Kidney

Species	Cells	Enzyme(s)	Medium	Reference
Avian Avian, house sparrows	Tubule segments	Collagenase Type 2 : 0.1%	HBSS	Goldstein D. , Reddy V. and Plaga K. Second Messenger Production in Avian Medullary Nephron Segments in Response to Peptide Hormones , Am J Physiol 276 , R847 , 1999
Avian Avian, chicken	Tubule and glomeruli	Collagenase Type 1 : 0.1%	RPMI 1640	Reich, C. and Bonar, R. Separation of Avian Kidney Tubules and Glomeruli for In Vitro Culture , Meth Cell Sci 7 , 97 , 1982
Bovine Bovine	Papillary duct	Hyaluronidase : 0.2%	Keri's buffer HEPES buffered saline	Husted, R. , Hayashi, M. and Stokes, J. Characteristics of Papillary Collecting Duct Cells in Primary Culture , Am J Physiol 255 , F1160 , 1988
Canine Dog, 1-5 months	Proximal tubules	Collagenase Type 1 : 0.15%	Krebs Ringer bicarbonate buffer	States, B. , Reynolds, R. , Lee, J. and Segal, S. Cystine Uptake By Cultured Cells Originating From Dog Proximal Tubule Segments , In Vitro Cell Dev Biol 26 , 105 , 1990
Canine Dog, mongrel, 20 Kg	Proximal tubular	Deoxyribonuclease I : 0.0125%	(see reference)	Yau, C. , Rao, L. and Silverman, M. Sugar Uptake Into a Primary Culture of Dog Kidney Proximal Tubular Cells , Can J Physiol Pharmacol 63 , 417 , 1985
Canine Canine	Renal adipose derived cells	Collagenase Type 1 : 0.3%	DMEM	Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J. Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. , Lipids Health Dis 10 , 171 , 2011
Fish Winter flounder (Pseudopleur-onectes americanus) 200 - 500 g	Renal tubule	Trypsin : 0.2% Trypsin : 0.2%	CMF solution	Dickman, K. and Renfro, J. Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport , Am J Physiol 251 , 424 , 1986
Guinea-Pig Guinea-pig, male, 100 g	Single cells	Trypsin : 0.25%	CF salt solution	Phillips, H. Dissociation of Single Cells from Lung or Kidney Tissue with Elastase , In Vitro 8 , 101 , 1972
Hamster Hamster (also rat, SD and Wistar, 150-225 g, rabbit, bovine)	Papillary duct	Hyaluronidase : 0.2%	Keri's buffer HEPES buffered saline	Husted, R. , Hayashi, M. and Stokes, J. Characteristics of Papillary Collecting Duct Cells in Primary Culture , Am J Physiol 255 , F1160 , 1988
Human Human	Renal cells	Collagenase Type 4 : 300 u/ml Neutral Protease : see reference	HBSS	Presnell, S. , Bruce, A. , Wallace, S. , Choudhury, S. , Genheimer, C. , Cox, B. , Guthrie, K. , Werdin, E. , Tatsumi-Ficht, P. , Ilagan, R. , Kelley, R. , Rivera, E. , Ludlow, J. , Wagner, B. , Jayo, M. and Bertram, T. Isolation, Characterization, and Expansion Methods for Defined Primary Renal Cell Populations from Rodent, Canine, and Human Normal and Diseased Kidneys. , Tiss Eng 17 , 261 , 2011
Human Human	Papillary duct	Collagenase : 400 u/ml	Eagle's MEM-HEPES buffer w/L-glutamine	Trifillis, A. and Kahng, M. Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells , In Vitro Cell Dev Biol 26 , 441 , 1990
Human Human, adult, 14-66 years	Tubular	Collagenase : 100 u/ml	Joklik's MEM	Trifillis, A. , Regec, A. and Trump, B. Isolation, Culture, and Charaterization of Human Renal Tubular Cells , J Urol 133 , 324 , 1985
Human Human	Mesangial	Trypsin : 0.25%	DMEM/Ham's F-12	Heieren, M. , van der Woude, F. and Balfour Jr., H. Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells , Proc Natl Acad Sci U S A 85 , 1642 , 1988
