Epithelial

Species	Cells	Enzyme(s)	Medium	Reference
Bovine Bovine, fetal	Epithelial Tracheal	Neutral Protease : 2%	Dissociation medium, CMF	Schumann, B.L. , Cody, T.E. , Miller, M.L. and Leikauf, G.D. Isolation, Characterization, and Long-Term Culture of Fetal Bovine Tracheal Epithelial Cells , In Vitro Cell Dev Biol 24 , 211 , 1988
Canine Dog	Tracheal	Pronase : 0.1%	DMEM	Virmani, A. , Naziruddin, B. , Desai, V. , Lowry, J. , Graves, D. and Sachdev, G. Evidence for Secretion of High Molecular Weight Mucins by Canine Tracheal Epithelial Cells in Primary Culture: Effects of Select Secretagogues on Mucin Secretion , In Vitro Cell Dev Biol 28A , 120 , 1992
Chicken Chick, 5 day old	Intestinal mesenchymal and epithelial	Collagenase : 0.03%	DMEM	Simon-Assmann, P. and Kedinger, M Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Fish Shark (Squalus acanthias)	Rectal gland	Collagenase : 0.2%	Ringer's solution	Karnaky, Jr. , Valentich, J.D. , Currie, M.G. , Oehlenschlager, W.F. and Kennedy, M.P. Atriopeptin Stimulates Chloride Secretion in Cultured Shark Rectal Gland Cells , Am J Physiol 260 , 1125 , 1991
Fish Winter flounder, 200-500 g (Pseudopleuronectes americanus)	Renal tubule	Trypsin : 0.2% Trypsin : 0.2%	CMF solution	Dickman, K. and Renfro, J. Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport , Am J Physiol 251 , 424 , 1986
Fish Shark (Squalus acanthias)	Rectal gland	Collagenase : 0.2%	Ringer's solution	Valentich, J. Primary Cultures of Shark Rectal Gland Epithelial Cells: A Model for Hormone-Sensitive Chloride Transport , J Tiss Cul Meth 13 , 149 , 1991
Frog Frog, Xenopus laevis, adult, female	Colonic epithelial	Collagenase Type 4 : 0.1%	Kreb's	Heinke, B. and Clauss, W. Potassium Conductances in Isolated Single Cells from Xenopus Laevis Colonic Epithelium , J Comp Physiol [B] 169 , 148 , 1999
Guinea-Pig Guinea-pig, Hartley albino, 500-600 g	Endometrial	Collagenase : 0.25%	HBSS	Chaminadas, G. , Alkhalaf, M. , Remy-Martin, J.P. , Propper, A.Y. and Adessi, G.L. Specific Effect of Oestrone Sulphate on Protein Synthesis and Secretion by Cultured Epithelial Cells from Guinea-pig Endometrium , J Endocrinol 123 , 233 , 1989
Guinea-Pig Guinea-pig, Hartley, female, 200 g	Epithelial	Collagenase Type 1 : 0.1%	DMEM	Rutten, M. Use of Commerically Available Cell Culture Inserts for Primary Culture and Electrophysiologic Studies of Guinea Pig Gastric Mucous Epithelial Cells , J Tiss Cul Meth 14 , 235 , 1992
Hamster Hamster, Syrian golden (strain CR:RGH)	Tracheal	Trypsin : 0.1%	Ham's F-12	McDowell, E. and et al. Differentiation of Tracheal Mucociliary Epithelium in Primary Cell Culture Recapitulates Normal Fetal Development and Regeneration Following Injury in Hamsters , Am J Pathol 129 , 511 , 1987
Hamster Hamster (strain 1516 EHS and Lakeview), 8-12 weeks	Tracheal	Trypsin : 0.05%	Medium 199	Lee, T. , Wu, R. , Brody, A. , Barrett, J. and Nettesheim, P. Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture , Exp Lung Res 6 , 27 , 1984
Hamster Hamster, Syrian gold, male, 100-120 g	Tracheal	Pronase : 0.1%	MEM with Hepes, CMF	Niles,R. , Kim, K.C. , Hyman, B. , Christensen, T. , Wasano, K. and Brody, J. Characterization Of Extended Primary And Secondary Cultures Of Hamster Tracheal Epithelial Cells , In Vitro Cell Dev Biol 24 , 457 , 1988
