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Aldolase - Assay
Source:
Rabbit Muscle
CAS:
9024-52-6
EC:
4.1.2.13
Aldolase Assay
Method
Based on Boyer's modification of the hydrazine assay (Jagannathan et al. 1956) in which 3-phosphoglyceraldehyde reacts with hydrazine to form a hydrazone which absorbs at 240 nm. The advantages of the system are its simplicity and specificity. One unit is described as a change in absorbance of 1.00 per minute at 25°C and pH 7.5 under the specified conditions. Alternate methods for the assay of aldolase have been described by Rutter (1961). The coupled a-glycerophosphate dehydrogenase assay (Richards et al. 1961) has been used.
Reagents
- 0.0001 M EDTA, pH 7.5 containing 0.0035 M hydrazine sulfate
- Substrate: 0.012 M Fructose-1,6-bisphosphate pH 7.5
Enzyme
Immediately before use, dilute in water to a concentration of 0.5-2.0 units per ml.
Procedure
Set spectrophotometer at 240 nm and 25°C.
Pipette into cuvettes as follows:
Blank Test Fructose-1,6-bisphosphate 1.0 ml 1.0 ml Hydrazine sulfate 2.0 ml 2.0 ml Distilled water 0.1 ml ---- Record ΔA240 for 10 minutes Add enzyme ---- 0.1 ml Record ΔA240 for 10 minutes
Subtract ΔA240/min of the blank from ΔA240/min of the test.
Calculation
Aldolase Products
Description
Activity
Code
Cat. #
Size
Price
Description
Aldolase, Suspension
Source:
Rabbit Muscle
Two times crystallized. A suspension in 2.1M ammonium sulfate, pH 7.8.
Store at 2-8°C.
Code
ALD
Product details
LS001123
100 mg
$150.00
LS001125
Bulk
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Description
Aldolase, Lyophilized
Source:
Rabbit Muscle
Chromatographically purified. An inactive lyophilized powder containing 80% sucrose by weight. Purity checked by SDS PAGE. Useful as a chromatography size marker.
Store at 2-8°C. PROTECT FROM MOISTURE.
Code
ALDC
Product details
LS001130
100 mg
$185.00
LS001128
Bulk
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