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Tissue References Search Tool

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Pancreas
Mouse
Mouse, 3-4 week, 20-24 g
Huang, H. , Xie, Q. , Kang, M. , Zhang, B. , Zhang, H. , Chen, J. , Zhai, C. , Yang, D. , Jiang, B. and Wu, Y.
Labeling Transplanted Mice Islet with Polyvinylpyrrolidone Coated Superparamagnetic Iron Oxide Nanoparticles for In Vivo Detection By Magnetic Resonance Imaging. ,
Nanotechnology
 20 , 365101 , 2009
Cells
Islets
Enzymes
Medium
RPMI 1540
Pancreas
Mouse
Mouse
Szot, G. , Koudria, P. and Bluestone, J.
Murine Pancreatic Islet Isolation. ,
J Vis Exp
 7 , 255 , 2007
Cells
Islets
Enzymes
Collagenase : 0.03-0.08%
Medium
RPMI 1640
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
File
10607.pdf (419.04 KB)
Pancreas
Mouse
Mouse, adult
Voronina, S. , Barrow, S. , Gerasimenko, O. , Petersen, O. and Tepikin, A.
Effects of Secretagogues and Bile Acids on Mitochondrial Membrane Potential of Pancreatic Acinar Cells: Comparison of Different Modes of Evaluating Delta Psi. ,
J Biol Chem
 279 , 27327 , 2004
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
see reference
Pancreas
Mouse
Mouse, 11-12 week
Astrof, S. , Crowley, D. , George, E. , Fukuda, T. , Sekiguchi, K. , Hanahan, D. and Hynes, R.
Direct Test of Potential Roles of EIIIA and EIIIB Alternatively Spliced Segments of Fibronectin in Physiological and Tumor Angiogenesis. ,
Mol Cell Biol
 24 , 8662-70 , 2004
Cells
Islets
Enzymes
Medium
RPMI
Pancreas
Mouse
Mouse
Wu Yulian, Han Bing, Luo Hongyu, Roduit Raphael, Salcedo TheodoraW, Moore PaulA, Zhang Jun, Wu Jiangping.
DcR3/TR6 effectively prevents islet primary nonfunction after transplantation ,
Diabetes
 52 , 2279-86 , 2003
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Mouse
Mouse
Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard.
The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells ,
J Cell Sci
 116 , 1463-9 , 2003
Cells
Pancreatic islet
Enzymes
Medium
HBSS
Pancreas
Mouse
Mouse, BALB/c, 6-8 week-old, either sex
Kurup, S. and Bhonde, R.
Analysis and Optimization of Nutritional Set-up for Murine Pancreatic Acinar Cells ,
JOP
 3 (1) , 8 , 2002
Cells
Acinar
Enzymes
Collagenase : 0.1%
Medium
Waymouth's MB
Pancreas
Mouse
Mouse, albino, 25 g
Fogarty, K. , Kidd, J. , Tuft, R. and Thorn, P.
A Bimodal Pattern of InsP(3)-Evoked Elementary Ca(2+) Signals in Pancreatic Acinar Cells. ,
Biophys J
 78 , 2298 , 2000
Cells
Acinar
Enzymes
CLSPA : see reference
Medium
see reference
Pancreas
Mouse
Mouse
Strowski M, Parmar R, Blake A, Schaeffer J.
Somatostatin inhibits insulin and glucagon secretion via two receptors subtypes: an in vitro study of pancreatic islets from somatostatin receptor 2 knockout mice ,
Endocrinology
 141 , 111-7 , 2000
Cells
Pancreatic islets
Enzymes
Medium
Gey's BSS
Pancreas
Mouse
Mouse
Koster, J. , Marshall, B. , Ensor, N. , Corbett, J. and Nichols, C.
Targeted Overativity of Cell KATP Channels Induces Profound Neonatal Diabetes ,
Cell
 100 , 645 , 2000
Cells
Islets
Enzymes
Medium
CF Medium
Pancreas
Mouse
Mouse
Toivola, D. , Ku, N. , Ghori, N. , Lowe, A. , Michie, S. and Omary, M.
Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury. ,
Exp Cell Res
 255 , 156 , 2000
Cells
Acinar cells and acini
Enzymes
Collagenase Type 1 : see reference
CLSPA : see reference
Medium
see reference
Pancreas
Mouse
Mouse
Githens, S.
Pancreatic Duct Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:12.1 , 1995
Cells
Duct
Enzymes
Papain : 25 u/ml
Medium
DMEM /F-12
Pancreas
Mouse
Mouse
Jauch, P. , Peterson, O. and Lauger, P.
