Intestine

Species	Cells	Enzyme(s)	Medium	Reference
Canine Dog, adult	Intestinal L-cells	Collagenase Type 1 : 75 u/ml	HBSS	Damholt AB, Buchan AM, Kofod H. Glucagon-like-peptide-1 secretion from canine L-cells is increased by glucose-dependent-insulinotropic peptide but unaffected by glucose , Endocrinology 139 , 2085-91 , 1998
Human Human	Microvessel endothelial	Collagenase Type 2 : 0.2%	HBSS	Kessler, S. , Rho, H. , West, G. , Fiocchi, C. , Drazba, J. and de la Motte, C. Hyaluronan (HA) Deposition Precedes and Promotes Leukocyte Recruitment in Intestinal Inflammation. , Clin Transl Sci 1 , 57-61 , 2008
Human Human	Lymphocytes	CLSPA : 0.05% Deoxyribonuclease I : 0.00075%	RPMI-1640	Trapecar, M. , Khan, S. , Roan, N. , Chen, T. , Telwatte, S. , Deswal, M. , Pao, M. , Somsouk, M. , Deeks, S. , Hunt, P. , Yukl, S. and Sanjabi, S. An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies. , AIDS Res Hum Retroviruses 33 , S31-S39 , 2017
Human Human	Lamina propria mononuclear cells	Collagenase : 0.02%	HBSS	Kanai Takanori, Totsuka Teruji, Uraushihara Koji, Makita Shin, Nakamura Tetsuya, Koganei Kazutaka, Fukushima Tsuneo, Akiba Hisaya, Yagita Hideo, Okumura Ko, Machida Utako, Iwai Hideyuki, Azuma Miyuki, Chen Lieping, Watanabe Mamoru. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation , J Immunol 171 , 4156-63 , 2003
Human Human	Mucosal mononuclear cells	Collagenase Type 3 : 0.01% Deoxyribonuclease I : 0.01% Soybean Trypsin Inhibitor : 0.01%	RPMI	Stallmach A, Schaffer F, Hoffmann S, Weber S, Muller-Molaian I, Schneider T, Kohne G, Ecker KW, Feifel G, Zeitz M. Increased state of activation of CD4 positive T cells and elevated interferon gamma production in pouchitis , Gut 43 , 499-505 , 1998
Human Human	Intestinal epithelial	Collagenase Type 4 : 72.5 u/ml	HBSS	Fahlgren, A. , Hammarstrom, S. , Danielsson, A. and Hammarstrom, M. Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis. , Clin Exp Immunol 131 , 90 , 2003
Human Human	Lamina propria lymphocytes	Collagenase : 20 u/ml	Medium	Ebert, Ellen. CD2 Activation of Human Lamina Propria Lymphocytes Reduces CD3 Responsiveness. , Immunology 117 , 71-7 , 2006
Mouse Mouse	Lymphocytes	Collagenase Type 1 : 100 u/ml	RPMI-1640	Beura, L. , Anderson, K. , Schenkel, J. , Locquiao, J. , Fraser, K. , Vezys, V. , Pepper, M. and Masopust, D. Lymphocytic Choriomeningitis Virus Persistence Promotes Effector-Like Memory Differentiation and Enhances Mucosal T cell Distribution. , J Leukoc Biol 97 , 217 , 2015
Mouse Mouse, embryonic	Crypt	Collagenase Type 1 : 200 u/ml Hyaluronidase : 100u/ml	DMEM	Ren HJ, Zhang CL, Liu RD, Li N, Li XG, Xue HK, Guo Y, Wang ZQ, Cui J. and Ming L Primary Cultures of Mouse Small Intestinal Epithelial Cells using the Dissociating Enzyme Type I Collagenase and Hyaluronidase. , Braz J Med Biol Res 50 , e5831 , 2017
Mouse Mouse, 7 week	Interstitial cells	Collagenase : 0.04% Trypsin : 0.02%	see reference	Goto Kazunori, Matsuoka Satoshi, Noma Akinori. Two types of spontaneous depolarizations in the interstitial cells freshly prepared from the murine small intestine , J Physiol 559 , 411-22 , 2004
