Parotid

Species	Cells	Enzyme(s)	Medium	Reference
Mouse Mouse	Parotid and sublingual glandular	Neutral Protease : 50 u/ml Collagenase Type 1 : 100 u/ml	PBS	Ogawa, M. , Oshima, M. , Imamura, A. , Sekine, Y. , Ishida, K. , Yamashita, K. , Nakajima, K. , Hirayama, M. , Tachikawa, T. and Tsuji, T. Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ. , Nat Commun 4 , 2498 , 2013
Rat Rat	Parotid	Hyaluronidase : 0.025%	HBSS with 20mM HEPES	Takuma, T. and Ichida, T. Amylase Secretion From Saponin-Permeabilized Parotid Cells Evoked by Cyclic AMP , J Invest Dermatol 103 , 95 , 1988
Rat Rat, SD, 100-120 g	Parotid acinar cells	Collagenase Type 2 : 90 u/ml	Krebs-Henseleit Bicarbonate	D'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL. Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes , J Histochem Cytochem 45 , 965-73 , 1997
Rat Rat, SD, male	Epithelial	Collagenase : 50-75 u/ml Hyaluronidase : 0.1%	HBSS CF	Prasad, K.N. , Edwards-Prasad, J. , Carvaiho, I. , LaRosa, F.G. , Baibinder, E. , Meyers, A. and Quissell, D. Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments , In Vitro Cell Dev Biol 28 , 493 , 1992
Rat Rat, SD, albino, male, 150-200 g	Acinar, parotid	Trypsin : 0.01% Collagenase : 40-50 u/ml Hyaluronidase : 0.10%	HBSS CMF	Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T. Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) , Am J Physiol 229 (3) , 560 , 1975
Rat Rat, Wistar, male, 130 g	Acinar	Collagenase : 75 u/ml Hyaluronidase : 153 u/ml	RPMI 1640	Looms, D. , Dissing, S. , Tritsaris, K. , Pedersen, A. and Nauntofte, B. Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells. , Adv Dent Res 14 , 62 , 2000
Rat Rat, Wistar, male, 250 - 350 g	Acinar, parotid	Hyaluronidase : 0.015%	Earle's MEM	Melvin, J. , Kawaguchi, M. , Baum, B. and Turner, R. A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells , Biochem Biophys Res Commun 145 , 754 , 1987
Rat Rat, SD, male, 50 - 125 g	Acinar Exorbital lacrimal, parotid, pancreas	Trypsin : 0.01%	HBSS, CMF	Oliver, C. , Waters, J. , Tolbert, C. and Kleinman, H. Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel , In Vitro Cell Dev Biol 23 , 465 , 1987
Rat Rat, SD, male, 100-150 g	Parotid acinar	Trypsin : 0.001%	F12 medium	Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E. Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells , In Vitro Cell Dev Biol 27 , 707 , 1991
Rat Rat, Wistar, male	Parotid acinar	Trypsin : 0.02%	Solution B (See reference)	Foskett, J. , Roifman, C. and Wong, D. Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells , J Biol Chem 266 , 2778 , 1991