Skin | Worthington Biochemical

Species	Cells	Enzyme(s)	Medium	Reference
Canine Canine	Epidermal	Collagenase : 0.35% Neutral Protease : 0.1%	DMEM	Zheng, Y. , Nace, A. , Chen, W. , Watkins, K. , Sergott, L. , Homan, Y. , Vandeberg, J. , Breen, M. and Stenn, K. Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis. , Dev Dyn 239 , 2619-26 , 2010
Frog Frog	Epidermal	Trypsin : 0.18%	Barth's solution, CMF	Nishikawa, A. , Shimizu-Nishikawa, K. and Miler, L. Isolation, characterization, and in vitro culture of larval and adult epidermal cells of the frog Xenopus laevis , In Vitro Cell Dev Biol 26 , 1128 , 1990
Goat Goat	Dermis isolated aggrecan sensitive	Neutral Protease : 0.5% Collagenase Type 2 : 200 u/ml	DMEM	Deng, Y. , Hu, J. and Athanasiou, K. Isolation and Chondroinduction of a Dermis-Isolated, Aggrecan-Sensitive Subpopulation with High Chondrogenic Potential. , Arthritis Rheum 56 , 168 , 2007
Human Human, fetal	Smooth muscle, fibroblasts	Trypsin : 0.055%	DMEM	Davies, P. and Kerr, C. Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts , Biochim Biophys Acta 712 , 26 , 1982
Human Human, neonatal	Keratinocytes	Neutral Protease : 0.4% Collagenase : 0.3%	DMEM	Li, A. , Pouliot, N. , Redvers, R. and Kaur, P. Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny , J Clin Invest 113 , 390-400 , 2004
Human Human, adult	Epidermal stem cells	Neutral Protease : 2 u/ml Trypsin : 2.5%	DMEM	Nagel, S. , Rohr, F. , Weber, C. , Kier, J. , Siemers, F. , Kruse, C. , Danner, S. , Brandenburger, M. and Matthiessen, A. Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro. , PLoS ONE 8 , e78365 , 2013
Human Human	Keratinocytes	Trypsin : 0.25%	(see reference)	Rheinwald, J. and Green, H. Serial Cultivation of Strains of Human Epidermal Kerotinocytes: The Formation of Keratinizing Colonies from Single Cells , Cell 6 , 331 , 1975
Human Human	Human skin mast cells	Neutral Protease : 0.1% Collagenase Type 1 : see reference	PBS	Grutzkau A, Kruger-Krasagakes S, Baumeister H, Schwarz C, Kogel H, Welker P, Lippert U, Henz BM, Moller A. Synthesis, storage, and release of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) by human mast cells: implications for the biological significance of VEGF206 , Mol Biol Cell 9 , 875-84 , 1998
Human Human	Skin	Neutral Protease : 5 u/ml Collagenase Type 3 : 0.3% Deoxyribonuclease I : 0.005%	RPMI 1640	Cheuk, S. , Wiken, M. , Blomqvist, L. , Nylen, S. , Talme, T. and Stahle, M.and Eidsmo, L. Epidermal Th22 and Tc17 Cells Form a Localized Disease Memory in Clinically Healed Psoriasis. , J Immunol 192 , 3111 , 2014
Human Human	Fibroblasts	Trypsin : 0.25%	CMF solution	Limat, A. , Hunziker, T. , Boillat, C. , Noser, F. and Wiesmann, U. Postmitotic Human Dermal Fibroblasts Preserve Intact Feeder Properties for Epithelial Cell Growth After Long-Term Cryopreservation , In Vitro Cell Dev Biol 26 , 709 , 1990
Human Human	Keratinocytes	Neutral Protease : 0.25% Trypsin : 0.17% Thermolysin : 0.05%	see reference	Hybbinette S, Bostrom M, Lindberg K. Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation , Exp Dermatol 8 , 30-8 , 1999
Human Human	Skin fibroblasts	Collagenase Type 1 : 1000 u/ml	DMEM	Douvaras, P. , Wang, J. , Zimmer, M. , Hanchuk, S. , O'Bara, M. , Sadiq, S. , Sim, F. , Goldman, J. and Fossati, V. Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells. , Stem Cell Reports 3 , 250 , 2014
