Papain References

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Akahane, K. and Umeyama, H.

Binding Specificity of Papain and Cathepsin B ,
Enzyme
36 , 141 , 1986

Alecio, M. , Dann, M. and Lowe, G.

The Specificity of the S1 Subsite of Papain ,
Biochem J
141 , 495 , 1974

Allen, G. and Lowe, G.

Investigation of the Active Site of Papain with Fluorescent Probes ,
Biochem J
133 , 679 , 1973

Allison, W. and Swain, L.

The Inactivation of Papain and Glyceraldehyde-3-Phosphate Dehydrogenase by Phenyldiimide ,
Arch Biochem Biophys
155 , 405 , 1973

Angus, R. , Carey, P. , Lee, H. and Storer, A.

Conformational Activation of Acylpapain and Acylcathepsin B's: Resonance Raman and Kinetic Evidence ,
Biochemistry
25 , 3304 , 1986

Arnon, R. and Shapira, E.

Antibodies to Papain. A Selective Fractionation According to Inhibitory Capacity ,
Biochemistry
6 , 3942 , 1967

Arnon, R.

Papain , Methods in Enzymology Vol. 19 , G. Perlmann and L. Lorand , Academic Press, NY , 226 , 1970

Asboth, B. , Stokum E. , Khan, I. and Polgar, L.

Mechanism of Action of Cysteine Proteinases. Oxyanion Binding Site Is Not Essential in the Hydrolysis of Specific Substrates ,
Biochemistry
24 , 606 , 1985

Azarkan, M. , El Moussaoui A. , van Wuytswinkel, D. , Dehon, G. and Looze, Y.

Fractionation and Purification of the Enzymes Stored in the Latex of Carica papaya ,
J Chromatogr B Analyt Technol Biomed Life Sci
790 , 229 , 2003

Balls, A. and Lineweaver, H.

Isolation and Properties of Crystalline Papain ,
J Biol Chem
130 , 669 , 1939

Banks, T. and Shafer, J.

Inactivation of Papain by S-Methylation of Its Cysteinyl Residue with O-Methylisourea ,
Biochemistry
11 , 110 , 1972

Barrett, A. and Buttle, D.

Names and Numbers of Papaya Proteinases ,
Biochem J
228 , 527 , 1985

Blumberg, S. , Schechter, I. and Berger, A.

The Purification of Papain by Affinity Chromatography ,
Eur J Biochem
15 , 97 , 1970

Brisson, J. , Carey, P. and Storer, A.

Benzoylamidoacetonitrile Is Bound as a Thiomidate in the Active Site of Papain ,
J Biol Chem
261 , 9087 , 1986

Brocklehurst, K. and Kierstan, M.

Propapain and Its Conversion to Papain: A New Type of Zymogen Activation Mechanism Involving Intramolecular Thiol-Disulfide Interchange ,
Nature New Biol
242 , 167 , 1973

Brocklehurst, K. and Little, G.

Reactions of Papain and of Low-Molecular-Weight Thiols with Some Aromatic Disulfides ,
Biochem J
133 , 67 , 1973

Brocklehurst, K. and Salih, E.

A Re-evaluation of the Nomenclature of the Cysteine Proteinases of Carica Papaya and a Rational Basis for Their Identification ,
Biochem J
213 , 559 , 1983

Brocklehurst, K. , Carey, P. , Lee, H. , Sahih, E. and Storer, A.

Comparative Resonance Raman Spectroscopic and Kinetic Studies of Acyl-enzymes Involving Papain, Actinidin and Papaya Peptidase II ,
Biochem J
223 , 649 , 1984

Brocklehurst, K. , Kierstan, M. and Little, G.

The Reaction of Papain with Ellman's Reagent (5,5'-Dithiobis-2-nitrobenzoate Dianion) ,
Biochem J
128 , 811 , 1972

Brubacher, L. and Bender, M.

The Preparation and Properties of Trans-Cinnamoyl-Papain ,
J Am Chem Soc
88 , 5871 , 1966

Brubacher, L. and Bender, M.

The Reaction of Trans-Cinnamoyl-Papain with a Series of Polyglycinamides of Varying Chain Length ,
Biochem Biophys Res Commun
27 , 176 , 1967

Bryant, J. , Leder, I. and Stetten, D.