Human Human, adult	Tubular	Collagenase : 250 u/ml	PBS	Yang, A. , Gould-Kostka, J. and Oberley, T. In Vitro Growth and Differentiation of Human Kidney Tubular Cells on a Basement Membrane Substrate , In Vitro Cell Dev Biol 23 (1) , 34 , 1987
Human Human	Malignant Stromal	Papain : 0.009%	Sacks solution	Hemstreet, G. , Enoch, P. and Pretlow, T. Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification , Cancer Res 40 , 1043 , 1980
Human Human, 24 newborn and stillborn (also rabbit)	Renal	Trypsin : 0.25%	See reference	De Oca, H. and Malinin, T. Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys , J Clin Microbiol 2 , 243 , 1975
Human Human	Renal cortex	Trypsin : 0.1%	Tissue Culture Grade Water	McAteer, J, Kempson, S. and Evan, A Culture of Human Renal Cortex Epithelial Cells , J Tiss Cul Meth 13 , 143 , 1991
Human Human, adult	Renal proximal tubule and cortical fibroblasts	Collagenase Type 2 : 383 u/ml	DMEM/F-12	Johnson, D. , Saunders, H. , Johnson, F. , Huq, S. , Field, M. and Pollock, C. Cyclosporin exerts a direct fibrogenic effect on human tubulointerstitial cells: roles of insulin-like growth factor I, transforming growth factor beta1, and platelet-derived growth factor , J Pharmacol Exp Ther 289 , 535-42 , 1999
Human Human	Renal adipose derived cells	Collagenase Type 1 : 0.3%	DMEM	Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J. Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. , Lipids Health Dis 10 , 171 , 2011
Human Human, adult	Renal tumor cells and proximal tubular epithelial	Collagenase Type 2 : 0.1%	DMEM/F12	Valente, M. , Henrique, R. , Costa, V. , Jeronimo, C. , Carvalho, F. , Bastos, M. , Guedes de Pinho, P. and Carvalho, M. A Rapid and Simple Procedure for the Establishment of Human Normal and Cancer Renal Primary Cell Cultures from Surgical Specimens. , PLoS ONE 6 , e19337 , 2011
Monkey Monkey	Kidney	Trypsin : 0.01% - 0.00001%	Eagle's MEM	Melnick, J. and Wallis, C. Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells , Dev Biol 37 , 77 , 1976
Mouse Mouse	Kidney mesenchymal	Collagenase : 250 u/ml Neutral Protease : 33.3 u/ml	DMEM/F12	Plotkin, M. and Goligorsky, M. Mesenchymal Cells from Adult Kidney Support Angiogenesis and Differentiate into Multiple Interstitial Cell Types including Erythropoietin-Producing Fibroblasts. , Am J Physiol Renal Physiol 291 , F902-12 , 2006
Mouse Mouse, adult	Kidney sphere-derived	Collagenase Type 4 : 0.1%	Iscove's Modified Dulbecco's	Huang, Y. , Johnston, P. , Zhang, B. , Zakari, A. , Chowdhry, T. , Smith, R. , Marban, E. , Rabb, H. and Womer, K. Kidney-Derived Stromal Cells Modulate Dendritic and T Cell Responses. , J Am Soc Nephrol 20 , 831-41 , 2009
Mouse Mouse, naive SJL (H-2S)	Proximal tubular epithelial	Deoxyribonuclease I : 15 µg/ml	RPMI 1640	Haverty, T. , Kelly, C. , Hines, W. , Amenta, P. , Watanabe, M. , Harper, R. , Kefalides, N. and Neilson, E. Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis , J Cell Biol 107 , 1359 , 1988
Mouse Mouse, 10-12 week	Kidney	Collagenase Type 4 : 0.075%	DMEM	Dekel, B. , Zangi, L. , Shezen, E. , Reich-Zeliger, S. , Eventov-Friedman, S. , Katchman, H. , Jacob-Hirsch, J. , Amariglio, N. , Rechavi, G. , Margalit, R. and Reisner, Y. Isolation and Characterization of Nontubular sca-1+lin- Multipotent Stem/Progenitor Cells from Adult Mouse Kidney. , J Am Soc Nephrol 17 , 3300 , 2006
Mouse Mouse	Epithelial	Collagenase Type 4 : 1%	DMEM /F-12	Taub, M. Renal Tubule Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley & Sons, Ltd. , 14B:6.1 , 1995