Human Human, 18-50 years	Sweat duct	Collagenase : 0.03%	MEM	Bell, C. and Quinton, P. Effects of Media Buffer Systems on Growth and Electrophysiologic Characteristics of Cultured Sweat Duct Cells , In Vitro Cell Dev Biol 27 , 47 , 1991
Human Human	Epidermal	Collagenase Type 2 : 0.1% Neutral Protease : 0.5-1.0%	PBS	Reece J C, Handley A J, Anstee E J, Morrison W A, Crowe S M, Cameron P U. HIV-1 selection by epidermal dendritic cells during transmission across human skin , J Exp Med 187 , 1623-31 , 1998
Human Human	Fibroblasts	Neutral Protease : 0.1% Collagenase Type 1 : 0.25%	DMEM	Chen, Z. , Wang, X. , Jin, T. , Wang, Y. , Hong, C. , Tan, L. , Dai, T. , Wu, L. , Zhuang, Z. and Shi, C. Increase in the Radioresistance of Normal Skin Fibroblasts but not Tumor Cells by Mechanical Injury. , Cell Death Dis 8 , e2573 , 2017
Human Human	Melanocytes, skin/ foreskin	Trypsin : 0.25%	PBS	Peacocke, M. , Yaar, M. , Mansur, C. , Chao, M. and Gilchrest, B. Induction of Nerve Growth Factor Receptors on Cultured Human Melanocytes , Proc Natl Acad Sci U S A 85 , 5282 , 1988
Human Human	Mast cells	Neutral Protease : 0.05% Collagenase Type 4 : 1.0%	RPMI	Babina Magda, Guhl Sven, Starke Andrae, Kirchhof Loreen, Zuberbier Torsten, Henz Beate M. Comparative cytokine profile of human skin mast cells from two compartments--strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming , J Leukoc Biol 75 , 244-52 , 2004
Human Human	Fibroblasts and keratinocytes	Neutral Protease : 0.5% Collagenase Type 1 : 0.2%	DMEM	Carriel, V. , Garzon, I. , Jimenez, J. , Oliveira, A. , Arias-Santiago, S. , Campos, A. , Sanchez-Quevedo, M. and Alaminos, M. Epithelial and Stromal Developmental Patterns in a Novel Substitute of the Human Skin Generated with Fibrin-Agarose Biomaterials. , Cells Tissues Organs 196 , 1-12 , 2012
Human Human	Keratinocytes	Trypsin : 0.25%	DMEM	Dover, R. and Potten, C. Cell Cycle Kinetics of Cultured Human Epidermal Keratinocytes , J Invest Dermatol 80 , 423 , 1983
Human Human	Keratinocytes, fibroblasts, endothelial	Neutral Protease : see reference Collagenase : see reference	see reference	Supp Dorothy M, Wilson-Landy Kaila, Boyce Steven T. Human dermal microvascular endothelial cells form vascular analogs in cultured skin substitutes after grafting to athymic mice , FASEB J 16 , 797-804 , 2002
Human Human	Mononuclear phagocytes	Neutral Protease : 1 u/ml Deoxyribonuclease I : 50 u/ml Collagenase : 200 u/ml Trypsin : 0.5%	RPMI-1640	Botting, Ra. , Bertram, K. , Baharlou, H. , Sandgren, K. , Fletcher, J. , Rhodes, J. , Rana, H. , Plasto, T. , Wang, X. , Lim, J. , Barnouti, L. , Kohout, M. , Papadopoulos, T. , Merten, S. , Olbourne, N. and Cunningham, A. Phenotypic and Functional Consequences of Different Isolation Protocols on Skin Mononuclear Phagocytes. , J Leukoc Biol 101 , 1393-1403 , 2017
Human Human	Skin	Collagenase Type 4 : 0.08-0.2% Neutral Protease : 0.1% Deoxyribonuclease I : 0.005%	RPMI	Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K. Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. , PLoS Pathog 10 , e1004548 , 2014
Human Human	Human sweat glands	Collagenase : 0.2%	HBSS	Lee, C. , Carpenter, F. , Coaker, T. and Kealey, T. The Primary Culture of Epithelia From the Secretory Coil and Collecting Duct of Normal Human and Cystic Fibrotic Eccrine Sweat Glands , J Cell Sci 83 , 103 , 1986