Hamster Hamster, Syrian gold, male, 6 weeks- 4 months	Tracheal	Trypsin : 0.25%	PBS with EDTA	Goldman, W.E. and Baseman, J.B. Selective Isolation and Culture of a Proliferating Epithelial Cell Population from the Hamster Trachea , In Vitro 16 , 313 , 1980
Human Human	Epithelial	Deoxyribonuclease I : 0.01%	HEPES with 5.9mM Glucose,5mM DTT	Widdicombe, J.H. , Coleman, D.L. , Finkbeiner, W.E. and Tuet, I.K. Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa , J Appl Physiol 58 , 1729 , 1985
Human Human	Gastrointestinal epithelial	Collagenase Type 1 : 0.2%	DMEM/F12	VanDussen, K. , Marinshaw, J. , Shaikh, N. , Miyoshi, H. , Moon, C. , Tarr, P. , Ciorba, M. and Stappenbeck, T. Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays. , Gut 64 , 911-20 , 2015
Human Human	Epithelial Sweat gland	Collagenase Type 2 : 0.2%	(see reference)	Wood, L. and Neufeld, E. A Cystic Fibrosis Phenotype in Cells Cultured from Sweat Gland Secretory Coil. Altered Kinetics of 36Cl Efflux , J Biol Chem 265 , 12796 , 1990
Human Human, infant and neonate	Epithelial Prostate	Trypsin : 0.1%	HBSS	Lechner, J. , Babcock, M. , Marnell, M. , Narayan, K. and Kaighn, M. Normal Human Prostate Epithelial Cell Cultures , Methods Cell Biol 21 , 195 , 1980
Human Human	Epithelial	Protease Type XIV : 0.1%	Eagle's MEM	Yankaskas, J. , Cotton, C. , Knowles, M. , Gatzy, J. and Boucher, R. Culture of Human Nasal Epithelial Cells on Collagen Matrix Supports , Am Rev Respir Dis 132 , 1281 , 1985
Human Human (also bovine)	Endometrial epithelial	Trypsin	DMEM/Ham's F-12	Munson, L. , Chandler, S. and Schlafer, D. Cultivation of Bovine Fetal and Adult Endometrial Epithelial Cells , J Tiss Cul Meth 11 (3) , 129 , 1988
Human Human	Epithelial	Trypsin : 0.05%	DMEM	Sabatini, L. , Allen-Hoffmann, B, Warner, T. and Azen, E. Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands , In Vitro Cell Dev Biol 27A , 939 , 1991
Human Human	Epithelial	Trypsin : 0.2%	MEM, PBS	Robinson, C. and Wu, R. Culture of Conducting Airway Epithelial Cells in Serum-Free Medium , J Tiss Cul Meth 13 , 95 , 1991
Human Human	Gastric	Collagenase Type 4 : 0.01%	F-12 medium	Sarosiek, J, Marshall, B, Peura, D, Guerrant, L, McCallum, R. and Little, C The Isolation and Maintenance of Human Gastric Epithelial Cells in Primary Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:10.1 , 1995
Human Human	Human tracheal epithelium	Protease Type XIV : 0.04%	DMEM/F12	Widdicombe JH, Sachs LA, Morrow JL. and Finkbeiner WE. Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function , Biotechniques 39(2) , 249 , 2005
Human Human	Corneal limbal epithelial sheet	Neutral Protease : 5%	see reference	Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R. and Tseng SC Novel enzymatic isolation of an entire viable human limbal epithelial sheet , Inv Ophthal Visual Sci 44(10) , 4275 , 2003
Human Human	Nasal polyp epithelial	Neutral Protease : .004% Trypsin : 0.1%	see reference	Halbert CL, Alexander IE, Wolgamot GM, Miller AD. Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells , J Virol 69 , 1473-9 , 1995
