Brain

Species	Cells	Enzyme(s)	Medium	Reference
Bovine Bovine, adult	Cerebral artery Endothelial	Collagenase : 0.2%	HBSS	Machi, T. , Kassell, N.F. and Scheld, W.M. Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries , In Vitro Cell Dev Biol 26 , 291 , 1990
Bovine Calf (also lamb)	Oligodendroglia Neural	Trypsin : 0.1%	(see reference)	Poduslo, S. , Miller, K. and McKhann, G. Metabolic Properties of Maintained Oligodendroglia Purified from Brain , J Biol Chem 253 , 1592 , 1978
Bovine Bovine	Endothelial	Neutral Protease : 0.5%	MEM	Audus, K. and Borchardt, R. Characterization of an In Vitro Blood-Brain Barrier Model System for Studying Drug Transport and Metabolism , Pharm Res 3 (2) , 81 , 1986
Bovine Bovine	Endothelial	Neutral Protease : 0.125%	MEM	Miller, D. , Audus, K. and Borchardt, R. Application of Cultured Endothelial Cells of the Brain Microvasculature in the Study of the Blood-Brain Barrier , J Tiss Cul Meth 14 , 217 , 1992
Bovine Bovine	Capillary endothelial	Collagenase : 0.1%	DMEM	Estrada, C. , Bready, J. , Berliner, J. and Cancilla, P. Choline Uptake by Cerebral Capillary Endothelial Cells in Culture , J Neurochem 54 , 1467 , 1990
Bovine Bovine	Microvascular endothelial	Neutral Protease : 0.005%	Medium 199	Kanda, T. , Yoshino, H. , Ariga, T. , Yamawaki, M. and Yu, R. Glycosphingolipid Antigens in Cultured Microvasular Bovine Brain Endothelial Cells: Sulfoglucuronosyl Paragloboside as a Target of Monoclonal IgM in Demyelinative Neuropathy , J Cell Biol 126 (1) , 235 , 1994
Bovine Bovine	Brain endothelial cells	Collagenase Type 2 : 0.35%	DMEM	Wolburg H, Neuhaus J, Kniesel U, Krauss B, Schmid EM, Ocalan M, Farrell C, Risau W. Modulation of tight junction structure in blood-brain barrier endothelial cells. Effects of tissue culture, second messengers and cocultured astrocytes , J Cell Sci 107 , 1347 , 1994
Bovine Bovine	Endothelial Brain arteries	Collagenase Type 2 : 0.2%	Dulbecco's PBS	Goetz, I.. , Warren, J. , Estrada, C. , Roberts, E. and Krause, D. Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain , In Vitro Cell Dev Biol 21 , 172 , 1985
Guinea-Pig Guinea-pig, 200-400 g	Neurons	Trypsin : 0.06-0.08%	PIPES saline	Kay, A.R. and Wong, R.K.S. Isolation of Neurons Suitable for Patch-Clamping from Adult Mammalian Central Nervous Systems , J Neurosci Methods 16 , 227 , 1986
Human Human	Microvessels	Collagenase Type 4 : 0.1%	DMEM	Gerhart, D. Z. , Broderius, M. A. and Drewes, L. R. Cutlured Human and Canine Endothelial Cells from Brain Microvessels , Brain Res Bull 21 , 785 , 1988
Human Human, fetal	Astrocytes	PDS kit : see reference	EBSS	Barbar, L. , Rusielewicz, T. , Zimmer, M. , Kalpana, K. and Fossati, V. Isolation of Human CD49f Astrocytes and iPSC-Based Neurotoxicity Assays. , STAR Protoc Vol. 1 , 100172 , 2020
Human Human, 15-54 years	Microvessels	Collagenase : 0.1%	Serum-free modified Lewis medium	Vinters, H.V. , Reave, S. , Costello, P. , Girvin, J.P. and Moore, S.A. Isolation and Culture of Cells Derived From Human Cerebral Microvessels , Cell Tissue Res 249 , 657 , 1987
Human Human	Neuronal	Deoxyribonuclease I : 10 µg/ml	Tris-HCl, 50 mM, CaCl2, 2 mM	Roher, A.E. , Palmer, K.C. , Chau, V. and Ball, M.J. Isolation and Chemical Characterization of Alzheimer's Disease Paired Helical Filament Cytoskeletons:Differentiation from Amyloid Plaque Core Protein , J Cell Biol 107 , 2703 , 1988
Human Human	Microglia	Trypsin : 0.25% Deoxyribonuclease I : .005%	DMEM/F12	Klegeris Andis, McGeer PatrickL. Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity , Glia 51 , 56-64 , 2005
