Phosphodiesterase II - Assay

Prepare a 37°C water bath.

Pipette into tubes as follows:

	Test	Blank
Sodium succinate buffer	0.4 ml	0.4 ml
RNA core	0.5 ml	0.5 ml
Reagent grade water	1.0 ml	1.1 ml
Incubate tubes in 37°C water bath for 5 minutes to achieve temperature equilibration. At timed intervals add:
Enzyme dilution	0.1 ml	------

Incubate at 37°C for 30 minutes. Stop the reaction at timed intervals by adding 2.0 ml uranyl acetate/perchloric acid solution. Remove to an ice bath and chill for 10 minutes. Centrifuge at high speed in a clinical centrifuge for 15 minutes. Transfer 0.2 ml aliquot of each supernatant to a clean dry test tube and add 7.8 ml reagent grade water. Mix well and read A₂₆₀ vs water.