse, 7 week 		Kupffer cells HBSS
Angele MK, Knoferl MW, Schwacha MG, Ayala A, Cioffi WG, Bland KI. and Chaudry IH.
Sex steroids regulate pro- and anti-inflammatory cytokine release by macrophages after trauma-hemorrhage ,
Am J Physiol
 277 , C35 , 1999
Mouse
Mouse 		Liver non-parenchymal HBSS
Bosschaerts, T. , Guilliams, M. , Stijlemans, B. , Morias, Y. , Engel, D. , Tacke, F. , Herin, M. , De Baetselier, P. and Beschin, A.
Tip-DC Development During Parasitic Infection is Regulated by IL-10 and Requires CCL2/CCR2, IFN-gamma and MyD88 Signaling. ,
PLoS Pathog
 6 , e1001045 , 2010
Mouse
Mouse 		Hepatocytes DMEM/ F-12
Sazani P. , Gemignani F. , Kang S. , Maier M. , Manoharan M. , Persmark M. , Bortner D. and Kole R.
Systemically Delivered Antisense Oligomers Up-regulate Gene Expression in Mouse Tissues ,
Nat Biotechnol
 20 , 1228 , 2002
Mouse
Mouse 		Hepatocytes DMEM
Kang, H. , Okamoto, K. , Kim, Y. , Takeda, Y. , Bortner, C. , Dang, H. , Wada, T. , Xie, W. , Yang, X. , Liao, G. and Jetten, A.
Nuclear Orphan Receptor TAK1/TR4-Deficient Mice are Protected Against Obesity-Linked Inflammation, Hepatic Steatosis, and Insulin Resistance. ,
Diabetes
 60 , 177 , 2011
Mouse
Mouse 		Liver epithelial progenitor cells DMEM
Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping.
Isolation and characterization of bipotent liver progenitor cells from adult mouse ,
Stem Cells
 24 , 322-32 , 2006
Mouse
Mouse 		Hepatacytes Williams E
Holl, D. , Kuckenberg, P. , Woynecki, T. , Egert, A. , Becker, A. , Huss, S. , Stabenow, D. , Zimmer, A. , Knolle, P. , Tolba, R. , Fischer, H. and Schorle, H.
Transgenic Overexpression of Tcfap2c/AP-2gamma Results in Liver Failure and Intestinal Dysplasia. ,
PLoS ONE
 6 , e22034 , 2011
Mouse
Mouse 		Nonparenchymal liver Hanks
Ling W, Lougheed M, Suzuki H, Buchan A, Kodama T, Steinbrecher UP.
Oxidized or acetylated low density lipoproteins are rapidly cleared by the liver in mice with disruption of the scavenger receptor class A type I/II gene ,
J Clin Invest
 100 , 244-52 , 1997
Mouse
Mouse 		CD133+ stem DMEM/F12
Rountree, C. , Ding, W. , Dang, H. , Vankirk, C. and Crooks, G.
Isolation of CD133+ Liver Stem Cells for Clonal Expansion. ,
J Vis Exp
 56 , e3183 , 2011
Mouse
Mouse 		Liver sinusodial endothelial DMEM
Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev.
Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice ,
Hepatol
 42 , 140-8 , 2005
Mouse
Mouse 		Hepatocytes and sinusoidal endothelial HBSS
Perepelyuk, M. , Terajima, M. , Wang, A. , Georges, P. , Janmey, P. , Yamauchi, M. and Wells, R.
Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. ,
Am J Physiol Gastrointest Liver Physiol
 304 , G605 , 2013
Mouse
Mouse, male, 4-10 week 		Hepatocytes HBSS
Lingohr Melissa K, Bull Richard J, Kato-Weinstein Junko, Thrall Brian D.
Dichloroacetate stimulates glycogen accumulation in primary hepatocytes through an insulin-independent mechanism ,
Toxicol Sci
 68 , 508-15 , 2002
Mouse
Mouse, 10-13 week 		Hepatocytes William's Medium E
Sewing, S. , Boess, F. , Moisan, A. , Bertinetti-Lapatki, C. , Minz, T. , Hedtjaern, M. , Tessier, Y. , Schuler, F. , Singer, T. and Roth, A.
Establishment of a Predictive In Vitro Assay for Assessment of the Hepatotoxic Potential of Oligonucleotide Drugs. ,
PLoS ONE
 11 , e0159431 , 2016
Mouse
Mouse 		Hepatocytes Leffert's buffer
Jiang Guoqiang, Li Zhihua, Liu Franklin, Ellsworth Kenneth, Dallas-Yang Qing, Wu Margaret, Ronan John, Esau Christine, Murphy Cain, Szalkowski Deborah, Bergeron Raynald, Doebber Thomas, Zhang Bei.
Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1 ,
J Clin Invest
 115 , 1030-8 , 2005
Mouse
Mouse 		Hepatocytes PBS
Weerasinghe, S. , Park, M. , Portney, D. and Omary, M.
Mouse Genetic Background Contributes to Hepatocyte Susceptibility to Fas-mediated Apoptosis. ,
Mol Biol Cell
 27 , 3005-3012 , 2016
Mouse
Mouse 		Hepatocytes Williams E
Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O.
CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers ,
Drug Metab Dispos
 28 , 1321-6 , 2000
Mouse
Mouse, female, 8-10 week 		Hepatocytes DMEM
Severgnini, M. , Sherman, J. , Sehgal, A. , Jayaprakash, N. , Aubin, J. , Wang, G. , Zhang, L. , Peng, C. , Yucius, K. , Butler, J. and Fitzgerald, K.