Human Human	Colonic epithelial	Collagenase Neutral Protease : 0.3% Deoxyribonuclease I : 0.05%	RPMI 1640	Fukushima, K. and Fiocchi, C. Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. , Am J Physiol Gastrointest Liver Physiol 286 , G804-13 , 2004
Human Human	Intestinal epithelial	Collagenase Type 4 : 72.5 u/ml	HBSS	Fahlgren, A. , Hammarstrom, S. , Danielsson, A. and Hammarstrom, M. Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis. , Clin Exp Immunol 131 , 90 , 2003
Human Human	Epithelial	Collagenase : 2.0%	DMEM/Ham's F-12	Emerman, J. and Wilkinson, D. Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples , In Vitro Cell Dev Biol 26 , 1186 , 1990
Human Human	Gastric epithelial cells	Collagenase Type 2 : 200 u/ml Neutral Protease : 1.2 u/ml Soybean Trypsin Inhibitor : 0.125%	L-15	Smoot, D. , Sewchand, J. , Young, K. , Desbrodes, B. , Allen, C. and Naab, T. A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells , Meth Cell Sci 22 , 133 , 2000
Human Human	Epithelial	Pronase : 0.1%	PBS	Gruenert, D.C. , Basbaum, C.B. and Widdicombe, J.H. Long-Term Culture of Normal and Cystic Fibrosis Epithelial Cells Grown Under Serum-Free Conditions , In Vitro Cell Dev Biol 26 , 411 , 1990
Human Human	Oral mucosal epithelial	Collagenase Type 1 : 0.05%	DMEM/F12	Hsueh, Y. , Huang, S. , Lai, J. , Ma, S. , Chen, H. , Wu, S. , Wang, T. , Sun, C. , Ma, K. , Chen, J. , Lai, C. and Ma, D. Preservation of Epithelial Progenitor Cells from Collagenase-Digested Oral Mucosa During Ex Vivo Cultivation. , Sci Rep 6 , 36266 , 2016
Human Human, women, 27-49 years	Epithelial Ovary	Trypsin : 0.125%	HBSS, CMF	Auersperg, N. , Siemens, C.H. and Myrdal, S.E. Human Ovarian Surface Epithelium In Primary Culture , In Vitro 20 , 743 , 1984
Human Human	Epithelial	Collagenase Type 4 : 0.1% Neutral Protease : 0.1%	PBS	Gottipamula, S. , Saraswat, S. and Sridhar, K. Comparative Study of Isolation, Expansion and Characterization of Epithelial Cells. , Cytotherapy 19 , 263-271 , 2017
Mouse Mouse	Tracheal epithelial	Papain : 50 u/ml	See reference	Pardo-Saganta, A. , Tata, P. , Law, B. , Saez, B. , Chow, R. , Prabhu, M. , Gridley, T. and Rajagopal, J. Parent Stem Cells Can Serve as Niches for Their Daughter Cells. , Nature 523 , 597-601 , 2015
Mouse Mouse, (BALB/c), male, 3-5 months	Epithelial Submandibular salivary gland	Collagenase Type 3 : 0.16% , 1:1 v/v	DMEM with 15 mM HEPES	Durban, E.M. Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix , In Vitro Cell Dev Biol 26 , 33 , 1990
Mouse Mouse	Epithelial	Collagenase : 0.025% Neutral Protease : 0.25%	DMEM	Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E. Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. , Methods Enzymol 446 , 77-106 , 2008
Mouse Mouse	Esophageal	Trypsin : 0.25%	PBS, CMF	Katayama, M. and Kan, M. Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation , In Vitro Cell Dev Biol 27 , 533 , 1991
Mouse Mouse	Alveolar epithelial	Neutral Protease : 0.1% Deoxyribonuclease I : 0.01%	DMEM	Sun, F. , Xiao, G. and Qu, Z. Isolation of Murine Alveolar Type II Epithelial Cells. , Bio-protocol 7 , 2017
Mouse Mouse, C3H, 6-8 weeks	Epithelial	Collagenase : 0.10%	DMEM	Lillehaug, J. , Mondal, S. and Heidelberger, C. Establishment of Epithelial Cell Lines from Mouse Regenerating Liver , In Vitro 15 , 910 , 1979
