Collagenase (Animal Free)

Source:
Clostridium histolyticum
CAS:
9001-12-1
EC:
3.4.24.3

• New! Animal Free Types AFA, AFB and AFC collagenases are derived from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of potential animal derived pathogens must be prevented. Levels of secondary proteases are similar to Types 1 and 2 collagenase.

• CLSAFA is the original AFA grade designed to have collagenase and secondary proteases similar to Types 1 and 2 collagenase.
• CLSAFB contains higher collagenase and caseinase activities than CLSAFA.
• CLSAFC has especially low tryptic activity similar to Type 4 collagenase.

 • CLSAFD contains two to three fold higher specific activity than CLSAFA

• Worthington also offers 0.22 micron filtered preparations of each type in 50 mg/vial pre-packaged form for direct reconstitution and use in all isolation procedures.

Collagenase (Animal Free) Products

Description
Activity
Code
Cat. #
Size
Price
Description
Collagenase CLSAFA AF
Source:
Clostridium histolyticum
Collagenase Animal Free/AF is prepared from cultures grown in a medium devoid of animal based components with similar collagenase and secondary proteases to CLS-1 and CLS-2.
Store at 2-8°C.
Activity
≥150 units per mg dry weight
Code
CLSAFA
LS004152
100 mg
$53.00
LS004154
1 gm
$258.00
LS004156
5 gm
$1,100.00
LS004158
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 50000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Collagenase CLSAFB AF
Source:
Clostridium histolyticum
Collagenase Animal Free/AF is prepared from cultures grown in medium devoid of animal based components to contain higher collagenase and caseinase activities than CLSAFA. 
Store at 2-8°C.
Activity
≥300 units per mg dry weight
Code
CLSAFB
LS004145
100 mg
$53.00
LS004147
1 gm
$258.00
LS004148
5 gm
$1,100.00
LS004150
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 50000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Collagenase CLSAFC AF
Source:
Clostridium histolyticum
Collagenase Animal Free/AF is prepared from cultures grown in a medium devoid of animal based components to contain especially low tryptic activity similar to CLS-4.
Store at 2-8°C.
Activity
≥200 units per mg dry weight
Code
CLSAFC
LS004138
100 mg
$53.00
LS004140
1 gm
$258.00
LS004141
5 gm
$1,100.00
LS004143
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 50000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Collagenase CLSAFD AF
Source:
Clostridium histolyticum
Collagenase Animal Free/AF is prepared from cultures grown in a medium devoid of animal based components to contain two to three fold higher specific activity than CLSAFA. 
Store at 2-8°C.
Code
CLSAFD
LS004160
100 mg
$68.00
LS004162
500 mg
$275.00
LS004163
2500 mg
$1,315.00
LS004165
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 25000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Collagenase, CLSAFA, Filtered, AF
Source:
Clostridium histolyticum
Collagenase, Animal Free/AF (Code: CLSAFA) which is filtered through a 0.22 micron membrane and lyophilized in vials.
Store at 2-8°C.
Activity
≥150 units per mg dry weight
Code
CLSAFAS
LS004118
50 mg
$72.00
LS004119
5x50 mg
$300.00
LS004123
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 10000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Collagenase, CLSAFB, Filtered, AF
Source:
Clostridium histolyticum
Collagenase, Animal Free/AF (Code: CLSAFB) which is filtered through a 0.22 micron membrane and lyophilized in vials.
Store at 2-8°C.
Activity
≥300 units per mg dry weight
Code
CLSAFBS
LS004124
50 mg
$72.00
LS004125
5x50 mg
$300.00
LS004129
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 10000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Collagenase, CLSAFC, Filtered, AF
Source:
Clostridium histolyticum
Collagenase Animal Free/AF (Code: CLSAFC) which is filtered through a 0.22 micron membrane and lyophilized in vials.
Store at 2-8°C.
Activity
≥200 units per mg dry weight
Code
CLSAFCS
LS004130
50 mg
$72.00
LS004131
5x50 mg
$300.00
LS004135
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 10000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Collagenase, Purified, Animal Free
Source:
Clostridium histolyticum
Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens must be prevented.  Chromatographically purified.  ≤ 50 caseinase units per milligram. Supplied as a lyophilized powder.
Store at 2-8°C.
Activity
≥1,500 units per mg dry weight
Code
CLSAFP
Minimum order quantity
100
LS005294
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 100 x 1 ku. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
LS005290
4 ku
$95.00
LS005292
10 ku
$195.00
Description
STEMxyme® 1, Collagenase/Neutral Protease (Dispase), AF
Source:
Clostridium histolyticum/Bacillus polymyxa
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and animal free Bacillus polymyxa neutral protease with a minimum of 250 CLS units and 1,000 caseinase units per mg dry weight. Designed for stem cell and other primary cell isolations and bioprocessing applications where introduction of potential animal derived pathogens must be prevented.
Store at 2-8°C.
Activity
≥250 CLS/1,000 CAS units per milligram dry weight
Code
STZ1
LS004106
50 mg
$110.00
LS004107
5x50 mg
$495.00
Description
STEMxyme® 2 Collagenase/Neutral Protease (Dispase), AF
Source:
Clostridium histolyticum/Bacillus polymyxa
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and animal free Bacillus polymyxa neutral protease with a minimum of 250 CLS units and 2,000 caseinase units per mg dry weight. Designed for stem cell and other primary cell isolations and bioprocessing applications where introduction of potential animal derived pathogens must be prevented.
Store at 2-8°C.
Activity
≥250 CLS/2,000 CAS units per milligram dry weight
Code
STZ2
LS004112
50 mg
$164.00
LS004113
5x50 mg
$725.00