Adrenal

Species	Cells	Enzyme(s)	Medium	Reference
Bovine Bovine	Chromaffin	Collagenase : 0.05%	Locke's solution, CMF	Aunis, D. , Rotllan, P. and Miras-Portugal, M. Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture , Neurochem Int 7 , 89 , 1985
Bovine Bovine, male	Chromaffin	Collagenase Type 1 : 0.2%	Kreb's Ringer bicarbonate buffer, CF	Schneider, A. , Herz, R. and Rosenheck, K. Stimulus-Secretion Coupling in Chromaffin Isolated From Adrenal Medulla , Proc Natl Acad Sci U S A 74 , 5036 , 1977
Bovine Bovine	Chromaffin	Collagenase : 0.05%	Locke's solution, CMF	Trifaro, J.M. and Lee, R.W. Morphological Characteristics and Stimulus-Secretion Coupling in Bovine Adrenal Chromaffin Cell Cultures , Neuroscience 5 , 1533 , 1980
Bovine Bovine	Chromaffin cells	Collagenase : 0.1% Deoxyribonuclease I : 30 u/ml	DMEM	Hahm, S. , Chen, Y. , Vinson, C. and Eiden, L. A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization. , Mol Pharmacol 64 , 1503 , 2003
Bovine Bovine, adult (also rat, Hanover-Wistar, young; guinea-pig, newborn)	Chromaffin	Collagenase : 0.5% Collagenase : 0.5%	HBSS	Unsicker, K. , Rieffert, B. and Ziegler, W. Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro , Adv Biochem Psychopharmacol 255 , 51 , 1980
Bovine Bovine	Chromaffin	Collagenase Type 1 : 0.1-0.2% Deoxyribonuclease I : 0.003-0.015% Hyaluronidase : 0.015%	DMEM/F12	Dominguez, N. , Rodriguez, M. , Machado, J. and Borges, R. Preparation and Culture of Adrenal Chromaffin Cells. , Methods Mol Biol 846 , 223 , 2012
Bovine Bovine	Chromaffin	Collagenase : 0.25%	F-12 medium	Kumakura, K. , Karoum, F. , Guidotti, A. and Costa, E. Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells , Nature 283 , 489 , 1980
Bovine Bovine	Medulla	Hyaluronidase : 0.2%	Saline w/BSA 0.5%	Knight, D. and Baker, P. Stimulus-Secretion Coupling in Isolated Bovine Adrenal Medullary Cells , Q J Exp Physiol 68 , 123 , 1983
Bovine Bovine	Medulla	Collagenase : 0.05%	Locke's solution, CF	Kilpatrick, D. , Ledbetter, F. , Carson, K. , Kirshner, A. , Slepetis, R. and Kirshner, N. Stability of Bovine Adrenal Medulla Cells in Culture , J Neurochem 35 (3) , 679 , 1980
Bovine Bovine	Medulla	Protease : 0.2%	Saline	Baker, P. and Knight., D Calcium Control of Exocytosis and Endocytosis in Bovine Adrenal Medullary Cells , Phil Trans R Soc Lond 296 , 83 , 1981
Bovine Bovine	Medullary	Collagenase Type 2 : 0.2%	HEPES	Hersey, R. and DiStefano, V. Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured Bovine Adrenal Medullary Cells , J Pharmacol Exp Ther 209 (1) , 147 , 1979
Bovine Bovine	Chromaffin	Collagenase : 0.15%	Kreb's, CMF	Almazan, G. , Aunis, D. , Garcia, A. , Montiel, C. , Nicolas, G. and Sanchez-Garcia, P. Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells , Br J Biomed Sci 81 , 599 , 1984
Bovine Bovine, adult	Medulla	Collagenase Type 1 : 0.5%	HBSS	Unsicker, K. and Griesser, G. Establishment, Characterization and Fibre Outgrowth of Isolated Bovine Adrenal Medullary Cells in Long-Term Cultures , Neuroscience 5 , 1445 , 1980
