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Glucuronidase, Beta - Manual
CAS:
9001-45-0
EC:
3.2.1.31
β-Glucuronidase hydrolyzes conjugated glucuronides.
It is ubiquitious in animal lysozymes.
The enzyme has found wide application in determining urinary steroids.
Characteristics of β-glucuronidase from Bovine Liver:
Specificity
The enzyme hydrolyzes a large variety of conjugated glucuronides but not α- or β-glucosides. See report by Tomasic and Keglevic (1973).
Composition
It is a sialic acid containing glycoprotein. The amino acid and carbohydrate content have been reported by Himeno et al. (1974). It is probable that the heterogeneity reported by Plapp and Cole (1967) is due to differing carbohydrate content. Wang and Touster (1972) report on an active center study using the rat liver enzyme which may be similar to that from beef liver.
Characteristics of Glucuronidase, Beta
Molecular Weight
290,000 (Himeno et al. 1974).
Optimal pH
4.4 (Wang and Touster 1972).
Isoelectric point
5.1 (Himeno et al. 1974).
Extinction Coefficient
= 17.0 (Plapp and Cole 1966).
Inhibitors
It is strongly, reversibly inhibited by various organic peroxides (Christner et al. 1970).
Stability
Lyophilized, Worthington glucuronidase is stable 6 - 12 months at 2 - 8°C.