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Tissue References Search Tool

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Stem
Rat
Rat, adult
Palmer, T. , Markakis, E. , Willhoite, A. , Safar, F. and Gage, F.
Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS ,
J Neurosci
 19 , 8487 , 1999
Cells
Neural stem cells
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Stem
Rat
Rat
Ray, J. and Gage, F.
Neural Stem Cell Isolation, Characterization and Transplantation , Modern Techniques in Neuroscience Research , Johansson, H. and Windhorst, U. , Springer-Verlag New York , 339 , 1999
Cells
Central nervous system stem cells
Enzymes
Papain : 0.01%
Medium
DMEM/F-12
Stem
Rat
Rat (also mice)
Johansson, C. , Momma, S. , Clarke, D. , Risling, M. , Lendahl, U. and Frisen, J
Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System ,
Cell
 96 , 25 , 1999
Cells
Spinal cord
Enzymes
Trypsin : 0.133%
Medium
HBSS and PIPES
Stem
Rat
Rat, SD
Morrison, S. , White, P. , Zock, C. and Anderson, D.
Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells ,
Cell
 96 , 737 , 1999
Cells
Sciatic nerves
Enzymes
Trypsin : 0.025%
Medium
L-15 medium
(see reference)
File
1099.pdf (350.54 KB)
Stem
Rat
Rat, embryonic
Maric, D. , Maric, I. and Barker, J.
Buoyant Density Gradient Fractionation and Flow Cytometric Analysis of Embryonic Rat Cortical Neurons and Progenitor Cells. ,
Methods
 16 , 247 , 1998
Cells
Neurons and progenitor
Enzymes
Papain : 20 u/ml
Medium
EBSS
Stem
Rat
Rat
Stemple, D. and Anderson, D.
Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest ,
Cell
 71 , 973 , 1992
Cells
Stem, neural crest
Enzymes
Collagenase : 0.075%
Medium
Ringer's solution
Thymus
Human
Human
Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K.
Human thymus contains 2 distinct dendritic cell populations ,
Blood
 97 , 1733-41 , 2001
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Thymus
Mouse
Mouse
Rode, I. and Boehm, T.
Regenerative Capacity of Adult Cortical Thymic Epithelial Cells. ,
Proc Natl Acad Sci U S A
 109 , 3463-8 , 2012
Cells
Thymic stromal
Enzymes
Medium
RPMI-1640
File
11532.pdf (1.16 MB)
Thymus
Mouse
Mouse
Phillips, Joy, Brondstetter, T. , English, C. , Lee, H. , Virts, E. and Thoman, M.
IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution ,
J Immunol
 , 4869 , 2004
Cells
Stromal
Enzymes
Medium
RPMI 1640
Thymus
Mouse
Mouse
Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L
ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells ,
J Exp Med
 196 , 1415 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Thymus
Mouse
Mouse, 3-6 week
Smith KM, Olson DC, Hirose R, Hanahan D.
Pancreatic gene expression in rare cells of thymic medulla: evidence for functional contribution to T cell tolerance ,
Int Immunol
 9 , 1355-65 , 1997
Cells
Thymic
Enzymes
Collagenase Type 3 : 100-400 u/ml
Medium
HBSS
Thymus
Mouse
Mouse (BALB/c, C3H, C57BL/6), 1-28 day
Ropke, C. , van Deurs, B. and Petersen, O.
Short-term Cultivation of Murine Thymic Epithelial Cells in a Serum-Free Medium ,
In Vitro Cell Dev Biol
 26 , 671 , 1990
Cells
Epithelial, thymus
Enzymes
Neutral Protease : 1.5 µg/ml
Medium
DMEM
Thymus
Mouse
Mouse, C3H, 16 wk old, female
Ehmann, U. , Shiurba, R. and Peterson, W.
Long-Term Proliferation of Mouse Thymic Epithelial Cells in Culture ,
In Vitro Cell Dev Biol
 22 (12) , 738 , 1986
Cells
Epithelial
Enzymes
Medium
DMEM
Thymus
Mouse
Mouse, Swiss, 6 wk
Jones, K. and Pierre, R.
Analysis of Cellular Heterogeneity in Mouse Thymus Cultures ,
In Vitro
 17 , 431 , 1981
Cells
Thymus
Enzymes
Collagenase Type 3 : 150 u/ml
Medium
DMEM
Thymus
Rat
Rat, postnatal
Bonfanti, P. , Claudinot, S. , Amici, A. , Farley, A. , Blackburn, C. and Barrandon, Y.
