Hydroxysteroid Dehydrogenase - Manual

Source:
Pseudomonas testosteroni
CAS:
9028-56-2
9015-81-0
EC:
1.1.1.51

Hydroxysteroid dehydrogenases catalyze the interconversion of hydroxyl and carboxyl groups of steroids. Those from Pseudomonas testosteroni are of two types:

1. 3α-Hydroxysteroid Dehydrogenase (1.1.1.50), (α enzyme):

formula

2. 3β- and 17β-Hydroxysteroid Dehydrogenase:

formula

The enzymes have useful application in the assay of steroids (Hurlock and Talalay 1958; Stempfel and Sidbury 1964; Moore 1972). They have been used for the determination of bile acids in blood (Iwata and Yamasaki 1964; Palmer 1969; Leslie 1969; Murphy et al. 1970; Schwarz et al. 1974).

It should be noted that Worthington supplies two preparations: one from the regular Pseudomonas testosteroni (ATCC 11966) culture which produces both the α and β enzymes, and a second from a mutant strain which produces almost only the α enzyme. By using both, the β-hydroxysteroid can be determined by difference.

Teller and Bongiavanni (1963) reported only slight activity of the mutant strain on epiandrosterone indicating but a trace of the β enzyme. Roe and Kaplan (1969) have investigated the specificities of the mutant and wild type strains, and this reference should be consulted for specific details.

Characteristics of Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni:

3α hydroxysteroid dehydrogenase

Specificity

α enzyme oxidizes only 3α-hydroxysteroids of the C19, C21, C24 series (Talalay 1963).

Composition

Amino acid composition has been reported by Squire et al. (1964).

Characteristics of Hydroxysteroid Dehydrogenase
Molecular Weight

47,000 (Squire et al. 1964; Skålhegg 1974).

Isoelectric point

pH 6.1 (Squire et al. 1964).

Inhibitors

Heavy metals and sulfhydryl-binding reducing agents (Talalay 1963).

Stability

The Worthington preparation of a enzyme is stable for 6-12 months when stored at -20°C.

3β (or 17β)-hydroxysteriod dehydrogenase

100,000 (Squire et al. 1964).

pH 6.5 (Squire et al. 1964).

Catalyzes the oxidation of 3β-hydroxysteroids of the C19 and C21 series, 17β-hydroxysteroids of the C18, C19, and C21 series, as well as certain 16β-hydroxysteroids (Talalay 1963).

Heavy metals and reducing agents. The oxidation of testosterone is inhibited by 3,17α-estradiol and other 1,3,5-estratriene derivatives (Talalay 1963).

The Worthington preparation of mixed α and β enzyme is stable for 6-12 months when stored at -20°C.

Hydroxysteroid Dehydrogenase Products

Description
Activity
Code
Cat. #
Size
Price
Description
Hydroxysteroid Dehydrogenase
Source:
Pseudomonas testosteroni
A lyophilized powder obtained from induced cells. Contains both alpha and beta activities.
Store at -20°C.
Ice Pack required
Activity
≥0.03 units per mg dry weight
Code
STDH
LS004915
1 gm
$87.00
LS004916
5 gm
$349.00
LS004918
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 100 x 1 gm. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Hydroxysteroid Dehydrogenase
Source:
mutant: Pseudomonas testosteroni
A purified powder obtained from adapted cells of a mutant strain. Activity on androsterone only, no activity exhibited on testosterone.
Store at -20°C.
Ice Pack required
Activity
≥0.5 units per mg dry weight
Code
STDHMP
LS004908
10 un
$108.00
LS004910
50 un
$385.00
LS004911
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 1000 x 1 un. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Hydroxysteroid Dehydrogenase
Source:
Pseudomonas testosteroni
A purified powder obtained from induced cells. Contains both alpha and beta activities.
Store at -20°C.
Ice Pack required
Activity
≥0.5 units per mg dry weight
Code
STDHP
Minimum order quantity
5000
LS004922
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 5000 x 1 un. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.