Thyroid/Parathyroid

Species	Cells	Enzyme(s)	Medium	Reference
Bovine Bovine	Parathyroid glands	Deoxyribonuclease I : 0.0075%	HEPES buffer	Wallace, J. and Scarpa, A. Regulation of Parathyroid Hormone Secretion in Vitro by Divalent Cations , J Biol Chem 257 , 10613 , 1982
Bovine Bovine (also porcine)	Thyroid	Trypsin : 0.004%	EBSS	Tong, W. The Isolation and Culture of Thyroid Cells , Meth Enzymol 32 , 745 , 1974
Bovine Bovine, adult	Parathyroid	Deoxyribonuclease I : 0.005%,	HEPES Ham's F10	Nygren, P. , Gylfe, E. , Larsson, R. , Johansson, H. , Juhlin, C. , Klareskoq, L. , Akerstrom, G. and Rastad, J. Modulation of the Ca2+-Sensing Function of Parathyroid Cells In Vitro and in Hyperparathyroidism , Biochim Biophys Acta 968 , 253 , 1988
Bovine Bovine	Parathyroid	Deoxyribonuclease I : 0.004%	Eagle's #2 medium without bicarbonate	Brown, E. , Hurwitz, S. and Aurbach, G. Preparation of Viable Isolated Bovine Parathyroid Cells , Endocrinology 99 , 1582 , 1976
Chicken Chick embryo	Thyroid Muscle Heart	Collagenase : 0.25%	Tyrode's saline, potassium free	Hilfer, S. and Brown, J. Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart , Exp Cell Res 65 , 246 , 1971
Chicken Chicken, Rhode Island Red, embryo	Thyroid follicular	Collagenase : 0.2%	Tyrode's solution, CMF	Spooner, B. The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture , J Cell Physiol 75 , 33 , 1970
Human Human	Thyroid	Collagenase : 300 u/ml	Ham's F-12/MEM	Miller, R. , Hiraoka, T. , Nakamura, N. , Tenou, H. , Kopecky, K. , Jones, M. and Gould, M. In Vitro Culture of Human Thyroid Cells; Methods and Application to Radiation Biology. , J Radiat Res 26 , 269 , 1985
Human Human	Endothelial	Collagenase Type 2 : 0.1%	DMEM	Patel, V. , Logan, A. , Watkinson, J. , Uz-Zaman, S. , Sheppard, M. , Ramsden, J. and Eggo, M. Isolation and Characterization of Human Thyroid Endothelial Cells. , Am J Physiol Endocrinol Metab 284 , E168 , 2003
Human Human	Thyrocytes	Neutral Protease : 0.5% Trypsin : 0.25% Collagenase : 0.1%	EBSS	Howie, A. , Walker, S. , Akesson, B. , Arthur, J. and Beckett, G. Thyroidal Extracellular Glutathione Peroxidase: A Potential Regulator of Thyroid-Hormone Synthesis. , Biochem J 308 ( Pt 3) , 713 , 1995
Human Human	Thyrocytes	Collagenase Type 1 : 130 u/ml Neutral Protease : 0.5 u/ml	HBSS	Gianoukakis, A. , Cao, H. , Jennings, T. and Smith, T. Prostaglandin Endoperoxide H Synthase Expression in Human Thyroid Epithelial Cells. , Am J Physiol Cell Physiol 280 , C701 , 2001
Human Human	Thyroid normal and tumor	Trypsin : 0.125% Collagenase Type 4 : 300 u/ml	RPMI-1640	Yi, S. , Kim, H. , Koo, B. , Lee, S. , Choi, J. and Kang, Y. Efficient Dissociation Protocol for Generation of Single Cell Suspension from Human Thyroid Tissue for Single Cell RNA Sequencing. , Endocrinol Metab (Seoul) Vol. 37 , 698-700 , 2022
Mouse Mouse, 6 week	Thyroid	Neutral Protease : 0.0012 u/ml Collagenase Type 2 : 0.25 u/ml	RPMI 1640	Martin, A. , Coronel, E. , Sano, G. , Chen, S. , Vassileva, G. , Canasto-Chibuque, C. , Sedgwick, J. , Frenette, P. , Lipp, M. , Furtado, G. and Lira, S. A Novel Model for Lymphocytic Infiltration of the Thyroid Gland Generated by Transgenic Expression of the CC Chemokine CCL21. , J Immunol 173 , 4791 , 2004
Mouse Mouse	Thyroid tumor	Collagenase Type 1 : 300 u/ml Hyaluronidase : 100 u/ml Deoxyribonuclease I : 0.01%	DMEM	Kitajima, S. , Kohno, S. , Kondoh, A. , Sasaki, N. , Nishimoto, Y. , Li, F. , Mohammed, M. , Muranaka, H. , Nagatani, N. , Suzuki, M. , Kido, Y. and Takahashi, C. Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts. , Stem Cells 33 , 1657-69 , 2015
Ovine Sheep	Thyroid	Collagenase : 0.2%	Puck's Saline F	Kerkof, P. Preparation of Primary Cultures of Ovine Thyroid Gland Cells , J Tiss Cul Meth 7 , 23 , 1982
Porcine Porcine (also bovine)	Thyroid	Trypsin : 0.004%	EBSS	Tong, W. The Isolation and Culture of Thyroid Cells , Meth Enzymol 32 , 745 , 1974
Rat Rat, Lewis, male, 4 week	Thyroid	Collagenase Type 2 : 0.15% Collagenase Type 4 : 0.15%	DMEM	Arauchi, A. , Shimizu, T. , Yamato, M. , Obara, T. and Okano, T. Tissue-Engineered Thyroid Cell Sheet Rescued Hypothyroidism in Rat Models After Receiving Total Thyroidectomy Comparing with Nontransplantation Models. , Tissue Eng Part A 15 , 3943 , 2009