Mouse Mouse	Lyphocytes	Collagenase Type 4 : 0.05% Neutral Protease : 0.1% Deoxyribonuclease I : 200 ug/ml	RPMI 1640	Ruiz, V. , Sachdev, M. , Zhang, S. , Wen, S. and Moss, S. Isolating, Immunophenotyping and Ex vivo Stimulation of CD4+ and CD8+ Gastric Lymphocytes During Murine Helicobacter Pylori Infection. , J Immunol Methods 384 , 157-63 , 2012
Mouse Mouse, F14	Intestinal mesenchymal	Collagenase Type 2 : 0.03%	HBSS	Sakagami Y, Inaguma Y, Sakakura T, Nishizuka Y. Intestine-like remodeling of adult mouse glandular stomach by implanting of fetal intestinal mesenchyme , Cancer Res 44 , 5845-9 , 1984
Mouse Mouse, 2-3 month	Enteric neural stem cells	Collagenase : 0.11% Neutral Protease : 0.1% Deoxyribonuclease I : 50 u/ml	M199	Becker, L. , Kulkarni, S. , Tiwari, G. , Micci, M. and Pasricha, P. Divergent Fate and Origin of Neurosphere-Like Bodies from Different Layers of the Gut. , Am J Physiol Gastrointest Liver Physiol 302 , G958-65 , 2012
Mouse Mouse, 0-15 day	Interstitial cells of Cajal	Collagenase Type 2 : 0.13%	Hanks	Lee Young Mee, Kim Byung Joo, Kim Hyun Jin, Yang Dong Ki, Zhu Mei Hong, Lee Kyu Pil, So Insuk, Kim Ki Whan. TRPC5 as a candidate for the nonselective cation channel activated by muscarinic stimulation in murine stomach , Am J Physiol/Gastro 284 , G604-16 , 2003
Mouse	Enteric neurons	Collagenase Type 4 : 0.1% Trypsin : 0.05% Deoxyribonuclease I : 400 u/ml	DMEM/F12	Zhang, Y. and Hu, W. Mouse Enteric Neuronal Cell Culture. , Methods Mol Biol 1078 , 55-63 , 2013
Mouse Mouse, 9-13 day	Interstitial cells of Cajal	Collagenase Type 2 : 0.13%	HBSS	Ordag Tamas, Redelman Doug, Miller Lisa J, Horvath Viktor J, Zhong Qiao, Almeida-Porada Graca, Zanjani Esmail D, Horowitz Burton, Sanders Kenton M. Purification of interstitial cells of Cajal by fluorescence-activated cell sorting , Am J Physiol Cell Physiol 286 , C448-56 , 2004
Mouse Mouse	Intestinal organoids	Collagenase Type 4 : 500 u/ml Deoxyribonuclease I : 15 u/ml	Basal Medium	O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S. Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. , Bio Protoc 6 , 2016
Mouse Mouse	Lamina propria lymphocytes	Collagenase Type 4 : 300 u/ml	PBS	Wu Y, Wang X, Csencsits KL, Haddad A, Walters N, Pascual DW. M cell-targeted DNA vaccination , Proc Natl Acad Sci U S A 98 , 9318-23 , 2001
Mouse Mouse	Lamina propria lymphocytes	Collagenase Type 4 : 0.02% Deoxyribonuclease I : 0.01%	HBSS	Emgard, J. , Kammoun, H. , Garcia-Cassani, B. , Chesne, J. , Parigi, S. , Jacob, J. , Cheng, H, Evren, E. , Das, S. , Czarnewski, P. , Sleiers, N. Melo-Gonzalez, F. , Kvedaraite, E. Svensson, M. and Scandella, E. Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. , Immunity 48 , 120-132.e8 , 2018
Mouse Mouse	Intestinal organoids	Collagenase Type 1 : 800 u/ml Neutral Protease : 0.013%	DMEM	Barthel, E. , Speer, A. , Levin, D. , Sala, F. , Hou, X. , Torashima, Y. , Wigfall, C. and Grikscheit, T. Tissue Engineering of the Intestine in a Murine Model. , J Vis Exp 70 , e4279 , 2012
Mouse Mouse, P4	Enteric neural crest progenitors	Neutral Protease : 0.5% Collagenase animal free : 0.05%	DMEM/F12	Hotta, R. , Stamp, L. , Foong, J. , McConnell, S. , Bergner, A. , Anderson, R. , Enomoto, H. , Newgreen, D. , Obermayr, F. , Furness, J. and Young, H. Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon. , J Clin Invest 123 , 1182 , 2013