The Release of Chondroitin Sulfate from Rabbit Cartilage Following the Intravenous Injection of Crude Papain ,
Arch Biochem Biophys
76 , 122 , 1958

Campbell, P. and Kaiser, E.

The Reaction of A Cyclic Sulfonate Ester with the Sulfhydryl Proteolytic Enzyme Papain ,
Bioorg Chem
1 , 432 , 1971

Campbell, P. and Kaiser, E.

The Intramolecular Nucleophilic Reaction of a Phenolic Hydroxyl Group at the Active Site of Papain ,
Biochem Biophys Res Commun
48 , 866 , 1972

Carotti, A. , Smith, R. , Wong, S. , Hansch, C. , Blaney, J. and Langridge, R.

Papain Hydrolysis of X-Phenyl-N-methane-sulfonyl Glycinates: A Quantitative Structure-Activity Relationship and Molecular Graphics Analysis ,
Arch Biochem Biophys
229 , 112 , 1984

Chaiken, I. and Smith, E.

Reaction of a Specific Tyrosine Residue of Papain with Diisopropylfluorophosphate ,
J Biol Chem
244 , 4247 , 1969

Choudhury, D. , Roy, S. , Chakrabarti, C. , Biswas, S. and Dattagupta, J.

Production and Recovery of Recombinant Propapain with High Yield ,
Phytochem
70 , 465-72 , 2009

Clagett, J. and Englehard, W.

Immunosuppressive Activity of the 3-alpha-1 Fraction of Papain ,
J Appl Bacteriol
93 , 1493 , 1967

Coates, J. and Swann, J.

A Reinvestigation of the Association Behaviour of Papain in Solution ,
Biochim Biophys Acta
214 , 545 , 1970

Cohen, L. , Fluharty, C. and Dihel, L.

Synthesis of Papain in Escherichia coli ,
Gene
88 , 263 , 1990

Cohen, L. , Coghlan, V. and Dihel, L.

Cloning and Sequencing of Papain-Encoding cDNA ,
Gene
48 , 219 , 1986

Cohen, W. and Petra, P.

A Kinetic Analysis of the Papain-Catalyzed Hydrolysis of alpha-N-Benzoyl-L-citrulline Methyl Ester ,
Biochemistry
6 , 1047 , 1967

deJersey, J.

On the Specificity of Papain ,
Biochemistry
9 , 1761 , 1970

Dekeyser, P. , Smedt, S. , Demeester, J. and Lauwers, A.

Fractioning and Purification of the Thiol Protinases from Papaya Latex ,
J Chromatogr B Biomed Appl
656 , 203 , 1994

Drenth, J. , Jansonius, J. , Koekoek, R. , Swen, H. and Wolters, B.

Structure of Papain ,
Nature
218 , 929 , 1968

Fink, A. and Gwyn, C.

Conformational Changes in Papain During Catalysis and Ligand Binding ,
Biochemistry
13 , 1190 , 1974

Finkbeiner, S. and Stevens, C.F.

Applications of Quantitative Measurements for Assessing Glutamate Neurotoxicity ,
Proc Natl Acad Sci U S A
85 , 4071 , 1988

Frater, R. , Light, A. and Smith, E.

Chemical and Enzymic Studies on the Amino-Terminal Sequence of Papain. A Reinvestigation ,
J Biol Chem
241 , 253 , 1966

Fujimoto, S. , Kanazawa, H. , Ishimitsu, S. and Ohara, A.

On the Mechanism of Inactivation of Papain by Hydroxylamine ,
Chem Pharm Bull (Tokyo)
38 , 546 , 1990

Glazer, A.

Transesterification Reactions Catalyzed by Papain ,
J Biol Chem
241 , 3811 , 1966

Glick, B. and Brubacher, L.

Evidence for Nonproductive Binding Subsites Within the Active Site of Papain ,
Can J Biochem
52 , 877 , 1974

Hall, P. , Anderson, C. and Crawford, G.

Acridine Dyes as Effectors in Papain Catalysis ,
Arch Biochem Biophys
153 , 162 , 1972

Henry, A. and Kirsch, J.