Electrogenic Properties of the Na-Alanine Cotransporter in Acinar Cells ,
J Membr Biol
 94 , 99 , 1986
Cells
Acinar
Enzymes
Collagenase : 100 u/ml
Medium
HEPES
Pancreas
Mouse
Mouse (C57BL/6J-ob/ob), 9-12 wks, male
Dalpe-Scott, M. , Heick, H. and Begin-Heick, N.
Secretion in the Obese (ob/ob) Mouse. The Effect of Oxytetracycline on Insulin Release ,
Diabetes
 32 , 932 , 1983
Cells
Islets
Enzymes
Hyaluronidase : 0.5%
Medium
Kreb's Ringer bicarbonate buffer
Pancreas
Mouse
Mouse, male, 18-24 g
Burnham DB, Williams JA.
Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini ,
J Biol Chem
 257 , 10523-8 , 1982
Cells
Acinar
Enzymes
CLSPA : 70-90 u/ml
Medium
Krebs-Henseleit
Pancreas
Mouse
Mouse, 7-10 week
Yesil, P, Michel, M. , Chwalek, K. , Pedack, S. , Jany, C. , Ludwig, B. , Bornstein, S. and Lammert, E.
A New Collagenase Blend Increases the Number of Islets Isolated from Mouse Pancreas. ,
Islets
 1 , 185
Cells
Islets
Enzymes
Collagenase : see reference
Medium
DMEM
File
10614.pdf (854.46 KB)
Pancreas
Porcine
Porcine, juvenile
Van der Burg Michael P M, Graham John M.
Iodixanol Density Gradient Preparation in University of Wisconsin solution for porcine islet purification ,
ScientificWorldJournal
 3 , 1154-9 , 2003
Cells
Islets
Enzymes
Collagenase : see reference
Medium
Univ of Wisconsin solution
Pancreas
Porcine
Porcine, 3 month, 15-20 kg
Zhao, X. , Han, J. and Tang, C.
Primary Culture of Porcine Pancreatic Acinar Cells ,
JOP
 2 (2) , 78 , 2001
Cells
Acinar
Enzymes
Collagenase Type 3 : 200 u/ml
Medium
RPMI 1640
Pancreas
Porcine
Porcine, 1-3 year, 1.5-2.0 kg, either sex
Korbutt, G. , Elliott, J. , Ao, Z. , Smith, D. , Warnock, G. and Rajotte, R.
Large Scale Isolation, Growth, and Function of Porcine Neonatal Islet Cells ,
J Clin Invest
 97 (9) , 2119 , 1996
Cells
Islets
Enzymes
Collagenase : 0.25%
Medium
HBSS
Pancreas
Porcine
Porcine, female
Brandhorst, D. , Brandhorst, H. , Hering, B. , Federlin, K. and Bretzel, R.
Islet Isolation from the Pancreas of Large Mammals and Humans: 10 Years of Experience ,
Exp Clin Endocrinol
 103 , 3 , 1995
Cells
Islets
Enzymes
Collagenase : 0.1%
Medium
HBSS
Pancreas
Porcine
Porcine
Johnson, P. , van Suylichem, P. , Roberts, D. , Vos-Scheperkeuter, G. , White, S. , van Schlifgaarde, R. and London, N.
Design of a Simple, in vitro Method for Evaluation of the Efficiency of Crude Clostridium histolyticum Collagenase and its Components for Porcine Islet Isolation ,
Xenotransplantation
 2 , 165 , 1995
Cells
Islets
Enzymes
Collagenase : 0.1%-0.2%
Medium
HBSS
Pancreas
Porcine
Porcine, <2 year, 200-250 kg
Heiser, A. , Ulrichs, K. and Muller-Ruchholtz, W.
Isolation of Porcine Pancreatic Islets: Low Trypsin Activity During the Isolation Procedure Guarantees Reproducible High Islet Yields ,
J Clin Lab Anal
 8 , 407 , 1994
Cells
Islets
Enzymes
Collagenase : 0.1%
Medium
HBSS
Pancreas
Porcine
Porcine, 10-36 months, 200-300 kg
Ricordi, C. , Socci, C. , Davalli, A. , Staudacher, C. , Baro, P. , Vertova, A. , Sassi, I. , Gavazzi, F. , Pozza, G. and Di Carlo, V.
Isolation of the Elusive Pig Islet ,
Surgery
 107 (6) , 688 , 1990
Cells
Islets
Enzymes
Collagenase : 0.2%
Medium
HBSS
Pancreas
Porcine
Porcine
Iwatsuki, N. and Peterson, O.