Human Human	Fibroblasts	Trypsin : 0.25%	DMEM	Tuan Tai-Lan, Wu Huayang, Huang Eunice Y, Chong Sheree S N, Laug Walter, Messadi Diana, Kelly Paul, Le Anh. Increased plasminogen activator inhibitor-1 in keloid fibroblasts may account for their elevated collagen accumulation in fibrin gel cultures , Am J Pathol 162 , 1579-89 , 2003
Human Human	Diploid fibroblasts	Collagenase Type 1 : 0.03%	DMEM	Tan, J. , Jaafar, F. and Makpol, S. Proteomic Profiling of Senescent Human Diploid Fibroblasts Treated with gamma-tocotrienol. , BMC Complement Altern Med 18 , 314 , 2018
Human Human, ages 16-30	Human sweat duct	Collagenase Type 2 : 0.2%	RPMI 1640 (see reference)	Pedersen, P. Human Sweat Duct Cells in Primary Culture. Basic Bioelectric Properties of Cultures Derived From Normals and Patients with Cystic Fibrosis , In Vitro Cell Dev Biol 25 (4) , 342 , 1989
Human Human	Hair follicular epithelial	Neutral Protease : 1.25% Trypsin : 0.25%	DMEM/F-12	Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei. Isolation of a novel population of multipotent adult stem cells from human hair follicles , Am J Pathol 168 , 1879-88 , 2006
Human Human, adult, female, breast skin	Keratinocytes	Trypsin : 0.25%	PBS	Hirel, B. , Chesne, C. , Pailgheret, J. and Guillouzo, A. Expression of Differentiation Markers in Human Adult Keratinocytes Cultured in Submerged Conditions , In Vitro Cell Dev Biol 30A , 372 , 1994
Human Human, 6-12 year	Dermal fibroblasts	Neutral Protease : 0.1% Collagenase Type 1 : 0.1%	DMEM	Chen, F. , Zhang, W. , Bi, D. , Liu, W. , Wei, X. , Chen, F. , Zhu, L. , Cui, L. and Cao, Y. Clonal Analysis of Nestin(-) Vimentin(+) Multipotent Fibroblasts Isolated from Human Dermis. , J Cell Sci 120 , 2875 , 2007
Human Human, adult	Keratinocytes	Neutral Protease : 0.25%	PBS	Baudoux, B. , Castanares-Zapatero, D. , Leclercq-Smekens, M. , Berna, N. and Poumay, Y. The Tetraspanin CD9 Associates with the Integrin a6B4 in Cultured Human Epidermal Keratinocytes and is Involved in Cell Motility , Eur J Cell Biol 79 , 41 , 2000
Human Human, adult	Dermal fibroblasts	Collagenase : 0.5-1.0%	DMEM	Wang, H. , Van Blitterswijk, C. , Bertrand-De Haas, M. , Schuurman, A. and Lamme, E. Improved Enzymatic Isolation of Fibroblasts for the Creation of Autologous Skin Substitutes. , In Vitro Cell Dev Biol Anim 40 , 268 , 2004
Human Human, neonatal foreskin	Fibroblasts	Trypsin : 0.25%	DMEM	Hansbrough, J. , Cooper, M. , Cohen, R. , Spielvogel, R. , Greenleaf, G. , Bartel, R. and Naughton, G. Evaluation of a Biodegradable Matrix Containing Cultured Human Fibroblasts as a Dermal Replacement Beneath Meshed Skin Grafts on Athymic Mice , Surgery 111 (1) , 438 , 1992
Human Human	fibroblasts	Collagenase : 0.5% Trypsin : 0.25%	DMEM/F12	Clark, R. , Chong, B. , Mirchandani, N. , Yamanaka, K. , Murphy, G. , Dowgiert, R. and Kupper, T. A Novel Method for the Isolation of Skin Resident T cells from Normal and Diseased Human Skin. , J Invest Dermatol 126 , 1059 , 2006
Human Human, 18-30 years, male, female	Fibroblasts	Trypsin : 0.1%	HBSS	McCoy, B. , Galdun, J. and Cohen, I. Effects of Density and Cellular Aging On Collagen Synthesis and Growth Kinetics in Keloid and Normal Skin Fibroblasts , In Vitro 18 (1) , 79 , 1982
Human Human	Fibroblasts	Collagenase animal free : 2%	HBSS	Karumbayaram, S. , Lee, P. , Azghadi, S. , Cooper, A. , Patterson, M. , Kohn, D. , Pyle, A. , Clark, A. , Byrne, J. , Zack, J. , Plath, K. and Lowry, W. From Skin Biopsy to Neurons Through a Pluripotent Intermediate Under Good Manufacturing Practice Protocols , Stem Cells Transl Med 1 , 36 , 2012
