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Phosphodiesterase II

Source:
Bovine Spleen
CAS:
9068-54-6
EC:
3.1.16.1
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I.U.B.: 3.1.16.1

Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique.

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Unit Definition

One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37°C, pH 6.5, with an RNA substrate.

Phosphodiesterase II Products

Description
Activity
Code
Cat. #
Size
Price
Description
Phosphodiesterase II
Source:
Bovine Spleen
Prepared from the 1mM sodium pyrophosphate, pH 6.9, alumina gel eluate of Hilmoe: Biochem. Prep VIII (Meister, A., ed.), John Wiley & Sons, NY, NY, p. 105 (1961).
Store at -20°C.
…
Ice Pack required
Activity
≥1.2 u/mg dw
Code
SPH
LS003603
10 un
$118.00
LS003602
25 un
$265.00
LS003600
Bulk
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The minimum amount for bulk packaging/pricing for this product is 500 x 1 un. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
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Albumin, Nuclease-Free
Deoxyribonuclease I
Histones
Lysozyme
Nuclease, Micrococcal
Nuclease, S1
Deoxyribonucleic Acid and Related Products
Ribonucleic Acid
Phosphatase, Alkaline
Phosphodiesterase I
Proteinase K
Reverse Transcriptase, Recombinant HIV
Ribonuclease A
Ribonuclease T1