Bovine Bovine	Medulla	Collagenase : 0.2%	Krebs-Ringer bicarbonate buffer, CMF	Greenberg, A. and Zinder, O. alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells , Cell Tissue Res 226 , 655 , 1982
Bovine Bovine	Chromaffin	Collagenase : 0.05%	DMEM	Aunis, D. , Guerold, B. , Bader, M-F. and Cieselski-Treska, J. Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells , Neuroscience 5 , 2261 , 1980
Bovine Bovine	Chromaffin	Collagenase : 0.2%	Locke's solution	Ortega, J. , Sagen, J. and Pappas, G. Short-term Immunosuppression Enhances Long-term Survival of Bovine Chromaffin Cell Xenografts in Rat CNS , Cell Transplant 1 , 33 , 1992
Bovine Bovine	Chromaffin	Hyaluronidase : 0.2%	HEPES, CF	Brooks, J.C. The Isolated Bovine Adrenomedullary Chromaffin Cell: A Model of Neuronal Excitation-Secretion , Endocrinology 101 , 1369 , 1977
Bovine Bovine	Chromaffin	Collagenase Type 1 : 0.25 %	DMEM	Dahmer, M. , Hart, P. and Perlman, R. Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells , J Neurochem 54 (3) , 931 , 1990
Bovine Bovine	Chromaffin	Deoxyribonuclease I : 15 µg/ml	Medium A (see reference)	Wilson, S.P. and Viveros, O.H. Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium , Exp Cell Res 133 , 159 , 1981
Bovine Bovine	Chromaffin	Deoxyribonuclease I : 30 u/mg	HEPES	Zhu, J. , Li, W. , Toews, M. and Hexum, T. Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in Bovine Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process , J Pharmacol Exp Ther 263 (3) , 1479 , 1992
Bovine Bovine (also rat)	Heart Adrenal chromaffin Paraneurons	Trypsin : 0.06%	25mM HEPES buffered Locke's solution, CMF	Trifaro, J. , Tang, R. and Novas, M. Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons , In Vitro Cell Dev Biol 26 , 335 , 1990
Bovine Bovine	Chromaffin	Collagenase Type 1 : 0.05%	CF Kreb's	Cena, V. , Garcia, A. , Montiel, C. and Sanchez-Garcia, P. Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells , Br J Pharmacol 81 , 119 , 1984
Bovine Bovine	Chromaffin	Collagenase : 0.1%	(see reference)	Pollard, H. , Pazoles, C. , Creutz, C. , Scott, J. , Zinder, O. and Hotchkiss, A. An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells , J Biol Chem 259 , 1114 , 1984
Bovine Bovine	Foreskin	Collagenase : 0.5%	Dulbecco's MEM w/10% calf serum	Folkman, J. , Haudenschild, C. C. and Zetter, B. R. Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76 , 5217 , 1979
Bovine Bovine	Medulla	Collagenase Type 1 : 0.05%	Kreb's, CF	Fenwick, E. , Fajdiga, P. , Howe, N. and Livett, B. Functional and Morphological Characterization of Isolated Bovine Adrenal Medullary Cell , J Cell Biol 76 , 12 , 1978
Bovine Bovine, 6 month	Chromaffin	Collagenase Type 1 : 0.125%	Locke's solution	Moustafa T, Girod S, Tortosa F, Li R, Sol JC, Rodriguez F, Bastide R, Lazorthes Y, Sallerin B. Viability and functionality of bovine chromaffin cells encapsulated into alginate-PLL microcapsules with a liquefied inner core , Cell Transplant 15 , 121-33 , 2006
Bovine Bovine	Chromaffin	Collagenase Type 1 : 0.025%	HBSS, modified	Waymire, J. , Bennett, W. , Boehme, R. , Hankins, L. , Gilmer-Waymire, K. and Haycock, J. Bovine Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture , J Neurosci Methods 7 , 329 , 1983
