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Neutral Protease (Dispase) References

Source:
Bacillus polymyxa
CAS:
9001-92-7
EC:
3.4.24.28
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Akimoto, Y. , Obinata, A. , Endo, H. and Hirano, H.
Immunohistochemical Study of Basement Membrane Reconstruction by an Epidermis-Dermis Recombination Experiment Using Cultured Chick Embryonic Skin: Induction of Tenascin ,
J Histochem Cytochem
 40 , 1129 , 1992
Alvarez, V. , von der Weid, I. , Seldin, L. and Santos, A.
Influence of Growth Conditions on the Production of Extracellular Proteolytic Enzymes in Paenibacillus peoriae NRRL BD-62 and Paenibacillus polymyxa SCE2. ,
Lett Appl Microbiol
 43 , 625-30 , 2006
Ash, C. , Farrow, J. , Wallbanks, S. and Collins, M.
Phylogenetic Heterogeneity of the Genus Bacillus Revealed by Comparative Analysis of Small Subunit-Ribosomal RNA Sequences ,
Lett Appl Microbiol
 13 , 202 , 1991
Ash, C. , Priest, F. and Collins, M.
Molecular Identification of rRNA Group 3 Bacilli (Ash, Farrow, Wallbanks and Collins) Using a PCR Probe Test. Proposal for the Creation of a New Genus Paenibacillus ,
Antonie Van Leeuwenhoek
 64 , 253
De Buysscher, E. , Kennedy, J. and Mendicino, J.
Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis ,
In Vitro
 20 , 433 , 1984
Eijsink, V. , Van Den Burg, B. and Venema, G.
High Performance Affinity Chromatography of Bacillus Neutral Proteases ,
Biotechnol Appl Biochem
 14 , 275-83 , 1991
Feder, J. and Schuck, J.
Studies on the Bacillus subtilis Neutral Protease and Bacillus thermoproteolyticus Thermolysin-Catalyzed Hydrolysis of Dipeptide Substrates ,
Biochemistry
 9 , 2784 , 1970
Feder, J. , Keay, L. , Garrett, L. , Cirulis, N. , Moseley, M. and Wildi, B.
Bacillus cereus Neutral Protease. ,
Biochim Biophys Acta
 251 , 74 , 1971
Fogarty, W. and Griffin, P.
Production and Purification of the Metalloprotease of Bacillus polymyxa ,
Appl Microbiol
 26 , 185 , 1973
Fukumoto, J. and Negoro, H.
Crystallization of Bacterial Proteinase , Proc. Japan Acad. Vol. 27 , 441 , 1951
Garcia, B. , Margolles, E. , Roca, H. , Mateu, D. , Raices, M. , Gonzales, M. , Herrera, L. and Delgado, J.
Cloning and Sequencing of a Dextranase-Encoding cDNA from Penicillium minioluteum ,
FEMS Microbiol Lett
 143 , 175 , 1996
Geggel, H. and Gipson, I.
Removal of Viable Sheets of Conjunctival Epithelium with Dispase II ,
Inv Ophthal Visual Sci
 26 , 15 , 1985
Green, H. , Kehinde, O. and Thomas, J.
Growth of Cultured Human Epidermal Cells into Multiple Epithelia Suitable for Grafting ,
Proc Natl Acad Sci U S A
 76 , 5665 , 1979
Griffin, P. and Fogarty, W.
Physiochemical Properties of the Native, Zinc- and Manganese-Prepared Metalloprotease of Bacillus polymyxa ,
Appl Microbiol
 26 , 191 , 1973
Griffin, P. and Fogarty, W.
Some Properties of a Protease from Bacillus polymyxa ,
Biochem J
 125 , 109P , 1971
Guntelberg, A. and Ottesen, M.
Purification of the Proteolytic Enzyme from Bacillus subtilis ,
C R Trav Lab Carlsberg [Chim]
 29 , 36 , 1954
Hase, C. and Finkelstein, R.
Bacterial Extracellular Zinc-Containing Metalloproteases ,
Microbiol Rev
 57 , 823 , 1993
Hirokawa,K. , Shimoji, K. and Kajiyama, N.
An Enzymatic Method for the Determination of HemoglobinA(1C) ,
Biotechnol Lett
 27 , 963 , 2005
Keay, L. and Wildi, B.
Proteases of the Genus Bacillus . I. Neutral Proteases. ,
Biotechnol Bioeng
 12 , 179 , 1970
Kitano, Y. and Okada, N.
Separation of the Epidermal Sheet by Dispase ,
Br J Dermatol
 108 , 555 , 1983
Koide, Y. , Nakamura, A. , Uozumi, T. and Beppu, T.
Cloning and Sequencing of the Major Intracellular Serine Protease Gene of Bacillus subtilis ,
J Bacteriol
 167 , 110 , 1986
Kubo, M. and Imanaka, T.
Cloning and Nucleotide Sequence of the Highly Thermostable Neutral Protease Gene from Bacillus stearothermophilus ,
J Gen Microbiol
 134 , 1883 , 1988
Matsumura T, Yamanaka T, Hashizume S, Irie Y, Nitta K.
Tissue Dispersion, Cell Harvest and Fluid Suspension Culture by the Use of Bacterial Neutral Protease ,
Jpn J Exp Med
 45 , 377 , 1975
Matsumura, T. , Nitta, K. , Yoshikawa, M. , Takaoka, T. and Katsuta, H.
Action of Bacterial Neutral Protease on the Dispersion of Mammalian Cells in Tissue Culture ,
Jpn J Exp Med
 45 , 383 , 1975
Matsumura, T.
Dispase , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , (Doyle, A., Griffiths, J., and Newell, D., Eds.) , John Wiley and Sons, Ltd. , 3B:2.1-2.7 , 1995
Matsuoka, H. , Okada, M. , Ishii, N. and Maeda, S.