Human Human	Tumor	Collagenase Type 4 : 0.1% Hyaluronidase : 0.07% Deoxyribonuclease I : 0.04%	see reference	Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P. Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Human Human, fetal and mature	Astrocytes and neurons	Papain : 7.5-20 u/ml	RPMI	Zhang, Y. , Sloan, S. , Clarke, L. , Caneda, C. , Plaza, C. , Blumenthal, P. , Vogel, H. , Steinberg, G. , Edwards, M. , Li, G. , Duncan, J. , Cheshier, S. , Shuer, L. , Chang, E. , Grant, G. , Gephart, M. and Barres, B. Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. , Neuron 89 , 37-53 , 2016
Human Human	Microglia	Neutral Protease : 10 u/ml Papain : 2.5 u/ml	Hibernate A	Rustenhoven, J. , Park Thomas, I. , Schweder, P. , Scotter, J. , Correia, J. , Smith, A. , Gibbons, H. , Oldfield, R. , Bergin, P. , Mee, E. , Faull, R. , Curtis, M. , Scott, G. and Dragunow, M. Isolation of Highly Enriched Primary Human Microglia for Functional Studies. , Sci Rep 6 , 19371 , 2016
Human Human, 25-81 yr	Brain tumor	Neutral Protease : 0.11 u/ml Collagenase Type 4 : 0.05% Hyaluronidase : 1,000 u/ml Deoxyribonuclease I : 5 u/ml	HBSS	Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z, A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. , BMC Neurosci 17 , 30 , 2016
Human	Brain tumor	Collagenase Type 1 : 0.04% Hyaluronidase : 0.01% Deoxyribonuclease I : 0.02% Neutral Protease : 0.008%	DMEM/F12	Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J. Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. , Neuro Oncol 13 , 70-83 , 2011
Human Human	Microglia	Collagenase Type 1 : 300 u/ml Trypsin : 0.125%	DMEM	Mizee, M. , Miedema, S. , van der Poel, M. , Adelia , S. , van Strien, M. , Melief, J. , Smolders, J. , Hendrickx, D. , Heutinck, K. , Hamann, J. and Huitinga, I. Isolation of Primary Microglia from the Human Post-Mortem Brain: Effects of Ante- and Post-Mortem Variables. , Acta Neuropathol 5 , 16 , 2017
Human Human, adult	Neuronal	Papain : 20 u/ml	Neurobasal	Spaethling, J. , Na, Y. , Lee, J. , Ulyanova, A. , Baltuch, G. , Bell, T. , Brem, S. , Chen, H. , Dueck, H. , Fisher, S. , Garcia, M. , Khaladkar, M. , Kung, D. , Lucas, T. , O'Rourke, D. and Stefanik, D. Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics. , Cell Rep 18 , 791-803 , 2017
Human Human	Amyloid Fibrils	Collagenase Type 3 : 0.02% Deoxyribonuclease I : 0.001%	50mM Tris	Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S. Preparation of Amyloid Fibrils Seeded from Brain and Meninges , Methods Mol Biol 1345 , 299 , 2016
Insect Drosophila	Neurons	Papain : 20 u/ml	Saline	Gu, H. and O'Dowd, D. Whole Cell Recordings from Brain of Adult Drosophila. , J Vis Exp 6 , 248 , 2007
Insect Drosophila	Neurons	Papain : 4 u/ml	DMEM	Sicaeros, B. Campusano, J. and O'Dowd, D. Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae. , J Vis Exp 4 , 200 , 2007
Insect Drosophilia	Kenyon	Papain : 20 u/ml	Recording saline	Gu, H. and O'Dowd, D. Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ. , J Neurosci 26 , 265-72 , 2006
Insect Drosophilia	Neurons	Papain : 50 u/ml	Modified Dissecting Saline	Hadzic, T. , Park, D. , Abruzzi, K. , Yang, L. , Trigg, J. , Rohs, R. , Rosbash, M. and Taghert, P. Genome-Wide Features of Neuroendocrine Regulation in Drosophila by the Basic Helix-Loop-Helix Transcription Factor DIMMED. , Nucleic Acids Res 43 , 2199-215 , 2015