A Rapid Two-Step Method for Isolation of Functional Primary Mouse Hepatocytes: Cell Characterization and Asialoglycoprotein Receptor Based Assay Development. ,
Cytotechnology
 64 , 187-95 , 2012
Mouse
Mouse, male, 8 week 		Hepatocytes Waymouth's medium
Hatano E, Brenner DA.
Akt protects mouse hepatocytes from TNF-alpha- and Fas-mediated apoptosis through NK-kappa B activation. ,
Am J Physiol/Gastro
 282 , G1357 , 2002
Mouse
Mouse 		Hepatic HBSS
Bigorgne, A. , John, B. , Ebrahimkhani, M. , Shimizu-Albergine, M. , Campbell, J. and Crispe, I.
TLR4-Dependent Secretion by Hepatic Stellate Cells of the Neutrophil-Chemoattractant CXCL1 Mediates Liver Response to Gut Microbiota. ,
PLoS ONE
 11 , e0151063 , 2016
Mouse
Mouse, 8-10 week 		Liver derived stem cells HBSS
Kotton, D. , Fabian, A. and Mulligan, R.
A Novel Stem-Cell Population in Adult Liver with Potent Hematopoietic-Reconstitution Activity. ,
Blood
 106 , 1574 , 2005
Mouse
Mouse 		Hepatocytes HBSS
Shmarakov, I. , Fleshman, M. , D'Ambrosio, D. , Piantedosi, R. , Riedl, K. , Schwartz, S. , Curley, R. , von Lintig, J. , Rubin, L. , Harrison, E. and Blaner, W.
Hepatic Stellate Cells are an Important Cellular Site for β-Carotene Conversion to Retinoid. ,
Arch Biochem Biophys
 504 , 3-10 , 2010
Mouse
Mouse, 12 week 		Hepatic stellate DMEM
Mederacke, I. , Dapito, D. , Affo, S. , Uchinami, H. and Schwabe, R.
High-Yield and High-Purity Isolation of Hepatic Stellate Cells from Normal and Fibrotic Mouse Livers. ,
Nat Protoc
 10 , 305-15 , 2015
Mouse
Mouse, male 		Hepatocytes Williams E
Oliva, J. , Bardag-Gorce, F. , French, B. , Li, J. , McPhaul, L. , Amidi, F. , Dedes, J. , Habibi, A. , Nguyen, S. and French, S.
Fat10 is an Epigenetic Marker for Liver Preneoplasia in a Drug-Primed Mouse Model of Tumorigenesis. ,
Exp Mol Pathol
 84 , 102 , 2008
Mouse

7-10 week

 Hepatocytes Williams E
Plata-Gómez, A. , Crespo, M. , Arregui, C. , Prado-Rivas, L. , Sabio, G. and Efeyan, A.
Protocol for the Assessment of mTOR Activity in Mouse Primary Hepatocytes. , STAR Protoc Vol. 2 , 100918 , 2021
Mouse
Mouse 		hepatocytes DMEM
Sin, Y. , Ballantyne, L. , Mukherjee, K. , St Amand, T. , Kyriakopoulou, L. , Schulze, A. and Funk, C.
Inducible Arginase 1 Deficiency in Mice Leads to Hyperargininemia and Altered Amino Acid Metabolism. ,
PLoS ONE
 8 , e80001 , 2013
Mouse
Mouse, male, 12-14 week 		Hepatocytes Williams E
Chung, S. , Timmins, J. , Duong, M. , Degirolamo, C. , Rong, S. , Sawyer, J. , Singaraja, R. , Hayden, M. , Maeda, N. , Rudel, L. , Shelness, G. and Parks, J.
Targeted Deletion of Hepatocyte ABCA1 Leads to VLDL Triglyceride Over-Production and LDL Hypercatabolism ,
J Biol Chem
 285 , 12197 , 2010
Mouse
Mouse 		Hepatocytes DMEM
Guenthner, C. , Luitje, M. , Pyle, L. , Molyneux, P. , Yu, J. , Li, A. , Leise, T. and Harrington, M.
Circadian Rhythms of Per2::Luc in Individual Primary Mouse Hepatocytes and Cultures. ,
PLoS ONE
 9 , e87573 , 2014
Mouse
Mouse, male, adult 		Hepatocytes HBSS
Mathijs, K. , Kienhuis, A. , Brauers, K. J, Jennen, D. , Lahoz, A. , Kleinjans, J. and van Delft, J.
Assessing the Metabolic Competence of Sandwich-Cultured Mouse Primary Hepatocytes. ,
Drug Metab Dispos
 37 , 1305 , 2009
Mouse
Mouse 		Hepatocytes DMEM
Cabral, F. , Miller, C. , Kudrna, K. , Hass, B. , Daubendiek, J. , Kellar, B. and Harris, E.
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion. ,
J Vis Exp
 , 2018
Mouse
Mouse, 7-10 week 		Liver endothelial DMEM
Follenzi, A. , Benten, D. , Novikoff, P. , Faulkner, L. , Raut, S. and Gupta, S.
Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice. ,
J Clin Invest
 118 , 935 , 2008
Mouse
Mouse, 20-30 g 		Parenchymal and non-parenchymal Hank's w/ Insulin, CMF
Crisp, D. and Pogson, C.
Glycolytic and Gluconeogenic Enzyme Activities in Parenchymal and Non-parenchymal Cells from Mouse Liver ,
Biochem J
 126 , 1009 , 1972
Mouse
Mouse, 10-12 week 		Liver sinusoidal endothelial cells HEPES
Beldi, G. , Wu, Y. , Sun, X. , Imai, M. , Enjyoji, K. , Csizmadia, E. , Candinas, D. , Erb, L. and Robson, S.
