Mammary

Species	Cells	Enzyme(s)	Medium	Reference
Bovine Bovine, dairy, purebred, (also rat, Holtzmann, albino, white)	Secretory Mammary gland	Collagenase : 0.02 - 0.03%	HBSS or EBSS	Schingoethe, D. , Hageman, E. and Larson, B. Essential Amino Acids for Milk Protein Synthesis in the In Vitro Secretory Cell and Stimulation by Elevated Levels , Biochim Biophys Acta 148 , 469 , 1967
Bovine Bovine, young, lactating, female	Mammary	Collagenase : 0.30 %	HBSS or EBSS	Anderson, C. and Larson, B. Comparative Maintenance of Function in Dispersed Cell and Organ Cultures , Exp Cell Res 61 , 24 , 1970
Bovine Bovine	Epithelial	Hyaluronidase : 0.005%	HBSS	Gibson, C. , Vega, J. , Baumrucker, C. , Oakley, C. and Welsch, C. Establishment And Characterization Of Bovine Mammary Epithelial Cell Lines , In Vitro Cell Dev Biol 27 , 585 , 1991
Bovine Bovine	Epithelial	Deoxyribonuclease I : 0.04%	HBSS/Medium 199	Baumrucker, C. , Deemer, K. , Walsh, R. , Riss, T. and Akers, R. Primary Culture of Bovine Mammary Acini on a Collagen Matrix , Tissue Cell 20 (4) , 541 , 1988
Bovine Bovine, heifer, 200kg	Mammary epithelial	Collagenase Type 2 : 1% Hyaluronidase : 1% Deoxyribonuclease I : 0.03%	M-199	Weber M. , Purup S. , Vestergaard M. , Ellis S. , Scndergard-Andersen J. , Akers R. and Sejrsen K. Contribution of Insulin-like Growth Factor (IGF)-I and IGF-binding Protein-3 to Mitogenic Activity in Bovine Mammary Extracts and Serum , J Endocrinol 161 , 365 , 1999
Bovine Bovine, lactating Holstein	Mammary epithelial	Collagenase Type 3 : 400 u/ml Hyaluronidase : 100 u/ml Deoxyribonuclease I : 2 u/ml	HBSS	Miranda, S. , Wang, Y. , Purdie, N. , Osborne, V. , Coomber, B. and Cant, J. Selenomethionine Stimulates Expression of Glutathione Peroxidase 1 and 3 and Growth of Bovine Mammary Epithelial Cells in Primary Culture. , J Dairy Sci 92 , 2670 , 2009
Goat Goat, lactating, 1 month	Mammary gland	Collagenase : 0.02 - 0.03%	HBSS or EBSS	Blanco, A. , Rife, U. and Larson, B. Lactate Dehydrogenase Isozymes during Dedifferentiation in Cultures of Mammary Secretory Cells , Nature 214 , 1331 , 1967
Guinea-Pig Guinea-pig, pregnant, 4-10 day	Mammary gland	Trypsin NF 1:250 : 0.25%	Dulbecco phosphate	Turba, F. and Hilpert, N. Secretion and Resorption of Proteins by Isolated Mammary Gland Cells. German , Biochem Z 334 , 501 , 1961
Human Human, female, 15-61 yrs old	Epithelial	Hyaluronidase : 150 IU/ml	DMEM	Berthon, P. , Pancino, G. , Cremoux, P. , Roseto, A. , Gespach, C. and Calvo, F. Characterization of Normal Breast Epithelial Cells in Primary Cultures: Differentiation and Growth Factor Receptors Studies , J Tiss Cul Meth 28A , 716 , 1992
Human Human, normal biopsy	Fibroblasts	Collagenase Type 3 : 900 u/ml	DMEM/F-12	Ronnov-Jessen L. , Villadsen R. , Edwards J. and Petersen O. Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-beta1-mediated Conversion of Fibroblasts to Myofibroblasts , Am J Pathol 161 , 471 , 2002
Human Human	Mammary epithelial cells	Collagenase Type 1 : 0.1%	DMEM	Huss, F. and Kratz, G. Mammary Epithelial Cell and Adipocyte Co-Culture in a 3-D Matrix: The First Step Towards Tissue-Engineered Human Breast Tissue. , Cells Tissues Organs 169 , 361-7 , 2001
