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Neuraminidase - Manual

Source:
Clostridium perfringens
CAS:
9001-67-6
EC:
3.2.1.18
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Neuraminidase (sialidase) splits off N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins.

formula

Neuraminidases are important tools for the study of glycoproteins in protein chemistry and cell biology (Hatton et al. 1973).

Worthington neuraminidase is derived from Clostridium perfringens. Chromatographic purification has been developed in this laboratory based on work of Cassidy et al. (1965). Wooley and Gommi (1966) describe the use of this neuraminidase in a method for measuring serotonin receptors. It is also employed to determine bound N-acetylneuraminic acid in tissues.

Characteristics of Neuraminidase from Clostridium perfringens:

Characteristics of Neuraminidase
Optimal pH

5.0-5.1 (Burton 1963). Little or no activity at pH 4.0 or above pH 8.0.

Isoelectric point

5.1 (Groome and Belyaven 1958).

Activators

None, in contrast to the neuraminidase from Vibrio which has a divalent metal requirement.

Inhibitors

Considerable interest has been shown in using neuraminidase inhibitors as possible anti-viral and anti-bacterial agents. (Khorlin et al. 1970; Haskell et al. 1970; and Tute 1970).

Stability

The purified preparation is stable for at least two years when stored at 4°C (Cassidy et al. 1965).

Stabilizers

Serum albumin, 0.3 mg/ml (Cassidy et al. 1965).

Neuraminidase Products

Description
Activity
Code
Cat. #
Size
Price
Description
Neuraminidase, Purified
Source:
Clostridium perfringens
Chromatographically purified. A lyophilized powder containing 50% (w/w) sucrose. Contaminating proteolytic activity 0.1% using trypsin as the standard.
Store at 2-8°C. PROTECT FROM MOISTURE.
…
Activity
≥10 units per mg protein
Code
NEUA
LS004759
5 un
$110.00
LS004761
10 un
$212.00
LS004762
25 un
$440.00
LS004760
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 500 x 1 un. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Neuraminidase
Source:
Clostridium perfringens
A partially purified, lyophilized powder.
Store at 2-8°C.
…
Activity
≥0.5 units per mg dry weight
Code
NEUP
LS004779
4 mg
$117.00
LS004780
10 mg
$238.00
LS004777
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 200 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
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