Regulated Catalysis of Extracellular Nucleotides by Vascular CD39/ENTPD1 is Required for Liver Regeneration. ,
Gastroenterol
 135 , 1751 , 2008
Porcine
Porcine, one week 		Hepatocytes RPMI 1640
Wang Y. , Liu H. , Guo H. , Wen H. and Liu J.
Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich ,
World J Gastroenterol
 10 , 699 , 2004
Porcine
Porcine, adult 		Hepatocytes MEM
Zhou X, Liu L, Kano J, Mukaiyama T. and Tokiwa T.
Isolation and Cultivation of Porcine Hepatocytes for Extracorporeal Artificial Liver Support System ,
Chin Med J
 114 , 946 , 2001
Porcine
Porcine, male, 2-3 week 		Hepatocytes DMEM
Raman Priya, Donkin Shawn S, Spurlock Michael E.
Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures ,
Am J Physiol Regul Integr Comp Physiol
 286 , R206-16 , 2004
Porcine
Porcine, Chinese mini pig, 6-10 kg 		Hepatocytes Williams E
Li, J, Li, L. , Chao, H. , Yang, Q. , Liu, X. , Sheng, J. , Yu, H. and Huang, J
Isolation and Short Term Cultivation of Swine Hepatocytes for Bioartificial Liver Support System. ,
Hepatobiliary Pancreat Dis Int
 4 , 249 , 2005
Porcine
Porcine, mini-pig, 13 kg 		Hepatocytes Williams E
Meng, F. , Chen, Z. , Han, M. , Hu, X. , He, X. , Liu, Y. , He, W. , Huang, W. , Guo, H. and Zhou, P.
Porcine Hepatocyte Isolation and Reversible Immortalization Mediated by Retroviral Transfer and Site-Specific Recombination. ,
World J Gastroenterol
 16 , 1660 , 2010
Porcine
Porcine, Yorkshire, male, 21 day 		Hepatocytes Williams E
Terner, M. , Gilmore, W.J. , Lou, Y. and Squires, E.J.
The Role of CYP2A and CYP2E1 in the Metabolism of 3-Methylindole in Primary Cultured Porcine Hepatocytes. ,
Drug Metab Dispos
 34 , 848 , 2006
Porcine
Porcine, male, 6-40 kg 		Hepatocytes PBS
Gerlach, J. , Brombacher, J. , Smith, M. and Neuhaus, P.
High Yield Hepatocyte Isolation from Pig Livers for Investigation of Hybrid Liver Support Systems: Influence of Collagenase Concentration and Body Weight ,
J Surg Res
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Rabbit
Rabbit, New Zealand white 		Hepatocytes CF EGTA perfusate
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
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Rat
Rat 		Hepatocytes DMEM
Matsumoto, K. and Nakamura, T
Techniques for the Isolation and Cultivation of Hepatocytes using Collagenase , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:16.1 , 1995
Rat
Rat, Fisher, 10 day 		Epithelial-like PBS
Williams, G. , Weisburger, E. and Weisburger, J.
Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver ,
Exp Cell Res
 69 , 106 , 1971
Rat
Rat, Fisher, male, 125-250 g 		Hepatocytes RPMI 1640
Chan, K. , Kost, D. and Michalopoulos, G.
Multiple Sequential Periods of DNA Synthesis and Quiescence in Primary Hepatocyte Cultures Maintained on the DMSO-EGF On/Off Protocol ,
J Cell Physiol
 141 , 584 , 1989
Rat
Rat, SD, male/female, 250-300 g 		Hepatocytes Krebs Henseleit bicarbonate buffer
Studer, R. and Borle, A.
Differences between Male and Female Rats in the Regulation of Hepatic Glycogenolysis. The Relative Role of Calcium and cAMP in Phosphorylase Activation by Catecholamines ,
J Biol Chem
 257 , 7987 , 1982
Rat
Rat, adult 		Portal fibroblasts DMEM/F-12
Wen, J. , Olsen, A. , Perepelyuk, M. and Wells, R.
Isolation of Rat Portal Fibroblasts by In Situ Liver Perfusion. ,
J Vis Exp
 64 , e3669 , 2012
Rat
Rat, Fischer, male, 150-250 g 		Hepatocytes MEM
(see reference)
Francavilla, A. , Ove, P. , Polimeno, L. , Sciascia, C. , Coetzee, M. and Starzi, T.
Epidermal Growth Factor and Proliferation in Rat Hepatocytes in Primary Culture Isolation at Different Times after Partial Hepatectomy ,
Cancer Res
 46 , 1318 , 1986
Rat
Rat, SD, male, 175-225 g 		Hepatocytes Kreb's Ringer bicarbonate buffer
Reddy, S. , Amick, G. , Cooper, R. and Damun, Z.
Insulin Stimulates the Activity of a Protamine in Isolated Rat Hepatocytes ,
J Biol Chem
 265 , 7748 , 1990
Rat
Rat, Wistar, 200-250 g 		Hepatocytes HBSS, CF
Berg, T. , Boman, D. and Seglen, P.O.
Induction of Tryptophan Oxygenase in Primary Rat Liver Cell Suspensions by Glucocorticoid Hormone ,
Exp Cell Res
 72 , 571 , 1972
Rat
Rat, SD, male, 200-250 g 		Hepatocytes HBSS
Kreamer, B. , Staecker, J. , Sawada, N. , Sattler, G. , Hsia, M. and Pitot, H.
Use of Low-Speed, Iso-Density Percoll Centrifugation Method to Increase the Viability of Isolated Rat Hepatocyte Preparations ,
In Vitro Cell Dev Biol
 22 (4) , 201 , 1986
Rat
Rat, Wistar, male, 200 g 		Parenchymal HEPES, modified
Kindberg, G. , Gudmundsen, O. and Berg, T.