Study of the Effects of Dispase in the Chemotherapy of Multicellular Tumour Spheroids of Small-Cell Lung Carcinoma in Man ,
J Pharm Pharmacol
 50 , 285 , 1998
McConn, J. , Tsuru, D. and Yasunobu, K.
Bacillus subtilis Neutral Proteinase. I. A Zinc Enzyme of High Specific Activity ,
J Biol Chem
 239 , 3706 , 1964
Millet, J. , Acher, R. and Aubert, J.
Biochemical and Physiological Properties of an Extracellular Protease Produced by Bacillus megaterium ,
Biotechnol Bioeng
 11 , 1233 , 1969
Misaki, T. , Yamada, M. , Okazaki, T. and Sawada, J.
Studies on the Protease Constitution of Aspergillus oryzae ,
Agric Biol Chem
 34 , 1383 , 1970
Nagashima, A. , Ando, N. , Sato, M. , Ozawa, S. and Kitajima, M.
Basic Studies on the Application of an Artificial Esophagus Using Cultured Epidermal Cells ,
Surg Today
 27 , 915 , 1997
Nagata, M. and Matsumura, T.
Action of the Bacterial Neutral Protease, Dispase, on Cultured Cells and its Application to Fluid Suspension Culture with a Review on Biomedical Application of this Protease ,
Jpn J Exp Med
 56 , 297-307 , 1986
Nomoto, M. and Narahashi, Y.
A Proteolytic Enzyme of Streptomyces griseus . IV. General Properties of Streptomyces griseus Protease ,
J. Biochem.
 46 , 1645 , 1959
Ono, J. , Takaki, R. and Fukuma, M.
Preparation of Single Cells from Pancreatic Islets of Adult Rat by the Use of Dispase. ,
Endocrinology
 24 , 265 , 1977
Ottesen, M. and Spector, A.
A Comparison of Two Proteinases from Bacillus subtilis ,
C R Trav Lab Carlsberg [Chim]
 32 , 63 , 1960
Roberts G. and Brunt, J.
Identification of an Epidermal Cell-Adhesion Glycoprotein ,
Biochem J
 232 , 67 , 1985
Saul, D. , Williams, L. , Toogood, H. , Daniel, R. and Bergquist, P.
Sequence of the Gene Encoding a Highly Thermostable Neutral Proteinase from Bacillus sp. strain EA1: Expression in Escherichia coli and Characterisation ,
Biochim Biophys Acta
 1308 , 74 , 1996
Schaefer, B. , Wallich, R. , Schmolke, K. , Fink, W. , Bechtel, M. , Reinartz, J. and Kramer, M.
Immunohistochemical and Molecular Characterization of Cultured Keratinocytes after Dispase-Mediated Detachment from the Growth Substratum ,
Exp Dermatol
 9 , 58 , 2000
Stark, W. , Pauptit, R. , Wilson, K. and Jansonius, J.
The Structure of Neutral Protease from Bacillus cereus at 0.2-nm resolution. ,
Eur J Biochem
 207 , 781-91 , 1992
Stenn, K. , Link, R. , Moellmann, G. , Madri, J. and Kuklinska, E.
Dispase, a Neutral Protease from Bacillus polymyxa , is a Powerful Fibronectinase and Type IV Collagenase ,
J Invest Dermatol
 93 , 287 , 1989
Stockle, G. , Wahl, R. and Seif, F.
Micromethod of Human Thyrocyte Cultures for Detection of Thyroid-Stimulating Antibodies and Thyrotrophin ,
Acta Endocrinol (Copenh)
 97 , 369 , 1981
Takekawa, S. , Uozumi, N. , Tsukagoshi, N. and Udaka, S.
Proteases Involved in Generation of Beta- and Alpha-Amylases from a Large Amylase Precursor in Bacillus polymyxa ,
J Bacteriol
 173 , 6820 , 1991
Tezel, T. , Del Priore, L. and Kaplan, H.
Posterior Vitreous Detachment with Dispase ,
Retina
 18 , 7 , 1998
Tsuru, D. , McConn, J. and Yasunobu, K.
Bacillus subtilis Neutral Proteinase. II. Some Physiochemical Properties ,
J Biol Chem
 240 , 2415 , 1965
Van Dorp, A. , Verhoeven, M. , Nat-Van Der Meij, T. , Koerten, H. and Ponec, M.
A Modified Culture System for Epidermal Cells for Grafting Purposes: An in vitro and in vivo Study ,
Wound Repair Regen
 7 , 214 , 1999
von der Weid, I. , Duarte, G. , van Elsas, J. and Seldin, L.
Paenibacillus brasilensis sp. nov.: A Novel Nitrogen-Fixing Species Isolated from the Maize Rhizosphere in Brazil ,
Int J Syst Evol Microbiol
 52 , 2147 , 2002

Neutral Protease (Dispase) Products

Description
Activity
Code
Cat. #
Size
Price
Description
Neutral Protease (Dispase), Purified, AF
Source:
Bacillus polymyxa
Animal Free/AF. Chromatographically purified. A lyophilized powder.
Store at 2-8°C.
…
Activity
≥4 units per mg dry weight
Code
NPRO
LS02100
10 mg
$84.00
LS02104
50 mg
$352.00
LS02106
250 mg
$1,575.00
LS02108
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 5000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Neutral Protease, Partially Purified, AF
Source:
Bacillus polymyxa
Animal Free/AF. Partially purified. A lyophilized powder.
Store at 2-8°C.
…
Activity
≥0.1 units per mg dry weight
Code
NPRO2
LS02110
100 mg
$39.00
LS02109
1 gm
$171.00
LS02111
5 gm
$795.00
LS02112
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 100000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
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