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Tissue References Search Tool

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Endothelial
Human
Human
Gordon, P. , Sussman, I. and Hatcher, V.
Long-Term Culture of Human Endothelial Cells ,
In Vitro
 19 , 661 , 1983
Cells
Fibroblasts
Foreskin
Enzymes
Hyaluronidase : 0.10%
Medium
DMEM
Endothelial
Human
Human
Glassberg, M. , Bern, M. , Coughlin, S. , Haudenschild, C. , Hoyer, L. and Antoniades, H.
Cultured Endothelial Cells Derived from the Human Iliac Arteries ,
In Vitro
 18 , 859 , 1982
Cells
Iliac arteries
Enzymes
Collagenase : 0.25%
Medium
PBS w/Ca++, Mg++, & BSA
(see reference)
Endothelial
Human
Human, adult
Ashida, E. , Johnson, A. and Lipsky, P.
Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro ,
J Clin Invest
 67 , 1490 , 1981
Cells
Peripheral blood mononuclear
Monocytes
T cells
Endothelial
Enzymes
Collagenase : 0.25%
Medium
RPMI 1640
Endothelial
Human
Human
Sherer, G. , Fitzharris, T. , Faulk, W. and LeRoy, E.
Cultivation of Microvascular Endothelial Cells from Human Preputial Skin ,
In Vitro
 16 , 675 , 1980
Cells
Microvascular endothelial
Neonatal foreskins
Enzymes
Neutral Protease : at 1000 u/ml
Medium
Konigsberg's modification of HBSS
(See Reference)
Endothelial
Human
Human (adult and child)
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Endothelial
Human
Human
Jaffe, E.A. , Minick, C.R. , Adelman, B. , Becker, C.G. and Nachman, R.
Synthesis of Basement Membrane Collagen By Cultured Human Endothelial Cells ,
J Exp Med
 144 , 209 , 1976
Cells
Umbilical vein
Enzymes
Trypsin : 100 µg/ml
Medium
Tris-HCl,0.2 M
Endothelial
Human
Human
Gimbrone Jr.
Culture of Vascular Endothelium ,
Prog Hemost Thromb
 3 , 1 , 1976
Cells
Umbilical vein
Enzymes
Collagenase Type 1 : 125 u/ml
Medium
Dulbecco's PBS
Endothelial
Human
Human
Gimbrone, M.A. , Cotran, R.S. and Folkman, J.
Human Vascular Endothelial Cells in Culture: Growth and DNA Synthesis ,
J Cell Biol
 60 , 673 , 1974
Cells
Umbilical cord
Enzymes
Collagenase : 0.1%
Medium
Dulbecco's PBS
Endothelial
Human
Human
Jaffe, E. , Nachman, R. , Becker, C. and Minick, C.
Culture of Human Endothelial Cells Derived from Umbilical Veins. Identification by Morphologic and Immunologic Criteria ,
J Clin Invest
 52 , 2745 , 1973
Cells
Umbilical cord
Enzymes
Collagenase : 0.2%
Medium
Cord buffer
(See Reference)
Endothelial
Human
Human
Lewis, L.J. , Haok, J.C. , Maca, R.D. and Fry, G.L.
Replication of Human Endothelial Cells in Culture ,
Science
 181 , 452 , 1973
Cells
Umbilical cord
Enzymes
Trypsin NF 1:250 : 0.125%
Medium
Saline, normal
Endothelial
Human
Human
Fryer, D.G. , Birnbaum, G. and Luttrell, C.N.
Human Endothelium in Cell Culture ,
J Atheroscler Res
 6 , 151 , 1966
Cells
Umbilical cord
Enzymes
Medium
CMF solution
Endothelial
Mouse
Mouse, 4 week
Esteban, S. , Clemente, C. , Koziol, A. , Gonzalo, P. , Rius, C. , Martinez, F. , Linares, P. , Chaparro, M. , Urzainqui, A. , Andres, V. , Seiki, M. , Gisbert, J. and Arroyo, A.
