Miscellaneous

Species	Cells	Enzyme(s)	Medium	Reference
Bovine Bovine	Nucleus pulposus	Collagenase Type 2 : 0.2%	DMEM	Lee, J. , Cheung, K. and Leung, V. Systematic Study of Cell Isolation from Bovine Nucleus Pulposus: Improving Cell Yield and Experiment Reliability. , J Orthop Res 33 , 1743-55 , 2015
Equine Equine	Tendon Cells	Collagenase Type 1 : 0.1%	DMEM	Nemoto, M. , Kizaki, K. , Yamamoto, Y. , Oonuma, T. and Hashizume, K. Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration. , J Equine Sci 24 , 17-24 , 2013
Goat Goat	Infrapatellar fat pad mesenchymal stromal	Collagenase : 0.15%	DMEM	Arora, A. , Sriram, M. , Kothari, A. and Katti, D. Co-culture of Infrapatellar Fat Pad-Derived Mesenchymal Stromal Cells and Articular Chondrocytes in Plasma Clot for Cartilage Tissue Engineering. , Cytotherapy 19 , 881-894 , 2017
Human Human	Esophageal microvascular endothelial	Collagenase Type 2 : 0.2%	MCDB-131	Rafiee, P. , Ogawa, H. , Heidemann, J. , Li, M. , Aslam, M. , Lamirand, T. , Fisher, P. , Graewin, S. , Dwinell, M. , Johnson, C. , Shaker, R. and Binion, D. Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation. , Am J Physiol Gastrointest Liver Physiol 285 , G1277 , 2003
Human Human	Fungiform taste papillae	Collagenase Type 1 : 550 u/ml Elastase : 10 u/ml Soybean Trypsin Inhibitor : 0.09%	see reference	Ozdener, H. , Spielman, A. and Rawson, N. Isolation and Culture of Human Fungiform Taste Papillae Cells , J Vis Exp 63 , e3730 , 2012
Human Human	Adipose derived stromal	Collagenase Type 2 : 0.075%	DMEM	Panetta, N. , Gupta, D. , Kwan, M. , Wan, D. , Commons, G. and Longaker, M. Tissue Harvest by Means of Suction-Assisted or Third-Generation Ultrasound-Assisted Lipoaspiration Has No Effect on Osteogenic Potential of Human Adipose-Derived Stromal Cells. , Plast Reconstr Surg 124 , 65 , 2009
Human Human, 3-7 year	Dental pulp stem cells	Collagenase Type 1 : 0.3% Neutral Protease : 0.4%	DMEM	Salmon, B. , Bardet, C, Khaddam, M. Naji, J. , Coyac, B. , Baroukh, B. , Letourneur, F. , Lesieur, J. , Decup, F. , Le Denmat, D. , Nicoletti, A. , Poliard, A, Rowe, P. , Huet, E. , Vital, S. and Linglart, A. MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia. , PLoS ONE 8 , e56749 , 2013
Human Human	Dental pulp stem	Collagenase Type 1 : 0.3% Neutral Protease : 0.4%	DMEM/F12	Bonnamain, V. , Thinard, R. , Sergent-Tanguy, S. , Huet, P. , Bienvenu, G. , Naveilhan, P. , Farges, J. and Alliot-Licht, B. Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium. , Front Physiol 4 , 357 , 2013
Human Human	Oral epithelial	Neutral Protease : 1.2 u/ml Trypsin : 0.05%	PBS	Nakamura, T. , Endo, K. and Kinoshita, S. Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling. , Stem Cells 25 , 628-38 , 2007
Human Human	Tendon cells	Collagenase Type 2 : 0,3%	DMEM/Hams F12	Pauly, S. , Klatte, F. , Strobel, C. , Schmidmaier, G. , Greiner, S. and Scheibel, M. Wildemann B Characterization of Tendon Cell Cultures of the Human Rotator Cuff. , Eur Cell Mater 20 , 84-97 , 2010