Human Human	Mammary epithelial	Collagenase : 200 u/ml Hyaluronidase : 100 u/ml	DMEM/F-12	Labarge, M. , Garbe, J. and Stampfer, M. Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture. , J Vis Exp 71 , 2013
Human Human	Epithelial	Collagenase : 2.0%	DMEM/Ham's F-12	Emerman, J. and Wilkinson, D. Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples , In Vitro Cell Dev Biol 26 , 1186 , 1990
Human Human	Myofibroblasts	Collagenase : 900 IU/ml	DME - F12	Ronnov-Jessen, L. , VanDeurs, B. , Nielsen, M. and Petersen, O.W. Identification, Paracrine Generation, and Possible Function of Human Breast Carcinoma Myofibroblasts in Culture , In Vitro Cell Dev Biol 28 , 273 , 1992
Human Human	Tumor, breast	Neuraminidase : 0.8 u/ml	HBSS	Leung, C. and Shiu, R. Morphological and Proliferative Characteristics of Human Breast Tumor Cells Cultured on Plastic and in Collagen Matrix , In Vitro 18 , 476 , 1981
Human Human	Epithelial	Hyaluronidase : 100 u/ml	DMEM/Ham's F-12	Stampfer, M. , Hallowes, R. and Hackett, A. Growth of Normal Human Mammary Cells in Culture , In Vitro 16 (5) , 415 , 1980
Human Human	Epithelial Fibroblasts	Collagenase Type 1 : 450 IU/ml	DMEM/Ham's F-12	Ogmundsdottir, H. , Petursdottir, I. , Gudmundsdottir, I. , Amundadottir, L. , Ronnov-Jessen, L. and Petersen, O. Effects of Lymphocytes and Fibroblasts on the Growth of Human Mammary Carcinoma Cells Studied in Short-Term Primary Cultures , In Vitro Cell Dev Biol 29A , 936 , 1993
Mouse Mouse, pregnant, 14-17 day	Mammary	Collagenase : 0.05% - 0.1%	HBSS	Daniel, C. and DeOme, K. Growth of Mouse Mammary Glands In Vivo After Monolayer Culture , Science 149 , 634 , 1965
Mouse Mouse, lactating	Parenchymal	Collagenase Type 1 : 0.3%	Kreb's Ringer bicarbonate buffer	Kerkof, P. and Abraham, S. Preparation of Adipose Cell-Free Suspensions of Mammary Gland Parenchymal Cells from Lactating Mice , Methods Enzymol 69 , 693 , 1976
Mouse Mouse, female	Mammary epithelial	Collagenase Type 3 : 300 u/ml Hyaluronidase : 100 u/ml Neutral Protease : 0.5% Deoxyribonuclease I : 0.001%	DMEM/F-12	Sun, H. , Xu, X. and Deng, C. Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands. , Bio Protoc 10 , e3530 , 2020
Mouse Mouse, BALB/cfC3H/Crgl, virgin, female	Nodule-transformed	Hyaluronidase : 0.1%	Medium 199	DeOme, K. , Miyamoto, M. , Osborn, R. , Guzman, R. and Lum, K. Detection of Inaparent Nodule-transformed Cells in the Mammary Gland Tissues of Virgn Female BALB/cfC3H Mice , Cancer Res 38 , 2103 , 1978
Mouse Mouse, female	Epithelial	Deoxyribonuclease I : 0.1%	DMEM	Jones, W. and Hallowes, R. Isolation of the Epithelial Subcomponents of the Mouse Mammary Gland for Tissue-Level Culture Studies , J Tiss Cul Meth 8 (1) , 17 , 1983
Mouse Mouse, lactating, 14 day	Mammary	Trypsin NF 1:250 : 0.25%	HBSS	Kopelovich, L. , Abraham, S. , McGrath, H. , DeOme, K. and Chaikoff, I. Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland , Cancer Res 26 , 1534 , 1966
Mouse Mouse, BALB/cCr1, female	Epithelial	Collagenase Type 2 : 0.2%	HBSS/DMEM	Asch, B. , Burstein, N. , Vidrich, A. and Sun, T. Identification of Mouse Mammary Epithelial Cells by Immunofluorescence With Rabbit and Guinea-Pig Antikeratin Antisera , Proc Natl Acad Sci U S A 78 , 5643 , 1981