Mouse Mouse	Glomeruli	Collagenase Type 1 : 0.1% Deoxyribonuclease I : 100 u/ml	DMEM	Sedrakyan, S. , Da Sacco, S. , Milanesi, A. , Shiri, L. , Petrosyan, A. , Varimezova, R. , Warburton, D. , Lemley, K. , De Filippo, R. and Perin, L. Injection of Amniotic Fluid Stem Cells Delays Progression of Renal Fibrosis. , J Am Soc Nephrol 23 , 661-73 , 2012
Mouse Mouse, 6 week	Glomerular mesangial cells	Collagenase Type 4 : 0.1%	RPMI 1640	Radeke HH, Janssen-Graalfs I, Sowa EN, Chouchakova N, Skokowa J, Loscher F, Schmidt RE, Heeringa P, Gessner JE. Opposite regulation of type II and III receptors for immunoglobulin G in mouse glomerular mesangial cells and in the induction of anti-glomerular basement membrane (GBM) nephritis , J Biol Chem 277(30) , 27535 , 2002
Mouse Mouse	Kidney	Deoxyribonuclease I : 125 u/ml Protease, Bacillus Licheniformis : 1%	DPBS	Adam, M. , Potter, A. and Potter, S. Psychrophilic Proteases Dramatically Reduce Single-Cell RNA-Seq Artifacts: A Molecular Atlas of Kidney Development. , Development 144 , 3625-3632 , 2017
Mouse Mouse, 3-7 month	Fibroblasts, mesangial, smooth muscle	Trypsin : 0.25% Collagenase : see reference Soybean Trypsin Inhibitor : 0.05%	DMEM	Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH. Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation , Mol Biol Cell 10 , 1569-79 , 1999
Mouse Mouse	Mactrophages	Collagenase Type 4 : 0.1% Deoxyribonuclease I : 0.01%	DMEM	Yuan, Y. , Li, L. , Zhu, L. , Liu, F. , Tang, X. , Liao, G. , Liu, J. , Cheng, J. , Chen, Y. and Lu, Y. Mesenchymal Stem Cells Elicit Macrophages into M2 Phenotype Via Improving Transcription Factor EB-Mediated Autophagy to Alleviate Diabetic Nephropathy. , Stem Cells 38 , 639-652 , 2020
Mouse Mouse, adult	Cortex, proximal tubule	Collagenase : 0.15%	DMEM	Syal Ashu, Schiavi Susan, Chakravarty Sumana, Dwarakanath Vangipuram, Quigley Raymond, Baum Michel. Fibroblast growth factor-23 increases mouse PGE2 production in vivo and in vitro , Am J Physiol/Renal 290 , F450-5 , 2006
Mouse Mouse, 4 week	Endothelial kidney	Collagenase Type 1 : 0.1%	DMEM	Kondo, S. , Scheef, E. , Sheibani, N. and Sorenson, C. PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis. , Am J Physiol Cell Physiol 292 , C2070 , 2007
Mouse Mouse, male, 8-16 week	Renal tubular epithelial	Collagenase : 200 u/ml Soybean Trypsin Inhibitor : see reference	HBSS	Breggia, A. and Himmelfarb, J. Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. , Oxid Med Cell Longev 1 , 33 , 2008
Mouse Mouse	T cells	Collagenase Type 1 : 0.1% Collagenase Type 2 : 0.2% Elastase : 2 u/ml Soybean Trypsin Inhibitor : 0.025% Hyaluronidase : 50 u/ml	RPMI	Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E. Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. , Bio Protoc 13 , e4679 , 2023
Mouse Mouse	Proximal tubule	Collagenase Type 2 : 0.013%	see reference	Wright, J. , Morales, M. , Sousa-Menzes, J. , Ornellas, D. , Sipes, J. , Cui, Y. , Cui, I. , Hulamm, P. , Cebotaru, V. , Cebotaru, L. , Guggino, W. and Guggino, S. Transcriptional Adaptation to Clcn5 Knockout in Proximal Tubules of Mouse Kidney. , Physiol Genomics 33 , 341 , 2008
Mouse Mouse, male, 12-16 week	Renal proximal tubule cells	Collagenase Type 2 : 0.1% Soybean Trypsin Inhibitor : 0.25%	DMEM/F-12	Cunningham, R. , Xiaofei, E. , Steplock, D. , Shenolikar, S. and Weinman, E. Defective PTH Regulation of Sodium-Dependent Phosphate Transport in NHERF-1-/- Renal Proximal Tubule Cells and Wild-Type Cells Adapted to Low-Phosphate Media. , Am J Physiol Renal Physiol 289 , F933 , 2005