Human Human	Mesenchymal stromal	Collagenase Type 1 : 0.075%	PBS	Lee, Y. , Lee, J. , Park, H. , Lim, Y. , Lee, J. , Wang, S. and Lee, B. Isolation of Mesenchymal Stromal Cells (MSCs) from Human Adenoid Tissue. , Cell Physiol Biochem 31 , 513-24 , 2013
Human Human	Nucleus pulposus	Pronase : 0.25% Collagenase Type 2 : 600 u/ml	DMEM	Sun, Y. , Lv, M. , Zhou, L. , Tam, V. , Lv, F. , Chan, D. , Wang, H. , Zheng, Z. , Cheung, K. and Leung, V. Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation. , Osteoarthritis Cartilage 23 , 1194-203 , 2015
Human Human	Adhesion fibroblasts	Collagenase Type 4 : 0.2%	Medium 199	Foster, D. , Marshall, C. , Gulati, G. , Chinta, M. , Nguyen, A. , Salhotra, A. , Jones, R. , Burcham, A. , Lerbs, T. , Cui, L. , King, M. , Titan, A. , Ransom, R. , Manjunath, A. , Hu, M. and Blackshear, C. Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation. , Nat Commun 11 , 4061 , 2020
Human Human	Cortical spheroids	Papain : 40 u/ml	EBSS	Yoon, S. , Elahi, L. , Pașca, A. , Marton, R. , Gordon, A. , Revah, O. , Miura, Y. , Walczak, E. , Holdgate, G. , Fan, H. , Huguenard, J. , Geschwind, D. and Pașca, S. Reliability of Human Cortical Organoid Generation. , Nat Methods 16 , 75-78 , 2019
Human Human	Stem cell-derived retinal organoids	PDS kit : see reference	EBSS	Xue, Y. , Seiler, M. , Tang, W. , Wang, J. , Delgado, J. , McLelland, B. , Nistor, G. , Keirstead, H. and Browne, A. Retinal Organoids On-a-Chip: a Micro-Millifluidic Bioreactor for Long-term Organoid Maintenance. , Lab Chip 21 , 3361-3377 , 2021
Human Human	Periapical granuloma	CLSPA : 0.25%	RPMI-1640	Stern MH, Dreizen S, Mackler BF, Levy BM. Isolation and characterization of inflammatory cells from the human periapical granuloma , J Dent Res 61 , 1408-12 , 1982
Human Human	Carotid artery plaque macrophage	Collagenase Type 4 : 450 u/ml Deoxyribonuclease I : 500 u/ml Soybean Trypsin Inhibitor : 0.1%	HBSS	Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM. Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin , Circ Res 98 , 1282-9 , 2006
Human Human	Brain organoids	PDS kit : see reference	PBS	Velasco, S. , Kedaigle, A. , Simmons, S. , Nash, A. , Rocha, M. , Quadrato, G. , Paulsen, B. , Nguyen, L. , Adiconis, X. , Regev, A. , Levin, J. and Arlotta, P. Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. , Nature 570 , 523-527 , 2019
Human Human	Synoviocytes	Collagenase : 0.2%	DMEM/F12	Chen V, Croft D, Purkis P, Kramer IM. Co-culture of synovial fibroblasts and differentiated U937 cells is sufficient for high interleukin-6 but not interleukin-1beta or tumour necrosis factor-alpha release. , Br J Rheumatol 37 , 148-56 , 1998
Human	Gastric epithelial cells	Collagenase Type 2 : 200 u/ml Neutral Protease : 1.2 u/ml Soybean Trypsin Inhibitor : 0.125%	L-15	Smoot, D. , Sewchand, J. , Young, K. , Desbrodes, B. , Allen, C. and Naab, T. A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells , Meth Cell Sci 22 , 133 , 2000
Human Human	Peptic cells	Collagenase Type 4 : 0.1% Soybean Trypsin Inhibitor : 0.2%	Ringer solution	Lanas. A. , Nerin, J. , Esteva, F. and Sainz, R. Non-Steroidal Anti-Inflammatory Drugs and Prostaglandin Effects on Pepsinogen Secretion by Dispersed Human Peptic Cells. , Gut 36 , 657-63 , 1995