Mouse Mouse (C3H/HeN), female	Epithelial	Deoxyribonuclease I : 0.0001%	Medium 199	Taketani, Y. and Oka, T. EGF Stimulates Cell Proliferation and Inhibits Functional Differentiation of Mouse Mammalian Dipithelial Cells in Culture , Endocrinology 113 , 871 , 1983
Mouse Mouse, BALB/c, female, 6-8 week	Epithelial	Collagenase Type 3 : 0.1%	DMEM	Ehmann, U. , Peterson, W. and Misfeldt, D. To Grow Mouse Mammary Epithelial Cells in Culture , J Cell Biol 98 , 1026 , 1984
Mouse Mouse, adult, 1-10 days pregnant	Epithelial Mammary	Collagenase : 0.02%	Simm's	Lasfargues, E. Cultivation and Behavior In Vitro of the Normal Mammary Epithelium of the Adult Mouse. II. Observations on the Secretory Activity , Exp Cell Res 13 , 553 , 1957
Mouse Mouse, (C3H/Crgl or BALB/cCrgl), 8-10 day pregnant	Epithelial	Collagenase Type 3 : 0.12%	HBSS	Emerman, J. , Enami, J. , Pitelka, D. and Nandi, S. Hormonal Effects on Intracellular and Secreted Casein in Cultures of Mouse Mammary Epithelial Cells on Floating Collagen Membranes , Proc Natl Acad Sci U S A 74 (10) , 4466 , 1977
Mouse Mouse, lactating, 14-18 day	Parenchymal	Collagenase : 0.33%	Kreb's buffer	Pitelka, D. , Kerkof, P. , Gagne, H. , Smith, S. and Abraham, S. Characteristics of Cells Dissociated from Mouse Mammary Glands. I. Method of Separation and Morphology of Parenchymal Cells from Lactating Glands , Exp Cell Res 57 , 43 , 1969
Mouse Mouse, BALB/C	Epithelium	Collagenase : 250 u/ml	HBSS	Kanazawa, T. and Hosick, H. Transformed Growth Phenotype of Mouse Mammary Epithelium in Primary Culture Induced by Specific Fetal Mesenchymes , J Cell Physiol 153 , 381 , 1992
Mouse Mouse, CBA, virgin, 9/10 wk	Mammary	Hyaluronidase : 0.1%	BSS, CMF	Prop, F. and Wiepjes, G. Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse , Exp Cell Res 61 , 451 , 1970
Mouse Mouse, BALB/c, virgin, female, 4 month	Adipocytes	Trypsin : 50 µg/ml	DMEM	Beck, J. , Hosick, H. and Watkins, B. Growth of Epithelium From a Preneoplastic Mammary Outgrowth on Response to Mammary Adipose Tissue , In Vitro Cell Dev Biol 25 (5) , 409 , 1989
Mouse Mouse (BALB/cCrgl), virgin, female, 4-8 month	Epithelial Mammary gland	Collagenase Type 3 : 0.1%	HBSS	White, M. , Hu, A. , Hamamoto, S. and Nandi In vitro analysis of proliferating epithelial cell populations from the mouse mammary gland: fibroblast-free growth and serial passage , In Vitro 14 , 271 , 1978
Mouse Mouse, BALB/cCrgl, female (also guinea-pig)	Swiss 3T3	Collagenase Type 3 : 0.2%	PBS CMF	Asch, B. and Medina, D. Concanavalin A-Induced Agglutinability of Normal, Preneoplastic, and Neoplastic Mouse Mammary Cells , J Natl Cancer Inst 61 (6) , 1423 , 1978
Mouse Mouse (BALB/c), pregnant, 60-80 days	Mammary	Hyaluronidase : 0.1%	CMF medium	Ceriani, R. , Peterson, J. and Abraham, S. Removal of Cell Surface Material by Enzymes Used to Dissociate Mammary Gland Cells , In Vitro 14 , 887 , 1978
Mouse Mouse, pregnant 8-12 day	Mammary	(see reference)	HBSS	Yang J, Richards J, Guzman R, Imagawa W. and Nandi S. Sustained growth in primary culture of normal mammary epithelial cells embedded in collagen gels , Proc Natl Acad Sci U S A 77 , 2088 , 1980
Mouse Mouse (BALB/cfC3H)	Mammary tumors Epithelial	Collagenase : 1.0%	HBSS	Yang, J. , Guzman, R. , Richards, J. and Nandi, S. Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels , In Vitro 16 , 502 , 1980