The Effect of Vandate on Receptor-mediated Endocytosis of Asialoorosomucoid in Rat Liver Parenchymal Cells ,
J Biol Chem
 265 , 8999 , 1990
Rat
Rat 		Hepatocytes DMEM
Budick-Harmelin, N. , Anavi, S. , Madar, Z. and Tirosh, O.
Fatty Acids-Stress Attenuates Gluconeogenesis Induction and Glucose Production in Primary Hepatocytes. ,
Lipids Health Dis
 11 , 66 , 2012
Rat
Rat, Fisher, adult, male 		Hepatocytes HBSS
(see reference)
Williams, G. , Bermudez, E. and Scaramuzzino, D.
Rat Hepatocytes Primary Cell Cultures III. Improved Dissociation and Attachment Techniques and the Enchancement of Survival by Culture Medium ,
In Vitro
 13 (12) , 809 , 1977
Rat
Rat, SD, male 		Hepatocytes Eagle's
Eagle's w/HEPES
HBSS
Oka, J. and Weigel, P.
Monensin Inhibits Ligand Dissociation Only Transiently and Partially and distinguishes two galactosyl receptor pathways in isolated rat hepatocytes ,
J Cell Physiol
 133 , 243 , 1987
Rat
Rat, Fischer, adult, 200-250 g 		Epithelial KCl-NaCl HEPES Buffer
Williams, G. and Gunn, J.
Long-Term Culture of Adult Rat Liver Epithelial Cells ,
Exp Cell Res
 89 , 139 , 1974
Rat
Rat, SD, male, 230-250g 		Hepatocytes HEPES
Doleh, L. and Romani, A.
Biphasic Effect of Extra-Reticular Mg2+ on Hepatic G6P Transport and Hydrolysis. ,
Arch Biochem Biophys
 467 , 283 , 2007
Rat
Rat, SD, adult, 200-300 g 		Parenchymal HBSS, CF
Berry, M. and Friend, D.
High Yield Preparation of Isolated Rat Liver Parenchymal Cells ,
J Cell Biol
 43 , 506 , 1969
Rat
Rat, Wistar, 270-330g 		Hepatocytes Williams E
Li, W. , Ralphs, K. , Slack, J. and Tosh, D.
Keratinocyte Serum-Free Medium Maintains Long-Term Liver Gene Expression and Function in Cultured Rat Hepatocytes by Preventing the Loss of Liver-Enriched Transcription Factors. ,
Int J Biochem Cell Biol
 39 , 541-54 , 2007
Rat
Rat, 250-350 g
(also guinea-pig & rabbit) 		Hepatocytes DMEM
Chang, T. and Chang, C.
Hepatic Uptake of Asialoglycoprotein is Different Among Mammalian Species Due to Different Receptor Distribution ,
Biochim Biophys Acta
 942 , 57 , 1988
Rat
Rat, SD 		Hepatocytes CF EGTA perfusate
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Rat
Rat, Wistar, male, 200-250 g 		Hepatocytes Saline
Poli, G. , Gravela, E. , Albano, E. and Dianzani, M.
Studies on Fatty Liver with Isolated Hepatocytes: II., The Action of Carbon Tetrachloride on Lipid Peroxidation, Protein and Triglyceride Synthesis and Secretion ,
Exp Mol Pathol
 30 , 116 , 1979
Rat
Rat, 200-300 g 		Hepatocytes HBSS
Mula, N. , Cubero, F. , Codesal, J. , de Andres, S. , Escudero, C. , Garcia-Barrutia, S. , Millan, I. , Arahuetes, R. and Maganto, P.
Survival of Allogeneic Hepatocytes Transplanted into the Thymus. ,
Cells Tissues Organs
 188 , 270 , 2008
Rat
Rat, Fisher 344, pregnant (19-21 days gestation),
200-250 g 		Hepatocytes Modified Eagle's w/ Serum
Leffert, H. and Paul, D.
Studies on Primary Cultures of Differentiated Fetal Liver Cells ,
J Cell Biol
 52 , 559 , 1972
Rat
Rat, SD, male 		Hepatocytes Williams E
Burczynski, M. , McMillian, M. , Parker, J. , Bryant, S. , Leone, A. , Grant, E. , Thorne, J. , Zhong, Z. , Zivin, R. and Johnson, M.
Cytochrome P450 Induction in Rat Hepatocytes Assessed by Quantitative Real-Time Reverse-Transcription Polymerase Chain Reaction and the RNA Invasive Cleavage Assay. ,
Drug Metab Dispos
 29 , 1243 , 2001
Rat
Rat, SD, male, 180-200 g 		Hepatocytes MEM
Gupta, C. , Hattori, A. , Betschart, J. , Virji, M. and Shinozuka, H.
Modulation of Epidermal Growth Factor Receptors in Rat Hepatocytes by Two Liver-Promoting Regimens, a Choline-Deficient and a Phenobarbital Diet ,
Cancer Res
 48 , 1162 , 1988
Rat
Rat, SD, 7-10 day 		Liver HBSS modified (see reference)
Acosta, D. , Anuforo, D. and Smith, R
Preparation of Primary Monolayer Cultures of Postnatal Rat Liver Cells ,
J Tiss Cul Meth
 6 , 35 , 1980
Rat
Rat, SD, male, 270 - 320 g 		Hepatocytes Bicarbonate buffer with calcium added
Brass, E. , Garrity, M. and Robertson, R.
Inhibition of Glucagon-Stimulated Hepatic Glycogenolysis by E-Series Prostaglandins ,
F.E.B.S. Lett.
 169 , 293 , 1984
Rat
Rat, Lewis, 150-200g 		Hepatocytes Williams E
Smith, M. and Mooney, D.