Mouse Mouse, 8-12 week	Nasal epithelium	Papain : 20 u/ml Deoxyribonuclease I : 0.005%	EBSS	Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S. Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. , Sci. Adv. 6 , 2020
Mouse Mouse (BALB/cfC3H)	Mammary tumors Epithelial	Collagenase : 1.0%	HBSS	Yang, J. , Guzman, R. , Richards, J. and Nandi, S. Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels , In Vitro 16 , 502 , 1980
Mouse Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant	Epithelial Submandibular gland	Collagenase Type 3 : 0.1%	HBSS	Yang, J. , Flynn, D. , Larson, L. and Hamamoto, S. Growth in Primary Culture of Mouse Submandibular Epithelial Cells , In Vitro 18 , 435 , 1982
Mouse Mouse, female	Epithelial	Pepsin : 0.1% and 0.05% Pepsin : 0.1%	HBSS with 0.2% EDTA, CMF	Riser, M. , Huff, B. and Medina, D. Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells , In Vitro 19 , 730 , 1983
Mouse Mouse, mature, female, 6-8-wk-old	Uterine	Trypsin : 0.2% Trypsin : 0.2%	HBSS	Ghosh, D. , Danielson, K. , Alston, J. and Heyner, S. Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes , In Vitro Cell Dev Biol 27A , 713 , 1991
Mouse Mouse, female, 18 - 20 days (also 20 - 22 days)	Uterine	Trypsin : 0.25%	HBSS	Fukamachi, H. and McLachlan, J. Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum , In Vitro Cell Dev Biol 27A , 907 , 1991
Mouse Mouse, fetal 12-13 day	Intestinal mesenchymal and epithelial	Collagenase : 0.03%	DMEM	Simon-Assmann, P. and Kedinger, M Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Mouse Mouse	Submandibular salivary	Collagenase Type 2 or 3 : 0.16%	DMEM	Durban, E. Submandibular Salivary Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:2.1 , 1995
Mouse Mouse, 6-8 week	Lamina propria mononuclear cells	Collagenase Type 2 : 0.015% Deoxyribonuclease I : 0.01%	RPMI	Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M. Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA , J Immunol 168(1) , 411 , 2002
Mouse Mouse, female	Salivary gland epithelial	Collagenase Type 1 : 750 u/ml Hyaluronidase : 500 u/ml	DMEM/F12	Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y. Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis , Am J Pathol 155 , 173-81 , 1999
Mouse Mouse, 11 week	Epithelial	Collagenase Type 3 : 25 u/ml Hyaluronidase : 0.1% Protease XIV : 0.05% Deoxyribonuclease I : 0.04%	DMEM/F12	Mueller, S. , Clark, J. , Myers, P. and Korach, K. Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. , Endocrinology 143 , 2357 , 2002
Mouse Mouse, male, 8-16 week	Renal tubular epithelial	Collagenase : 200 u/ml Soybean Trypsin Inhibitor : see reference	HBSS	Breggia, A. and Himmelfarb, J. Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. , Oxid Med Cell Longev 1 , 33 , 2008
Mouse Mouse, adult	Cilary epithelial	PDS kit : per instructions	EBSS	Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J. Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. , Curr Protoc Stem Cell Biol Chapter 1 , Unit 1H.4 , 2011
Porcine Porcine	Trachea	Neutral Protease : 0.2%	HBSS	De Buysscher, E. , Kennedy, J. and Mendicino, J. Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis , In Vitro 20 , 433 , 1984
