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Proteinase K References

Source:
Recombinant PROK produced in yeast
CAS:
39450-01-6
EC:
3.4.21.64
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Bernabeu, C. , Conde, P. , Vasquez, D. and Ballesta, J.
Peptidyl Transferase of Bacterial Ribosome: Resistance to Proteinase K ,
Eur J Biochem
 93 , 527 , 1979
Betzel, C. , Pal, G. , Struck, M. , Jany, K. and Saenger, W.
Active-Site Geometry of Proteinase K: Crystallographic Study of Its Complex with a Dipeptide Chloromethyl Ketone Inhibitor ,
F.E.B.S. Lett.
 197 , 105 , 1986
Brdicka, D. and Krebs, W.
Localization of Enzymes by Means of Proteases ,
Biochim Biophys Acta
 297 , 203 , 1972
Broemme, D. , Peters, K. , Fink, S. and Fittkau, S.
Enzyme-Substrate Interactions in the Hydrolysis of Peptide Substrates by Thermitase, Subtilisin BPN', and Proteinase K ,
Arch Biochem Biophys
 244 , 439 , 1986
Crowe, J. , Cooper, H. , Smith, M. , Sims, M. , Parker, D. and Gewert, D.
Improved Cloning Efficiency of Polymerase Chain Reaction (PCR) Products After Proteinase-K Digestion ,
Nucleic Acids Res
 19 , 184 , 1991
Cryer, D. , Eccleshall, R. and Marmur, J.
Isolation of Yeast DNA ,
Methods Cell Biol
 12 , 39 , 1975
Cryer, D. , Eccleshall, R. and Marmur, J.
Isolation of Yeast DNA , Cell Biology Vol. 12 , 39 , 1975
Dattagupta, J. , Fujiwara, T. , Grishin, E. , Lindner, K. , Manor, P. , Pieniazek, N. , Saenger, W. and Suck, D.
Crystallization of the Fungal Enzyme Proteinase K and Amino Acid Composition ,
J Mol Biol
 97 , 267 , 1975
Ebeling, W. , Hennrich, N. , Klockow, M. , Metz, H. , Orth, H. and Lang, F.
Proteinase K from Tritirachium album Limber ,
Eur J Biochem
 47 , 91 , 1974
Fourcroy, P.
Isolation of Undegraded Polysomes from Radish Cotyledons: Use of Proteinase K and Cycloheximide ,
Phytochem
 19 , 7 , 1980
Gajda, A. , Rudenskaya, G. and Stepanov, V.
Isolation and Comparative Properties of Serine Proteinases of the Microscopic Fungi Trichoderma lignorum and Trichoderma koningii ,
Biokhimiia
 46 , 2064 , 1981
Hansen, J.
Isolation of Higher Molecular Weight DNA from Bacillus cereus T Using Proteinase K ,
Prep Biochem
 4 , 473 , 1974
Hilz, H. , Wiegers, U. and Adamietz, P.
Stimulation of Proteinase K Action by Denaturing Agents; Application to the Isolation of Nucleic Acids and the Degradation of "Masked Proteins" ,
Eur J Biochem
 56 , 103 , 1975
Holm, C. , Meeks-Wagner, D. , Fangman, W. and Botstein, D.
A Rapid, Efficient Method for Isolating DNA from Yeast ,
Gene
 42 , 169 , 1986
Jany, K. , Lederer, G. and Mayer, B.
Amino Acid Sequence of Proteinase K from the Mold Tritirachiium Album limber. Proteinase K: A Subtilisin-related Enzyme with Disulfide Bonds ,
F.E.B.S. Lett.
 199 , 139 , 1986
Jany, K. and Mayer, B.
Proteinase K from Tritirachium Album limber: Molecular Mass and Sequence Around the Active Site Serine Residue ,
Biol Chem Hoppe Seyler
 366 , 485 , 1985
Jehanli, A. and Hough, D.
Pronase and Proteinase K Digestion of Human Immunoglobulin M ,
Mol Immunol
 22 , 557 , 1985
Kasche, V. , Zollner, R. , Amneus, H. and Naslund, L.
A Two-Step Procedure for Quantitative Isolation of Pure Double Strand DNA From Animal Tissus and Cell Cultures ,
Prep Biochem
 11 , 233 , 1981
Kraus, E. and Femfert, U.
Proteinase K from the Mold Tritarachium album Limber. Specificity and Mode of Action ,
Hoppe-Seylers Z Physiol Chem
 357 , 937 , 1976
Lizardi, P. and Engelberg, A.
Rapid Isolation of RNA Using Proteinase K and Sodium Perchlorate ,
Anal Biochem
 98 , 116 , 1979
Lough, J. , Wrenn, D. , Miziorko, H. and Auer, H.
Differential Sensitivity of Chicken MM-Creatine Kinase to Trypsin and Proteinase K ,
Int J Biochem
 17 , 309 , 1985
McCormick, J. , Larson, L. and Maher, V.
Problems in the Extraction of DNA when Utilizing Pancreatic RNAase and Pronase ,
Biochim Biophys Acta
 349 , 145 , 1974
McGodkin, R.
RNA Extraction by the Proteinase K Method ,
Methods Mol Biol
 2 , 109 , 1984
Mendolsohn, S. and Young, D.
Inhibition of Ribonuclease: Efficacy of Sodium Dodecyl Sulfate, Diethyl Pyrocarbonate, Proteinase K and Heparin Using a Sensitive Ribonuclease Assay ,
Biochim Biophys Acta
 519 , 461 , 1978
Morihara, K. and Tsuzuki, H.
Specificity of Proteinase K from Tritirachium album Limber for Synthetic Peptides ,
Agric Biol Chem
 39 , 1489 , 1975
Muller, A. , Hinrichs, W. , Wolf, W. and Saenger, W.
Crystal Structure of Calcium-free Proteinase K at 1.5-A Resolution ,
J Biol Chem
 37 , 23108 , 1994
Orstan, A. and Gafni, A.
Inhibition of Proteinase-K by Phosphorylated Sugars ,
Biochem Int
 25 , 657 , 1991
Paehler, A. , Banerjee, A. , Dattagupta, J. , Fujiwara, T. , Lindner, K. , Pal, G. , Suck, D. , Weber, G. and Saenger, W.
Three-Dimensional Structure of Fungal Proteinase K Reveals Similarity to Bacterial Subtilisin ,
EMBO J
 3 , 1311 , 1984
Pal, G. , Betzel, C. , Jany, K. and Saenger, W.
Crystallization of the Bifunctional Proteinase/Amylase Inhibitor PKI-3 and of Its Complex with Proteinase K ,
F.E.B.S. Lett.
 197 , 111 , 1986
Petsch, D. , Deckwer, W. and Anspach, F.,
Proteinase K Digestion of Proteins Improves Detection of Bacterial Endotoxins by the Limulus Amebocyte Lysate Assay: Application for Endotoxin Removal from Cationic Proteins ,
Anal Biochem
 259 , 42 , 1998
Rauber, N. , Jany, K. and Pfleiderer, G.
RNase A Digestion by Proteinase K , Z ,
Naturforsch
 33, Sect.C , 9 , 1978
Roelcke, D. and Uhlenbruck, G.
Proteinase K: A New Serological Effective Protease from Fungi , Z. Med. Mikrobiol. Imm. Vol. 155 , 156 , 1969
Veerisetty, V. and Sehgal, O.
Proteinase K - Sensitive Factor Essential for the Infectivity of Southern Bean Mosaic Virus Ribonucleic Acid ,
Phytopathology
 70 , 282 , 1980
Vidales, F. , Bernabeu, C. and Ballesta, J.
Peptidyl Transfer Ribonucleic Acid Hydrolase Activity of Proteinase K ,
Biochemistry
 18 , 4155 , 1979
Wiegers, U. and Hilz, H.
A New Method Using Proteinase K to Prevent mRNA Degradation During Isolation from HeLa Cells ,
Biochem Biophys Res Commun
 44 , 513 , 1971
Wiegers, U. and Hilz, H.
Rapid Isolation of Undegraded Polysomal RNA without Phenol ,
F.E.B.S. Lett.
 23 , 77 , 1972