Human Human, newborn	Keratinocytes	Trypsin : 0.3%	DMEM	Liu, S. , Eaton, M. and Karasek, M. Growth Characteristics of Human Epidermal Keratinocytes from Newborn Foreskin in Primary and Serial Cultures , In Vitro 15 (10) , 813 , 1979
Human Human	Epidermal	Collagenase : 0.35% Neutral Protease : 0.1%	DMEM	Zheng, Y. , Nace, A. , Chen, W. , Watkins, K. , Sergott, L. , Homan, Y. , Vandeberg, J. , Breen, M. and Stenn, K. Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis. , Dev Dyn 239 , 2619-26 , 2010
Human Human	Keratinocytes	Trypsin : 0.05%	Dulbecco's PBS	Judd, D. , Battista, P. and Behm, D. Culture of Human Keratinocytes in Defined Serum-Free Medium , Focus 19 (1) , 2 , 1997
Human Human, female, 18-66 yr	Fibroblasts	Trypsin : 0.2%	DMEM	Huschtscha, L. , Napier, C. , Noble, J. , Bower, K. , Au, A. , Campbell, H. , Braithwaite, A. and Reddel, R. Enhanced Isolation of Fibroblasts From Human Skin Explants. , Biotechniques 53 , 239 , 2012
Human Human, adult	Epidermal keratinocytes	Trypsin : 0.25% Collagenase : 0.2% Deoxyribonuclease I : 0.001%	Eagle's MEM	Alitalo K, Kuismanen E, Myllyla R, Kiistala U, Asko-Seljavaara S, Vaheri A. Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells , J Cell Biol 94 , 497-505 , 1982
Human Human	Keratinocytes	Trypsin : 0.25%	DMEM	Regnier, M. Culture of Human Karatinocytes , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons Ltd , 11B:4.1 , 1995
Human Human	Fibroblasts	Collagenase Type 1 : 100-320 u/ml	DMEM	Tuan, T. , Hwu, P. , Ho, W. , Yiu, P. , Chang, R. , Wysocki, A. and Benya, P. Adenoviral Overexpression and Small Interfering RNA Suppression Demonstrate that Plasminogen Activator Inhibitor-1 Produces Elevated Collagen Accumulation in Normal and Keloid Fibroblasts. , Am J Pathol 173 , 1311-25 , 2008
Human Human	Sweat gland	Collagenase Type 2 : 0.015%	(see reference)	Krouse, M. , Hagiqara, G. , Chen, J. , Lewiston, N. and Wine, J. Ion Channels in Normal Human and Cystic Fibrosis Sweat Gland Cells , Am J Physiol 257 , C129 , 1989
Human Human	Skin	Neutral Protease : 2.4 u/ml Collagenase Type 4 : 1,000 u/ml	RPMI	Burja, B. , Paul, D. , Tastanova, A. , Edalat, S. , Gerber, R. , Houtman, M. , Elhai, M. , Bürki, K. , Staeger, R. , Restivo, G. , Lang, R. , Sodin-Semrl, S. , Lakota, K. , Tomšič, M. , Levesque, M. , Distler, O. , Rotar, �. , Robinson, M. and Frank-Bertoncelj, M. An Optimized Tissue Dissociation Protocol for Single-Cell RNA Sequencing Analysis of Fresh and Cultured Human Skin Biopsies. , Front Cell Dev Biol Vol. 10 , 872688 , 2022
Human Human	Epidermis plus dermis Abdomen or inner forearm	Trypsin : 0.125%	DMEM, M199	Harley, C. and Sherwood, S. , Methods in Molecular Biology, Basic Cell Culture Protocols, 2nd ed. Vol. 75 , Pollard, J., and Walker, J. , Humana Press , 23 , 1997
Human Human	Skin and keloid progenitor	Collagenase Type 1 : 0.4% Neutral Protease : 0.3%	PBS	Zhang, Q. , Yamaza, T. , Kelly, AP, Shi, S. , Wang, S. , Brown, J. , Wang, L. , French, S. , Shi, S. and Le, A. Tumor-like Stem Cells Derived from Human Keloid are Governed by the Inflammatory Niche Driven by IL-17/IL-6 Axis. , PLoS ONE 4 , e7798 , 2009