Porcine Porcine, 5-60 kg	Retinal pigment epithelial cells	Collagenase : 2%	DMEM	Wiencke, A. , Kiilgaard, J. , Nicolini, J. , Bundgaard, M. , Ropke, C. and La Cour, M. Growth of Cultured Porcine Retinal Pigment Epithelial Cells , Acta Opthalmol Scand 81(2) , 170 , 2003
Rabbit Rabbit, New Zealand white, male, 4-5lb.	Gastric Parietal and chief	Collagenase Type 2 : 0.08%	Sodium phosphate buffer	Chew, C.S. and Brown,M.R. Release of Intracellular Ca2+ and Elevation of Inositol Triphosphate by Secretagogues in Parietal and Chief Cells Isolated from Rabbit Gastric Mucosa , Biochim Biophys Acta 888 , 116 , 1986
Rabbit Rabbit, fetal	Epithelial Gastric	Collagenase Type 3 : 0.10%	HBSS	Logsdon, C.D. , Bisbee, C.A. , Rutten, M.J. and Machen, T.E. Fetal Rabbit Gastric Epithelial Cells Cultured on Floating Collagen Gel , In Vitro 18 , 233 , 1981
Rabbit Rabbit, New Zealand white estrous, female, 4-5 months	Mesothelial and surface epithelial Ovaries	Trypsin : 0.125%-0.5%	Medium 199	Nicosia, S. , Johnson, J. and Streibel, E. Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium) , Int J Gynecol Pathol 3 , 348 , 1984
Rabbit Rabbit, New Zealand white, adult	Colon	Neutral Protease : 0.3%	PBS	Vidrich, A. , Racindranath, R. , Farsi, K. and Targan, S. A Method for the Rapid Establishment of Normal Adult Mammalian Colonic Epithelial Cell Cultures , In Vitro Cell Dev Biol 24 (3) , 188 , 1988
Rat Rat, Fisher 344, male, 8 wks old, 250-300 g	Tracheal epithelial	Pronase : 0.5%	DMEM	Chang, L. , Wu, R. and Nettesheim, P. Morphological Changes in Rat Tracheal Cells During The Adaptive and Early Growth Phase in Primary Cell Culture , J Cell Sci 74 , 283 , 1985
Rat Rat, SD, 200 g	Colon	Deoxyribonuclease I : 10 µg/ml	(see reference)	Yassin, R. , Clearfield, H. , Katz, S. and Murthy, S. Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro , Peptides 12 , 63 , 1991
Rat Rat, SD, adult, male, 8-10 weeks old	Seminiferous tubules	Trypsin : 0.05%	Krebs-Ringer bicarbonate buffer (see reference)	Abou-Haila, A. and Tulsiani, D. Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization , Biol Proced Online 3 (1) , 35 , 2001
Rat Rat, fetal, 14-15 day	Intestinal mesenchymal and epithelial	Collagenase : 0.03%	DMEM	Simon-Assmann, P. and Kedinger, M Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Rat Rat, Wistar, neonatal	Epithelial	Trypsin : 0.1%	HBSS	Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A. Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium , In Vitro Cell Dev Biol 27A , 625 , 1991
Rat Rat (ACI/NMs X BUF/Mna) F1, male, 28 months Rat (ACI/MNs) male, 8 weeks	Epthelial	Collagenase Type 3 : 0.1%	Eagle's MEM Serum-free	Masuda, A. , Ohtsuka, K. and Matsuyama, M. Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus , In Vitro Cell Dev Biol 26 , 713 , 1990
Rat Rat, Fischer, adult, 200-250 g	Epithelial	Hyaluronidase : 0.0075%	KCl-NaCl HEPES Buffer	Williams, G. and Gunn, J. Long-Term Culture of Adult Rat Liver Epithelial Cells , Exp Cell Res 89 , 139 , 1974
Rat Rat, Fischer, 10 day	Epithelial-like	Trypsin : 0.25%	PBS	Williams, G. , Weisburger, E. and Weisburger, J. Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver , Exp Cell Res 69 , 106 , 1971
Rat Rodent, various (see reference)	Epithelial	Trypsin : 0.2%	MEM, PBS	Robinson, C. and Wu, R. Culture of Conducting Airway Epithelial Cells in Serum-Free Medium , J Tiss Cul Meth 13 , 95 , 1991