Monkey Monkey, rhesus	Brain cells	Collagenase Type 2 : 500 u/ml Deoxyribonuclease I : 28 u/ml	HBSS	Marcondes MC, Burudi EM, Huitron-Resendiz S, Sanchez-Alavez M, Watry D, Zandonatti M, Henriksen SJ, Fox HS. Highly activated CD8(+) T cells in the brain correlate with early central nervous system dysfunction in simian immunodeficiency virus infection , J Immunol 167 , 5429-38 , 2001
Mouse Mouse, 3 day	Microglia	Papain : 90 u/ml Deoxyribonuclease I : 2000 u/ml	Eagle's MEM	Nishioku T, Hashimoto K, Yamashita K, Liou SY, Kagamiishi Y, Maegawa H, Katsube N, Peters C, von Figura K, Saftig P, Katunuma N, Yamamoto K, Nakanishi H. Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia , J Biol Chem 277 , 4816 , 2002
Mouse Mouse, neonatal	Neurons	Papain : 20 u/ml	Neurobasal	Fasano, C. , Thibault, D. and Trudeau, L. Culture of Postnatal Mesencephalic Dopamine Neurons on an Astrocyte Monolayer , Current Protocols in Neuroscience Vol. 44 , 3.21.1 , 2008
Mouse Mouse, SD, 8-12 week	Microglia	Trypsin : 0.125% Collagenase Type 2 : 0.01% Deoxyribonuclease I : .005%	RPMI-1640	O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL. IGF-I and microglia/macrophage proliferation in the ischemic mouse brain , Glia 39 , 85 , 2002
Mouse Mouse, adult	Neurons	PDS kit : with modifications	DMEM/F12	Saxena, A. , Wagatsuma, A. , Noro, Y. , Kuji, T. , Asaka-Oba, A. , Watahiki, A. , Gurnot, C. , Fagiolini, M. , Hensch, T. and Carninci, P. Trehalose-Enhanced Isolation of Neuronal Sub-Types from Adult Mouse Brain. , Biotechniques 52 , 381 , 2012
Mouse Mouse	Cerebellar granule cell precursors	Papain : 0.435% Deoxyribonuclease I : 0.05%	EBSS	Miyazawa K, Himi T, Garcia V, Yamagishi H, Sato S. and Ishizaki Y. A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation , J Neurosci 20 , 5756 , 2000
Mouse Mouse	Brain-sequestered leukocytes	Collagenase Type 4 : 0.05% Deoxyribonuclease I : 2 u/ml	RPMI	Ryg-Cornejo, V. , Ioannidis, L. and Hansen, D. Isolation and Analysis of Brain-Sequestered Leukocytes from Plasmodium Berghei ANKA-Infected Mice. , J Vis Exp 71 , e50112 , 2013
Mouse Mouse	Neurosperes	PDS kit : per instructions	DMEM/F12	Klein C, Butt SJ, Machold RP, Johnson JE. and Fishell G. Cerebellum- and forebrain-derived stem cells possess intrinsic regional character , Development 132 , 4497 , 2005
Mouse Mouse	Cerebral pericytes	Collagenase Type 2 : 0.1% Deoxyribonuclease I : 30 u/ml	DMEM	Shah. G. , Price, T. , Banks, W. , Morofuji, Y. , Kovac, A. , Ercal, N. , Sorenson, C. , Shin, E. and Sheibani, N. Pharmacological Inhibition of Mitochondrial Carbonic Anhydrases Protects Mouse Cerebral Pericytes from High Glucose-Induced Oxidative Stress and Apoptosis. , J Pharmacol Exp Ther 344 , 637-45 , 2013
Mouse Mouse, newborn	Hippocampal cells	Papain : 10 u/ml	DMKM	Jun K, Choi G, Yang SG, Choi KY, Kim H, Chan GC, Storm DR, Albert C, Mayr GW, Lee CJ, Shin HS. Enhanced hippocampal CA1 LTP but normal spatial learning in inositol 1,4,5-trisphosphate 3-kinase(A)-deficient mice , Learn Mem 5 , 317-30 , 1998
Mouse Mouse, P13-15	Glial	Papain : 20 u/ml Deoxyribonuclease I : 0.0005%	EBSS	Haseleu, J. , Anlauf, E. , Blaess, S. , Endl, E. and Derouiche, A. Studying Subcellular Detail in Fixed Astrocytes: Dissociation of Morphologically Intact Glial Cells (DIMIGs). , Front Cell Neurosci 7 , 54 , 2013
Mouse Mouse, 2-5 day	Postnal substantia nigra	PDS kit : per instructions	see reference	Smeyne Michelle, Smeyne RichardJ. Method for culturing postnatal substantia nigra as an in vitro model of experimental Parkinson's disease , Brain Res Brain Res Protoc 9 , 105-11 , 2002
