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NUCxyme™ - Manual
Source:
Komagataella phaffii (previously P. pastoris)
CAS:
9025-65-4
EC:
3.1.30.2
Serratia marcescens Nuclease is a sequence-independent endonuclease with extraordinarily high specific activity. It can completely digest all forms of DNA and RNA (single-stranded, double-stranded, linear, and circular) and produce 5' monophosphate-terminated oligonucleotides of 3-5 bases in length. It is protease and endotoxin-free and extremely useful in bioprocessing applications requiring DNA and/or RNA removal from cellular extracts.
Serratia marcescens nuclease is recombinantly produced in the yeast Komagataella. phaffii to minimize the presence of contaminating nucleases and proteases while eliminating potential pathogens associated with animal-derived materials. This enzyme acts as a non-mammalian source of DNase and RNase for various bioprocessing applications designed to remove nucleic acids and lower the viscosity of cellular extracts.
Characteristics of NUCxyme™
Protein Accession Number
P13717
Molecular Weight
27 kDa
Optimal pH
8-8.5
Stability
Stable for at least six months when stored at -20°C.
Activity
One unit will cause an increase of 0.007 A260 per minute per ml while acting on highly polymerized calf thymus DNA at 25°C and pH 8.0.
NUCxyme™ Products
Description
Activity
Code
Cat. #
Size
Price
Description
NUCxyme™, DNA/RNA Nuclease, Recombinant, AF
Source:
Komagataella phaffii (previously P. pastoris)
Recombinant Serratia marcescens nuclease produced in Komagataella phaffi. Chromatographically purified. Free of animal derived components and proteases. A liquid preparation in 20mM Tris-HCl, 2mM MgCl2, 20mM NaCl, pH 8.0 and 50% glycerol.
Store at -20°C.
Ice Pack required
Code
NUCAR
Product details
LS01522
25 ku
$125.00
LS01524
100 ku
$350.00
LS01525
Bulk
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