Hypoxia Leads to Necrotic Hepatocyte Death. ,
J Biomed Mater Res A
 80 , 520 , 2007
Rat
Rat, SD, adult, male, 200-250 g 		Parenchymal HBSS, CF
Howard, R. , Lee, J. and Pesch, L.
The fine structure, potassium content, and respiratory activity of isolated rat liver parenchymal cells prepared by improved enzymatic techniques ,
J Cell Biol
 57 , 642 , 1973
Rat
Rat, SD, 500-700g 		Hepatocytes and sinusoidal endothelial HBSS
Perepelyuk, M. , Terajima, M. , Wang, A. , Georges, P. , Janmey, P. , Yamauchi, M. and Wells, R.
Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. ,
Am J Physiol Gastrointest Liver Physiol
 304 , G605 , 2013
Rat
Rat, Wistar, male, 260-310 g 		Hepatocytes HEPES
Seglen, P.
Preparation of Rat Liver Cells ,
Exp Cell Res
 74 , 450 , 1972
Rat
Rat, adult 		Hepatocytes Williams E
Jensen C. , Jauho E. , Santoni-Rugiu E. , Holmskov U. , Teisner B. , Tygstrup N. and Bisgaard H.
Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1 ,
Am J Pathol
 164(4) , 1347 , 2004
Rat
Rat, SD, 8 - 12 day 		Parenchymal hepatocytes HBSS
Davila, J. , Reddy, C. , Davis, P. and Acosta, D.
Toxicity Assessment of Papaverine Hydrochloride and Papaverine-Derived Metabolites in Primary Cultures of Rat Hepatocytes ,
In Vitro Cell Dev Biol
 26 , 515 , 1990
Rat
Rat, Wistar, female 		Hepatic stellate cells DMEM
Zvibel, I. , Atias, D. , Phillips, A. , Halpern, Z. and Oren, R.
Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho. ,
Lab Invest
 90 , 674 , 2010
Rat
Rat, SD, adult, male, 180-300 g 		Parenchymal Hank's solution, CF
Bissell, D. , Hammaker, L. and Meyer, U.
Parenchymal Cells from Adult Rat Liver in Nonproliferating Monolayer Culture. I. Functional Studies ,
J Cell Biol
 59 , 722 , 1973
Rat
Rat, SD, 500-750 g 		Portal fibroblasts DMEM/F-12
Li, Z. , Dranoff, J. , Chan, E. , Uemura, M. and Sevigny, J.and Wells, R.
Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture. ,
Hepatol
 46 , 1246-56 , 2007
Rat
Rat, Wistar, male, 180-210 g 		Hepatocytes Kreb's buffer
De Gerlache, J. , Lans, M. , Taper, H. and Roberfroid, M.
Separate Isolation of Cells from Nodules and Surrounding Parenchyma of the Same Precancerous Rat Liver: Biochemical and Cytochemical Characterization ,
Toxicology
 18 , 225 , 1980
Rat
Rat, male, 3 week 		Hepatocytes Williams E
Low-Baselli A. , Hufnagl K. , Parzefall W. , Schulte-Hermann R. and Grasl-Kraupp B.
Initiated Rat Hepatocytes in Primary Culture: A Novel Tool to Study Alterations in Growth Control During the First Stage of Carcinogenesis ,
Carcinogenesis
 21 , 79 , 2000
Rat
Rat, SD, male, 250-350 g 		Hepatocytes HBSS, CMF
Liu, J. , Kershaw, W. and Klaassen, C.
Rat Primary Hepatocyte Cultures are a Good Model for Examining Metallothionein-Induced Tolerance to Cadmium Toxicity ,
In Vitro Cell Dev Biol
 26 , 75 , 1990
Rat
Rat, SD, 180-200g 		Hepatocytes see reference
Charbonneau, A. , Unson, C. and Lavoie, J.
High-Fat Diet-Induced Hepatic Steatosis Reduces Glucagon Receptor Content in Rat Hepatocytes: Potential Interaction with Acute Exercise. ,
J Physiol
 579 , 255 , 2007
Rat
Rat, Wistar, male, 6-8 weeks, 80-160 G 		Hepatocytes HBSS, CMF
Iype, P.
Cultures from Adult Rat Liver Cells. 1.Establishment of Monolayer Cell-Cultures from Normal Lver ,
J Cell Physiol
 78 , 281 , 1971
Rat
Rat, Fisher 344, 150-200 g 		Bile ductular epithelial L-15
Mathis, G. , Walls, S. and Sirica, A.
Biochemical Characteristics of Hyperplastic Rat Bile Ductular Epithelial Cells Cultured "on Top" and "Inside" Different Extracellular Matrix Substitutes ,
Cancer Res
 48 , 6145 , 1988
Rat
Rat, SD, male, 350 - 450 g 		Lipocytes
Kupffer
Sinusoidal endothelial 		DMEM/Ham's F-12
Friedman, S. and Roll, F.
Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan ,
Anal Biochem
 161 , 207 , 1987
Rat
Rat, Lewis, female, 150-200 g 		Hepatocytes DMEM
Sosef MN, Baust JM, Sugimachi K, Fowler A, Tompkins RG. and Toner M.
Cryopreservation of isolated primary rat hepatocytes: enhanced survival and long-term hepatospecific function ,
Ann Surg
 241 , 125 , 2005
Rat
Rat, Wistar, adult, male, 100-125 g 		Hepatocytes Serum-free medium
Liu, J. , McKim, J. , Liu, Y. and Klaassen, C.
Effects of Butyrate Homologes on Metallothionein Induction in Rat Primary Hepatocyte Cultures ,
In Vitro Cell Dev Biol
 28 , 320 , 1992
Rat
Rat, Wistar 		Hepatocytes RPMI 1640
Wang Y. , Liu H. , Guo H. , Wen H. and Liu J.
Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich ,
World J Gastroenterol
 10 , 699 , 2004
Rat
Rat (WAG/RIJ), female, 24, 30, and 37 months 		Parenchymal HEPES buffer
VanBezodijen, C. , Grell, T. and Knook, D.
Effect of Age on Protein Synthesis by Isolated Liver Parenchymal Cells ,
Mech Ageing Dev
 6 , 293 , 1977
Rat
Rat, Wistar, male, 3 month old 		Endothelial
Kupffer
Parenchymal 		HBSS
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Rat
Rat, 100-200 g 		Hepatocytes HBSS, CF
Haung, Y. and Ebner, K.
Induction of Tyrosine Aminotransferase in Isolated Liver Cells ,
Biochim Biophys Acta
 191 , 161 , 1969
Rat
Rat, Lewis, adult 		Hepatocytes HEPES
Matsuura T, Gad MZ, Harrison EH. and Ross AC
Lecithin:retinol acyltransferase and retinyl ester hydrolase activities are differentially regulated by retinoids and have distinct distributions between hepatocyte and nonparenchymal cell fractions of rat liver ,
J Nutr
 127 , 218 , 1997
Rat
Rat, Fischer, male, adult, 170-265 g 		Hepatocytes Williams E
Laishes, B. and Williams, G.
Conditions Affecting Primary Cell Cultures of Functional Adult Rat Hepatocytes. I.. The Effect of Insulin ,
In Vitro
 12 , 521 , 1976
Rat
Rat, SD 		Hepatocytes HBSS
Chung, C. , Shugrue, C. , Nagar, A. , Doll, J. , Cornwell, M. , Gattu, A. , Kolodecik, T. , Pandol, S. and Gorelick, F.
Ethanol Exposure Depletes Hepatic Pigment Epithelium-Derived Factor, a Novel Lipid Regulator. ,
Gastroenterol
 136 , 331 , 2009
Rat
Rat, embryos, 1-3 days 		Hepatocytes HBSS
Gerschenson, L. , Berliner, J. and Davidson, M.
The Isolation and Culture of Liver Cells , Vol. 32 , 733 , 1974
Rat
Rat
(also chicken) 		Hepatocytes HEPES
Weigel, P. , Schnaar, R. , Kuhlenschmidt, M. , Schmell, E. , Lee, R. , Lee, Y. and Roseman, S.
Adhesion of Hepatocytes to Immobilized Sugars ,
J Biol Chem
 254 (21) , 10830 , 1979
Rat
Rat, Fischer 344, male, 14 months 		Hepatocytes Ringer's biocarbonate buffer
Nagy, I. , Ohno-Iwashita, Y. , Ohta, M. , Nagy, V. , Kitani, K. , Ando, S. and Imahori, K.
Effect of Perfringolysin O on the Lateral Diffusion Constant of Membrane Proteins of Hepatocytes as Revealed by Fluorescence Recovery After Photobleaching ,
Biochim Biophys Acta
 939 , 551 , 1988
Rat
Rat 		Liver sinusodial endothelial DMEM
Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev.
Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice ,
Hepatol
 42 , 140-8 , 2005
Rat
Rat, Wistar, female, fetus 		Hepatocytes HEPES buffer
Gugen-Guillouzo, C. , Tichonicky, L. , Szajnert, M. and Kruh, J.
Changes in Some Chromatin and Cytoplastic Enzymes of Perinatal Rat Hepatocytes ,
In Vitro
 16 , 1 , 1980
Rat
Rat, SD 		Hepatic stellate cells Medium 199
Handy, J. , Saxena, N. , Fu, P. , Lin, S. , Mells, J. , Gupta, N. and Anania, F.
Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3). ,
J Cell Biochem
 110 , 1195 , 2010
Rat
Rat, SD, female, 100-180 g 		Hepatocytes Krebs Ringer bicarbonate buffer
Schwarz, K. , Lanier, S. , Carter, E. , Homcy, C. and Graham, R.
Rapid Reciprocal Changes in Adrenergic Receptors in Intact Isolated Hepatocytes During Primary Cell Culture ,
Mol Pharmacol
 27 , 200 , 1985
Rat

Rat, 8-14 week

 Parenchymal and non-parenchymal Williams E
Ge, J. , Zheng, Y. , Tsuchida, T. , Furuya, K. , Isoda, H. , Taniguchi, H. , Ohkohchi, N. and Oda, T.
Hepatic Stellate Cells Contribute to Liver Regeneration through Galectins in Hepatic Stem Cell Nliche. ,
Stem Cell Res Ther
 11 , 425 , 2020
Rat
Rat, SD, male, 450-500 g 		Stellate
  • Protease : 0.02%
HBSS
Gabriel, A. , Kuddus, R. , Rao, A. and Gandhi, C.
Down-Regulation of Endothelin Receptors by Transforming Growth Factor B1 in Hepatic Stellate Cells ,
J Hepatol
 30 , 440 , 1999
Rat
Rat, SD, male, 230-250 g 		Hepatocytes HBSS
Kuddus, R. , Nalesnik, M. , Subbotin, V. , Rao, A. and Gandhi, C.
Enhanced Sythesis and Reduced Metabolism of Endothelin-1 (ET-1) by Hepatocytes - An Important Mechanism of Increased Endogenous Levels of ET-1 in Liver Cirrhosis ,
J Hepatol
 33 , 725 , 2000
Rat
Rat, SD, male, 200-300 g 		Hepatocytes HEPES
Voss, A. and Sprecher, H.
Metabolism of 6,9,12-Octadecatetraenoic and 6,9,12,15-Octadecatetraenoic Acid ,
Biochim Biophys Acta
 958 , 153 , 1988
Rat
Rat, Lewis, female, 200-250 g 		Hepatocytes, Kupffer, endothelial Gey's BSS
Holstege A, Leser HG, Pausch J, Gerok W.