Papain-Catalyzed Reactions of Esters with Alcohols. The Nature of the Rate-Determining Step ,
Biochemistry
6 , 3536 , 1967

Hill, R. and Smith, E.

,
Biochim Biophys Acta
19 , 376 , 1956

Hinkle, P. and Kirsch, J.

Evidence for Conformational Restrictions within the Active Site of Acyl Papains Which Influence the Rates of Hydrolysis ,
Biochemistry
9 , 4633 , 1970

Hinkle, P. and Kirsch, J.

Evidence for Conformational Restrictions within the Active Site of Acyl Papains Which Influence the Rates of Hydrolysis. Correction ,
Biochemistry
10 , 1510 , 1971

Hinkle, P. and Kirsch, J.

Kinetics and Thermodynamics of the Reactions of Acyl-Papains. Effect of pH, Temperature, Solvents, Ionic Strength and Added Nucleophiles ,
Biochemistry
10 , 3700 , 1971

Hu, L. and Abeles, R.

Inhibition of Cathepsin B and Papain by Peptidyl alpha-Keto Esters, alpha-Keto Amides, alpha-Diketones, and alpha-Keto Acids ,
Arch Biochem Biophys
281 , 271 , 1990

Iso, N. and Williams, J.

Sedimentation Analysis of a Papain-Digested Human Multiple Myeloma gamma-G-Globulin ,
J Biol Chem
241 , 2787 , 1966

Jori, G. , Gennari, G. , Toniolo, C. and Scoffone, E.

Probing the Topography of Proteins in Solution by Photosensitized Oxidation, The Catalytic Region of Papain ,
J Mol Biol
59 , 151 , 1971

Kamphuis, I. , Kalk, K. , Swarte, M. and Drenth, J.

Structure of Papain Refined at 1.65 A Resolution ,
J Mol Biol
179 , 233 , 1984

Kanazawa, H. , Fujimoto, S. and Ohara, A.

On the Mechanism of Inactivation of Active Papain by Ascorbic Acid in the Presence of Cupric Ions ,
Biol Pharm Bull
17 , 189 , 1994

Kimmel, J. and Smith, E.

Crystalline Papain. I. Preparation, Specificity and Activation ,
J Biol Chem
207 , 515 , 1954

Kimmel, J. , Rogers, H. and Smith, E.

Tryptic Digest of Papain. II. Isolation and Sequences of Peptides from the Carboxymethylated Protein ,
J Biol Chem
240 , 266 , 1965

Kirschenbaum, D.

The Enhancement of the Enzymatic Activity of Papain by Reaction with N-Bromosuccinimide ,
Biochim Biophys Acta
235 , 159 , 1971

Klein, I. and Kirsch, J.

The Activation of Papain and the Inhibition of the Active Enzyme by Carbonyl Reagents ,
J Biol Chem
244 , 5928 , 1969

Kouvonen, I.

Solubilization of the Pig Ileal Intrinsic Factor Receptor with Papain Treatment and Studies on the Solubilized Receptor ,
Biochim Biophys Acta
626 , 244 , 1980

Lake, A. and Lowe, G.

The Kinetics of Papain- and Ficin-Catalyzed Hydrolyses in the Presence of Alcohols ,
Biochem J
101 , 402 , 1966

Lewis, S. and Shafer, J.

Ionization of a Nitrophenol-Containing Reporter Group at the Active Site of Papain ,
Biochemistry
13 , 690 , 1974

Liener, I.

The Sulfhydryl Proteases , Food Related Enzymes, Adv. in Chem. Series 136 , J. Whitaker , Amer. Chem. Soc., Washington, DC , 202 , 1974

Light, A. , Frater, R. , Kimmel, J. and Smith, E.

Current Status of the Structure of Papain: The Linear Sequence, Active Sulfhydryl Group, and the Disulfide Bridges ,
Proc Natl Acad Sci U S A
52 , 1276 , 1964

Light, A. , Glazer, A. and Smith, E.

Chymotryptic Digest of Papain. III ,
J Biol Chem
235 , 3159 , 1960

Light, A. and Smith, E.

Chymotryptic Digest of Papain. I ,
J Biol Chem
235 , 3144 , 1960

Light, A. and Smith, E.