Mouse Mouse	Chloroid plexus	Collagenase Type 4 : 400 u/ml	PBS	Baruch, K. , Rosenzweig, N. , Kertser, A. , Deczkowska, A. , Sharif, A. , Spinrad, A. , Tsitsou-Kampeli, A. , Sarel, A. , Cahalon, L. and Schwartz, M. Breaking Immune Tolerance by Targeting Foxp3(+) Regulatory T Cells Mitigates Alzheimer's Disease Pathology. , Nat Commun 6 , 7967 , 2015
Mouse Mouse	Cortical neurons	PDS kit : per instructions	Neurobasal	Hernandez, F. , Perez, M. , Lucas, J. , Mata, A. , Bhat, R. and Avila, J. Glycogen Synthase Kinase-3 plays a Crucial Role in Tau Exon 10 Splicing and Intranuclear Distribution of SC35. Implications for Alzheimer's Disease. , J Biol Chem 279 , 3801 , 2004
Mouse Mouse, fetal and mature	Astrocytes and neurons	Papain : 7.5-20 u/ml	RPMI	Zhang, Y. , Sloan, S. , Clarke, L. , Caneda, C. , Plaza, C. , Blumenthal, P. , Vogel, H. , Steinberg, G. , Edwards, M. , Li, G. , Duncan, J. , Cheshier, S. , Shuer, L. , Chang, E. , Grant, G. , Gephart, M. and Barres, B. Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. , Neuron 89 , 37-53 , 2016
Mouse Mouse, 1 day	Neurons and glia	PDS kit : per instructions	Neurobasal	Martin-Aparicio, E. , Yamamoto, A. , Hernandez, F. , Hen, R. , Avila, J. and Lucas, J. Proteasomal-Dependent Aggregate Reversal and Absence of Cell Death in a Conditional Mouse Model of Huntington's Disease. , J Neurosci 21 , 8772 , 2001
Mouse Mouse, 3 week	Neural	Papain : 20 u/ml	PBS	Luo,Y.,Coskun, V. , Liang, A.,Yu, J. , Cheng, L.,Ge, W. , Shi, Z. , Zhang, K. , Li, C. , Lin, H. , Luo, D. , Wang, J. , Lin, C. , Dai, Z. , Zhu, H. , Zhang, J. , Liu, J. , Liu, H.,deVellis, J. , Horvath, S. , Sun, Y. and Li, S. Different Patterns of Molecular Forms of Somatostatin are Released by the Rat Median Eminence and Hypothalamus. , Cell 161 , 1175 , 2015
Mouse Mouse, 1 day	Neural progenitor	PDS kit : per instructions	see reference	Seaberg, R. , Smukler, S. and Van der Kooy, D. Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain. , Dev Biol 278 , 71 , 2005
Mouse Mouse, P0-P15	Astrocytes	Papain : 5 u/ml Deoxyribonuclease I : 0.004%	DPBS	Foo, L. Purification of Rat and Mouse Astrocytes by Immunopanning. , Cold Spring Harb. Protoc. 2013 , 421-32 , 2013
Mouse Mouse, embryonic	Cortical progenitors	PDS kit : per instructions	Serum free medium	Estivill-Torrus, G. , Pearson, H. , Van Heyningen, V. , Price, D. and Rashbass, P. Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors. , Development 129 , 455 , 2002
Mouse Mouse, adult	Astrocytes, microglia, endothelia, oligodendrocytes	Papain : 20 u/ml Collagenase Type 1 : 0.01%	HBSS	Swartzlander, D. , Propson, N. , Roy, E. , Saito, T. , Saido, T. , Wang, B. and Zheng, H. Concurrent Cell Type-Specific Isolation and Profiling of Mouse Brains in Inflammation and Alzheimer's Disease. , JCI Insight 3 , 2018
Mouse Mouse, postnatal	Astrocytes	Trypsin : 0.25% Deoxyribonuclease I : 1,000 u/ml	HBSS	Sher, F. , Rossler, R. , Brouwer, N. , Balasubramaniyan, V. , Boddeke, E. and Copray, S. Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2. , Stem Cells 26 , 2875 , 2008
Mouse Adult and neonatal	Microglia, astrocytes, lymphocytes	Collagenase Type 2 : 120 u/ml Papain : 20 u/ml Neutral Protease : 1.2 u/ml Deoxyribonuclease I : 20 u/ml	EBSS	Calvo, Belen, Rubio, F. , Fernandez, M. and Tranque, P. Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry. , IBRO Rep Vol. 8 , 36-47 , 2020
