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Lactate Dehydrogenase - Assay
Source:
Recombinant protein produced in E. coli
CAS:
9001-60-9
EC:
1.1.1.27
Lactate Dehydrogenase Assay
Method
The reaction velocity is determined by a decrease in absorbance at 340 nm resulting from the oxidation of NADH. One unit causes the oxidation of one micromole of NADH per minute at 25°C and pH 7.3, under the specified conditions.
Reagents
- 0.2 M Tris⋅HCl, pH 7.3
- 6.6 mM NADH in above 0.2 M Tris⋅HCl buffer, pH 7.3
- 30 mM Sodium pyruvate in above 0.2 Tris⋅HCl buffer, pH 7.3
Enzyme
Dissolve at 1 mg/ml in 0.2 M Tris⋅HCl buffer. Dilute enzyme prior to use to obtain a rate of 0.02-0.04 ΔA/min. in Tris buffer and keep cold.
Determination of Protein Concentration:
Procedure
Set spectrophotometer at 340 nm and 25°C.
Pipette into cuvette as follows:
Tris⋅HCl, 0.2 M pH 7.3 2.8 ml 6.6 mM NADH 0.1 ml 30 mM Sodium pyruvate 0.1 ml
Incubate in the spectrophotometer 4-5 minutes to achieve temperature equilibration and establish a blank rate, if any.
Add 0.1 ml of appropriately diluted enzyme and record ΔA340/min from initial linear portion.
Calculation
Lactate Dehydrogenase Products
Description
Activity
Code
Cat. #
Size
Price
Description
Lactate Dehydrogenase, Lyophilized, Recombinant Rabbit Muscle
Source:
E. coli
Recombinant rabbit muscle Lactate Dehydrogenase produced in E.Coli. Chromatographically purified. A lyophilized powder.
Store at -20°C.
Code
LADCL
Product details
LS002755
5 ku
$130.00
LS002756
25 ku
$600.00
LS002757
Bulk
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