Phosphodiesterase II - Worthington Enzyme Manual

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Spleen exonuclease (Phosphodiesterase II) hydrolyses 3'-phosphomononucleotides from oligonucleotides containing a 5'-hydroxyl terminus. See review by Bernardi and Bernardi (1971).

The enzyme's specificity for the 5'-phosphate end of a polynucleotide chain after conversion to 5'-OH by use of E. coli alkaline phosphatase makes spleen exonuclease very important in the characterization of polynucleotide chain length, base composition and identity of terminal nucleotide. See Ho and Hilham (1973), Philippsen and Zachau (1971), Roychoudhury et al. (1971), and >Sneider (1971).

Characteristics of Exonuclease from Bovine Spleen:

Specificity

Spleen exonuclease splits off, sequentially, nucleoside 3'-phosphates, starting at the 5'-OH end, from ribo- or deoxyribo-oligonucleotides. RNA "core", alkali-treated DNA, poly(A), poly(U), and poly(I) are acted upon. Prior removal of the 5'-terminal phosphate group is essential.

Native DNA and poly(C) are quite resistant. Bis-(p-nitrophenyl) phosphate is a very poor substrate. (Bernardi and Bernardi 1968).

Characteristics of Phosphodiesterase II

Optimal pH

5.5 using succinate and phosphate buffer and an acid DNase digest; pH 6-7 with 0.1 M acetate buffer. (Bernardi and Bernardi 1971).

Phosphodiesterase II Products

Description

Phosphodiesterase II

Source:

Bovine Spleen

Prepared from the 1mM sodium pyrophosphate, pH 6.9, alumina gel eluate of Hilmoe: Biochem. Prep VIII (Meister, A., ed.), John Wiley & Sons, NY, NY, p. 105 (1961).

Store at -20°C.

…

Ice Pack required

Activity

≥1.2 u/mg dw

Certificate of Analysis

Code

SPH

Product details

LS003603

10 un

$118.00

LS003602

25 un

$265.00

LS003600

Bulk

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Phosphodiesterase II - Manual

Characteristics of Exonuclease from Bovine Spleen:

Phosphodiesterase II Products