Insect Insect, Lepidoptera	Lepitopteran	Collagenase Type 3 : 0.35% Hyaluronidase : 0.01%	Dulbecco PBS	Goodwin, R. and McCawley, P Initiating Attached Cell Lines From the Lepidoptera (Insecta) , Meth Cell Sci 3 , 567 , 1977
Invertebrate Squid	Mantle	Collagenase : see reference Papain : see reference	Phosphate buffer	Raman, M. and Mathew, S. Study of Chemical Properties and Evaluation of Collagen in Mantle, Epidermal Connective Tissue and Tentacle of Indian Squid, Loligo duvauceli Orbigny. , J Food Sci Technol 51 , 1509-16 , 2014
Invertebrate Octopus vulgaris	Neurons	Papain : 0.1% Collagenase Type 4 : 0.4% Trypsin : 0.1%	L15-ASW	Maselli, V. , Xu, F. , Syed, N. , Polese, G. and Cosmo, A. A Novel Approach to Primary Cell Culture for Octopus vulgaris Neurons. , Front Physiol 9 , 220 , 2018
Mouse Mouse	Cochleaer	Trypsin : 0.125%	DMEM/F-12	Jan, T. , Chai, R. , Sayyid, Z. and Cheng, A. Isolating LacZ-Expressing Cells from Mouse Inner Ear Tissues Using Flow Cytometry. , J Vis Exp 58 , e3432 , 2011
Mouse Mouse, 3-6 week	Ear mesenchymal stem	Collagenase Type 1 : 0.2%	DMEM/F12	Staszkiewicz, J. , Gimble, J. , Manuel, J. and Gawronska-Kozak, B. IFATS Collection: Stem Cell Antigen-1-Positive Ear Mesenchymal Stem Cells Display Enhanced Adipogenic Potential. , Stem Cells 26 , 2666 , 2008
Mouse Mouse, 26-30 g	Myenteric plexus	Collagenase Type 2 : 0.13%	Neurobasal A	Smith, T. , Grider, J. , Dewey, W. and Akbarali, H. Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels. , PLoS ONE 7 , e45251 , 2012
Mouse Mouse, 10 week	Synovial mesenchymal	Collagenase : 0.1% Deoxyribonuclease I : 0.005%	DMEM	Futami, I. , Ishijima, M. , Kaneko, H. , Tsuji, K. , Ichikawa-Tomikawa, N. , Sadatsuki, R. , Muneta, T. , Arikawa-Hirasawa, E. , Sekiya, I. and Kaneko, K. Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium. , PLoS ONE 7 , e45517 , 2012
Mouse Mouse	Epithelial stem	Collagenase : 2%	DMEM/F12	Chavez, M. , Hu, J. , Seidel, K. , Li, C. , Jheon, A. , Naveau, A. , Horst, O. and Klein, O. Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor. , J Vis Exp , 2014
Mouse Mouse	Urothelial	Collagenase Type 1 : 0.5%	PBS	Huang, C. , Chen, C. and Shyr, C. The Anti-Tumor Effect of Intravesical Administration of Normal Urothelial Cells on Bladder Cancer. , Cytotherapy 19 , 1233-1245 , 2017
Mouse Mouse	Cochlear	Papain : 20 u/ml Trypsin : 0.05%	DMEM	Kim, Y. , Wang, S. , Tymanskyj, S. , Ma, L. , Tao, H. and Zhang, L. Dcc Mediates Functional Assembly of Peripheral Auditory Circuits. , Sci Rep 6 , 23799 , 2016
Mouse Mouse	Bone marrow stromal	Collagenase Type 1 : 0.2%	PBS	Ishida, T. , Suzuki, S. , Lai, C. , Yamazaki, S. , Kakuta, S. , Iwakura, Y. , Nojima, M. , Takeuchi, Y. , Higashihara, M. , Nakauchi, H. and Otsu, M. Pre-Transplantation Blockade of TNF-Î±-Mediated Oxygen Species Accumulation Protects Hematopoietic Stem Cells. , Stem Cells 35 , 989-1002 , 2017