Mouse Mouse	Mammary epithelial stem	Collagenase Type 3 : 0.1% Hyaluronidase : 0.1% Pronase : 1.25% Deoxyribonuclease I : 0.2%	DMEM	Boulanger CA, Smith GH. Reducing mammary cancer risk through premature stem cell senescence , Oncogene 20 , 2264-72 , 2001
Mouse Mouse (NMuMG), female, 2 months	Epithelial	Collagenase Type 3 : 0.1%	DMEM	Ehmann, U. and Misfeldt, D. Mouse Mammary Cells in D-Valine Medium , In Vitro 18 , 407 , 1982
Mouse Mouse, 12 week	Mammary epithelial	Collagenase : 0.3% Hyaluronidase : 100 u/ml Trypsin : 0.25% Neutral Protease : 0.5% Deoxyribonuclease I : 0.01%	see reference	Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M. Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells , Nat Cell Biol 10 , 716-22 , 2008
Mouse Mouse, (BALB/c/Crgl Me), female, pregnant	Epithelial	Pepsin : 0.1% and 0.05% Pepsin : 0.1%	HBSS with 0.2% EDTA, CMF	Riser, M. , Huff, B. and Medina, D. Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells , In Vitro 19 , 730 , 1983
Mouse Mouse, 11 week	Epithelial	Collagenase Type 3 : 25 u/ml Hyaluronidase : 0.1% Protease XIV : 0.05% Deoxyribonuclease I : 0.04%	DMEM/F12	Mueller, S. , Clark, J. , Myers, P. and Korach, K. Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. , Endocrinology 143 , 2357 , 2002
Mouse Mouse, female, early pregnancy <10 days	Epithelial	Collagenase : 0.1%	Eagle's MEM	Moore, D. and Lasfargues, E. Method for the Continuous Cultivation of Mammary Epithelium , In Vitro 7 , 21 , 1971
Mouse Mouse, 10 week	Fibroblasts, carcinoma	Collagenase Type 4 : 0.5% Trypsin : 0.2% Deoxyribonuclease I : 0.004 Hyaluronidase : 1,000 u/ml	DMEM	Cheng, N. and Lambert, D. Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice. , J Vis Exp 54 , e2716 , 2011
Mouse Mouse (BALB/cCrgl), mature	Epithelial	Pronase : 0.01%	Hepes buffered medium 199	Bandyopadhyay, G. , Imagawa, W. , Wallace, D. and Nandi, S. Proliferative effects of insulin and epidermal growth factor on mouse mammary epithelial cells in primary culture , J Biol Chem 263 , 7567 , 1988
Mouse Mouse	Mammary tumor	Collagenase : 0.15% Hyaluronidase : 0.020%	DMEM/F12	Liu, X. , Johnson, S. , Liu, S. , Kanojia, D. , Yue, W. , Singh, U. , Wang, Q, Wang Qi, Nie, Q. and Chen H. Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy. , Sci Rep 3 , 2473 , 2013
Rat Rat, SD, 15-20 day postpartum	Mammary	Collagenase : 0.2%	Kreb's Ringer bicarbonate buffer	Martin, R. and Baldwin, R. Effects of Insulin on Isolated Rat Mammary Cell Metabolism: Glucose Utilization and Metabolite Patterns , Endocrinology 89 , 1263 , 1971
Rat Rat	Mammary epithelial	Collagenase Type 3 : 0.35%	HBSS	Mei, N. , McDaniel, L. , Dobrovolsky, V. , Guo, X. , Shaddock, J. , Mittelstaedt, R. , Azuma, M. , Shelton, S. , McGarrity, L. , Doerge, D. and Heflich, R. The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats. , Toxicol Sci 115 , 412 , 2010
Rat Rat, 50-60 days	Epithelial	Neutral Protease : 0.2%	EBSS	Hahm, H.A. and Ip, M.M. Primary Culture of Normal Rat Mammary Epithelial Cells Within a Basement Matrix. 1. Regulation of Proliferation by Hormones and Growth Factors , In Vitro Cell Dev Biol 26 , 791 , 1990
Rat Rat, Lewis, female, 90 days	Epithelial Mammary	Collagenase : 0.05%	Medium 199	Lin, T. , Hom, Y.K. , Richards, J. and Nandi, S. Effects of Antioxidants and Reduced Oxygen Tension on Rat Mammary Epithelial Cells in Culture , In Vitro Cell Dev Biol 27A , 191 , 1991
