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Enzyme Manual

The Worthington Enzyme Manual contains technical information on enzymes including molecular weight, composition, activators, specificity, inhibitors, stability, assay method, optimum pH, ionic effects, temperature effects, extinction coefficient, and isoelectric point, as well as applications and extensive references.

Worthington Enzyme Manual
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Inhibitors sourced from soybean, lima bean or ovomucoid which are tested for their ability to inhibit trypsin. Soybean trypsin inhibitor (SBTI) first crystallized by Kunitz (1945) is one of several…
Trypsinogen is the 24kDa inactive precursor of trypsin which nay be activated by enzymatic removal of an n-terminal hexapeptide to yield single chain beta-trypsin. Subsequent limited autolysis…
Tyrosine decarboxylase catalyzes the removal of the carboxyl group from tyrosine to produce tyramine and carbon dioxide: Pyridoxal 5'-phosphate is a necessary coenzyme. By utilizing the apoenzyme…
Urease catalyzes the hydrolysis of urea: Jespersen (1975) reports that ammonium carbamate is produced in citrate and Tris buffer. Urease occurs in many bacteria, several species of yeast and a…
Uricase (urate oxidase) catalyzes the following overall reaction: This reaction represents the termination of purine catabolism in all mammals excluding man, higher apes and the Dalmatian dog.…
Xanthine oxidase catalyzes the oxidation of xanthine to uric acid: Characteristics of Xanthine Oxidase from Milk: Xanthine oxidase, being a unimolecular, multicomponent electron transport systm…