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Nuclease, S1 - Manual
Source:
Aspergillus oryzae
CAS:
37288-25-8
EC:
3.1.30.1
Nuclease S1, isolated from certain Neurospora and Aspergillus species, specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. It is used to eliminate non-annealed polynucleotide tails and hair-pin loops in DNA-RNA or DNA-DNA duplexes in hybridization studies and in genetic recombination experiments.
Characteristics of Nuclease, S1
Molecular Weight
Approximately 32,000 - 36,000 daltons, exists as a monomer. (Vogt, V. 1973).
Optimal pH
4.0 - 4.6. (Vogt 1973 and Ando 1966).
Activators
Zn++ and/or Ca++. (Vogt 1973 and Ando 1966).
Inhibitors
EDTA, citrate (Vogt 1973 and Ando 1966) and a high concentration of SDS.
Storage
Store at -20°C.
Nuclease, S1 Products
Description
Activity
Code
Cat. #
Size
Price
Description
Nuclease, S1
Source:
Aspergillus oryzae
Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.
Store at -20°C.
Ice Pack required
Code
SINUC
Product details
LS04070
10 ku
$60.00
LS04072
50 ku
$162.00
LS04073
Bulk
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