Rat Rat, SD, female, 55 day	Epithelial	Neutral Protease : 3 u/ml	Medium 199	Ehmann, U. , Osborn, R. , Guzman, R. and Fajardo, L. Cultured Proliferating Rat Mammary Epithelial Cells , In Vitro Cell Dev Biol 27 , 749 , 1991
Rat Rat (also human)	Epithelial	Collagenase Type 1 : 0.4%	DMEM	Soriano, J. , Pepper, M. , Nakamura, T. , Orci, L. and Montesano, R. Hepatocyte Growth Factor Stimulates Extensive Development of Branching Duct-like Structures by Cloned Mammary Gland Epithelial Cells , J Cell Sci 108 , 413-430 , 1995
Rat Rat, SD, female, 60-90 day	Epithelial	Collagenase Type 3 : 0.5%	EBSS	Laduca, J. and Sinha, D. In Vitro Carcinogenesis of Mammary Epithelial Cells by N-Nitroso-N-Methylurea Using a Collagen Gel Matrix Culture , In Vitro Cell Dev Biol 29A , 789 , 1993
Rat Rat, Fisher 344, virgin, 80-100 day	Epithelial	Hyaluronidase : 0.1%	Medium 199	McGrath, M. , Palmer, S. and Nandi, S. Differential Response of Normal Rat Mammary Epithelial Cells to Mammogenic Hormones and EGF , J Cell Physiol 125 , 182 , 1985
Rat Rat, LEW, virgin, female, 45-50 day	Epithelial	Collagenase : 0.1%	Medium 199	Ethler, S. Primary Culture and Serial Passage of Normal and Carcinogen-Treated Rat Mammary Epithelial Cells In Vitro , J Natl Cancer Inst 74 (6) , 1307 , 1985
Rat Rat, inbred LEW, female, 50-60 day old	Epithelial	Collagenase Type 3 : 0.1%	Medium 199	Richards, J. and Nandi, S. Primary Culture of Rat Mammary Epithelial Cells. I. Effect of Plating Density, Horomones, and Serum on DNA Synthesis , J Natl Cancer Inst 61 (3) , 765 , 1978
Rat Rat, virgin	Mammary	Collagenase : 0.35%	Medium 199	Moon, R. , Janns, D. and Young, S. Preparation of Fat Cell-"Free" Rat Mammary Gland , J Histochem Cytochem 17 (3) , 182 , 1969
Rat Rat, Wistar, 13-18 day postpartum	Acini	Collagenase : 0.05%	HBSS	Katz, J. , Wals, P. and Van de Velde, R Lipogenesis by Acini from Mammary Gland of Lactating Rats , J Biol Chem 249 , 7348 , 1974
Rat Rat, female	Mammary gland epithelial	Collagenase Type 3 : 0.35%	see reference	Djuric Z. , Lewis S. , Mayhugh M. , Naegeli L. , Tang N. and Hart R. Effect of Varying Caloric Restriction Levels on Female Rat Growth and 5-hydroxymethyl-2'-deoxyuridine in DNA , Toxicol Sci 66 , 125 , 2002
Rat Rat, SD	Mammary fibroblasts	Collagenase Type 3 : 0.2% Neutral Protease : 0.2% Deoxyribonuclease I : 0.01%	DMEM/F-12	Brake P. , Zhang L. and Jefcoate C. Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids , Mol Pharmacol 54 , 825 , 1998
Rat Rat, female, 50 day	Mammary epithelial	Collagenase Type 3 : 0.15%	DMEM/F12	Maffini M. , Soto A. , Calabro J. , Ucci A. and Sonnenschein C. The Stroma as a Crucial Target in Rat Mammary Gland Carcinogenesis , J Cell Sci 117 , 1495 , 2004
Rat Rat, SD, 50 day	Mammary epithelial	Collagenase Type 3 : 0.2% Neutral Protease : 0.2%	DMEM/F12	Varela L. , Darcy K. and Ip M. The Epidermal Growth Factor Receptor is not Required for Tumor Necrosis Factor-alpha Action in Normal Mammary Epithelial Cells , Endocrinology 138 , 3891 , 1997
Rat Rat, SD, female	Epithelial, cancer and tumor	Collagenase : 0.1%	Eagles's MEM	Cohen, L. Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma , In Vitro 18 , 565 , 1982