Proteinase K Products

Description
Activity
Code
Cat. #
Size
Price
Description
Proteinase K, Solution
Source:
Tritirachium album limber
A concentrated, ready to use liquid formulation. Proteinase K prepared at 20mg/ml in 10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. DNase- and RNase- free.
Store at -20°C.
…
Ice Pack required
Activity
≥400 u/ml
Code
PROKS
Description
Proteinase K Recombinant, Animal Free/AF
Source:
Recombinant PROK produced in yeast
Animal Free/AF, Molecular Biology Grade purified recombinant Tritiachium album Proteinase K produced in yeast. Free of DNase and RNase. Supplied as a lyophilized powder. Store at 2-8°C.
Store at 2-8°C.
…
Activity
>20 units per mg dry weight
Code
PROKR
LS004248
25 mg
$44.00
LS004249
100 mg
$93.00
LS004250
1 gm
$710.00
LS004252
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Proteinase K, Recombinant Solution
Source:
Tritirachium album limber
A concentrated, ready to use liquid formulation. Recombinant Proteinase K prepared at 20mg/ml in 10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. DNase and RNase free. Store at -20°C.
Store at -20°C.
…
Ice Pack required
Activity
>400 u/ml
Code
PROKRS
LS004254
5 ml
$140.00
LS004256
25 ml
$570.00
LS004258
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 500 x 1 ml. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
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