Mouse Mouse, embryonic	Trachea	Elastase : 0.1% Neutral Protease : 0.25% Deoxyribonuclease I : 0.01% Trypsin : 0.125%	DMEM/F12	He, M. , Wu, B. , Ye, W. , Le, D. , Sinclair, A. , Padovano, V. , Chen, Y. , Li, K. , Sit, R. , Tan, M. , Caplan, M. , Neff, N. , Jan, Y. , Darmanis, S. and Jan, L. Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway. , Elife 9 , 53085 , 2020
Mouse Mouse	Adhesion fibroblasts	Collagenase Type 4 : 0.2%	Medium 199	Foster, D. , Marshall, C. , Gulati, G. , Chinta, M. , Nguyen, A. , Salhotra, A. , Jones, R. , Burcham, A. , Lerbs, T. , Cui, L. , King, M. , Titan, A. , Ransom, R. , Manjunath, A. , Hu, M. and Blackshear, C. Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation. , Nat Commun 11 , 4061 , 2020
Mouse Mouse, 25-30 g	Tracheal inflammatory cells	Collagenase Type 4 : 0.1% Deoxyribonuclease I : 50 u/ml Soybean Trypsin Inhibitor : 0.1%	RPMI 1640	Minamoto Kanji, Pinsky DavidJ. Recipient iNOS but not eNOS deficiency reduces luminal narrowing in tracheal allografts , J Exp Med 196 , 1321-33 , 2002
Mouse Mouse, 8-12 week	Urinary bladder	Collagenase Type 1 : 50 u/ml Collagenase Type 3 : 40 u/ml Papain : 45 u/ml Deoxyribonuclease I : 4 u/ml Collagenase Type 6 : 20 u/ml	Tyrode solution	Atta, H. , Gheinani, A. , Wacker, A. , Heshmati, Y. , Bigger-Allen, A. , Lambrinos, G. , Gao, Y. , Bielenberg, D. and Adam, R. A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury. , J Vis Exp , 2020
Mouse Mouse	Spleen, bone marrow endothelial	Collagenase Type 4 : 0.3-1.0% Deoxyribonuclease I : 20 u/ml	PBS	Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E. Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. , Immunity 34 , 590 , 2011
Mouse Mouse	Organoid cultures	Stemxyme 1 : 0.1%	PBS	Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S. Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Mouse Mouse, 6-8 week	Bone marrow mesenchymal stem	Collagenase Type 1 : 0.25%	RPMI 1640	Xu, S. , De Becker, A. , Van Camp, B. , Vanderkerken, K. and Van Riet, I. An Improved Harvest and In Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells. , J Biomed Biotechnol 2010 , 105940 , 2010
Mouse Mouse	Bone marrow	Collagenase Type 1 : 0.15% Neutral Protease : 0.15%	PBS	Bertoncello, I. and Williams, B. Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining. , Methods Mol Biol 263 , 181 , 2004
Mouse Mouse	Leucocytes	Collagenase Type 1 : 0.25% Deoxyribonuclease I : 0.002%	RPMI-1640	Hameed, M. , Rai, P. , Makris, M. and Weger-Lucarelli, J. Optimized Protocol for Mouse Footpad Immune Cell Isolation for Single-cell RNA Sequencing and Flow Cytometry. , STAR Protoc Vol. 4 , 102409 , 2023
Mouse Mouse	Dentritic	Collagenase Type 2 : 0.1% Deoxyribonuclease I : 0.001%	DME	Stock, A. , Booth, S. and Cerundolo, V. Prostaglandin E2 Suppresses the Differentiation of Retinoic Acid-Producing Dendritic Cells in Mice and Humans. , J Exp Med 208 , 761 , 2011
Mouse Mouse, 6-10 week	Salivary gland and stomach	Collagenase Type 4 : 0.8% Deoxyribonuclease I : 1.0%	RPMI-1640	Ji, H. , Rintelen, F. , Waltzinger, C. , Meier, D, Bilancio, A. , Pearce, W. , Hirsch, E. , Wymann, M. , Ruckle, T. , Camps, M. , Vanhaesebroeck, B. , Okkenhaug, K. and Rommel, C. Inactivation of PI3Kgamma and PI3Kdelta Distorts T-cell Development and Causes Multiple Organ Inflammation. , Blood 110 , 2940 , 2007
