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Cholinesterase, Butyryl - Manual

Source:
Horse Serum
CAS:
9001-08-5
EC:
3.1.1.8
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Acylcholine acylhydrolase

Butyryl cholinesterase, (ButChE), catalyzes the hydrolysis of a number of choline esters:

formula

The enzyme is found in mammalian blood plasma, liver, pancreas, intestinal mucosa and the white matter of the central nervous system. It is sometimes referred to as serum cholinesterase as opposed to red cell cholinesterase (AChE). It hydrolyzes butyrylcholine 4 times more rapidly than acetylcholine. ChE does not hydrolyze D-β-methyl acetylcholine whereas AChE does. Both are inhibited by 10-5 M physostigmine. Because the enzyme is so markedly inhibited by organophosphate compounds used as insecticides and neurotoxins, it is widely used in monitoring systems. An immobilized cholinesterase detection device has been described by Goodson et al. (1973).

Characteristics of Butyrylcholinesterase from Horse Serum:

Specificity

The enzyme is more active with butyryl and propionyl choline than with acetyl choline. See also Main et al. (1974) for some comparative activities. Non-choline esters (e.g., procaine, morphine esters, atropine and cocaine) are susceptible to the action of ButChE (Augustinsson 1960).

Composition

Lee and Harpst (1973) indicate ButChE to be a tetrameric structure with equally sized subunits of 110,000 daltons. It is a glycoprotein. Main et al. (1974) have determined the amino acid and carbohydrate composition, based upon a particle weight of 101,000. See also Chiu et al. (1972) and Pavlic (1972).

Characteristics of Cholinesterase, Butyryl
Molecular Weight

440,000 (Lee and Harpst 1973; see also Main et al. 1974)

Optimal pH

6.0-8.0 (Augustinsson 1960)

Extinction Coefficient

E280 =13.6 (Main et al. 1974)

Activators

Ca2+ and Mg2+ (Augustinsson 1960)

Inhibitors

Numerous organophosphate esters, the carbamate derivatives and quaternary ammonium salts (Augustinsson 1960). See also Kamaric (1975), Koelle et al. (1974), Millner et al. (1974), Stanley et al. (1974), Ashani et al. (1972), Post (1971).

Cholinesterase, Butyryl Products

Description
Activity
Code
Cat. #
Size
Price
Description
Cholinesterase, Butyryl
Source:
Horse Serum
A partially purified lyophilized powder.
Store at 2-8°C.
…
Activity
≥4 units per mg dry weight
Code
CHE
LS001628
500 un
$335.00
LS001632
4 ku
$2,180.00
LS001636
Bulk
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The minimum amount for bulk packaging/pricing for this product is 80000 x 1 un. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.