Chymotryptic Digest of Papain. II ,
J Biol Chem
235 , 3151 , 1960

Lin, W. , Armstrong, D. and Gaucher, G.

Formation and Repair of Papain Sulfenic Acid ,
Can J Biochem
53 , 298 , 1975

Lindahl, P. , Alriksson, E. , Jšrnvall, H. and Bjork, I.

Interaction of the Cysteine Proteinase Inhibitor Chicken Cystatin with Papain ,
Biochemistry
27 , 5074 , 1988

Liu, S. and Hanzlik, R.

The Contribution of Intermolecular Hydrogen Bonding to the Kinetic Specificity of Papain ,
Biochim Biophys Acta
1158 , 264 , 1993

Lowe, G. and Yuthavong, Y.

Kinetic Specificity in Papain-Catalyzed Hydrolyses ,
Biochem J
124 , 107 , 1971

Lowe, G. and Yuthavong, Y.

pH-Dependence and Structure-Activity Relationships in the Papain-Catalyzed Hydrolysis of Anilides ,
Biochem J
124 , 117 , 1971

Lowe, G.

Structural Relationships between Some Plant and Animal Proteases ,
Nature
212 , 1263 , 1966

Luscombe, M. and Phelps, C.

Action of Degradative Enzymes on the Light Fraction of Bovine Septa Protein Polysaccharide ,
Biochem J
103 , 103 , 1967

Mackenzie, N. , Grant, S. , Scott, A. and Malthouse, J.

13C NMR Study of the Sterospecificity of the Thiohemiacetals Formed on Inhibition of Papain by Specific Enantiomeric Aldehyde ,
Biochemistry
25 , 2293 , 1986

Maes, D. , Bouckaert, J. , Poortmans, F. , Wyns, L. and Looze, Y.

Structure of Chymopapain at 1.7 A Resolution ,
Biochemistry
35 , 16292 , 1996

Martin, B. , Feinman, R. and Detwiler, T.

Platelet Stimulation by Thrombin and Other Proteases ,
Biochemistry
14 , 1308 , 1975

Matsumoto, K. , Murata, M. , Sumiya, S. , Kitamura, K. and Ishida, T.

Clarification of Substrate Specificity of Papain by Crystal Analyses of Complexes with Covalent-type Inhibitors ,
Biochim Biophys Acta
1208 , 268 , 1994

McCluskey, R. and Thomas, L.

The Removal of Cartilage Matrix in vivo by Papain. Identification of Crystalline Papain as the Cause of the Phenomenon ,
J Exp Med
108 , 371 , 1958

Migliorini, M. and Creighton, D.

Active Site Ionizations of Papain - An Evaluation of the Potentiometric Difference Titration Method ,
Eur J Biochem
156 , 189 , 1986

Miller, W.

Degradation of Poly-a, L-glutamic Acid. I. Degradation of High Molecular Weight PGA by Papain ,
J Am Chem Soc
83 , 259 , 1961

Mitchel, R. , Chaiden, I. and Smith, E.

The Complete Amino Acid Sequence of Papain. Additions and Corrections ,
J Biol Chem
245 , 3485 , 1970

Mole, J. and Horton, H.

A Kinetic Analysis of the Enhanced Catalytic Efficiency of Papain Modified by 2-Hydroxy-5-Nitrobenzylation ,
Biochemistry
12 , 5285 , 1973

Mole, J. and Horton, H.

Kinetics of Papain-Catalyzed Hydrolysis of a-N-Benzoyl-L-Arginine-p-Nitroanilide ,
Biochemistry
12 , 816 , 1973

Mole, J. and Horton, H.

2-Chloromethyl-4-Nitrophenyl(N-Carbobenzoxy)glucinate. A New Reagent Designed to Introduce an Environmentally Sensitive Conformational Probe Near the Active Site of Papain ,
Biochemistry
12 , 5278 , 1973

Morihara, K.

Chemical Nature of the Active Site of Papain. I. Inhibition by Carbonyl Reagents with Active Methylene Groups ,
J. Biochem.
62 , 250 , 1967

Moussaoui, A. , Nijs, M. , Paul, C. , Wintjens, R. , Vincentelli, J. , Azarkan, M. and Looze, Y.