Mouse Mouse	Dermal fibroblasts	Collagenase : 400 u/ml	AminoMAX II	Scheibye-Knudsen, M. , Ramamoorthy, M. , Sykora, P. , Maynard, S. , Lin, P. , Minor, R. , Wilson, D. , Cooper, M. , Spencer, R. , de Cabo, R. , Croteau, D. and Bohr, V. Cockayne Syndrome Group B Protein Prevents the Accumulation of Damaged Mitochondria by Promoting Mitochondrial Autophagy. , J Exp Med 209 , 855 , 2012
Mouse Mouse, 2-3 day	Dermal fibroblasts	Collagenase Type 2 : 0.04% Trypsin : 0.025%	DME	Farina, G. , York, M. , Di Marzio, M. , Collins, C. , Meller, S. , Homey, B. , Rifkin, I. , Marshak-Rothstein, A. , Radstake, T. and Lafyatis, R. Poly(I:C) Drives Type I IFN- and TGFÎ²-Mediated Inflammation and Dermal Fibrosis Simulating Altered Gene Expression in Systemic Sclerosis. , J Invest Dermatol 130 , 2583-93 , 2010
Mouse Mouse	Epidermal	Neutral Protease : 0.5% Collagenase Type 4 : 0.1%	DMEM	Gu, D. , Fan, Q. and Xie, J. Cell Population Analyses During Skin Carcinogenesis. , J Vis Exp , e50311 , 2013
Mouse Mouse	Epithelial stem	Trypsin : 0.25% Collagenase Type 1 : 0.4%	HBSS	Jensen, U. , Ghazizadeh, S. and Owens, D. Isolation and Characterization of Cutaneous Epithelial Stem Cells. , Methods Mol Biol 989 , 61-9 , 2013
Mouse Mouse	Dermal fibroblasts	Elastase : 0.012% Collagenase Type 4 : 0.4%	DMEM	Rinkevich, Y. , Walmsley, G. , Hu, M. , Maan, Z. , Newman, A. , Drukker, M. , Januszyk, M. , Krampitz, G. , Gurtner, G. , Lorenz, H. , Weissman, I. and Longaker, M. Identification and Isolation of a Dermal Lineage with Intrinsic Fibrogenic Potential. , Science 348 , aaa2151 , 2015
Mouse Mouse	Mast	Collagenase Type 4 : 0.2%	IMDM	Antsiferova, M. , Martin, C. , Huber, M. , Feyerabend, T. , Forster, A. , Hartmann, K. , Rodewald, H. , Hohl, D. and Werner, S. Mast Cells are Dispensable for Normal and Activin-Promoted Wound Healing and Skin Carcinogenesis. , J Immunol 191 , 6147-55 , 2013
Mouse Mouse	Keratinocytes	Trypsin : 0.25% Collagenase Type 1 : 0.12% Collagenase Type 2 : 0.05% Collagenase Type 4 : 0.05% Hyaluronidase : 0.1%	Low calcium containing media	Blanco, S. , Bandiera, R. , Popis, M. , Hussain, S. , Lombard, P. , Aleksic, J. , Sajini, A. , Tanna, H. , Cortes-Garrido, R. , Gkatza, N. , Dietmann, S. and Frye, M. Stem Cell Function and Stress Response are Controlled by Protein Synthesis. , Nature 534 , 335-40 , 2016
Mouse Mouse	Keratinocytes	Collagenase Type 4 : 0.2% Deoxyribonuclease I : 0.005% Neutral Protease : 5 u/ml	DMEM	Spidale, N. , Malhotra, N. , Frascoli, M. , Sylvia, K. , Miu, B. , Freeman, C. , Stadinski, B. , Huseby, E. and Kang, J. Neonatal-Derived IL-17 Producing Dermal gd T Cells are Required to Prevent Spontaneous Atopic Dermatitis , Elife 9 , 5118 , 2020
Mouse Mouse, male 6-24 week	Skin side population	Collagenase Type 4 : 0.2% Neutral Protease : 1.2 u/ml	PBS	Montanaro F, Liadaki K, Volinski J, Flint A. and Kunkel LM. Skeletal muscle engraftment potential of adult mouse skin side population cells , Proc Natl Acad Sci U S A 100 , 9336 , 2003
Mouse Mouse	Skin	Collagenase Type 4 : 0.08-0.2% Neutral Protease : 0.1% Deoxyribonuclease I : 0.005%	RPMI	Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K. Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. , PLoS Pathog 10 , e1004548 , 2014
Mouse Mouse, 3-7 month	Fibroblasts, mesangial, smooth muscle	Trypsin : 0.25% Collagenase : see reference Soybean Trypsin Inhibitor : 0.05%	DMEM	Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH. Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation , Mol Biol Cell 10 , 1569-79 , 1999