Mouse Mouse, male, 2 month	Cortical collecting duct	Collagenase : 54-178 u/ml Protease : see reference	MEM	Sindic, A. , Velic, A. , Basoglu, C. , Hirsch, J. , Edemir, B. , Kuhn, M. and Schlatter, E. Uroguanylin and Guanylin Regulate Transport of Mouse Cortical Collecting Duct Independent of Guanylate Cyclase C. , Kidney Int 68 , 1008 , 2005
Mouse Mouse	Glomeruli	Collagenase Type 4 : 0.1%	RPMI 1640	Kabgani, N. , Grigoleit, T. , Schulte, K. , Sechi, A. , Sauer-Lehnen, S. , Tag, C. , Boor, P. , Kuppe, C. , Warsow, G. , Schordan, S. , Mostertz, J. , Chilukoti, R. , Homuth, G. , Endlich, N. , Tacke, F. and Weiskirchen, R. Primary Cultures of Glomerular Parietal Epithelial Cells or Podocytes with Proven Origin. , PLoS ONE 7 , e34907 , 2012
Mouse Mouse, 10-14 week	Proximal tubule	Collagenase Type 1 : 0.1%	See reference	Park, J. , Pasupulati, R. , Feldkamp, T. , Roeser, N. and Weinberg, J. Cyclophilin D and the Mitochondrial Permeability Transition in Kidney Proximal Tubules after Hypoxic and Ischemic Injury. , Am J Physiol/Renal 301 , F134 , 2011
Mouse Mouse, embryonic	Kidney	Collagenase Type 1 : 0.25% Pancreatin : 1.0% Deoxyribonuclease I : 1 u/ml	HBSS	Brown, A. , Blank, U. , Adams, D. , Karolak, M. , Fetting, J. , Hill, B. and Oxburgh, L. Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney. , J Vis Exp 50 , e2555 , 2011
Mouse Mouse	Collecting duct epithelial	Collagenase Type 2 : 0.1%	DMEM	DiRocco, D. , Kobayashi, A. , Taketo, M. , McMahon, A. and Humphreys, B. Wnt4/Î²-catenin Signaling in Medullary Kidney Myofibroblasts. , J Am Soc Nephrol 24 , 1399 , 2013
Porcine Porcine, hybrid	Kidney	Collagenase Type 1 : 0.1%	HBSS	Heussner, A. and Dietrich, D. Primary Porcine Proximal Tubular Cells as an Alternative to Human Primary Renal Cells in Vitro: an Initial Characterization. , BMC Cell Biol 14 , 55 , 2013
Rabbit Rabbit (also rat, SD and Wistar, 150-225 g, hamster, bovine)	Papillary duct	Hyaluronidase : 0.2%	Keri's buffer HEPES buffered saline	Husted, R. , Hayashi, M. and Stokes, J. Characteristics of Papillary Collecting Duct Cells in Primary Culture , Am J Physiol 255 , F1160 , 1988
Rabbit Rabbit, New Zealand white, female, 2-3 Kg	Renal proximal tubules	Deoxyribonuclease I : 70 u/ml	Modified DME-F12	Rodeheaver, D. , Aleo, M. and Schnellmann, R. Differences in Enzymatic and Mechanical Isolated Rabbit Renal Proximal Tubules: Comparison in Long-Term Incubation , In Vitro Cell Dev Biol 26 , 898 , 1990
Rabbit Rabbit, New Zealand, white, female, 2.5 Kg	Papillary collecting duct	Collagenase : 0.2%	(see reference)	Dworzack, D. and Grantham, J. Preparation of Renal Papillary Collecting Duct Cells for Study , Nat Neurosci 8 , 191 , 1975
Rabbit Rabbit, New Zealand, white	Collecting tubule	Trypsin : 0.05%	Kreb's Ringer buffer	Grenier, F. and Smith, W. Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae , Prostaglandins 16 , 759 , 1978
Rabbit Rabbit, New Zealand, white, 2-3 kg	MTALH cells RCCT cells	Collagenase : 0.1%	DMEM	Allen, M. , Nakao, A. , Sonnenburg, W. , Burnatowska-Hledin, M. , Speilman, W. and Smith, W. Immunodissection of Cortical and Medullary Thick Ascending Limb Cells From Rabbit Kidney , Am J Physiol 255 , F704 , 1988