Bovine Bovine	Chromaffin	Collagenase : 0.2% Deoxyribonuclease I : 0.015%	Locke's solution	Higgins, L. and Berg, D. Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells , J Neurosci 7 (6) , 1792 , 1987
Guinea-Pig Guinea-pig	Adrenal Chromaffin	Collagenase : 0.05%-0.20%	Kreb's-Ringer bicarb glucose buffer, CF	Hochman, J. and Perlman, R.L. Catecholamine Secretion by Isolated Adrenal Cells , Biochim Biophys Acta 421 , 168 , 1976
Guinea-Pig Guinea-pig, 500-700 g	Chromaffin Medulla	Collagenase	BSS (see reference)	Role, L.W. , Leeman, S.E. and Perlman, R.L. Somatostain and Substance P Inhibit Catecholamine Secretion from Isolated Cells of Guinea-pig Adrenal Medulla , Neurochem Int 6 , 1813 , 1981
Guinea-Pig Guinea-pig (also rat, Hanover-Wistar, young; newborn; cattle)	Chromaffin	Collagenase : 0.5% Collagenase : 0.5%	HBSS	Unsicker, K. , Rieffert, B. and Ziegler, W. Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro , Adv Biochem Psychopharmacol 255 , 51 , 1980
Hamster Hamster (Mesocricetus auratus) 100-150 g	Adrenal Chromaffin	Hyaluronidase : 0.20%	Kreb's Ringer bicarbonate buffer	Liang, B.T. and Perlman, R.L. Catecholamine Secretion by Hamster Adrenal Cells , J Neurochem 32 , 927 , 1979
Human Human, adult	Chromaffin	Trypsin : 0.25%	Eagle's MEM	Tischler, A. , DeLellis, R. , Bailes, B. , Nunnemacher, G.,Carabba, V. and Wolfe, H. Nerve Growth Factor-Induced Neurite Outgrowth from Normal Human Chromaffin Cells , Lab Invest 43 , 399 , 1980
Human Human, adult and child	Foreskin	Collagenase : 0.5%	Dulbecco's MEM w/10% calf serum	Folkman, J. , Haudenschild, C. C. and Zetter, B. R. Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76 , 5217 , 1979
Human Human	Chromaffin cells	Collagenase : 0.2%	Locke's solution	Jeon, Y. , Baek, W. , Chung, S. , Shin, N. , Kim, H. and Lee, S. Cultured Human Chromaffin Cells Grafted in Spinal Subarachnoid Space Relieves Allodynia in a Pain Rat Model. , Korean J Anesthesiol 60 , 357 , 2011
Human Human	Adrenocortical	Collagenase Type 1 : 0.2% Deoxyribonuclease I : 0.01%	Krebs Ringer	Caroccia, B. , Fassina, A. , Seccia, T. , Recarti, C. , Petrelli, L. , Belloni, A. , Pelizzo, M. and Rossi, G. Isolation of Human Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method. , Endocrinology 151 , 1375 , 2010
Human Human	Adrenal medulla progenitor	Collagenase : 0.2%	DMEM/F-12	Santana, M. , Chung, K. , Vukicevic, V. , Rosmaninho-Salgado, J. , Kanczkowski, W. , Cortez, V. , Hackmann, K. , Bastos, C. , Mota, A. , Schrock, E. , Bornstein, S. , Cavadas, C. and Ehrhart-Bornstein, M. Isolation, Characterization, and Differentiation of Progenitor Cells from Human Adult Adrenal Medulla. , Stem Cells Transl Med 1 , 783 , 2012
Mouse Mouse, embryonic	Chromafin cells	Papain : 20-25 u/ml	DMEM	Tian Jin-Hua, Wu Zheng-Xing, Unzicker Michael, Lu Li, Cai Qian, Li Cuiling, Schirra Claudia, Matti Ulf, Stevens David, Deng Chuxia, Rettig Jens, Sheng Zu-Hang. The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells , J Neurosci 25 , 10546-55 , 2005