Microenvironmental Reprogramming of Thymic Epithelial Cells to Skin Multipotent Stem Cells. ,
Nature
 466 , 978 , 2010
Cells
Thymic
Enzymes
Trypsin : 0.05%
Medium
HBSS
Thymus
Rat
Rat
Masuda, A. and Matsuyama, M
Epithelial Cell Lines From Rat Thymoma and Rat Thymus , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 21C:4.1 , 1995
Cells
Thymic epithelial
Enzymes
Medium
DMEM
Thymus
Rat
Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
Rat, (ACI/MNs) male, 8 weeks
(also bovine, adult)
Masuda, A. , Ohtsuka, K. and Matsuyama, M.
Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus ,
In Vitro Cell Dev Biol
 26 , 713 , 1990
Cells
Epthelial
Enzymes
Medium
Eagle's MEM Serum-free
Thyroid/Parathyroid
Bovine
Bovine, adult
Nygren, P. , Gylfe, E. , Larsson, R. , Johansson, H. , Juhlin, C. , Klareskoq, L. , Akerstrom, G. and Rastad, J.
Modulation of the Ca2+-Sensing Function of Parathyroid Cells In Vitro and in Hyperparathyroidism ,
Biochim Biophys Acta
 968 , 253 , 1988
Cells
Parathyroid
Enzymes
Medium
HEPES Ham's F10
Thyroid/Parathyroid
Bovine
Bovine
Wallace, J. and Scarpa, A.
Regulation of Parathyroid Hormone Secretion in Vitro by Divalent Cations ,
J Biol Chem
 257 , 10613 , 1982
Cells
Parathyroid glands
Enzymes
Medium
HEPES buffer
Thyroid/Parathyroid
Bovine
Bovine
Brown, E. , Hurwitz, S. and Aurbach, G.
Preparation of Viable Isolated Bovine Parathyroid Cells ,
Endocrinology
 99 , 1582 , 1976
Cells
Parathyroid
Enzymes
Medium
Eagle's #2 medium without bicarbonate
Thyroid/Parathyroid
Bovine
Bovine
(also porcine)
Tong, W.
The Isolation and Culture of Thyroid Cells ,
Meth Enzymol
 32 , 745 , 1974
Cells
Thyroid
Enzymes
Trypsin : 0.004%
Medium
EBSS
Thyroid/Parathyroid
Chicken
Chick embryo
Hilfer, S. and Brown, J.
Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart ,
Exp Cell Res
 65 , 246 , 1971
Cells
Thyroid
Muscle
Heart
Enzymes
Collagenase : 0.25%
Medium
Tyrode's saline, potassium free
Thyroid/Parathyroid
Chicken
Chicken, Rhode Island Red, embryo
Spooner, B.
The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture ,
J Cell Physiol
 75 , 33 , 1970
Cells
Thyroid follicular
Enzymes
Collagenase : 0.2%
Medium
Tyrode's solution, CMF
Thyroid/Parathyroid
Human

Human

Yi, S. , Kim, H. , Koo, B. , Lee, S. , Choi, J. and Kang, Y.
Efficient Dissociation Protocol for Generation of Single Cell Suspension from Human Thyroid Tissue for Single Cell RNA Sequencing. , Endocrinol Metab (Seoul) Vol. 37 , 698-700 , 2022
Cells
Thyroid normal and tumor
Enzymes
Trypsin : 0.125%
Collagenase Type 4 : 300 u/ml
Medium
RPMI-1640
File
Yi.2022.pdf (1.89 MB)
Thyroid/Parathyroid
Human
Human
Patel, V. , Logan, A. , Watkinson, J. , Uz-Zaman, S. , Sheppard, M. , Ramsden, J. and Eggo, M.
Isolation and Characterization of Human Thyroid Endothelial Cells. ,
Am J Physiol Endocrinol Metab
 284 , E168 , 2003
Cells
Endothelial
Enzymes
Medium
DMEM
File
10586.pdf (413.23 KB)
Thyroid/Parathyroid
Human
Human
Gianoukakis, A. , Cao, H. , Jennings, T. and Smith, T.
Prostaglandin Endoperoxide H Synthase Expression in Human Thyroid Epithelial Cells. ,
Am J Physiol Cell Physiol
 280 , C701 , 2001
Cells
Thyrocytes
Enzymes
Collagenase Type 1 : 130 u/ml
Neutral Protease : 0.5 u/ml
Medium
HBSS
File
10594.pdf (342.02 KB)
Thyroid/Parathyroid
Human
Human
Howie, A. , Walker, S. , Akesson, B. , Arthur, J. and Beckett, G.