Rabbit Rabbit, New Zealand white, male, 1.5 - 2.0 Kg	Duct	Soybean Trypsin Inhibitor : 0.025%	Hank's Solution with calcium and HEPES	Naray-Fejes-Toth, A. and Fejes-Toth, G. Immunoelection and Culture of Cortical Collecting Duct Cells , J Tiss Cul Meth 13 , 179 , 1991
Rabbit Rabbit, New Zealand White, female	Nephron segments	Collagenase Type 2 : 0.05% Deoxyribonuclease I : 50 u/ml	Eagle's MEM	Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E. A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments , Am J Physiol 273 , F650 , 1997
Rabbit Rabbit, New Zealand	Proximal tubule cells	Collagenase Type 4 : 0.005% Soybean Trypsin Inhibitor : 0.00025%	DMEM/F-12	Taub, M. Primary Kidney Proximal Tubule Cells. , Methods Mol Biol 290 , 231 , 2005
Rabbit Rabbit	Renal proximal tubular	Collagenase Type 1 : 60 u/ml	DMEM/F12	Nowak, G. and Bakajsova, D. Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes. , J Vis Exp 71 , e4301 , 2013
Rat Rat,Wistar, 300-400 gm	Proximal tubules	Collagenase : 0.15 %	Krebs Henseleit solution	Vinay, P. , Gougoux, A. and Lemieux, G. Isolation of a Pure Suspension of Rat Proximal Tubules , Am J Physiol 241 , F403 , 1981
Rat Rat, SD, 225-250 g	Glomerular mesangial cells	Collagenase Type 1 : 250 u/ml	DMEM	Amiri F, Garcia R. Regulation of angiotensin II receptors and PKC isoforms by glucose in rat mesangial cells , Am J Physiol 276 , F691-9 , 1999
Rat Rat, adult	Inner medullary collecting duct Papillae	Collagenase Type 2 : 0.1%	PBS	Brion, L. , Schwartz, J. , Lachman, H. , Zavilowitz, B. and Schwartz, G. Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells , Am J Physiol 257 , F486 , 1989
Rat Rat, SD, male	Proximal tubule suspensions	Collagenase Type 4 : 0.1% Pronase E : 2 u/ml	HEPES buffer	Eitle E, Hiranyachattada S, Wang H, Harris PJ. Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney , Am J Physiol 274 , C1075-80 , 1998
Rat Rat, Wistar, female, 100-150 g	Glomerular mesangial	Collagenase : 0.025%	RPMI 1640	Wang, J. , Kester, M. and Dunn, M. The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells , Biochim Biophys Acta 969 , 217 , 1988
Rat Rat, Wistar, 300g	Renal proximal tubules	Collagenase Type 2 : 0.2%	DMEM/F12	Deng Aihua, Miracle Cynthia M, Lortie Mark, Satriano Joseph, Gabbai Francis B, Munger Karen A, Thomson Scott C, Blantz Roland C. Kidney oxygen consumption, carbonic anhydrase, and proton secretion , Am J Physiol/Renal 290 , F1009-15 , 2006
Rat Rat, male, 150-200 g	Epithelial Proximal tubule	Protease : 0.1%	HBSS/CMF	Elliget, K. and Trump, B. Primary Cultures of Normal Rat Kidney Proximal Tubule Epithelial Cells for Studies of Renal Cell Injury , In Vitro Cell Dev Biol 27 , 739 , 1991
Rat Rat, Wistar, male, 300-350 g	Renal proximal tubules	Collagenase Type 2 : 0.2%	DMEM/F-12	Deng, A. , Miracle, C. , Suarez, J. , Lortie, M. , Satriano, J. , Thomson, S. , Munger, K. and Blantz, R. Oxygen Consumption in the Kidney: Effects of Nitric Oxide Synthase Isoforms and Angiotensin II. , Kidney Int 68 , 723 , 2005
Rat Rat	Fibroblasts Kidney	Trypsin : up to 0.25%	Dulbecco-Vogt MEM	Wallach, D. , Anderson, W. and Pastan, I. Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin , J Biol Chem 253 , 24 , 1978
Rat Rat	Proximal tubule	Collagenase Type 2 : 0.1%	see reference	Panico, C. , Luo, Z. , Damiano, S. , Artigiano, F. , Gill, P. and Welch, W. Renal Proximal Tubular Reabsorption is Reduced in Adult Spontaneously Hypertensive Rats: Roles of Superoxide and Na+/H+ Exchanger 3. , Hypertension 54 , 1291 , 2009