Mouse Mouse	Granule cell precursors, pre-neoplastic and tumor cells	Papain : 10 u/ml Deoxyribonuclease I : 250 u/ml	Neurobasal/B27	Oliver, T. , Read, T. , Kessler, J. , Mehmeti, A. , Wells, J. , Huynh, T. , Lin, S. and Wechsler-Reya, R. Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma. , Development 132 , 2425 , 2005
Mouse Mouse	Neurons	Papain : 10 u/ml Deoxyribonuclease I : 10 u/ml	Artificial CSF	Kramer, D. , Risso, D. , Kosillo,. , Ngai, J. and Bateup, H. Combinatorial Expression of Grp and Neurod6 Defines Dopamine Neuron Populations with Distinct Projection Patterns and Disease Vulnerability , eNeuro Vol. 5 , 1 , 2018
Mouse Mouse, 6-12 week	Vascular smooth muscle cells	Papain : 0.05% Collagenase Type 4 : 0.15% Elastase : 0.05%	PBS	Chung, W. , Farley, J. , Swenson, A. , Barnard, J. , Hamilton, G. , Chiposi, R. and Drummond, H. Extracellular Acidosis Activates ASIC-like Channels in Freshly Isolated Cerebral Artery Smooth Muscle Cells. , Am J Physiol Cell Physiol 298 , C1198 , 2010
Mouse Mouse, SWR or CF1, 1-3 months	Papillae, taste receptor	Pronase E : 0.15%	Carbonate-Phosphate buffer (see reference)	Spielman, A. , Mody, I. , Brand, J. , Whitney, G. , MacDonald, J. and Salter, M. A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells , Brain Res 503 , 326 , 1989
Mouse Mouse, 1-2 day	Oligodendrocytes, dorsal root ganglia	Papain : 0.15% Deoxyribonuclease I : 0.006%	DMEM	O'Meara, R. , Ryan, S. , Colognato, H. and Kothary, R. Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues. , J Vis Exp 54 , 3324 , 2011
Mouse Mouse, 0-30 day	Neural	Trypsin NF 1:250 : 0.25%	BSS	Shrier, B. , Wilson, S. and Nirenberg, M. Cultured Cell Systems and Methods for Neurobiology , Vol. 32 , 765 , 1974
Mouse Mouse, 4-6 day	Neurons	PDS kit : with modifications	HBSS	Lee, H. , Greene, L. , Mason, C. and Manzini, M. Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons. , J Vis Exp 23 , 990 , 2009
Mouse Mouse	Astrocytes	Trypsin : 0.25%	DMEM	Lim, D. and Alvarez-Buylla, A. Interaction Between Astrocytes and Adult Subventricular Zone Precursors Stimulates Neurogenesis , Proc Natl Acad Sci U S A 96 , 7526 , 1999
Mouse Mouse, embryonic and postnatal	Cortical neurons	Papain : 4-10 u/ml	Neurobasal	Hilgenberg, L. and Smith, M. Preparation of Dissociated Mouse Cortical Neuron Cultures. , J Vis Exp 10 , 562 , 2007
Mouse Mouse, adult	Interneurons	PDS kit	EBSS	Joseph, D. , Deimling, M. , Hasegawa, Y. , Cristancho, A. , Risbud, R. , McCoy, A. and Marsh, E. Protocol for Isolating Young Adult Parvalbumin Interneurons from the Mouse Brain for Extraction of High-Quality RNA. , STAR Protoc Vol. 2 , 100714 , 2021
Ovine Lamb (also calf)	Oligodendroglia Neural	Trypsin : 0.1%	(see reference)	Poduslo, S. , Miller, K. and McKhann, G. Metabolic Properties of Maintained Oligodendroglia Purified from Brain , J Biol Chem 253 , 1592 , 1978
Ovine Ovine	Neurons	PDS kit : per instructions	DMEM	Lepore G, Gadau S, Mura A, Zedda M, Farina V. Aromatase Immunoreactivity in Fetal Ovine Neuronal Cell Cultures Exposed to Oxidative Injury. , Eur J Histochem 53 , e28 , 2009
Ovine Ovine	Neurons	Papain : 0.2% Deoxyribonuclease I : 0.1%	Hibernate	Reddy, R. , Amodei, R. , Estill, C. , Stormshak, F. , Meaker, M. and Roselli, C. Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture. , PLoS ONE 10 , e0129521 , 2015
Porcine Mini pigs, Yucatan (Susscrofa Yucatan), 4-6 months	Microvascular	Collagenase : 0.1%	HBSS	Robinson, D.H. , Kang,Y. , Deschner, S.H. and Nielsen, T.B. Morphologic Plasticity and Periodicity : Porcine Cerebral Microvascular Cells in Culture , In Vitro Cell Dev Biol 26 , 169 , 1990