Revisiting the Enzymes Stored in the Laticifers of Carica papaya in the Context of their Possible Participation in the Plant Defense Mechanism ,
Cell Mol Life Sci
58 , 556 , 2001

Narinesingh, D. and Ngo, T.

Papain Covalently Immobilized on Fractogel Derivative: Preparation, Bioreactor Flow Kinetics, and Stability ,
Biotechnol Appl Biochem
9 , 450 , 1987

Neumann, H. , Shinitzky, M. and Smith, R.

The Activation of Papain and Ficin by Phosphorothioate ,
Biochemistry
6 , 1421 , 1967

Nicklin, M. and Barrett, A.

Inhibition of Cysteine Proteinases and Dipeptidyl Peptidase I by Egg-white Cystatin ,
Biochem J
223 , 245 , 1984

Nolan, C. and Smith, E.

Absence of Sialic Acids and Other Carbohydrates in Crystalline Papain ,
Proc Soc Exp Biol Med
105 , 287 , 1960

O'Hara, B. , Hemmings, A. , Buttle, D. and Pearl, L.

Crystal Structure of Glycyl Endopeptidase from Carica papaya : A Cysteine Endopeptidase of Unusual Substrate Specificity ,
Biochemistry
34 , 13190 , 1995

Ozaki, Y. , Pliura, D. , Carey, P. and Storer, A.

Vibrational Spectra of Scissile Bonds in Enzyme Active Sites: A Resonance Raman Study of Dithioacylpapains ,
Biochemistry
21 , 3102 , 1982

Ozawa, K. , Ohnishi, T. and Tanaka, S.

Activation and Inhibition of Papain ,
J. Biochem.
51 , 372 , 1962

Pickersgill, T. , Rizkallah, P. , Harris, G. and Goodenough, P.

Determination of the Structure of Papaya Protease Omega ,
Acta Crystallogr
47 , 766 , 1991

Pieper, B. and Caliri, M.

Nontraditional Wound Care: A Review of the Evidence for the Use of Sugar, Papaya/Papain, and Fatty Acids ,
J WOCN
30 , 175 , 2003

Polg‡r, L.

Problems of Classification of Papaya Latex Proteinases ,
Biochem J
221 , 555 , 1984

Polg‡r, L.

Isolation of Highly Active Papaya Peptidases A and B from Commercial Chymopapain ,
Biochim Biophys Acta
658 , 262 , 1981

Potter, J. , McCluskey, R. , Weissmann, G. and Thomas, L.

The Removal of Cartilage Matrix by Papain ,
J Exp Med
112 , 1173 , 1960

Revell, D. , Cummings, N. , Baker, K. , Collins, M. , Taylor, M. , Sumner, I. , Pickersgill, R. , Connerton, I. and Goodenough, P.

Nucleotide Sequence and Expression in Escherichia coli of cDNAs Encoding Papaya Proteinase Omega from Carica papaya ,
Gene
127 , 221 , 1993

Saunders, A. and Silverman, L.

Electron Microscopy of Chondromucoprotein and the Products of its Digestion with Hyaluronidase and Papain ,
Nature
214 , 194 , 1967

Schack, P. and Kaarsholm, N.

Absence in Papaya Peptidase A Catalyzed Hydrolysis of a pKa~4 Present in Papain-Catalyzed Hydrolyses ,
Biochemistry
23 , 631 , 1984

Schack, P. and Kaarsholm, N.

Subsite Differences between Active Centres of Papaya Peptidase A and Papain as Revealed by Affinity Chromatography ,
Biochem J
219 , 727 , 1984

Schechter, I. and Berger, A.

On the Size of the Active Site in Proteases. I. Papain ,
Biochem Biophys Res Commun
27 , 157 , 1967

Schneider, M. and Goldstein, G.

Digestion of Articular Cartilage by Papain in Vitro ,
Proc Soc Exp Biol Med
124 , 991 , 1967

Schwert, G. , Neurath, H. , Kaufman, S. and Snoke, J.

The Specific Esterase Activity of Trypsin ,
J Biol Chem
172 , 221 , 1948

Shapira, E. and Arnon, R.