Mouse Mouse	Chromaffin	Collagenase Type 1 : 0.1-0.2% Deoxyribonuclease I : 0.003-0.015% Hyaluronidase : 0.015%	DMEM/F12	Dominguez, N. , Rodriguez, M. , Machado, J. and Borges, R. Preparation and Culture of Adrenal Chromaffin Cells. , Methods Mol Biol 846 , 223 , 2012
Mouse Mouse, 8-10 week	Chromaffin	Papain : 40 u/ml	DMEM	Kolski-Andreaco, A. , Cai, H. , Currle, D. , Chandy, K. and Chow, R. Mouse Adrenal Chromaffin Cell Isolation. , J Vis Exp 2 , 129 , 2007
Ovine Ovine, 3 year	Anterior pituitary	Trypsin : 2.5% Deoxyribonuclease I : 0.004%	DMEM	Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J. The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep , J Endocrinol 162 , 215-25 , 1999
Ovine Ovine, adult and fetal	Adrenocortical	Collagenase Type 1 : 0.4%	DMEM/Ham's F12	Valego, N. and Rose, J. A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in Ovine Adrenocortical Cells. , Reprod Sci 17 , 477 , 2010
Ovine Ovine, fetal	Adrenocortical	Collagenase Type 1 : 0.4%	DMEM/Ham's F12	Valego, N. , Su, Y. , Carey, L. , Young, S. , Tatter, S. , Wang, J. and Rose, J. Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression. , Am J Physiol Regul Integr Comp Physiol 289 , R410 , 2005
Ovine Ovine, adult	Chromaffin cells	Collagenase Type 2 : 0.2% Deoxyribonuclease I : 100 u/ml	Locke's solution	Keating, D. , Rychkov, G. , Adams, M. , Holgert, H. , McMillen, I.C. and Roberts, M. Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels. , J Physiol 555 , 489 , 2004
Rat Rat	Chromaffin	Trypsin : 0.10%	Ham's F-12 w/HEPES	Englert, D.F. An Optical Study of Isolated Rat Adrenal Chromaffin Cells , Exp Cell Res 125 , 369 , 1980
Rat Rat, SD	Chromaffin cells	Collagenase Type 1 : 0.26% Deoxyribonuclease I : 0.015% Hyaluronidase : 0.015%	HBSS	Gilabert, J. Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture , Cell Biology of the Chromaffin Cell , Borges, R. and Gandia, L. , Instituto Teofilo Hernando , 2004
Rat Rat, Fischer, male, 10-16 weeks	Adrenocortical	Deoxyribonuclease I : 0.005%	BSS	Roskelley, C.D. and Auersperg, N. Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture , In Vitro Cell Dev Biol 26 , 493 , 1990
Rat Rat	Chromaffin	Collagenase Type 1 : 0.1-0.2% Deoxyribonuclease I : 0.003-0.015% Hyaluronidase : 0.015%	DMEM/F12	Dominguez, N. , Rodriguez, M. , Machado, J. and Borges, R. Preparation and Culture of Adrenal Chromaffin Cells. , Methods Mol Biol 846 , 223 , 2012
Rat Rat, male, 120-160 g, Rat, SD, male, 400-450 g	Leydig Adrenal	Collagenase Type 2 : 0.03% (adrenal)	Krebs Ringer bicarbonate buffer	Ng, T. and Liu, W. Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis , In Vitro Cell Dev Biol 26 , 24 , 1990
Rat Rat, SD, 2-4 day old (also bovine)	Heart Adrenal chromaffin Paraneurons	Trypsin : 0.06%	25mM HEPES buffered Locke's solution, CMF	Trifaro, J. , Tang, R. and Novas, M. Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons , In Vitro Cell Dev Biol 26 , 335 , 1990