Uridine catabolism in Kupffer cells, endothelial cells, and hepatocytes ,
Eur J Biochem
 149 , 169-73 , 1985
Rat
Rat, SD, male, 250-300 g 		Parenchymal Kreb's Henseleit bicarbonate buffer
Yamada, S. , Otto, P. , Kennedy, D. and Whayne, T.
The Effects of Dexamethasone on Metabolic Activity of Hepatocytes in Primary Monolayer Culture ,
In Vitro
 16 , 559 , 1980
Rat
Rat, Lewis, male 		Hepatocytes DPBS
Bettinger, C. , Kulig, K. , Vacanti, J. , Langer, R. and Borenstein, J.
Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture. ,
Tissue Eng Part A
 15 , 1321 , 2009
Rat
Rat, CD strain, albino, male, 140 -180 g 		Hepatocytes and Nonparenchymal
  • Pronase : 0.1%
HEPES buffer with calcium
Braun, L. , Mead, J. , Panzica, M. , Mikumo, R. , Bell, G. and Fausto, N.
Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation ,
Proc Natl Acad Sci U S A
 85 , 1539 , 1988
Rat

Wistar, male, 200-250 g

 Hepatocytes DMEM
Berardo, C. , Ferrigno, A. , Siciliano, V. , Richelmi, P. , Vairetti, M. and Pasqua, L.
Isolation of Rat Hepatocytes for Pharmacological Studies on Metabotropic Glutamate Receptor (mGluR) Subtype 5: A Comparison Between Collagenase I Versus Collagenase IV. ,
Eur J Histochem
 64 , 2020
Rat
Rat, Wistar, male, 260-310 g 		Hepatocytes HEPES
McAbee DD. and Weigel PH
ATP-dependent inactivation and reactivation of constitutively recycling galactosyl receptors in isolated rat hepatocytes ,
Biochemistry
 27 , 2061 , 1988
Rat
Rat, Wistar, male,3 month, 200 g 		Parenchymal
Endothelial
Kupffer 		Krebs Henseleit
Kuiper, J. , Zijlstra, F. , Kamps, J. and vanBerkel, T.
Identification of Prostaglandin D2 as the Major Eiconsanoid from Liver Endothelial and Kupffer Cells ,
Biochim Biophys Acta
 959 , 143 , 1988
Rat
Rat, Wistar, 200-300 g 		Hepatocytes Williams E
Putz G, Schmider W, Nitschke R, Kurz G, Blum HE.
Synthesis of phospholipid-conjugated bile salts and interaction of bile salt-coated liposomes with cultured hepatocytes ,
J Lipid Res
 46 , 2325-38 , 2005
Rat
Rat, Zucker, obese 		Hepatocytes Dulbecco-Vogt arginine free Eagle's
Goldstein, A. , Palmer, J. and Johnson, P.
Primary Cultures of Fetal Hepatocytes from the Genetically Obese Zucker Rat: Protein Synthesis ,
In Vitro
 17 , 651 , 1981
Rat
Rat, SD, 240-320g 		Hepatocytes Waymouth's MB
Pillai, V. and Mehvar, R.
Inhibition of NADPH-Cytochrome P450 Reductase by Tannic Acid in Rat Liver Microsomes and Primary Hepatocytes: Methodological Artifacts and Application to Ischemia-Reperfusion Injury ,
J Pharm Sci
 100 , 3495 , 2011
Rat
Rat, SD, neonatal, 8 - 10 days 		Hepatocytes Hanks' BSS, CF
Davila, J. , Lenherr, A. and Acosta, D.
Protective Effect of Flavonoids on Drug-Induced Hepatotoxicity In Vitro ,
Toxicology
 57 , 267 , 1989
Rat
Rat , Wistar, male, 250-300 g 		Hepatocytes Hank's solution, CF
Gravela, E. , Poli, G. , Albano, E. and Dianzani, M.
Studies of Fatty Liver with Isolated Hepatocytes ,
Exp Mol Pathol
 27 , 339 , 1977
Rat
Rat, Wistar, male, 200 - 300 g 		Parenchymal
Kupffer 		HBSS with CaCl2
Cai, H. , He, Z. and Ding, Y.
Effects of Monocyte Macrophages Stimulation on Hepatic Lipoprotein Receptors ,
Biochim Biophys Acta
 958 , 334 , 1988
Rat
Rat, Wistar, male 		Hepatocytes HBSS
Wolz E, Liechti H, Notter B, Oesterhelt G, Kistler A.
Characterization of metabolites of astaxanthin in primary cultures of rat hepatocytes ,
Drug Metab Dispos
 27 , 456-62 , 1999
Rat
Rat, Fischer, Lewis and SD, male 10-18 months 		Epithelial HBSS CMF
Herring, A. , Raychaudhuri, R. , Kelley, S. and Iybe, P.
Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats ,
In Vitro
 19 , 576 , 1983
Rat
Rat, SD, 250-300g 		Hepatocytes Krebs-Henseleit
Parasrampuria, R. and Mehvar, R.
Dose-Dependent Inhibition of Transporter-Mediated Hepatic Uptake and Biliary Excretion of Methotrexate by Cyclosporine A in an Isolated Perfused Rat Liver Model. ,
J Pharm Sci
 99 , 5060 , 2010
Rat
Rat, SD, male, 250-350 g 		Hepatocytes RPMI 1640
Dixit, V. , Arthur, M. and Gitnick, G.
