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Hyaluronidase - Manual
Source:
Bovine Testes
CAS:
37326-33-3
EC:
3.2.1.35
Hyaluronidase catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido-2-deoxy-b-D-glucose and D-glucose residues in hyaluronate.
Characteristics of Hyaluronidase from Bovine Testes:
Specificity
Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B), primarily to tetrasaccharide residues (Ludowieg et al. 1961). Monosaccharides are not liberated (Rappaport et al. 1951).
Composition
The enzyme is a glycoprotein containing 5% mannose and 2.17% glucosamine. The amino acid composition has been determined ( Borders and Raftery 1968).
Characteristics of Hyaluronidase
Molecular Weight
Borders and Raftery (1968) reported a molecular weight of 61,000. Khorlin et al. (1973) report four subunits of 14,000 each and a total molecular weight of 55,000.
Optimal pH
4.5 - 6 (DeSalequi et al. 1967).
Extinction Coefficient
= approximately 8.
Inhibitors
Fe2+ and Fe3+ are inhibitory as are Mn2+ and Cu2+ (Warren et al. 1962).
Stability
Worthington hyaluronidase is stable for 1 - 2 years and the purified preparation for 6 - 12 months when stored at -20°C.
Stabilizers
Yang and Srivastav (1975) report that sodium chloride acts as a stabilizer.
Hyaluronidase Products
Description
Activity
Code
Cat. #
Size
Price
Description
Hyaluronidase, Purified
Source:
Bovine Testes
Chromatographically purified. A dialyzed, lyophilized powder.
Store at -20°C.
Code
HSEP
Product details
LS005477
5 ku
$93.00
LS005475
15 ku
$230.00
LS005474
30 ku
$368.00
LS005479
Bulk
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Description
Hyaluronidase
Source:
Bovine Testes
A partially purified, dialyzed, lyophilized powder.
Store at -20°C.
Code
HSE
Product details
LS002594
50 ku
$58.00
LS002592
300 ku
$240.00
LS002591
Bulk
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