Rat Rat, Fischer, male, 1-10 months	Adrenocortical	Deoxyribonuclease I : 0.005%	BSS	Leonard, R.K. , Auersperg, N. and Parkes, C.O. Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells , In Vitro 19 , 46 , 1983
Rat Rat, SD, male, 400-450 g	Decapular Capsular Glomerulosa	Deoxyribonuclease I : 0.01%	Medium 199	Li, C.H. , Ng, T.B. and Cheng, C.H.K. Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells , Int J Pept Protein Res 19 , 361 , 1982
Rat Rat, Long-Evans, female, 150-200 g	Glomerulosa	Collagenase : 0.2%	MEM-d-Val	Payet, N. , Deziel, Y. and Lehoux, J.-G. Vasopressin: A Potent Growth Factor in Adrenal Glomerulosa Cells in Culture , J Steroid Biochem 20 , 449 , 1984
Rat Rat	Foreskin	Collagenase : 0.5%	Dulbecco's MEM w/10% calf serum	Folkman, J. , Haudenschild, C. C. and Zetter, B. R. Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76 , 5217 , 1979
Rat Rat, Hanover-Wistar, 2nd postnatal week (also guinea-pig, cattle)	Chromaffin	Collagenase : 0.5% Collagenase : 0.5%	HBSS	Unsicker, K. , Rieffert, B. and Ziegler, W. Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro , Adv Biochem Psychopharmacol 255 , 51 , 1980
Rat Rat , SD, female, 200 g	Glomerulosa	Deoxyribonuclease I : 0.05%	Kreb's	Braley, L. , Williams, G. and Bradwin, G. The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells , Endocrinology 106 (1) , 50 , 1980
Rat Rat, Wistar-Hanover, 7-12 day	Medullary	Trypsin : 0.125%	HBSS	Unsicker, K. , Krisch, B. , Otten, U. and Thoenen, H. Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids , Proc Natl Acad Sci U S A 75 (7) , 3498 , 1978
Rat Rat, SD, male	Cortical	Trypsin : 0.25%	Kreb's Ringer bicarbonate buffer	Barofsky, A. , Feinstein, M. and Halkerston, I. Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands , Exp Cell Res 79 , 263 , 1973
Rat Rat, SD, male	ZG ZFR	Collagenase Type 1 : 0.2%	Kreb's	Sayed, S. , Whitehouse, B. and Jones, P. Phosphoserine/Threonine Phosphatases in the Rat Adrenal Cortex: A Role in the Control of Steroidogenesis , J Endocrinol 154 , 449 , 1997
Rat Rat, SD, male	Zona fasciculata/reticularis	Collagenase : 0.4%	Krebs-HEPES	Bruder Eric D, Ball Dennis L, Goodfriend Theodore L, Raff Hershel. An oxidized metabolite of linoleic acid stimulates corticosterone production by rat adrenal cells , Am J Physiol Regul Integr Comp Physiol 284 , R1631-5 , 2003
Rat Rat, Wistar, newborn	Chromaffin cells	Collagenase Type 1 : 0.025% Deoxyribonuclease I : 0.0015%	DMEM	Zhang L, Castell A, Avila E, Drucker-ColÃn R, Escobar A. Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons , J Neuropathol Exp Neurol 59 , 170-4 , 2000
Rat Rat, Holtzman, male, 180-250 g	Adrenal	Collagenase Type 1 : 0.5%	Kreb's Ringer bicarbonate buffer	Kloppenborg, P. , Island, D. , Liddle, G. , Michelakis, A. and Nicholson, W. A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the In Vitro Study of Adrenal Metabolism , Endocrinology 82 , 1053 , 1968
Rat Rat	Chromaffin	Collagenase Type 1 : 0.26% Deoxyribonuclease I : 0.015% Hyaluronidase : 0.015%	HBSS	Gilabert, J, Montalvo, G. and Artalejo A. Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays , Nat Protoc , 294 , 2006