Rat Rat, SD, E16	Cortical neurons	PDS kit : with modifications	Neurobasal Medium	Varney MA, Cosford ND, Jachec C, Rao SP, Sacaan A, Lin FF, Bleicher L, Santori EM, Flor PJ, Allgeier H, Gasparini F, Kuhn R, Hess SD, Velicelebi G, Johnson EC. SIB-1757 and SIB-1893: selective, noncompetitive antagonists of metabotropic glutamate receptor type 5 , J Pharmacol Exp Ther 290 , 170 , 1999
Rat Rat, Fisher, 7-21 month	Hippocampal neurons	Papain : 0.2%	Hibernate A	Chen, N. , Newcomb, J. , Garbuzova-Davis, S. , Davis Sanberg, C. , Sanberg, P. and Willing, A. Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro. , Aging Dis 1 , 173 , 2010
Rat Rat, albino, adult and newborn	Cerebral cortices	Trypsin : 0.25%	PBS	Giulian, D. and Baker, T.J. Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain , J Neurosci 6 , 2163 , 1986
Rat Rat, Wistar, 14 day	Visual cortical	PDS kit : per instructions	EBSS	Mizoguchi Y, Kanematsu T, Hirata M, Nabekura J. A rapid increase in the total number of cell surface functional GABAA receptors induced by brain-derived neurotrophic factor in rat visual cortex , J Biol Chem 278 , 44097 , 2003
Rat Rat, neonatal	Dura mater	Collagenase Type 1 : 0.075%	DMEM	Peptan, I. , Hong, L. and Evans, C. Multiple Differentiation Potentials of Neonatal Dura Mater-Derived Cells. , Neurosurgery 60 , 346 , 2007
Rat Rat, Wistar, newborn	Germinal matrix	Trypsin : 0.25%	HBSS	Goldman, J.E. , Geier, S.S. and Hirano, M. Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture , J Neurosci 6 , 52 , 1986
Rat Rat, Wistar, male	Cerebral endothelial	Collagenase Type 3 : 0.2%	MEM	Floris S, Van den Born J, van der Pol SM, Dijkstra CD, De Vries HE. Heparan sulfate proteoglycans modulate monocyte migration across cerebral endothelium , J Neuropathol Exp Neurol 62 , 780 , 2003
Rat Rat, SD	Hippocampal	Trypsin : 0.1%	Neurobasal	Akanda, N. , Tofighi, R. , Brask, J. , Tamm, C. , Elinder, F. and Ceccatelli, S. Voltage-Dependent Anion Channels (VDAC) in the Plasma Membrane Play a Critical Role in Apoptosis in Differentiated Hippocampal Neurons but not in Neural Stem Cells. , Cell Cycle 7 , 3225 , 2008
Rat Rat, Wistar-Kyoto, male, 100 - 200 g	Endothelial Cerebral	Collagenase Type 2 : 0.05%	HBSS	Diglio, C.A. , Grammas, P. , Filiberto Giacomelli, M.S. and Wiener, J. Primary Culture of Rat Cerebral Microvascular Endothelial Cells , Lab Invest 46 , 554 , 1982
Rat Rat, wistar, neonatal	Hippocampal cells	Papain : 1 mg/ml	DMEM	Boehm S, Betz H. Somatostatin inhibits excitatory transmission at rat hippocampal synapses via presynaptic receptors , J Neurosci 17 , 4066 , 1997
Rat Rat, SD, 1-2 day	Oligodendrocyte precursor cells	Papain : 16.5 u/ml	HBSS	Kurachi, M. , Mikuni, M. and Ishizaki, Y. Extracellular Vesicles from Vascular Endothelial Cells Promote Survival, Proliferation and Motility of Oligodendrocyte Precursor Cells. , PLoS ONE 11 , e0159158 , 2016
Rat Rat, 60-72 hours old	Fibroblasts	Trypsin : 0.2%	HEPES buffered DMEM	Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R. Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF , Neuron 7 , 265 , 1991
Rat Rat, wistar, 250-300 g	Cerebral artery smooth muscle cells	Papain : 1.5 mg/ml Collagenase Type 4 : 1.5 mg/ml	Physiological Salt Solution	Brzezinska AK, Gebremedhin D, Chilian WM, Kalyanaraman B, Elliott SJ. Peroxynitrite reversibly inhibits Ca(2+)-activated K(+) channels in rat cerebral artery smooth muscle cells , Am J Physiol Heart Circ Physiol 278 , H1883 , 2000