Mouse Mouse	Lamina propria lymphocytes	Collagenase Type 1 : 0.3% Deoxyribonuclease I : 0.01%	RPMI 1640	Ito, Y. , Kanai, T. , Totsuka, T. , Okamoto, R. , Tsuchiya, K. , Nemoto, Y. , Yoshioka, A. , Tomita, T. , Nagaishi, T. , Sakamoto, N. , Sakanishi, T. , Okumura, K. , Yagita, H. and Watanabe, M. Blockade of NKG2D Signaling Prevents the Development of Murine CD4+ T Cell-Mediated Colitis. , Am J Physiol Gastrointest Liver Physiol 294 , G199 , 2008
Mouse Mouse	Intestinal mononuclear	Collagenase Type 4 : 0.1%	RPMI 1640	Forbes, E. , Groschwitz, K. , Abonia, J.P. , Brandt, E. , Cohen, E. , Blanchard, C. , Ahrens, R. , Seidu, L. , McKenzie, A. , Strait, R. , Finkelman, F. , Foster, P. , Matthaei, K. , Rothenberg, M. and Hogan, S. IL-9- and Mast Cell-Mediated Intestinal Permeability Predisposes to Oral Antigen Hypersensitivity. , J Exp Med 205 , 897 , 2008
Mouse Mouse, neonatal and adult	Myenteric plexus	Collagenase Type 4 : 0.025-0.1% Papain : 10 u/ml Deoxyribonuclease I : 100 u/ml	HBSS	Joseph, N. , He, S. , Quintana, E. , Kim, Y. , Nunez, G. and Morrison, S. Enteric Glia are Multipotent in Culture but Primarily Form Glia in the Adult Rodent Gut. , J Clin Invest 121 , 3398 , 2011
Mouse Mouse	Dendritic, macrophages	Collagenase : 0.15% Deoxyribonuclease I : 0.004%	HBSS	Geem, D. , Medina-Contreras, O. , Kim, W. , Huang, C. and Denning T. Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine. , J Vis Exp 63 , e4040 , 2012
Mouse Mouse, Mastomys natalensis	Enterochromaffin cells	Collagenase : 0.025% Pronase E : 0.07%	HBSS	Kidd, M. , Modlin, I. , Eick, G. and Champaneria, M. Isolation, Functional Characterization, and Transcriptome of Mastomys Ileal Enterochromaffin Cells. , Am J Physiol Gastrointest Liver Physiol 291 , G778-91 , 2006
Mouse Mouse	Colon tumor organoids	Collagenase Type 4 : 200 u/ml Neutral Protease : 0.01%	DMEM	Xue, X. and Shah, Y. In Vitro Organoid Culture of Primary Mouse Colon Tumors. , J Vis Exp , e50210 , 2013
Mouse Mouse	Lamina propria mononuclear cells	Collagenase Type 3 : 400 u/ml Deoxyribonuclease I : 0.001%	HBSS	Seo, S. , Kuffa, P. , Kitamoto, S. , Nagao-Kitamoto, H. , Rousseau, J. , Kim, Y. , Nunez, G. and Kamada, N. Intestinal Macrophages Arising from CCR2(+) Monocytes Control Pathogen Infection by Activating Innate Lymphoid Cells. , Nat Commun 6 , 8010 , 2015
Rat Rat, male, 18-24 day	Intestinal epithelial	Collagenase Type 1 : 0.1%	DMEM	Quaroni A, Wands J, Trelstad RL, Isselbacher KJ. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria , J Cell Biol 80 , 248-65 , 1979
Rat Rat, postnatal, adult	Gut neural crest stem	Collagenase Type 4 : 0.1% Trypsin : 0.025%	HBSS	Kruger, G. , Mosher, J. , Bixby, S. , Joseph, N. , Iwashita, T. and Morrison, S. Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness. , Neuron 35 , 657 , 2002
Rat Rat, SD, embryonic	Sciatic nerve and gut neural crest stem	Collagenase Type 4 : 0.025% Trypsin : 0.005% Deoxyribonuclease I : 0.05%	HBSS	Bixby, S. , Kruger, G. , Mosher, J. , Joseph, N. and Morrison, S. Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity. , Neuron 35 , 643 , 2002