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Amino Acid Oxidase, L- - Assay
Source:
Crotalus adamanteus Venom
CAS:
9000-89-9
EC:
1.4.3.2
Amino Acid Oxidase, L- Assay
Method
The reaction velocity is determined in a peroxidase coupled system by measuring the increase in A436 resulting from the oxidation of L-leucine. One unit oxidizes one micromole of L-leucine per minute at 25°C and pH 7.6 under the specified conditions.
Reagents
- 0.2 M Triethanolamine buffer pH 7.6 containing 0.1% L-leucine and 0.0065% o-dianisidine
- 1.0% Peroxidase: Dissolve Worthington Peroxidase (HPOD) at 10 mg/ml in water.
Enzyme
Dilute enzyme in reagent grade water to 0.05-0.2 units per milliliter.
Procedure
Adjust spectrophotometer to 436 nm and 25°C.
Pipette into cuvettes 0.01 ml of 10 mg/ml peroxidase and 2.9 ml of 0.2 M triethanolamine-leucine-o-dianisidine mixture.
Incubate in spectrophotometer at 25°C for 4-5 minutes to achieve temperature equilibration and record blank rate, if any. Add 0.1 ml of appropriately diluted enzyme and record increase in absorbance at 436 nm for 4-5 minutes. Calculate ΔA436 from the initial linear portion of the slope. Subtract blank rate if present.
Calculation
Amino Acid Oxidase, L- Products
Description
Activity
Code
Cat. #
Size
Price
Description
Amino Acid Oxidase, L-
Source:
Crotalus adamanteus Venom
An aqueous solution with toluene added as a preservative.
Store at 2-8°C. DO NOT FREEZE.
Code
LAO
Product details
LS002763
2 mg
$92.00
LS002764
5 mg
$186.00
LS002766
Bulk
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