Worthington Announces New Products for Nucleic Acid Research and Bioprocessing

Worthington Biochemical (WBC) announced the availability of two new products for nucleic acid research and bioprocessing applications. 

• NUCxyme™ DNA/RNA Nuclease, Recombinant,     Animal Free

Recombinant Serratia marcescens Nuclease, Animal Free

Serratia marcescens nuclease is recombinantly produced in the yeast Komagataella phaffii to minimize the presence of contaminating nucleases and proteases while eliminating potential pathogens associated with animal-derived materials. This enzyme acts as a non-mammalian source of DNase and RNase for various bioprocessing applications designed to remove nucleic acids and lower the viscosity of cellular extracts.

Serratia marcescens nuclease is a sequence-independent endonuclease with extraordinarily high specific activity. It can completely digest all forms of DNA and RNA (single-stranded, double-stranded, linear, and circular) and produce 5' monophosphate-terminated oligonucleotides of 3-5 bases in length. It is protease and endotoxin-free and extremely useful in bioprocessing applications requiring DNA and/or RNA removal from cellular extracts.

• Ribonuclease U2

 Ustilago sphaerogena ribonuclease U2 produced recombinantly in yeast (Komagataella phaffi-previously P. pastoris) to decrease contaminant nucleases and proteases and eliminate potential pathogens associated with animal-sourced materials.

Ribonuclease U2 is an endonuclease that hydrolyzes the phosphodiester bonds of 3’-linked ribonucleotides, resulting in nucleoside 3’-phosphates and 3’-phosphooligonucleotides ending in Ap or Gp with 2’, 3’-cyclic intermediates. It is purine selective with a preference for adenosine and has the following order of cleavage specificity (A>G>C>U). Because of this, it is helpful in RNA sequencing and RNA modification and mapping applications. Optimal activity is observed at pH 4.5.

About Worthington Biochemical Corporation

Founded in 1947, Worthington Biochemical Corporation is celebrating over 75 years as an industry leader in the development and primary production of high-quality purified enzymes, proteins, nucleic acids and cell isolation kits for life science research, diagnostics and biotechnology applications. An ISO9001 Certified, primary manufacturer Worthington meets enzyme requirements from research to larger scale bulk bioprocessing and OEM applications. Worthington Biochemical Corporation is a privately held company, headquartered in Lakewood, NJ and has worldwide distribution through a network of distributors. For more information go to: Worthington-Biochem.com

For more information, contact: custservice@worthington-biochem.com