Rat Rat, Wistar, male, 150 - 200 g	Renal target	Collagenase : 1.0% (also 0.7%)	Eagle's MEM	Barlet-Bas, C. , Khadouri, C. , Marsey, S. and Doucet, A. Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine , Proc Natl Acad Sci U S A 85 , 1707 , 1988
Rat Rat, Lewis, male	Renal adipose derived cells	Collagenase Type 1 : 0.3%	DMEM	Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J. Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. , Lipids Health Dis 10 , 171 , 2011
Rat Rat, Wistar, male, adult	Proximal tubules	Collagenase : 0.2%	Krebs-Henseleit buffer	Gesek, F. , Wolff, D. and Strandhov, J. Improved Separation Method for Rat Proximal and Distal Renal Tubules , Am J Physiol 253 , F358 , 1987
Rat Rat, SD, fetal	Fetal kidney	Collagenase Type 4 : 0.1%	MEM	Gupta, A. , Jadhav, S. , Tripathy, N. and Nityanand, S. Fetal Kidney Cells Can Ameliorate Ischemic Acute Renal Failure in Rats through Their Anti-Inflammatory, Anti-Apoptotic and Anti-Oxidative Effects. , PLoS ONE 10 , e0131057 , 2015
Rat Rat, SD, 250-300 g	Microvessels	Collagenase Type 2 : 0.1%	PSS	Li, N. , Teggatz, E. , Li, P. , Allaire, R. and Zou, A. Formation and Actions of Cyclic ADP-Ribose in Renal Microvessels , Microvasc Res 60 , 149 , 2000
Rat Rat, 12-weeks-old	Renal	Collagenase : 0.1%	DMEM	Ishikawa, S. , Kusaka, I. , Higashiyama, M. , Nagasaka, S. , Saito, T. , Honda, K. and Saito, T. Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein , Nat Neurosci 50 , 1506 , 1996
Rat Rat, SD, male	Nephron segments	Collagenase Type 2 : 0.05% Deoxyribonuclease I : 50 u/ml	Eagle's MEM	Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E. A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments , Am J Physiol 273 , F650 , 1997
Rat Rat	Cortical tubule cells	Collagenase : 0.6%	DMEM	Arystarkhova, E. , Wetzel R. and Sweadner K. Distribution and Oligomeric Association of Splice Forms of Na(+)-K(+)-ATPase Regulatory Gamma-subunit in Rat Kidney , Am J Physiol/Renal 282(3) , F393 , 2002
Rat Rat, SD, 1 week	Cortical collection duct	Collagenase Type 2 : 0.15%	DMEM/Ham's F12	Valencia L. , Bidet M. , Martial S. , Sanchez E. , Melendez E. , Tauc M. , Poujeol C. , Martin D. , Namorado M. , Reyes J. and Poujeol P. Nifedipine-activated Ca(2+) Permeability in Newborn Rat Cortical Collecting Duct Cells in Primary Culture , Am J Physiol Cell Physiol 280(5) , C1193 , 2001
Rat Rat, SD, male, 5-6 week	Renal tubules	Collagenase : 0.1%	see reference	Miyata, N. , Park, F. , Li, X. and Cowley, A. Distribution of Angiotensin AT1 and AT2 Receptor Subtypes in the Rat Kidney , Am J Physiol 277 , F437 , 1999
Rat Rat, SD, 250-300 g	Renal	Collagenase Type 2 : 0.2%	see reference	Mattson D. and Wu F. Nitric Oxide Synthase Activity and Isoforms in Rat Renal Vasculature , Hypertension 35 , 337 , 2000
Rat Rat, SD, male, 200-250g	Medullary thick ascending limb	Collagenase : 0.1%	HEPES-saline	Silva, G. , Beierwaltes, W. and Garvin, J. Extracellular ATP stimulates NO production in rat thick ascending limb , Hypertension 47 , 563-7 , 2006
Rat Rat, SD, male 275-300 g	Glomerular mesangial cells	Collagenase Type 1 : 250 u/ml	DMEM	Amiri Farhad, Shaw Sean, Wang Xiaodan, Tang Jie, Waller Jennifer L, Eaton Douglas C, Marrero Mario B. Angiotensin II activation of the JAK/STAT pathway in mesangial cells is altered by high glucose , Kidney Int Suppl 61 , 1605-16 , 2002