Thymus

Species	Cells	Enzyme(s)	Medium	Reference
Human Human	Dentritic	Collagenase Type 2 : 0.1% Deoxyribonuclease I : 0.002%	RPMI 1640	Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K. Human thymus contains 2 distinct dendritic cell populations , Blood 97 , 1733-41 , 2001
Mouse Mouse	Dentritic	Collagenase Type 3 : 0.1% Deoxyribonuclease I : 325 u/ml	RPMI 1640	Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells , J Exp Med 196 , 1415 , 2002
Mouse Mouse, 3-6 week	Thymic	Collagenase Type 3 : 100-400 u/ml	HBSS	Smith KM, Olson DC, Hirose R, Hanahan D. Pancreatic gene expression in rare cells of thymic medulla: evidence for functional contribution to T cell tolerance , Int Immunol 9 , 1355-65 , 1997
Mouse Mouse	Thymic stromal	Collagenase Type 4 : 0.02% Neutral Protease : 0.02% Deoxyribonuclease I : 0.0025%	RPMI-1640	Rode, I. and Boehm, T. Regenerative Capacity of Adult Cortical Thymic Epithelial Cells. , Proc Natl Acad Sci U S A 109 , 3463-8 , 2012
Mouse Mouse, Swiss, 6 wk	Thymus	Collagenase Type 3 : 150 u/ml	DMEM	Jones, K. and Pierre, R. Analysis of Cellular Heterogeneity in Mouse Thymus Cultures , In Vitro 17 , 431 , 1981
Mouse Mouse, C3H, 16 wk old, female	Epithelial	Collagenase Type 3 : 0.1%	DMEM	Ehmann, U. , Shiurba, R. and Peterson, W. Long-Term Proliferation of Mouse Thymic Epithelial Cells in Culture , In Vitro Cell Dev Biol 22 (12) , 738 , 1986
Mouse Mouse (BALB/c, C3H, C57BL/6), 1-28 day	Epithelial, thymus	Neutral Protease : 1.5 µg/ml	DMEM	Ropke, C. , van Deurs, B. and Petersen, O. Short-term Cultivation of Murine Thymic Epithelial Cells in a Serum-Free Medium , In Vitro Cell Dev Biol 26 , 671 , 1990
Mouse Mouse	Stromal	Collagenase Type 3 : 0.2% Hyaluronidase : 0.1%	RPMI 1640	Phillips, Joy, Brondstetter, T. , English, C. , Lee, H. , Virts, E. and Thoman, M. IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution , J Immunol , 4869 , 2004
Rat Rat (ACI/NMs X BUF/Mna) F1, male, 28 months Rat, (ACI/MNs) male, 8 weeks (also bovine, adult)	Epthelial	Collagenase Type 3 : 0.1%	Eagle's MEM Serum-free	Masuda, A. , Ohtsuka, K. and Matsuyama, M. Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus , In Vitro Cell Dev Biol 26 , 713 , 1990
Rat Rat	Thymic epithelial	Collagenase Type 3 : 0.1%	DMEM	Masuda, A. and Matsuyama, M Epithelial Cell Lines From Rat Thymoma and Rat Thymus , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 21C:4.1 , 1995
Rat Rat, postnatal	Thymic	Trypsin : 0.05%	HBSS	Bonfanti, P. , Claudinot, S. , Amici, A. , Farley, A. , Blackburn, C. and Barrandon, Y. Microenvironmental Reprogramming of Thymic Epithelial Cells to Skin Multipotent Stem Cells. , Nature 466 , 978 , 2010