Porcine Porcine, 6 month	Synoviocytes	Hyaluronidase : 660 u/ml Trypsin : 0.25% Collagenase Type 2 : 583 u/ml	DMEM	Kean, T. and Dennis, J. Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions. , PLoS ONE 10 , e0129961 , 2015
Porcine Porcine, various ages	Nucleous pulposus and AF	Pronase : 0.05% Collagenase : 0.2%	Modified F-12K	Cho, H. , Park, S. , Lee, S. , Kang, M. , Hasty, K. and Kim, S. Snapshot of Degenerative Aging of Porcine Intervertebral Disc: A Model to Unravel the Molecular Mechanisms. , Exp Mol Med 43 , 334-40 , 2011
Porcine Porcine, <10 week	Nucleus pulposus	Collagenase : 0.05% Pronase : 0.3%	DMEM	Potier, E. and Ito. K. Can Notochordal Cells Promote Bone Marrow Stromal Cell Potential for Nucleus Pulposus Enrichment? A Simplified in Vitro System. , Tissue Eng Part A 20 , 3241-51 , 2014
Rabbit Rabbit, New Zealand, 8-10 week	Tenocytes and tendon stem cells	Collagenase Type 1 : 0.3% Neutral Protease : 0.4%	DMEM	Zhang, J. and Wang, J. Characterization of Differential Properties of Rabbit Tendon Stem Cells and Tenocytes. , BMC Musculoskelet Disord 11 , 10 , 2010
Rat Rat, 8-10 week	Synovial cells	Collagenase Type 1 : 250 u/ml	RPMI 1640	Moghaddami, M. , Cleland, L. and Mayrhofer, G. MHC II+ CD45+ Cells from Synovium-Rich Tissues of Normal Rats: Phenotype, Comparison with Macrophage and Dendritic Cell Lineages and Differentiation into Mature Dendritic Cells In Vitro. , Int Immunol 17 , 1103 , 2005
Rat Rat, Wistar, 7-9 week	Gingival mitochondria	Collagenase Type 1 : 0.115-0.130 %	HBSS	Kaneko, N. , Rikimaru, T. , Fujimura, T. , Mori, S. , Hidaka, S. and Kaya, H. Preparation of Rat Gingival Mitochondria with an Improved Isolation Method. , Int J Dent 2010 , 275103 , 2010
Rat Rat, male, 250-350g	Submandibular acinar	Collagenase : 0.05%	PBS	Silver, N. , Proctor, GB, Arno, M. and Carpenter GH Activation of mTOR Coincides with Autophagy During Ligation-induced Atrophy in the Rat Submandibular Gland. , Cell Death Dis 1 , e14 , 2010
Rat Rat, SD, 8-10 week	Oral fibroblasts	Collagenase Type 1 : 200 u/ml	DMEM	Lin, A. , Hokugo, A. , Choi, J. and Nishimura, I. Small Cytoskeleton-Associated Molecule, Fibroblast Growth Factor Receptor 1 Oncogene Partner 2/Wound Inducible Transcript-3.0 (FGFR1OP2/wit3.0), Facilitates Fibroblast-Driven Wound Closure. , Am J Pathol 176 , 108-21 , 2010
Rat Rat, Lewis, M	Synovial macrophages	Collagenase Type 1 : 250 u/ml	DMEM	Jain, S. , Tran, T. and Amiji, M. Macrophage Repolarization with Targeted Alginate Nanoparticles Containing IL-10 Plasmid DNA for the Treatment of Experimental Arthritis. , Biomaterials 61 , 162-77 , 2015
Rat Rat, SD, 6-8 month	Pluripotent tendon	Collagenase Type 1 : 0.3% Neutral Protease : 0.4%	DMEM	Zhou, B. , Zhou, Y. and Tang, K. The Effects of Substance P on Pluripotent Tendon Cells: an In Vitro and In Vivo Study. , J Musculoskelet Neuronal Interact 14 , 349-58 , 2014