The Mechanism of Inhibition of Papain by Its Specific Antibodies ,
Biochemistry
6 , 3951 , 1967

Shapira, E. and Arnon, R.

Antibodies to Papain. Resolution on DEAE-Sephadex into Fractions with Different Precipitating Capacities ,
Biochim Biophys Acta
140 , 177 , 1967

Shipton, M. , Kierstan, M. , Malthouse, J. , Stuchbury, T. and Brocklehurst, K.

The Case for Assigning a Value of Approximately 4 to pKa1 of Essential Histidine-Cysteine Interactive Systems of Papain, Bromelain and Ficin ,
F.E.B.S. Lett.
50 , 365 , 1975

Skalski, M. , Lewis, S. , Maggio, E. and Shafer, J.

Effects of Proflavine on Papain's Catalytic Activity. A Proflavine-Mediated Decrease in Km ,
Biochemistry
12 , 1884 , 1973

Sluyterman, L.

The Effect of Methanol, Urea, and Other Solutes on the Action of Papain ,
Biochim Biophys Acta
139 , 418 , 1967

Sluyterman, L.

The Activation Reaction of Papain ,
Biochim Biophys Acta
139 , 430 , 1967

Sluyterman, L. and DeGraaf, M.

The Effect of Salts Upon the pH Dependence of the Activity of Papain and Succinyl-Papain ,
Biochim Biophys Acta
258 , 554 , 1972

Sluyterman, L. and Wijdenes, J.

Benzoylamido-acetonitrile as an Inhibitor of Papain ,
Biochim Biophys Acta
302 , 95 , 1973

Sluyterman, L. and Wijdenes, J.

Cyanuration of Papain Activity and Fluorescence of the Products ,
Biochim Biophys Acta
263 , 329 , 1972

Sluyterman, L. and Wijdenes, J.

Sigmoidol Progress Curves in the Polymerization of Leucine Methyl Ester Catalyzed by Papain ,
Biochim Biophys Acta
289 , 194 , 1972

Sluyterman, L.

Reversible Inactivation of Papain by Cyanate ,
Biochim Biophys Acta
139 , 439 , 1967

Smith, E. and Kimmel, J.

, The Enzymes, 2nd Ed. Vol. 4 , P. Boyer, H. Lardy, and K. Myrback , Academic Press, NY , 133 , 1960

Smith, E. , Kimmel, J. and Brown, P.

,
J Biol Chem
207 , 533 , 1954

Smith, L. , Garvin, P. , Gesler, R. and Jennings, R.

Enzyme Dissolution of the Nucleus Pulposus ,
Nature
198 , 1311 , 1963

Solis-Mendiola, S. , Rojo-Dominguez, A. and Hernandez-Arana, A.

Cooperativity in the Unfolding Transitions of Cysteine Proteinases. Calorimetric Study of the Heat Denaturation of Chymopapain and Papain ,
Biochim Biophys Acta
1203 , 121 , 1993

Steiner, R.

Varying Luminescence Behavior of the Different Tryptophan Residues of Papain ,
Biochemistry
10 , 771 , 1971

Storer, A. and Carey, P.

Comparison of the Kinetics and Mechanism of the Papain-Catalyzed Hydrolysis of Esters and Thiono Esters ,
Biochemistry
24 , 6808 , 1985

Storer, A. , Lee, H. and Carey, P.

Relaxed and Perturbed Substrate Conformations in Enzyme Active Sites: Evidence from Multichannel Resonance Raman Spectra ,
Biochemistry
22 , 4789 , 1983

Storer, A. , Murphy, W. and Carey, P.

The Use of Resonance Raman Spectroscopy to Monitor Catalytically Important Bonds during Enzymic Catalysis. Application to the Hydrolysis of Methyl Thionohippurate by Papain ,
J Biol Chem
254 , 3163 , 1979

Storer, A. and Menard, R.

Catalytic Mechanism in Papain Family of Cysteine Pepsidases , Vol. 244 , 486 , 1994

Suzuki, H.

Localized Hyperchondroplasia Induced by Crude Papain Administered to Mice ,
Nature
209 , 1111 , 1966

Taylor, M. , Pratt, K. , Revell, D. , Baker, K. , Sumner, I. and Goodenough, P.