Thyroidal Extracellular Glutathione Peroxidase: A Potential Regulator of Thyroid-Hormone Synthesis. ,
Biochem J
 308 ( Pt 3) , 713 , 1995
Cells
Thyrocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.1%
Medium
EBSS
File
10593.pdf (1.03 MB)
Thyroid/Parathyroid
Human
Human
Miller, R. , Hiraoka, T. , Nakamura, N. , Tenou, H. , Kopecky, K. , Jones, M. and Gould, M.
In Vitro Culture of Human Thyroid Cells; Methods and Application to Radiation Biology. ,
J Radiat Res
 26 , 269 , 1985
Cells
Thyroid
Enzymes
Collagenase : 300 u/ml
Medium
Ham's F-12/MEM
Thyroid/Parathyroid
Mouse
Mouse
Kitajima, S. , Kohno, S. , Kondoh, A. , Sasaki, N. , Nishimoto, Y. , Li, F. , Mohammed, M. , Muranaka, H. , Nagatani, N. , Suzuki, M. , Kido, Y. and Takahashi, C.
Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts. ,
Stem Cells
 33 , 1657-69 , 2015
Cells
Thyroid tumor
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM
File
11653.pdf (5.57 MB)
Thyroid/Parathyroid
Mouse
Mouse, 6 week
Martin, A. , Coronel, E. , Sano, G. , Chen, S. , Vassileva, G. , Canasto-Chibuque, C. , Sedgwick, J. , Frenette, P. , Lipp, M. , Furtado, G. and Lira, S.
A Novel Model for Lymphocytic Infiltration of the Thyroid Gland Generated by Transgenic Expression of the CC Chemokine CCL21. ,
J Immunol
 173 , 4791 , 2004
Cells
Thyroid
Enzymes
Neutral Protease : 0.0012 u/ml
Collagenase Type 2 : 0.25 u/ml
Medium
RPMI 1640
Thyroid/Parathyroid
Ovine
Sheep
Kerkof, P.
Preparation of Primary Cultures of Ovine Thyroid Gland Cells ,
J Tiss Cul Meth
 7 , 23 , 1982
Cells
Thyroid
Enzymes
Collagenase : 0.2%
Medium
Puck's Saline F
Thyroid/Parathyroid
Porcine
Porcine
(also bovine)
Tong, W.
The Isolation and Culture of Thyroid Cells ,
Meth Enzymol
 32 , 745 , 1974
Cells
Thyroid
Enzymes
Trypsin : 0.004%
Medium
EBSS
Thyroid/Parathyroid
Rat
Rat, Lewis, male, 4 week
Arauchi, A. , Shimizu, T. , Yamato, M. , Obara, T. and Okano, T.
Tissue-Engineered Thyroid Cell Sheet Rescued Hypothyroidism in Rat Models After Receiving Total Thyroidectomy Comparing with Nontransplantation Models. ,
Tissue Eng Part A
 15 , 3943 , 2009
Cells
Thyroid
Enzymes
Medium
DMEM
Tonsil
Human
Human
Muto, M. , Manfroi, B. , Suzuki, H. , Joh, K. , Nagai, M. , Wakai, S. and Righini, C. et al.
Toll-Like Receptor 9 Stimulation Induces Aberrant Expression of a Proliferation-Inducing Ligand by Tonsillar Germinal Center B Cells in IgA Nephropathy. ,
J Am Soc Nephrol
 28 , 1227-1238 , 2017
Cells
Tonsillar
Enzymes
Medium
see reference
File
11687.pdf (3.06 MB)
Tonsil
Human
Human
Schmidt, S. , Schenkova, K. , Adam, T. , Erikson, E. , Lehmann-Koch, J. , Sertel, S. , Verhasselt, B. , Fackler, O. , Lasitschka, F. and Keppler, O.
SAMHD1's Protein Expression Profile in Humans. ,
J Leukoc Biol
 98 , 5-14 , 2015
Cells
Tonsillar
Enzymes
Medium
PBS
File
11695.pdf (2.11 MB)
Tonsil
Human
Human
Sato, D. , Suzuki, Y. , Kano, T. , Suzuki, H. , Matsuoka, J. , Yokoi, H. , Horikoshi, S. , Ikeda, K. and Tomino, Y.
Tonsillar TLR9 Expression and Efficacy of Tonsillectomy with Steroid Pulse Therapy in IgA Nephropathy Patients. ,
Nephrol Dial Transplant
 27 , 1090-7 , 2012
Cells
Tonsillar
Enzymes
Medium
Bambanker
File
11686.pdf (364.81 KB)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.