Pituitary

Species	Cells	Enzyme(s)	Medium	Reference
Bovine Bovine	Pituitary	Collagenase : 0.1%	EBSS, CMF	Mason, W. and Ingram, C. Techniques for Studying the Role of Electrical Activity in Control of Secretion by Normal Anterior Pituitary Cells , Vol. 124 , 207 , 1986
Bovine Bovine	Follicular, anterior pituitary and pars tuberalis	Deoxyribonuclease I : 200 µg/ml	HBSS, CMF	Ferrara, N. , Goldsmith, P. , Fujii, D. and Weiner, R. Culture and Characterization of Follicular Cells of the Bovine Anterior Pituitary and Pars Tuberalis , Vol. 124 , 245 , 1986
Bovine Calf, male, less then 6 months old	Pituitary	Collagenase : 0.3%	DMEM	Hassan, H. and Merkel, R. Perifusion Model System to Culture Bovine Hypothalamic Slices In Series with Dispersed Anterior Pituitary Cells , In Vitro Cell Dev Biol 30A , 435 , 1994
Bovine Calf, male, 1-6 week old	Pitituary	Hyaluronidase : 0.1%	DMEM	Ridgway, E. , Klibanski, A. , Marorana, M. , Milbury, P. , Kieffer, J. and Chin, W. The Effect of Somatostatin on the Release of Thyrotropin and its Subunits from Bovine Anterior Pitituary Cells in Vitro , Endocrinology 112 (6) , 1937 , 1983
Mouse Mouse	Pituitary	Collagenase Type 2 : 0.5% Deoxyribonuclease I : 0.005%	HBSS	Perez Millain, M. , Brinkmeier, M. , Mortensen, A. and Camper, S. PROP1 Triggers Epithelial-Mesenchymal Transition-Like Process in Pituitary Stem Cells. , Elife 5 , 2016
Mouse Mouse	Pituitary	Collagenase Type 1 : 1,000 u/ml Soybean Trypsin Inhibitor : 0.01% Hyaluronidase : 0.1% Deoxyribonuclease I : 0.001%	DMEM	Pyczek, J. , Buslei, R. , Schult, D. , Holsken, A. , Buchfelder, M. , Hess, I. , Hahn, H. and Uhmann, A. Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland. , Sci Rep 6 , 24928 , 2016
Mouse Mouse, male	Pituitary	Collagenase : 0.4% Hyaluronidase : 0.1% Trypsin : 0.3%	DMEM/Han's F12	Steveson Tami C, Ciccotosto Giuseppe D, Ma Xin-Ming, Mueller Gregory P, Mains Richard E, Eipper Betty A. Menkes protein contributes to the function of peptidylglycine alpha-amidating monooxygenase , Endocrinology 144 , 188-200 , 2003
Ovine Ovine, adult	Somatotropes	Collagenase Type 1 : 0.3% Hyaluronidase	Medium 199	Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen. Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes , Endocrinology 143 , 4609-19 , 2002
Rat Rat, SD, female, 200 - 250 g	Anterior pituitary gland	Trypsin : 0.1%	EBSS, CMF	D'Emden, M. and Wark, J. Culture Requirements for Optimal Expression of 1,25-Dihydroxyvitamin D3-Enhanced Thyrotropin Secretion , In Vitro Cell Dev Biol 27 , 197 , 1991
Rat Rat, Wistar, male, 2 month old	Pituitary	Trypsin : 0.1%	DMEM	Zhou, X. , De Schepper, J. , De Craemer, D. , Delhase, M. , Gys, G. , Smitz, J. and Hooghe-Peters, E. Pituitary Growth Hormone Release and Gene Expression in Cafeteria-Diet-Induced Obese Rats , J Endocrinol 159 , 165 , 1998
Rat Rat, SD, male, 250-450 g	Anterior pituitary	Trypsin : 0.25%	Krebs	Portanova, R. , Smith, D. and Sayers, G. A Trypsin for the Preparation of Isolated Rat Anterior Pituitary , Proc Soc Exp Biol Med 133 , 573 , 1970
Rat Rat	Pituitary	Trypsin : 0.3 %	HEPES	Wilfinger, W. , Larsen, W. , Downs, T. and Wilbur, D. An In Vitro Model for Studies of Intercellular Communication in Cultured Rat Anterior Pituitary Cells , Tissue Cell 16 (4) , 483 , 1984
Rat Rat, male, 12-15 week	Pituitary	Collagenase Type 2 : 0.4% Deoxyribonuclease I : 0.04%	DMEM	Akieda-Asai, S. , Zaima, N. , Ikegami, K. , Kahyo, T, Yao, I. , Hatanaka, T. , Iemura, S. , Sugiyama, R. , Yokozeki, T. , Eishi, Y. , Koike, M. , Ikeda, K. , Chiba, T. , Yamaza, H. , Shimokawa, I. , Song, S. , Matsuno, A. , Mizutani, A. , Sawabe, M. Chao, N. and Tanaka, M. SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5K gamma Activity through Deacetylation of Specific Lysine Residues in Mammals. , PLoS ONE 5 , e11755 , 2010