Rat Rat, P0-P15	Astrocytes	Papain : 5 u/ml Deoxyribonuclease I : 0.004%	DPBS	Foo, L. Purification of Rat and Mouse Astrocytes by Immunopanning. , Cold Spring Harb. Protoc. 2013 , 421-32 , 2013
Rat Rat, Wistar, adult, 170 g	Endothelial	Trypsin : 0.5%	BSS	Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M. Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter , J Anat 129 , 261 , 1979
Rat Rat, SD, Fisher	Hippocampal neurons	Papain : 0.2%	HibernateA/B27	Brewer, G.J. Isolation and culture of adult rat hippocampal neurons of any age , J Neurosci Methods 71 , 143 , 1997
Rat Rat, SD, E18	Neurons	Papain : 20 u/ml	HBSS	Folts, C. , Scott-Hewitt, N. , Proschel, C. , Mayer-Proschel, M. and Noble, M. Lysosomal Re-acidification Prevents Lysosphingolipid-Induced Lysosomal Impairment and Cellular Toxicity. , PLoS Biol 14 , e1002583 , 2016
Rat Rat, Wistar, pregnant	Glial	Trypsin : 0.05%	Eagle's MEM/DMEM	Abney, E. , Bartlett, P. and Raff, M. Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain , Dev Biol 83 , 301 , 1981
Rat Rat, E18	Hippocampal and cortical neurons	PDS kit : per instructions	Neurobasal media	Naeve GS, Ramakrishnan M, Kramer R, Hevroni D, Citri Y, Theill LE. Neuritin: a gene induced by neural activity and neurotrophins that promotes neuritogenesis , Proc Natl Acad Sci U S A 94 , 2648-53 , 1997
Rat Rat, SD, neonatal	Neurons	Papain : 20 u/ml	Neuro medium	Kaiser, O. , Aliuos, P. , Wissel, K. , Lenarz, T. , Werner, D. , Reuter, G. , Kral, A. and Warnecke, A. Dissociated Neurons and Glial Cells Derived from Rat Inferior Colliculi After Digestion with Papain. , PLoS ONE 8 , e80490 , 2013
Rat Rat, SD, adult	Endothelial	Collagenase Type 2 : 0.5%	Medium 199	Doron, D. , Jacobowitz, D. , Heldman, E. , Feurerstein, G. , Pollard, H. and Hallenbeck, J. Extracellular Matrix Permits the Expression of Von Willebrand's Factor, Uptake of Di-I-Acetylated Low Density Lipoprotein and Secretion of Prostacyclin in Cultures of Endothelial Cells from Rat Brain Microvessels , In Vitro Cell Dev Biol 27A , 689 , 1991
Rat Rat, SD, E18	Cortical cells	Papain : 20 u/ml Deoxyribonuclease I : .005%	EBSS	Behar, T, Smith, S, Kennedy, R, Mckenzie, J, Maric, I. and Barker, J GABA(B) receptors mediate motility signals for migrating embryonic cortical cells , Cereb Cortex 11 , 744-53 , 2001
Rat Rat, SD, female	Fetal rat brain	Collagenase Type 4 : 0.1%	DMEM	Matsuda, M. Serum Proteins Enhance Aggregate Formation of Dissociated Fetal Rat Brain Cells in an Aggregating Culture , In Vitro Cell Dev Biol 24 (10) , 1031 , 1988
Rat Rat, E18	Hippocampal neurons	Papain : 0.2%	Hibernate	Jekabsons MB, Nicholls DG. Bioenergetic analysis of cerebellar granule neurons undergoing apoptosis by potassium/serum deprivation , Cell Death Differ 13 , 1595-610 , 2006
Rat Rat, Wistar, male, 300-500 g	Microvessels Endothelial	Collagenase Type 2 : 0.75%	Ringers-HEPES buffer	Williams, S. , Gillis, J. , Matthews, M. , Wagner, R. and Bitensky, M. Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity , J Neurochem 35 (2) , 374 , 1980
Rat Rat, SD, one month old	Capillary endothelium Pericytes	Neutral Protease : 0.1%	Medium 199	Bowman, P. , Betz, A. , Ar, D. , Wolinsky, J. , Penney, J. , Shivers, R. and Goldstein, G. Primary Culture of Capillary Endothelium From Rat Brain , In Vitro 17 (4) , 353 , 1981