Mouse Mouse	Ear epidermal	Trypsin : 0.1% Collagenase : 0.2%	PBS	Takanami-Ohnishi Yoko, Amano Shinya, Kimura Sadao, Asada Sachie, Utani Atsushi, Maruyama Masumi, Osada Hiroyuki, Tsunoda Hajime, Irukayama-Tomobe Yoko, Goto Katsutoshi, Karin Michael, Sudo Tatsuhiko, Kasuya Yoshitoshi. Essential role of p38 mitogen-activated protein kinase in contact hypersensitivity , J Biol Chem 277 , 37896-903 , 2002
Mouse Mouse, neonatal	Dermal	Collagenase : 0.35% Deoxyribonuclease I : see reference	PBS	Crigler Lauren, Kazhanie Amita, Yoon Tae-Jin, Zakhari Julia, Anders Joanna, Taylor Barbara, Virador VictoriaM. Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages , FASEB J 21 , 2050-63 , 2007
Mouse mouse	Dermal fibroblasts	Trypsin : 0.25% Collagenase Type 1 : 0.25%	DMEM	Baxter Ruth M, Crowell Thomas P, McCrann Margaret E, Frew Erica M, Gardner Humphrey. Analysis of the tight skin (Tsk1/+) mouse as a model for testing antifibrotic agents , Lab Invest 85 , 1199-209 , 2005
Mouse Mouse	Dermal	Collagenase Type 3 : 0.3% Deoxyribonuclease I : 0.0005%	HBSS	Eidsmo, L. , Allan, R. , Caminschi, I. , Van Rooijen, N. , Heath, W. and Carbone, F. Differential Migration of Epidermal and Dermal Dendritic Cells During Skin Infection. , J Immunol 182 , 3165 , 2009
Mouse Mouse, neonatal	Microvascular endothelial	Neutral Protease : 0.005% Collagenase Type 1 : 4%	DMEM	Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M. A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. , Microvasc Res 70 , 198 , 2005
Mouse Mouse	Epidermal and dermal	Collagenase Type 4 : 0.18% Collagenase/Dispase : 0.18%	PBS	King, I. , Kroenke, M. and Segal, B. GM-CSF-Dependent, CD103+ Dermal Dendritic Cells Play a Critical Role in Th Effector Cell Differentiation After Subcutaneous Immunization. , J Exp Med 207 , 953 , 2010
Porcine Porcine, 2-6 month	Keratinocytes	Neutral Protease : 0.25%	Dulbecco-Vogt MEM	Regauer S, Compton C. Cultured porcine epithelial grafts: an improved method , J Invest Dermatol 94 , 230-4 , 1990
Porcine Porcine, 3-4 month	Synovial membrane and skin stem	Collagenase : 0.2%	DMEM	Ando, W. , Kutcher, J. , Krawetz, R. , Sen, A. , Nakamura, N. , Frank, C. and Hart, D. Clonal Analysis of Synovial Fluid Stem Cells to Characterize and Identify Stable Mesenchymal Stromal Cell/Mesenchymal Progenitor Cell Phenotypes in a Porcine Model: A Cell Source with Enhanced Commitment to the Chondrogenic Lineage. , Cytotherapy 16 , 776 , 2014
Rat Rat, SD, male, 40-60 days	Sebaceous	Trypsin : 0.2%	DMEM with FBS PBS	Laurent, S. , Mednieks, M. and Rosenfield, R. Growth of Sebaceous Cells in Monolayer Culture , In Vitro Cell Dev Biol 28 , 83 , 1992
Rat Rat, neonatal, 12-24 hr post partum	Fibroblasts	Trypsin : 0.2%	HEPES buffered DMEM	Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R. Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF , Neuron 7 , 265 , 1991
Rat Rat, albino, one day old, CFN	Keratinocytes	Trypsin : 1%	EBSS	Vaughan, F. , Gray, R. and Bernstein, I. Growth and Differentiation of Primary Rat Keratinocytes on Synthetic Membranes , In Vitro Cell Dev Biol 22 (3) , 141 , 1986
Rat Rat, Wistar, 4-8 day	Dermal fibroblasts and keratinocytes	Trypsin : 0.25%	Ham's F-12	Sugihara, H. and Toda, S Primary Tissue Intact and Dissociated Cell Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 3A:2.1 , 1995