Active Papain Renatured and Processed from Insoluble Recombinant Propapain Expressed in Escherichia coli , Protein Eng Vol. 5 , 455 , 1992

Ungar-Waron, H. , Jaton, J. and Sela, M.

Action of Papain on Normal Rabbit Immunoglobulin M ,
Biochim Biophys Acta
140 , 542 , 1967

Valeri, A. , Wilson, S. and Feinman, R.

Reaction of Antithrombin with Proteinases. Evidence for a Specific Reaction with Papain ,
Biochim Biophys Acta
614 , 526 , 1980

Van Eyk, H.

Fragmentation of Human Globulin with Papain ,
Biochim Biophys Acta
127 , 241 , 1966

Vernet, T. , Berti, P. , de Montigny, C. , Musil, R. , Tessier, D. , Macnard, R. , Magny, M. , Storer, A. and Thomas, D

Processing of the Papain Precursor. The Ionization State of a Conserved Amino Acid Motif within the Pro Region Participates in the Regulation of Intramolecular Processing ,
J Biol Chem
270 , 10838 , 1995

Weinstein, B.

The Evolution of Papain ,
Biochem Biophys Res Commun
41 , 441 , 1970

Westerik, J. and Wolfenden, R.

Aldehydes as Inhibitors of Papain ,
J Biol Chem
247 , 8195 , 1972

Whitehouse, M. and Leader, J.

Biochemical Properties of Anti-Inflammatory Drugs. IX. Coupling of Oxidative Phosphorylation and Inhibition of a Thiol Enzyme (Papain) by Some Cyclic beta-Diones and Ninhydrin ,
Biochem Pharmacol
16 , 537 , 1967

Williams, D. and Whitaker, J.

Kinetics of Papain Catalyzed Hydrolyses of Neutral Substrates ,
Biochemistry
6 , 3711 , 1967

Wilson, A. and Gurner, B.

Increased Affinity of Guinea Pig Thymocytes and Thymus-Dependent Lymphocytes for Papain-Treated Rabbit Erythrocytes Compared to Untreated Erythrocytes ,
J Immunol Methods
7 , 163 , 1975

Wolthers, B. , Dreuth, J. , Jansonius, J. , Koekoek, R. and Swen, H.

The Three Dimensional Structure of Papain , Structure-Function Relationships of Proteolytic Enzymes , P. Desnuelle, H. Neurath, and M. Ottesen , Academic Press, NY , 272 , 1970

Wolthers, B. and Kalk, K.

Binding of Competitive (Product) Inhibitors to Papain in the Active and Inactive State ,
Biochim Biophys Acta
198 , 556 , 1970

Zucker, S. , Buttle, D. , Nicklin, M. and Barrett, A.

The Proteolytic Activities of Chymopapain, Papain and Papaya Proteinase III ,
Biochim Biophys Acta
828 , 196 , 1985

Papain Products

Description

Papain, Suspension

Source:

Carica papaya Latex

Supplied as a two times crystalline suspension in 50mM sodium acetate, pH 4.5. To ensure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.

Store at 2-8°C.

…

Ice Pack required

Activity

Activates to at least 20 units per mg protein

Certificate of Analysis

Code

PAP

Product details

LS003124

25 mg

$40.00

LS003126

100 mg

$88.00

LS003127

1 gm

$595.00

LS003128

Bulk

---

Description

Papain, Lyophilized

Source:

Carica papaya Latex

Supplied as a lyophilized powder prepared from a two times crystalline suspension, Code: PAP. To ensure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.

Store at 2-8°C.

…

Activity

Activates to at least 15 units per mg protein

Certificate of Analysis

Code

PAPL

Product details

LS003118

25 mg

$44.00

LS003119

100 mg

$100.00

LS003120

1 gm

$685.00

LS003122

Bulk

---

Description

PDS Kit, Papain Vial

Source:

Papaya Latex

A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1mM L-cysteine with 0.5mM EDTA.

Store at 2-8°C.

…

Activity

≥100 units per vial

Certificate of Analysis

Code

PAP2

Product details

LK003176

1 vi

$29.00

LK003178

5 vi

$98.00

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Papain References

Papain Products