Rat Rat, E19	Hippocampal neuron	Papain : 10 u/ml	MEM	Khakh Baljit S, Fisher James A, Nashmi Raad, Bowser David N, Lester Henry A. An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy , J Neurosci 25 , 6911-20 , 2005
Rat Rat, newborn	Astrocytes	Trypsin : 0.25%	DMEM, HBSS	Holzwarth, J. , Glaum, S. and Miller, R. Activation of Endothelin Receptors by Sarafotoxin Regulates Ca2+ Homeostasis in Cerebellar Astrocytes , Glia 5 , 239 , 1992
Rat Rat, neonatal	Hippocampal neurons	Papain : 10 u/ml	EBSS	Richler Esther, Chaumont Severine, Shigetomi Eiji, Sagasti Alvaro, Khakh Baljit S. Tracking transmitter-gated P2X cation channel activation in vitro and in vivo , Nat Methods 5 , 87-93 , 2008
Rat Rat, Holtzmann, 18 day	Hippocampal neurons	Trypsin : 0.1%	HBSS	Banker, G. and Cowan, M. Rat Hippocampal Neurons in Dispersed Cell Culture , Brain Res 126 , 397 , 1977
Rat Rat, Wistar, 1-3 day	Hippocampal neurons	Trypsin : 0.05%	DMEM	Velasco Myrian, Garcia Esperanza, Onetti Carlos G. Glucose deprivation activates diversity of potassium channels in cultured rat hippocampal neurons , Cell Mol Neurobiol 26 , 307-19 , 2006
Rat Rat, fetus, 18 day	Hippocampi	Trypsin : 0.1%	HBSS	Rothman, S. Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death , J Neurosci 4 (7) , 1884 , 1984
Rat Rat, SD, 5 day	Hippocampal neurons	Trypsin : 0.2% Deoxyribonuclease I : 10 ug/ml	HBSS	Mattson, M. and Kater, S. Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions , Brain Res 490 , 110 , 1989
Rat Rat, SD, 19-20 days pregnant	Neural	Trypsin : 0.25%	DMEM	Ahmed, Z. , Walker, P. and Fellows, R. Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture , J Neurosci 3 (12) , 2448 , 1983
Rat Rat, SD, 7 day	Cerebellar granule neurons	PDS kit : per instructions	PBS	Tanaka, S. , Shaikh, I. , Chiocca, E. and Saeki, Y. The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development. , PLoS ONE 4 , e5922 , 2009
Rat Rat, SD, fetal	Cerebral cortex Hypothalmus	Deoxyribonuclease I : 0.001%	HEPES	Peterfreund, R. and Vale, W. High Molecular Weight Somatostatin Secretion by Cultured Rat Brain Cell , Brain Res 239 , 463 , 1982
Rat Rat, fetus	Cortical	Trypsin : 0.25%	Puck's D1 (see reference)	Swaiman, K. , Neale, E. , Fitzgerald, S. and Nelson, P. A Method for Large-scale Production of Mouse Brain Cortical Cultures , Brain Res 255 , 361 , 1982
Rat Rat, SD, 19-21 day	Suprachiasmatic nucleus neurons	Papain : 100 u/ml	MEM	Cao, R. , Li, A. , Cho, H. , Lee, B. and Obrietan, K. Mammalian Target of Rapamycin Signaling Modulates Photic Entrainment of the Suprachiasmatic Circadian Clock. , J Neurosci 30 , 6302 , 2010
Rat Rat, SD, newborn	Glial	Trypsin : 0.0625%	MEM, sterile	Pixley, S.K. The Olfactory Nerve Contains Two Populations of Glia , In Vitro 5 , 269 , 1992
Rat Rat, SD, 2 days	Pineal	Trypsin : 0.25%	DMEM, MEM	Rowe, V. , Neale, E. , Avins, L. , Guroff, G. and Schrier, B. Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons , Exp Cell Res 104 , 345 , 1977
Rat Rat, neonatal	Astrocytes	Papain : 20 u/ml	EBSS	Shigetomi, E. and Khakh, B. Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes. , J Vis Exp 26 , 1142 , 2009
Rat Rat, fetus, 18-20 day	Hippocampal neurons	Trypsin : 0.25%	HBSS, CMF	Bartlett, W. and Banker, G. An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts , J Neurosci 4 , 1944 , 1984
Shellfish Helisoma trivolvis	Neurons Buccal ganglia	Trypsin : 0.2%	Saline, Sterile	Hadley, R.D. , Bodnar, D.A. and Kater, S.B. Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation , J Neurosci 5 , 3145 , 1985