Endothelial MT1-MMP Targeting Limits Intussusceptive Angiogenesis and Colitis via TSP1/Nitric Oxide Axis. ,
EMBO Mol Med
 12 , e10862 , 2020
Cells
Aortic endothelial
Enzymes
Elastase : 0.25%
Medium
DMEM
File
11726.pdf (5.65 MB)
Endothelial
Mouse
Mouse, 2 month
Sabbagh, M. and Nathans, J.
A Genome-wide View of the De-differentiation of Central Nervous System Endothelial Cells in Culture ,
Elife
 , 1 , 2020
Cells
Endothelial, CNS
Enzymes
PDS kit : with modifications
Stemxyme 1 : 0.1%
Medium
EC growth medium
File
11706.pdf (1.32 MB)
Endothelial
Mouse
Mouse
Luo, S. , Truong, A. and Makino, A.
Isolation of Mouse Coronary Endothelial Cells. ,
J Vis Exp
 , 2016
Cells
Coronary endothelial
Enzymes
Medium
Kreb's
Endothelial
Mouse
Mouse
Wang, J. , Sun, C. , Gerdes, N. , Liu, C. , Liao, M. , Liu, J. , Shi, M. , He, A. , Zhou, Y. , Sukhova, G. , Chen, H. , Cheng, X. , Kuzuya, M. , Murohara, T. , Zhang, J. , Cheng, X. , Jiang, M. , Shull, G. and Rogers, S.
Interleukin 18 Function in Atherosclerosis is Mediated by the Interleukin 18 Receptor and the Na-Cl Co-Transporter. ,
Nat Med
 21 , 820-6 , 2015
Cells
Endothelial lung
Enzymes
Medium
DMEM
File
11425.pdf (4.24 MB)
Endothelial
Mouse
Mouse
Xiao, L. , McCann, J. and Dudley, A.
Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
 , e53072 , 2015
Cells
Endothelial
Enzymes
Medium
DMEM
File
11553.pdf (1.84 MB)
Endothelial
Mouse
Mouse
Kochukov, M. , Balasubramanian, A. , Abramowitz, J. , Birnbaumer, L. and Marrelli, S.
Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery. ,
J Am Heart Assoc
 3 , 2014
Cells
Cerebral artery endothelial
Enzymes
Neutral Protease : 4 u/ml
Elastase : 1 u/ml
Collagenase Type 1 : 120 u/ml
Medium
DMEM
File
11522.pdf (1.43 MB)
Endothelial
Mouse
Mouse
Kazerounian, S. , Gerald, D. , Huang, M. , Chin, R, Udayakumar, D, Zheng, N. , O'Donnell, R. , Perruzzi, C. , Mangiante, L. , Pourat, J. , Phung, T. , Bravo-Nuevo, A. , Shechter, S. , McNamara, S. and Duhadaway, K.
RhoB Differentially Controls Akt Function in Tumor Cells and Stromal Endothelial Cells During Breast Tumorigenesis. ,
Cancer Res
 73 , 50 , 2013
Cells
Tumor endothelial
Enzymes
Medium
HBSS
File
11064.pdf (2.54 MB)
Endothelial
Mouse
Mouse, 8-14 week
Imoukhuede, P. and Popel, A.
Expression of VEGF Receptors on Endothelial Cells in Mouse Skeletal Muscle. ,
PLoS ONE
 7 , e44791 , 2012
Cells
Endothelial
Enzymes
Medium
HBSS
File
10956.pdf (1.1 MB)
Endothelial
Mouse
Mouse
Daneman, R. , Zhou, L. , Agalliu, D. , Cahoy, J. , Kaushal, A. and Barres, B.
The Mouse Blood-Brain Barrier Transcriptome: a New Resource for Understanding the Development and Function of Brain Endothelial Cells. ,
PLoS ONE
 5 , e13741 , 2010
Cells
Endothelial
Enzymes
Papain : 40 u/ml
Deoxyribonuclease I : 125 u/ml
Medium
DPBS
File
11485.pdf (2.98 MB)
Endothelial
Mouse
Mouse, 7-10 week
Follenzi, A. , Benten, D. , Novikoff, P. , Faulkner, L. , Raut, S. and Gupta, S.
Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice. ,
J Clin Invest
 118 , 935 , 2008
Cells
Liver endothelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Endothelial
Mouse
Mouse, 4 week
Kondo, S. , Scheef, E. , Sheibani, N. and Sorenson, C.
PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis. ,
Am J Physiol Cell Physiol
 292 , C2070 , 2007
Cells
Endothelial kidney
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, embryonic or yolk sac
Braren Rickmer, Hu Huiqing, Kim YungHae, Beggs HilaryE, Reichardt LouisF, Wang Rong.
Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation ,
J Cell Biol
 172 , 151-62 , 2006
Cells
Endothelial
Enzymes
Medium
PBS
Endothelial
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, 4 week
Su X, Sorenson CM. and Sheibani N.
Isolation and characterization of murine retinal endothelial cells ,
Mol Vis
 9 , 171 , 2003
Cells
Retinal endothelial cells
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, male
Izawa D, Tanaka T, Saito K, Ogihara H, Usui T, Kawamoto S, Matsubara K, Okubo K. and Miyasaka M
Expression profile of active genes in mouse lymph node high endothelial cells ,
Int Immunol
 11(12) , 1989 , 1999
Cells
Endothelial cells from lymph node
Enzymes
Medium
PBS
Endothelial
Mouse
Mouse, 2-4 week
Sapatino, B. , Welsh, C. , Smith, C. , Bebo, B. and Linticum, D.
Cloned Mouse Cerebrovascular Endothelial Cells That Maintain Their Differentiation Markers for Factor VIII, Low Density Lipoprotein, and Angiotensin-Converting Enzyme ,
In Vitro Cell Dev Biol
 29A , 923 , 1993
Cells
Cerebrovascular Endothelial
Enzymes
Medium
PBS
Endothelial
Porcine
Porcine, 6 month
Balaoing, L. , Post, A. , Lin, A. , Tseng, H. , Moake, J. and Grande-Allen, K.
Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation. ,
PLoS ONE
 10 , e0130749 , 2015
Cells
Valvular endothelial
Enzymes
Medium
PBS
File
11409.pdf (2.75 MB)
Endothelial
Porcine
Porcine
Gould, R. and Butcher, J.
Isolation of Valvular Endothelial Cells. ,
J Vis Exp
 46 , e2158 , 2010
Cells
Valvular endothelial
Enzymes
Collagenase Type 2 : 300-600 u/ml
Medium
DMEM
File
10900.pdf (786.22 KB)
Endothelial
Porcine
Porcine
(also bovine)
Nugent, H. and Edelman, E.
Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury ,
J Surg Res
 99 , 228 , 2001
Cells
Endothelial
Enzymes
Medium
DMEM w/ 10% calf serum
Endothelial
Porcine
Porcine
Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY. and Koh GY.
Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells ,
Circulation
 101(19) , 2317 , 2000
Cells
HUVEC, porcine pulmonary arterial endothelial cells
Enzymes
Medium
DMEM
Endothelial
Porcine
Porcine, 6-7 month
Hill-Kapturczak N, Kapturczak MH, Block ER, Patel JM, Malinski T, Madsen KM. and Tisher CC.
Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV ,
J Am Soc Nephrol
 10(3) , 481 , 1999
Cells
Porcine pulmonary endothelial
Enzymes
Medium
RPMI 1640
Endothelial
Porcine
Porcine
Shasby, S.
Endothelial Cells Grown On Permeable Membrane Supports ,
J Tiss Cul Meth
 14 , 247 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.25%
Medium
Medium 199
Endothelial
Porcine
Porcine
Vischer, P. and Buddecke, E.
Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells ,
Exp Cell Res
 158 , 15 , 1985
Cells
Endothelial
Enzymes
Trypsin : 0.05%
Medium
DMEM
Endothelial
Porcine
Porcine
Dickinson, E. and Slakey, L.
Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta ,
In Vitro
 18 , 63 , 1982
Cells
Endothelial
Aortic
Enzymes
Medium
Dulbecco-Vogt MEM w/o serum
Endothelial
Porcine
Porcine, 30-40 kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.