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Ribonuclease U2, Recombinant
Source:
Komagataella phaffii (previously P. pastoris)
CAS:
37205-57-5
EC:
4.6.1.20
Ustilago sphaerogena ribonuclease U2 produced recombinantly in yeast (Komagataella phaffi-previously P. pastoris) to decrease contaminant nucleases and proteases and eliminate potential pathogens associated with animal-sourced materials.
Ribonuclease U2 is an endonuclease that hydrolyzes the phosphodiester bonds of 3’-linked ribonucleotides, resulting in nucleoside 3’-phosphates and 3’-phosphooligonucleotides ending in Ap or Gp with 2’, 3’-cyclic intermediates. It is purine selective with a preference for adenosine and has the following order of cleavage specificity (A>G>C>U). Because of this, it is helpful in RNA sequencing and RNA modification and mapping applications. Optimal activity is observed at pH 4.5.
Stability/Storage : Stable for one year at 2-8oC. Store at 2-8oC
Unit Definition
One unit will cause an increase in absorbance of 1.0 at 260nm when yeast RNA is hydrolyzed at 37oC, pH 4.5 in 15 minutes.
Ribonuclease U2, Recombinant Products
Description
Activity
Code
Cat. #
Size
Price
Description
Ribonuclease U2, Recombinant, AF
Source:
Komagataella phaffii (previously P. pastoris)
Recombinant Ribonuclease U2 produced in Komagataella phaffii. Chromatographically purified. Supplied as a lyophilized powder, Animal Free/AF.
Store at 2-8°C.
Code
RU2R
Product details
LS01520
100 ugP
$185.00
LS01521
Bulk
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