Repeated Transplantation of Microencapsulated Hepatocytes for Sustained Correction of Hyperbilirubinemia in Gunn Rats ,
Cell Transplant
 1 , 275 , 1992
Rat
Rat, SD, male, 200 g 		Hepatocytes Medium 199
Rana, B. , Mischoulon, D. , Xie, Y. , Bucher, N. and Farmer, S.
Cell-Extracellular Matrix Interactions Can Regulate the Switch between Growth and Differentiation in Rat Hepatocytes: Reciprocal Expression of C/EBPa and Immediate-Early Growth Response Transcription Factors ,
Mol Cell Biol
 14 (9) , 5858 , 1994
Rat
Rat, Wistar, female, 17 days 		Hepatocytes HBSS
Cotariu, D. , Barr-Nea, L. , Papo, N. and Zaidman, J.
Induction of gamma-Glutamyl Transferase by Dexamethasone in Cultured Rat Hepatocytes ,
Enzyme
 40 , 212 , 1988
Rat
Rat, SD, male, 200-250 g 		Hepatocytes DMEM
Raman Priya, Donkin Shawn S, Spurlock Michael E.
Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures ,
Am J Physiol Regul Integr Comp Physiol
 286 , R206-16 , 2004
Rat
Rat, neonate, 3 day 		Hepatocytes PBS, CMF
Bausher, J. and Schaeffer, W.
A Diploid Rat Liver Cell Culture. 1. Characterization and Sensitivity to Aflatoxin B1 ,
In Vitro
 9 , 286 , 1974
Rat
Rat, Wistar, 220-270g 		Hepatocytes DMEM
Annaert, P. , Turncliff, R. , Booth, C. , Thakker, D. and Brouwer, K.
P-Glycoprotein-Mediated In Vitro Biliary Excretion in Sandwich-Cultured Rat Hepatocytes. ,
Drug Metab Dispos
 29 , 1277-83 , 2001
Rat
Rat, Wistar, adult, male, 150-200 g 		Hepatocytes Williams E
Okumura, T. and Saito, K.
Degradation of Prostaglandin E2 in a Primary Culture of Adult Rat Hepatocytes ,
J. Biochem.
 96 , 429 , 1984
Rat
Rat, SD, male, 350-400 g 		Hepatocytes HBSS
Rodriguez de Turco, E. and Spitzer, J.
Kinetics of Diacylglycerol Accumulation in Response to Vasopressin Stimulation in Hepatocytes of Continuously Endotoxaemic Rats ,
Biochem J
 253 , 73 , 1988
Rat
Rat, SD, male, 200 g 		Hepatocytes Buffers 1 & 2
(see reference)
Rubin, K. , Kjellen, L. and Oslashbrink, B.
Intercellular Adhesion between Juvenile Liver Cells.. A Method to Measure the Formation of Stable Lateral Contacts Between Cells Attached to a Collagen Gel ,
Exp Cell Res
 109 , 413 , 1977
Rat
Rat, Fisher, E14 		Hepatocytes HBSS
Isabel Zvibel, Miri Bronstein, Einav Hubel, Ella Bar-Lev, Zamir Halpern, Ran Oren.
Isolation, characterization and culture of Thy1-positive cells from fetal rat livers ,
World J Gastroenterol
 12 , 3841-7 , 2006
Rat
Rat, SD, albino, male 		Parenchymal Hank's solution, CMF
Bonney, R. , Becker, J. , Walker, P. and Potter, V.
Primary Monolayer Cultures of Adult Rat Liver Parenchymal Cells Suitable for Study of the Regulation of Enzyme Synthesis ,
In Vitro
 9 , 399 , 1974
Rat
Rat 		Hepatocytes and SEC's RPMI
Gopalakrishnan, S. and Harris, E.
In Vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion. ,
J Vis Exp
 57 , e3138 , 2011
Rat
Rat, SD, female, 100-150 g 		Hepatocytes DMEM
(see reference)
Davis, R. , Engelhorn, S. , Pangburn, S. , Weinstein, D. and Steinberg, D.
Very Low Density Lipoprotein Synthesis and Secretion by Cultured Rat Hepatocytes ,
J Biol Chem
 254 (6) , 2010 , 1979
Rat
Rat 		Hepatocytes PBS
Alston-Smith, J. and Pertoft, H
Isolation of Liver Cells: a System for Obtaining Pure Cells in Monolayer Cultures from a Single Rat Liver , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:14.1 , 1995
Rat
Rat, Wistar, 12 day 		Epithelial HBSS, CMF
Malan-Shibley, L. and Iype, P.
Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels ,
Exp Cell Res
 131 , 363 , 1981
Rat
Rat, Fisher 		Epithelial Eagles's MEM
Blouin, M. and Marceau, N.
Primary Culture of Fetal Rat Liver Bipotential Progenitor Cells ,
J Tiss Cul Meth
 13 , 117 , 1991
Rat
Rat, SD, female, 130-160 g 		Hepatocytes HBSS, CF
Johnson, M. , Das, N. , Butcher, F. and Fain, J.
The Regulation of Gluconeogenesis in Isolated Rat Liver Cells by Glucagon, Insulin, Dibutyryl Cyclic Adenosine Monophosphate, and Fatty Acids ,
J Biol Chem
 247 , 3229 , 1972
Rat
Rat, adult 		Hepatocytes William's
Shen, L. , Hillebrand, A. , Wang, D. and Liu, M.
Isolation and Primary Culture of Rat Hepatic Cells. ,
J Vis Exp
 64 , e3917 , 2012
Rat
Rat, SD, male, 200-250 g 		Hepatocytes MEM
Witters, L. , Alberico, L. and Acruch, J.
Insulin Regulation of Glycogen Synthase in the Isolated Rat Hepatocyte ,
Biochem Biophys Res Commun
 69 (4) , 997 , 1976

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.