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Nuclease, Micrococcal
Source:
Staphylococcus aureus (Strain ATCC #27735)
CAS:
9013-53-0
EC:
3.1.31.1
I.U.B.: 3.1.31.1
Micrococcal nuclease catalyzes cleavage of both DNA and RNA to yield 3'-nucleotides. It exhibits exo- and endo-5'-phosphodiesterase activities. The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate. The enzyme has a molecular weight of 16,807 daltons and is calcium dependent. The pH optimum is 9.2 but varies depending upon the concentration of ionized calcium present.
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Unit Definition
One Unit corresponds to a change in optical density of 1.0 at 260 nm at 37°C, pH 8.0, using DNA as the substrate.
Nuclease, Micrococcal Products
Description
Activity
Code
Cat. #
Size
Price
Description
Nuclease, Micrococcal
Source:
Staphylococcus aureus (Strain ATCC #27735)
Chromatographically purified to be essentially homogeneous chromatographically and electrophoretically (SDS-PAGE). A lyophilized powder.
Store at 2-8°C.
Code
NFCP
Product details
LS004797
15 ku
$98.00
LS004798
45 ku
$220.00
LS004796
Bulk
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