Action of Tetraethylammonium on Calcium-Activated Potassim Channels in Pig Pancreatic Acinar Cells Studied by Patch-Clamp Single-Channel and Whole-Cell Current Recording ,
J Membr Biol
 86 , 139 , 1985
Cells
Acinar
Enzymes
Collagenase : 100 u/ml
Medium
Saline
Pancreas
Rabbit
Rabbit, New Zealand white, Male/Female, 2-3 Kg
Renckens, B. , Schrijen, J. , Swarts, H. , DePont, J. and Bonting, S.
Role of Calcium in Exocrine Pancreatic Secretion. IV. Calcium Movements in Isolated Acinar Cells of Rabbit Pancreas ,
Biochim Biophys Acta
 544 , 338 , 1978
Cells
Acinar
Enzymes
Hyaluronidase : 0.2%
Medium
Kreb's Ringer bicarbonate buffer
Pancreas
Rabbit
Rabbit, also hamster
Feldman, J. and Chapman, B.
Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique ,
Acta Diabetol
 12 , 208 , 1975
Cells
Islets
Enzymes
Collagenase Type 4 : 1.3% - 2.0%
Medium
HBSS
Pancreas
Rat
Rat, SD, adult
Li, J. , Zhou, R. , Bie, B. , Huang, N. , Guo, Y. , Chen, H. , Shi, M. , Yang, J. , Zhang, J. and Li, Z.
Emodin and Baicalein Inhibit Sodium Taurocholate-Induced Vacuole Formation in Pancreatic Acinar Cells. ,
World J Gastroenterol
 24 , 35-45 , 2018
Cells
Acinar
Enzymes
Medium
Culture media
File
11575.pdf (1.32 MB)
Pancreas
Rat
Rat
Williams, J.
Isolation of Rodent Pancreatic Acinar Cells and Acini by Collagenase Digestion ,
Pancreapedia
 , 2010
Cells
Acinar
Enzymes
CLSPA : 100 u/ml
Medium
DMEM
File
10889.pdf (899.26 KB)
Pancreas
Rat
Rat, Wistar, 300g
Verga Falzacappa, C. , Mangialardo, C. , Raffa, S. , Mancuso, A. , Piergrossi, P. , Moriggi, G. , Piro, S. , Stigliano, A. , Torrisi, M. , Brunetti, E. , Toscano, V. and Misiti, S.
The Thyroid Hormone T3 Improves Function and Survival of Rat Pancreatic Islets During In Vitro Culture. ,
Islets
 2 , 96 , 2010
Cells
Islets
Enzymes
Medium
CMRL 1066
File
10615.pdf (577.05 KB)
Pancreas
Rat
Rat, adult
Williams, J. , Huang, H. , Kover, K. , Moore, W. , Berkland, C. , Singh, M. , Smirnova, I. , MacGregor, R. and Stehno-Bittel, L.
Reduction of Diffusion Barriers in Isolated Rat Islets Improves Survival, but not Insulin Secretion or Transplantation Outcome. ,
Organogenesis
 6 , 115 , 2010
Cells
Islets
Enzymes
Collagenase Type 1 : 450 u/ml
Medium
Leibowitz L-15
File
10958.pdf (1010.04 KB)
Pancreas
Rat
Rat, male, 7-11 week
Getty-Kaushik, L. , Richard, A. , Deeney, J. , Shirihai, O. and Corkey, B.
The CB1 Antagonist Rimonabant Decreases Insulin Hypersecretion in Rat Pancreatic Islets ,
Obesity
 17 , 1856 , 2009
Cells
Islets
Enzymes
Collagenase Type 4 : see reference
Medium
RPMI 1640
Pancreas
Rat
Rat
MacGregor, R. , Williams, S. , Tong, P. , Kover, K. , Moore, W. and Stehno-Bittel, L.
Small Rat Islets are Superior to Large Islets in In Vitro Function and in Transplantation Outcomes. ,
Am J Physiol/Endo
 290 , E771 , 2006
Cells
Islets
Enzymes
Collagenase Type 1 : 450 u/ml
Medium
RPMI 1640
Pancreas
Rat
Rat, Wistar, male, 250-400g
Tian XH, Xue WJ, Ding XM, Pang XL, Teng Y, Tian PX. and Feng XS
Small intestinal submucosa improves islet survival and function during in vitro culture ,
World J Gastroenterol
 11 , 7378 , 2005
Cells
Pancreatic islets
Enzymes
Collagenase : 0.75%
Medium
RPMI 1640
Pancreas
Rat
Rat, 250-350 g
Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard.
The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells ,
J Cell Sci
 116 , 1463-9 , 2003
Cells
Pancreatic islet
Enzymes
Medium
HBSS
Pancreas
Rat
Rat, SD, male
Ji B, Kopin AS, Logsdon CD.
Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180 ,
J Biol Chem
 275 , 19115-20 , 2000
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.