Rat Rat, SD, female	Epithelial, cancer and tumor	Collagenase : 0.1%	Eagles's MEM	Cohen, L. Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma , In Vitro 18 , 565 , 1982
Rat Rat, 4-14 week	Retinal pigment epithelial cells	Collagenase Type 1 : 65 u/ml Hyaluronidase : 220 u/ml	CF Hanks with EDTA	Wang N, Koutz CA. and Anderson RE. A method for the isolation of retinal pigment epithelial cells from adult rats , Inv Ophthal Visual Sci 34(1) , 101 , 1993
Rat Rat, Wistar, 12 day	Epithelial	Trypsin : 0.05%	HBSS, CMF	Malan-Shibley, L. and Iype, P. Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels , Exp Cell Res 131 , 363 , 1981
Rat Rat, male	Alveolar epithelial	Elastase : 40 u/ml	DMEM	Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M. Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair , J Cell Sci 112 , 243 , 1999
Rat Rat, SD, male, 150 - 250 g	Epithelial Stomach	Pronase : 0.15%	Medium 199	Dial, E. , Kao, Y. and Lichtenberger, L. Effects of 16,16-Dimethyl Prostaglandin E2 On Glycoprotein And Lipid Synthesis of Gastric Epithelial Cells Grown in a Primary Culture , In Vitro Cell Dev Biol 27 , 39 , 1991
Rat Rat, 6 day old	Rat intestinal epithelial	Neutral Protease : 0.01% Collagenase : 300 u/ml	DMEM	Evans GS, Flint N, Somers AS, Eyden B. and Potten CS. The development of a method for the preparation of rat intestinal epithelial cell primary cultures , J Cell Sci 101 , 219 , 1992
Rat Rat, Fischer, male, 4-6 weeks	Epithelial Esophagus	Hyaluronidase : 0.1%	HEPES BSS	Babcock, M. , Marino, M. , Gunning, W. and Stoner, G. Clonal Growth and Serial Propagation of Rat Esophageal Epithelial Cells , In Vitro 19 , 403 , 1983
Rat Rat, 6-8 day	Retinal pigment epithelial	Neutral Protease : 2%	DMEM	Chang CW, Roque RS, Defoe DM. and Caldwell RB. An improved method for isolation and culture of pigment epithelial cells from rat retina , Curr Eye Res 10(11) , 1081 , 1991
Rat Rat, Fischer, Lewis and SD, male, 10-18 months	Epithelial	Trypsin : 0.05%	HBSS CMF	Herring, A. , Raychaudhuri, R. , Kelley, S. and Iybe, P. Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats , In Vitro 19 , 576 , 1983
Rat Rat, embryonic	Tracheal epithelial	Collagenase Type 4 : 0.05% Deoxyribonuclease I Neutral Protease	DMEM/F12	Shannon JM, Gebb SA. and Nielsen LD Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture , Development 126 , 1675 , 1999
Rat Rat, adult (also hamster)	Interlobular duct fragments	Papain	DMEM/Ham's F-12	Heimann, T. and Githens, S. Rat Pancreatic Duct Epithelium Cultured on a Porous Support Coated with Extracellular Matrix , Pancreas 6 (5) , 514 , 1991
Rat Rat	Mammary epithelial	Collagenase Type 3 : 0.35%	HBSS	Mei, N. , McDaniel, L. , Dobrovolsky, V. , Guo, X. , Shaddock, J. , Mittelstaedt, R. , Azuma, M. , Shelton, S. , McGarrity, L. , Doerge, D. and Heflich, R. The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats. , Toxicol Sci 115 , 412 , 2010
Rat Rat, SD, adult, 90-120 day old, 350-450 g	Epididymal epithelial	Collagenase Type 2 : 0.1%	HBSS	Klinefelter, G. A Novel System for the Co-Culture of Epididymal Epithelial Cells and Sperm From Adult Rats , J Tiss Cul Meth 14 , 195 , 1992