Quick Order

Aldolase - Assay

Source:
Rabbit Muscle
CAS:
9024-52-6
EC:
4.1.2.13
Shop now

Aldolase Assay

Method
Based on Boyer's modification of the hydrazine assay (Jagannathan et al. 1956) in which 3-phosphoglyceraldehyde reacts with hydrazine to form a hydrazone which absorbs at 240 nm. The advantages of the system are its simplicity and specificity. One unit is described as a change in absorbance of 1.00 per minute at 25°C and pH 7.5 under the specified conditions. Alternate methods for the assay of aldolase have been described by Rutter (1961). The coupled a-glycerophosphate dehydrogenase assay (Richards et al. 1961) has been used.
Reagents

 

  • 0.0001 M EDTA, pH 7.5 containing 0.0035 M hydrazine sulfate
  • Substrate: 0.012 M Fructose-1,6-bisphosphate pH 7.5
Enzyme

Immediately before use, dilute in water to a concentration of 0.5-2.0 units per ml.

equation

Procedure

Set spectrophotometer at 240 nm and 25°C.

Pipette into cuvettes as follows:

  Blank Test
Fructose-1,6-bisphosphate 1.0 ml 1.0 ml
Hydrazine sulfate 2.0 ml 2.0 ml
Distilled water 0.1 ml ----
Record ΔA240 for 10 minutes    
Add enzyme ---- 0.1 ml
Record ΔA240 for 10 minutes    

Subtract ΔA240/min of the blank from ΔA240/min of the test.

Calculation

Aldolase Products

Description
Activity
Code
Cat. #
Size
Price
Description
Aldolase, Suspension
Source:
Rabbit Muscle
Two times crystallized. A suspension in 2.1M ammonium sulfate, pH 7.8.
Store at 2-8°C.
…
Activity
≥10 units per mg protein
Code
ALD
LS001123
100 mg
$144.00
LS001125
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 20000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.
Description
Aldolase, Lyophilized
Source:
Rabbit Muscle
Chromatographically purified. An inactive lyophilized powder containing 80% sucrose by weight. Purity checked by SDS PAGE. Useful as a chromatography size marker.
Store at 2-8°C. PROTECT FROM MOISTURE.
…
Activity
---
Code
ALDC
LS001130
100 mg
$176.00
LS001128
Bulk
---
The minimum amount for bulk packaging/pricing for this product is 2000 x 1 mg. Please contact custservice@worthington-biochem.com to request a quote for smaller amounts.